Restriction map for
pF1KSDA1951
(Linear) (Six-base) MAPSORT of: KIAA1951.seq Check: 7785 from: 1 to: 2010
KIAA1951.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1323 1478 2010
Size: 1323 155 532
Fragments arranged by size:
1323 532 155
AccI GT'mk_AC
Cuts at: 0 1088 2010
Size: 1088 922
AfeI AGC'GCT
Cuts at: 0 1606 2010
Size: 1606 404
AleI CACnn'nnGTG
Cuts at: 0 996 1123 1382 1418 1484 1511 2010
Size: 996 127 259 36 66 27 499
Fragments arranged by size:
996 499 259 127 66 36 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 257 509 1400 1486 2010
Size: 257 252 891 86 524
Fragments arranged by size:
891 524 257 252 86
AlwNI CAG_nnn'CTG
Cuts at: 0 441 1920 2010
Size: 441 1479 90
Fragments arranged by size:
1479 441 90
ApaI G_GGCC'C
Cuts at: 0 320 978 1143 1400 2010
Size: 320 658 165 257 610
Fragments arranged by size:
658 610 320 257 165
ApaLI G'TGCA_C
Cuts at: 0 1571 1872 2010
Size: 1571 301 138
Fragments arranged by size:
1571 301 138
AvaI C'yCGr_G
Cuts at: 0 454 1787 2010
Size: 454 1333 223
Fragments arranged by size:
1333 454 223
BanI G'GyrC_C
Cuts at: 0 300 649 1242 1376 2010
Size: 300 349 593 134 634
Fragments arranged by size:
634 593 349 300 134
BanII G_rGCy'C
Cuts at: 0 320 368 587 816 861 978 1143 1286
Size: 320 48 219 229 45 117 165 143
Cuts at: 1286 1400 1621 1794 1985 2010
Size: 114 221 173 191 25
Fragments arranged by size:
320 229 221 219 191 173 165 143
117 114 48 45 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1011 2010
Size: 1011 999
BbvCI CC'TCA_GC
Cuts at: 0 17 311 849 1356 1543 2010
Size: 17 294 538 507 187 467
Fragments arranged by size:
538 507 467 294 187 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1150 1184 2010
Size: 1150 34 826
Fragments arranged by size:
1150 826 34
BlpI GC'TnA_GC
Cuts at: 0 506 2010
Size: 506 1504
Bme1580I G_kGCm'C
Cuts at: 0 320 652 978 1143 1245 1400 1575 1876
Size: 320 332 326 165 102 155 175 301
Cuts at: 1876 2010
Size: 134
Fragments arranged by size:
332 326 320 301 175 165 155 134
102
BmgBI CAC'GTC
Cuts at: 0 1576 2010
Size: 1576 434
BmrI ACTGGGnnnn_n'
Cuts at: 0 1969 2010
Size: 1969 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 307 1259 1832 2010
Size: 226 81 952 573 178
Fragments arranged by size:
952 573 226 178 81
Bpu10I CC'TnA_GC
Cuts at: 0 17 311 815 849 1356 1543 2010
Size: 17 294 504 34 507 187 467
Fragments arranged by size:
507 504 467 294 187 34 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1948 2010
Size: 1060 888 62
Fragments arranged by size:
1060 888 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1702 1882 2010
Size: 1702 180 128
Fragments arranged by size:
1702 180 128
BsaAI yAC'GTr
Cuts at: 0 1458 2010
Size: 1458 552
BsaBI GATnn'nnATC
Cuts at: 0 1824 2010
Size: 1824 186
BsaHI Gr'CG_yC
Cuts at: 0 1410 2010
Size: 1410 600
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 646 676 2010
Size: 646 30 1334
Fragments arranged by size:
1334 646 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 271 643 691 709 712 730 766
Size: 268 3 372 48 18 3 18 36
Cuts at: 766 2010
Size: 1244
Fragments arranged by size:
1244 372 268 48 36 18 18 3
3
BseYI C'CCAG_C
Cuts at: 0 425 482 809 836 1269 1319 1881 1913
Size: 425 57 327 27 433 50 562 32
Cuts at: 1913 2010
Size: 97
Fragments arranged by size:
562 433 425 327 97 57 50 32
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1406 1940 2010
Size: 1406 534 70
Fragments arranged by size:
1406 534 70
BsiHKAI G_wGCw'C
Cuts at: 0 178 368 607 1342 1575 1621 1794 1876
Size: 178 190 239 735 233 46 173 82
Cuts at: 1876 2010
Size: 134
Fragments arranged by size:
735 239 233 190 178 173 134 82
46
BsmI GAATG_Cn'
Cuts at: 0 340 2010
Size: 340 1670
Bsp1286I G_dGCh'C
Cuts at: 0 178 320 368 587 607 652 816 861
Size: 178 142 48 219 20 45 164 45
Cuts at: 861 978 1143 1245 1286 1342 1400 1575 1621
Size: 117 165 102 41 56 58 175 46
Cuts at: 1621 1794 1876 1985 2010
Size: 173 82 109 25
Fragments arranged by size:
219 178 175 173 165 164 142 117
109 102 82 58 56 48 46 45
45 41 25 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1015 1323 1478 1634 2010
Size: 1015 308 155 156 376
Fragments arranged by size:
1015 376 308 156 155
BsrDI GCAATG_nn'
Cuts at: 0 690 1156 2010
Size: 690 466 854
Fragments arranged by size:
854 690 466
BsrFI r'CCGG_y
Cuts at: 0 863 872 953 1133 1214 1228 1379 1637
Size: 863 9 81 180 81 14 151 258
Cuts at: 1637 2010
Size: 373
Fragments arranged by size:
863 373 258 180 151 81 81 14
9
BsrGI T'GTAC_A
Cuts at: 0 1000 2010
Size: 1000 1010
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1050 2010
Size: 237 813 960
Fragments arranged by size:
960 813 237
BstXI CCAn_nnnn'nTGG
Cuts at: 0 985 1513 2010
Size: 985 528 497
Fragments arranged by size:
985 528 497
BstYI r'GATC_y
Cuts at: 0 410 2010
Size: 410 1600
Bsu36I CC'TnA_GG
Cuts at: 0 626 2010
Size: 626 1384
BtgI C'CryG_G
Cuts at: 0 758 1098 1751 2010
Size: 758 340 653 259
Fragments arranged by size:
758 653 340 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1019 1045 1147 1878 2010
Size: 1019 26 102 731 132
Fragments arranged by size:
1019 731 132 102 26
BtsI GCAGTG_nn'
Cuts at: 0 62 544 915 1145 2010
Size: 62 482 371 230 865
Fragments arranged by size:
865 482 371 230 62
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 509 544 2010
Size: 509 35 1466
Fragments arranged by size:
1466 509 35
EaeI y'GGCC_r
Cuts at: 0 23 1095 1535 1654 1734 2010
Size: 23 1072 440 119 80 276
Fragments arranged by size:
1072 440 276 119 80 23
EarI CTCTTCn'nnn_
Cuts at: 0 154 698 731 2010
Size: 154 544 33 1279
Fragments arranged by size:
1279 544 154 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 891 936 2010
Size: 891 45 1074
Fragments arranged by size:
1074 891 45
EcoICRI GAG'CTC
Cuts at: 0 366 1619 1792 2010
Size: 366 1253 173 218
Fragments arranged by size:
1253 366 218 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 307 1259 1832 2010
Size: 226 81 952 573 178
Fragments arranged by size:
952 573 226 178 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1250 2010
Size: 1250 760
EcoO109I rG'GnC_Cy
Cuts at: 0 132 316 317 1397 1493 2010
Size: 132 184 1 1080 96 517
Fragments arranged by size:
1080 517 184 132 96 1
FspI TGC'GCA
Cuts at: 0 1462 2010
Size: 1462 548
HaeII r_GCGC'y
Cuts at: 0 1608 1927 2010
Size: 1608 319 83
Fragments arranged by size:
1608 319 83
HincII GTy'rAC
Cuts at: 0 68 101 2010
Size: 68 33 1909
Fragments arranged by size:
1909 68 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1646 1973 2010
Size: 671 975 327 37
Fragments arranged by size:
975 671 327 37
MscI TGG'CCA
Cuts at: 0 1537 2010
Size: 1537 473
MslI CAynn'nnrTG
Cuts at: 0 380 983 996 1123 1382 1418 1484 1511
Size: 380 603 13 127 259 36 66 27
Cuts at: 1511 1568 2010
Size: 57 442
Fragments arranged by size:
603 442 380 259 127 66 57 36
27 13
MspA1I CmG'CkG
Cuts at: 0 805 1100 1279 1323 1418 1557 1739 1776
Size: 805 295 179 44 95 139 182 37
Cuts at: 1776 1944 2010
Size: 168 66
Fragments arranged by size:
805 295 182 179 168 139 95 66
44 37
NaeI GCC'GGC
Cuts at: 0 865 1230 2010
Size: 865 365 780
Fragments arranged by size:
865 780 365
NcoI C'CATG_G
Cuts at: 0 758 1751 2010
Size: 758 993 259
Fragments arranged by size:
993 758 259
NgoMIV G'CCGG_C
Cuts at: 0 863 1228 2010
Size: 863 365 782
Fragments arranged by size:
863 782 365
NspI r_CATG'y
Cuts at: 0 240 2010
Size: 240 1770
PasI CC'CwG_GG
Cuts at: 0 358 2010
Size: 358 1652
PflMI CCAn_nnn'nTGG
Cuts at: 0 1590 1920 2010
Size: 1590 330 90
Fragments arranged by size:
1590 330 90
PmlI CAC'GTG
Cuts at: 0 1458 2010
Size: 1458 552
PspOMI G'GGCC_C
Cuts at: 0 316 974 1139 1396 2010
Size: 316 658 165 257 614
Fragments arranged by size:
658 614 316 257 165
PstI C_TGCA'G
Cuts at: 0 922 962 1631 1943 2010
Size: 922 40 669 312 67
Fragments arranged by size:
922 669 312 67 40
PvuII CAG'CTG
Cuts at: 0 805 1323 1557 1944 2010
Size: 805 518 234 387 66
Fragments arranged by size:
805 518 387 234 66
SacI G_AGCT'C
Cuts at: 0 368 1621 1794 2010
Size: 368 1253 173 216
Fragments arranged by size:
1253 368 216 173
SacII CC_GC'GG
Cuts at: 0 1101 2010
Size: 1101 909
SfcI C'TryA_G
Cuts at: 0 918 958 1627 1807 1939 2010
Size: 918 40 669 180 132 71
Fragments arranged by size:
918 669 180 132 71 40
SgrAI Cr'CCGG_yG
Cuts at: 0 872 1133 1214 1379 1637 2010
Size: 872 261 81 165 258 373
Fragments arranged by size:
872 373 261 258 165 81
SmlI C'TyrA_G
Cuts at: 0 1039 1927 2010
Size: 1039 888 83
Fragments arranged by size:
1039 888 83
StyI C'CwwG_G
Cuts at: 0 758 1013 1751 2010
Size: 758 255 738 259
Fragments arranged by size:
758 738 259 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 453 667 919 2010
Size: 453 214 252 1091
Fragments arranged by size:
1091 453 252 214
TatI w'GTAC_w
Cuts at: 0 1000 1389 2010
Size: 1000 389 621
Fragments arranged by size:
1000 621 389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 646 678 2010
Size: 646 32 1332
Fragments arranged by size:
1332 646 32
Enzymes that do cut:
AarI AccI AfeI AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbsI BbvCI BcgI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII HincII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PmlI PspOMI PstI PvuII SacI SacII SfcI SgrAI
SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BclI BfrBI BglI BglII BmtI BplI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI
EagI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI