Restriction map for
pF1KA1953
(Linear) (Six-base) MAPSORT of: KIAA1953.seq Check: 6166 from: 1 to: 2826
KIAA1953.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1583 2826
Size: 1583 1243
AatII G_ACGT'C
Cuts at: 0 791 2826
Size: 791 2035
AccI GT'mk_AC
Cuts at: 0 172 2826
Size: 172 2654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1314 1642 2590 2826
Size: 254 1060 328 948 236
Fragments arranged by size:
1060 948 328 254 236
AgeI A'CCGG_T
Cuts at: 0 1938 2826
Size: 1938 888
AhdI GACnn_n'nnGTC
Cuts at: 0 100 2826
Size: 100 2726
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 866 2236 2826
Size: 541 325 1370 590
Fragments arranged by size:
1370 590 541 325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 235 267 2826
Size: 235 32 2559
Fragments arranged by size:
2559 235 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 540 1821 2510 2535 2826
Size: 132 408 1281 689 25 291
Fragments arranged by size:
1281 689 408 291 132 25
ApaI G_GGCC'C
Cuts at: 0 1128 1815 2576 2826
Size: 1128 687 761 250
Fragments arranged by size:
1128 761 687 250
ApaLI G'TGCA_C
Cuts at: 0 841 1381 2826
Size: 841 540 1445
Fragments arranged by size:
1445 841 540
ApoI r'AATT_y
Cuts at: 0 157 758 1609 2826
Size: 157 601 851 1217
Fragments arranged by size:
1217 851 601 157
AvaI C'yCGr_G
Cuts at: 0 105 1268 1280 2107 2266 2826
Size: 105 1163 12 827 159 560
Fragments arranged by size:
1163 827 560 159 105 12
BanI G'GyrC_C
Cuts at: 0 494 2178 2448 2826
Size: 494 1684 270 378
Fragments arranged by size:
1684 494 378 270
BanII G_rGCy'C
Cuts at: 0 1128 1762 1815 2407 2576 2825 2826
Size: 1128 634 53 592 169 249 1
Fragments arranged by size:
1128 634 592 249 169 53 1
BbeI G_GCGC'C
Cuts at: 0 498 2452 2826
Size: 498 1954 374
Fragments arranged by size:
1954 498 374
BbsI GAAGACnn'nnnn_
Cuts at: 0 242 1197 1630 2567 2826
Size: 242 955 433 937 259
Fragments arranged by size:
955 937 433 259 242
BbvCI CC'TCA_GC
Cuts at: 0 1867 2826
Size: 1867 959
BclI T'GATC_A
Cuts at: 0 2133 2826
Size: 2133 693
BglI GCCn_nnn'nGGC
Cuts at: 0 1641 2826
Size: 1641 1185
BglII A'GATC_T
Cuts at: 0 2257 2826
Size: 2257 569
BlpI GC'TnA_GC
Cuts at: 0 553 1756 2826
Size: 553 1203 1070
Fragments arranged by size:
1203 1070 553
Bme1580I G_kGCm'C
Cuts at: 0 845 1128 1385 1815 2181 2576 2826
Size: 845 283 257 430 366 395 250
Fragments arranged by size:
845 430 395 366 283 257 250
BmgBI CAC'GTC
Cuts at: 0 564 1323 2826
Size: 564 759 1503
Fragments arranged by size:
1503 759 564
BmrI ACTGGGnnnn_n'
Cuts at: 0 2653 2826
Size: 2653 173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 619 1037 1124 1360 1375 1706 1749
Size: 595 24 418 87 236 15 331 43
Cuts at: 1749 1918 2377 2826
Size: 169 459 449
Fragments arranged by size:
595 459 449 418 331 236 169 87
43 24 15
Bpu10I CC'TnA_GC
Cuts at: 0 1867 2068 2826
Size: 1867 201 758
Fragments arranged by size:
1867 758 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1972 2826
Size: 1234 738 854
Fragments arranged by size:
1234 854 738
BsaI GGTCTCn'nnnn_
Cuts at: 0 1175 2133 2826
Size: 1175 958 693
Fragments arranged by size:
1175 958 693
BsaAI yAC'GTr
Cuts at: 0 1161 1581 2826
Size: 1161 420 1245
Fragments arranged by size:
1245 1161 420
BsaBI GATnn'nnATC
Cuts at: 0 2039 2826
Size: 2039 787
BsaHI Gr'CG_yC
Cuts at: 0 495 788 2449 2826
Size: 495 293 1661 377
Fragments arranged by size:
1661 495 377 293
BsaWI w'CCGG_w
Cuts at: 0 1013 1184 1785 1938 2826
Size: 1013 171 601 153 888
Fragments arranged by size:
1013 888 601 171 153
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 299 329 1333 1363 2300 2330 2826
Size: 299 30 1004 30 937 30 496
Fragments arranged by size:
1004 937 496 299 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1138 1402 1405 1435 1915 1973 2826
Size: 493 645 264 3 30 480 58 853
Fragments arranged by size:
853 645 493 480 264 58 30 3
BseYI C'CCAG_C
Cuts at: 0 12 68 442 533 1474 2348 2523 2575
Size: 12 56 374 91 941 874 175 52
Cuts at: 2575 2826
Size: 251
Fragments arranged by size:
941 874 374 251 175 91 56 52
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 1561 1966 2826
Size: 1507 54 405 860
Fragments arranged by size:
1507 860 405 54
BsiEI CG_ry'CG
Cuts at: 0 1232 2826
Size: 1232 1594
BsiHKAI G_wGCw'C
Cuts at: 0 515 845 1385 1762 2318 2825 2826
Size: 515 330 540 377 556 507 1
Fragments arranged by size:
556 540 515 507 377 330 1
BsmI GAATG_Cn'
Cuts at: 0 221 2826
Size: 221 2605
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1231 1729 2826
Size: 1231 498 1097
Fragments arranged by size:
1231 1097 498
Bsp1286I G_dGCh'C
Cuts at: 0 515 845 1128 1385 1762 1815 2181 2318
Size: 515 330 283 257 377 53 366 137
Cuts at: 2318 2407 2576 2825 2826
Size: 89 169 249 1
Fragments arranged by size:
515 377 366 330 283 257 249 169
137 89 53 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1583 2826
Size: 1583 1243
BsrBI CCG'CTC
Cuts at: 0 1443 1797 2826
Size: 1443 354 1029
Fragments arranged by size:
1443 1029 354
BsrDI GCAATG_nn'
Cuts at: 0 1538 2480 2826
Size: 1538 942 346
Fragments arranged by size:
1538 942 346
BsrFI r'CCGG_y
Cuts at: 0 1875 1938 2445 2826
Size: 1875 63 507 381
Fragments arranged by size:
1875 507 381 63
BsrGI T'GTAC_A
Cuts at: 0 1079 2826
Size: 1079 1747
BssSI C'ACGA_G
Cuts at: 0 1384 2826
Size: 1384 1442
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1953 2826
Size: 1953 873
BstEII G'GTnAC_C
Cuts at: 0 1587 2826
Size: 1587 1239
BstXI CCAn_nnnn'nTGG
Cuts at: 0 885 1482 2666 2826
Size: 885 597 1184 160
Fragments arranged by size:
1184 885 597 160
BstYI r'GATC_y
Cuts at: 0 1253 2257 2826
Size: 1253 1004 569
Fragments arranged by size:
1253 1004 569
Bsu36I CC'TnA_GG
Cuts at: 0 2119 2624 2826
Size: 2119 505 202
Fragments arranged by size:
2119 505 202
BtgI C'CryG_G
Cuts at: 0 1226 1908 1925 2514 2826
Size: 1226 682 17 589 312
Fragments arranged by size:
1226 682 589 312 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 573 1706 2826
Size: 573 1133 1120
Fragments arranged by size:
1133 1120 573
BtsI GCAGTG_nn'
Cuts at: 0 440 642 912 2826
Size: 440 202 270 1914
Fragments arranged by size:
1914 440 270 202
ClaI AT'CG_AT
Cuts at: 0 1463 2826
Size: 1463 1363
DraI TTT'AAA
Cuts at: 0 717 2826
Size: 717 2109
DraIII CAC_nnn'GTG
Cuts at: 0 2084 2826
Size: 2084 742
DrdI GACnn_nn'nnGTC
Cuts at: 0 2149 2525 2826
Size: 2149 376 301
Fragments arranged by size:
2149 376 301
EaeI y'GGCC_r
Cuts at: 0 2196 2802 2826
Size: 2196 606 24
Fragments arranged by size:
2196 606 24
EarI CTCTTCn'nnn_
Cuts at: 0 50 84 343 958 2690 2826
Size: 50 34 259 615 1732 136
Fragments arranged by size:
1732 615 259 136 50 34
EcoICRI GAG'CTC
Cuts at: 0 1760 2823 2826
Size: 1760 1063 3
Fragments arranged by size:
1760 1063 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 595 619 1037 1124 1314 1360 1375
Size: 254 341 24 418 87 190 46 15
Cuts at: 1375 1642 1706 1749 1918 2377 2590 2826
Size: 267 64 43 169 459 213 236
Fragments arranged by size:
459 418 341 267 254 236 213 190
169 87 64 46 43 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1900 2539 2826
Size: 1900 639 287
Fragments arranged by size:
1900 639 287
EcoO109I rG'GnC_Cy
Cuts at: 0 102 1124 1125 1436 1818 1930 1978 2496
Size: 102 1022 1 311 382 112 48 518
Cuts at: 2496 2572 2620 2826
Size: 76 48 206
Fragments arranged by size:
1022 518 382 311 206 112 102 76
48 48 1
EcoRI G'AATT_C
Cuts at: 0 157 2826
Size: 157 2669
EcoRV GAT'ATC
Cuts at: 0 1043 2826
Size: 1043 1783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1053 2826
Size: 1053 1773
FspI TGC'GCA
Cuts at: 0 2224 2826
Size: 2224 602
HaeII r_GCGC'y
Cuts at: 0 498 2452 2826
Size: 498 1954 374
Fragments arranged by size:
1954 498 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 758 773 790 805 1274 1306
Size: 86 32 640 15 17 15 469 32
Cuts at: 1306 1333 1365 1387 1419 1970 2002 2247 2249
Size: 27 32 22 32 551 32 245 2
Cuts at: 2249 2279 2281 2300 2332 2826
Size: 30 2 19 32 494
Fragments arranged by size:
640 551 494 469 245 86 32 32
32 32 32 32 30 27 22 19
17 15 15 2 2
KasI G'GCGC_C
Cuts at: 0 494 2448 2826
Size: 494 1954 378
Fragments arranged by size:
1954 494 378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1034 2826
Size: 999 35 1792
Fragments arranged by size:
1792 999 35
MscI TGG'CCA
Cuts at: 0 2198 2804 2826
Size: 2198 606 22
Fragments arranged by size:
2198 606 22
MslI CAynn'nnrTG
Cuts at: 0 523 1858 2791 2826
Size: 523 1335 933 35
Fragments arranged by size:
1335 933 523 35
MspA1I CmG'CkG
Cuts at: 0 271 540 1809 2826
Size: 271 269 1269 1017
Fragments arranged by size:
1269 1017 271 269
NaeI GCC'GGC
Cuts at: 0 2447 2826
Size: 2447 379
NarI GG'CG_CC
Cuts at: 0 495 2449 2826
Size: 495 1954 377
Fragments arranged by size:
1954 495 377
NcoI C'CATG_G
Cuts at: 0 1925 2826
Size: 1925 901
NdeI CA'TA_TG
Cuts at: 0 1091 2826
Size: 1091 1735
NgoMIV G'CCGG_C
Cuts at: 0 2445 2826
Size: 2445 381
NspI r_CATG'y
Cuts at: 0 1917 2826
Size: 1917 909
PflMI CCAn_nnn'nTGG
Cuts at: 0 1821 2334 2826
Size: 1821 513 492
Fragments arranged by size:
1821 513 492
PfoI T'CCnGG_A
Cuts at: 0 544 572 2826
Size: 544 28 2254
Fragments arranged by size:
2254 544 28
PmlI CAC'GTG
Cuts at: 0 1581 2826
Size: 1581 1245
PpuMI rG'GwC_Cy
Cuts at: 0 102 1436 1930 1978 2826
Size: 102 1334 494 48 848
Fragments arranged by size:
1334 848 494 102 48
PspOMI G'GGCC_C
Cuts at: 0 1124 1811 2572 2826
Size: 1124 687 761 254
Fragments arranged by size:
1124 761 687 254
PstI C_TGCA'G
Cuts at: 0 214 276 435 491 1133 2571 2826
Size: 214 62 159 56 642 1438 255
Fragments arranged by size:
1438 642 255 214 159 62 56
PvuII CAG'CTG
Cuts at: 0 271 540 2826
Size: 271 269 2286
Fragments arranged by size:
2286 271 269
SacI G_AGCT'C
Cuts at: 0 1762 2825 2826
Size: 1762 1063 1
Fragments arranged by size:
1762 1063 1
SanDI GG'GwC_CC
Cuts at: 0 102 1930 2826
Size: 102 1828 896
Fragments arranged by size:
1828 896 102
SapI GCTCTTCn'nnn_
Cuts at: 0 2690 2826
Size: 2690 136
SbfI CC_TGCA'GG
Cuts at: 0 2571 2826
Size: 2571 255
SfcI C'TryA_G
Cuts at: 0 210 272 431 487 725 1129 2567 2826
Size: 210 62 159 56 238 404 1438 259
Fragments arranged by size:
1438 404 259 238 210 159 62 56
SfoI GGC'GCC
Cuts at: 0 496 2450 2826
Size: 496 1954 376
Fragments arranged by size:
1954 496 376
SmaI CCC'GGG
Cuts at: 0 2109 2826
Size: 2109 717
SmlI C'TyrA_G
Cuts at: 0 1249 1987 2266 2826
Size: 1249 738 279 560
Fragments arranged by size:
1249 738 560 279
SspI AAT'ATT
Cuts at: 0 419 2826
Size: 419 2407
StuI AGG'CCT
Cuts at: 0 1683 2826
Size: 1683 1143
StyI C'CwwG_G
Cuts at: 0 941 1925 2826
Size: 941 984 901
Fragments arranged by size:
984 941 901
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1113 2826
Size: 1113 1713
TatI w'GTAC_w
Cuts at: 0 729 1079 2753 2826
Size: 729 350 1674 73
Fragments arranged by size:
1674 729 350 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 299 331 2826
Size: 299 32 2495
Fragments arranged by size:
2495 299 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 826 1174 2826
Size: 826 348 1652
Fragments arranged by size:
1652 826 348
XhoI C'TCGA_G
Cuts at: 0 2266 2826
Size: 2266 560
XmaI C'CCGG_G
Cuts at: 0 2107 2826
Size: 2107 719
ZraI GAC'GTC
Cuts at: 0 789 2826
Size: 789 2037
Enzymes that do cut:
AarI AatII AccI AcuI AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SbfI SfcI SfoI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AleI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BmtI
BplI BsiWI BspEI BspHI BssHII BstBI BstZ17I CspCI
EagI EciI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XmnI