Restriction map for
pF1KSDA1956
(Linear) (Six-base) MAPSORT of: KIAA1956.seq Check: 7270 from: 1 to: 2028
KIAA1956.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 211 2028
Size: 211 1817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1878 2028
Size: 1878 150
AflIII A'CryG_T
Cuts at: 0 645 2028
Size: 645 1383
AleI CACnn'nnGTG
Cuts at: 0 248 332 1094 2028
Size: 248 84 762 934
Fragments arranged by size:
934 762 248 84
AlwNI CAG_nnn'CTG
Cuts at: 0 324 2028
Size: 324 1704
ApoI r'AATT_y
Cuts at: 0 1717 2028
Size: 1717 311
AvaI C'yCGr_G
Cuts at: 0 1841 2028
Size: 1841 187
BanI G'GyrC_C
Cuts at: 0 162 1147 2028
Size: 162 985 881
Fragments arranged by size:
985 881 162
BanII G_rGCy'C
Cuts at: 0 1651 2028
Size: 1651 377
BfrBI ATG'CAT
Cuts at: 0 623 2028
Size: 623 1405
Bme1580I G_kGCm'C
Cuts at: 0 10 1150 2028
Size: 10 1140 878
Fragments arranged by size:
1140 878 10
BmgBI CAC'GTC
Cuts at: 0 96 2028
Size: 96 1932
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 554 767 851 2028
Size: 120 434 213 84 1177
Fragments arranged by size:
1177 434 213 120 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 947 1031 1283 1367 1451 1535 1619
Size: 124 823 84 252 84 84 84 84
Cuts at: 1619 1710 1778 1946 2028
Size: 91 68 168 82
Fragments arranged by size:
823 252 168 124 91 84 84 84
84 84 82 68
Bpu10I CC'TnA_GC
Cuts at: 0 513 2028
Size: 513 1515
BsaAI yAC'GTr
Cuts at: 0 648 2028
Size: 648 1380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 172 481 2028
Size: 64 108 309 1547
Fragments arranged by size:
1547 309 108 64
BseYI C'CCAG_C
Cuts at: 0 508 695 2028
Size: 508 187 1333
Fragments arranged by size:
1333 508 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1722 1881 1965 2028
Size: 310 1412 159 84 63
Fragments arranged by size:
1412 310 159 84 63
BsiHKAI G_wGCw'C
Cuts at: 0 1651 2028
Size: 1651 377
BsmI GAATG_Cn'
Cuts at: 0 625 1780 2028
Size: 625 1155 248
Fragments arranged by size:
1155 625 248
Bsp1286I G_dGCh'C
Cuts at: 0 10 1150 1651 2028
Size: 10 1140 501 377
Fragments arranged by size:
1140 501 377 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 211 2028
Size: 211 1817
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 2028
Size: 324 1704
Bsu36I CC'TnA_GG
Cuts at: 0 1237 2028
Size: 1237 791
BtsI GCAGTG_nn'
Cuts at: 0 423 1679 1953 2028
Size: 423 1256 274 75
Fragments arranged by size:
1256 423 274 75
DraI TTT'AAA
Cuts at: 0 1821 2028
Size: 1821 207
EarI CTCTTCn'nnn_
Cuts at: 0 169 436 1656 1983 2028
Size: 169 267 1220 327 45
Fragments arranged by size:
1220 327 267 169 45
EcoICRI GAG'CTC
Cuts at: 0 1649 2028
Size: 1649 379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 947 1031 1283 1367 1451 1535 1619
Size: 124 823 84 252 84 84 84 84
Cuts at: 1619 1710 1778 1878 1946 2028
Size: 91 68 100 68 82
Fragments arranged by size:
823 252 124 100 91 84 84 84
84 84 82 68 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 218 2028
Size: 67 151 1810
Fragments arranged by size:
1810 151 67
FspI TGC'GCA
Cuts at: 0 1814 2028
Size: 1814 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 562 594 2028
Size: 457 32 73 32 1434
Fragments arranged by size:
1434 457 73 32 32
MslI CAynn'nnrTG
Cuts at: 0 248 332 1094 1446 2028
Size: 248 84 762 352 582
Fragments arranged by size:
762 582 352 248 84
MspA1I CmG'CkG
Cuts at: 0 611 2028
Size: 611 1417
NdeI CA'TA_TG
Cuts at: 0 1443 2028
Size: 1443 585
NsiI A_TGCA'T
Cuts at: 0 625 2028
Size: 625 1403
PasI CC'CwG_GG
Cuts at: 0 129 2028
Size: 129 1899
PspXI vC'TCGA_Gb
Cuts at: 0 1841 2028
Size: 1841 187
PstI C_TGCA'G
Cuts at: 0 530 1686 2028
Size: 530 1156 342
Fragments arranged by size:
1156 530 342
PvuII CAG'CTG
Cuts at: 0 611 2028
Size: 611 1417
SacI G_AGCT'C
Cuts at: 0 1651 2028
Size: 1651 377
SapI GCTCTTCn'nnn_
Cuts at: 0 169 1656 1983 2028
Size: 169 1487 327 45
Fragments arranged by size:
1487 327 169 45
SfcI C'TryA_G
Cuts at: 0 408 526 1682 2028
Size: 408 118 1156 346
Fragments arranged by size:
1156 408 346 118
SmlI C'TyrA_G
Cuts at: 0 1841 2028
Size: 1841 187
SpeI A'CTAG_T
Cuts at: 0 1093 2028
Size: 1093 935
StuI AGG'CCT
Cuts at: 0 1356 1608 1767 1935 2028
Size: 1356 252 159 168 93
Fragments arranged by size:
1356 252 168 159 93
StyI C'CwwG_G
Cuts at: 0 393 2028
Size: 393 1635
TatI w'GTAC_w
Cuts at: 0 1917 2028
Size: 1917 111
XbaI T'CTAG_A
Cuts at: 0 676 2028
Size: 676 1352
XhoI C'TCGA_G
Cuts at: 0 1841 2028
Size: 1841 187
XmnI GAAnn'nnTTC
Cuts at: 0 1720 1977 2028
Size: 1720 257 51
Fragments arranged by size:
1720 257 51
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApoI AvaI BanI
BanII BfrBI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BstAPI
Bsu36I BtsI DraI EarI EcoICRI Eco57MI EcoNI FspI
Hin4I MslI MspA1I NdeI NsiI PasI PspXI PstI
PvuII SacI SapI SfcI SmlI SpeI StuI StyI
TatI XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRI EcoRV FalI
FseI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI SwaI TaqII TstI Tth111I XcmI XmaI ZraI