Restriction map for
pF1KSDA1959
(Linear) (Six-base) MAPSORT of: KIAA1959.seq Check: 1434 from: 1 to: 1947
KIAA1959.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 707 1947
Size: 707 1240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1744 1947
Size: 1744 203
AflIII A'CryG_T
Cuts at: 0 633 1947
Size: 633 1314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1947
Size: 179 32 1736
Fragments arranged by size:
1736 179 32
AlwNI CAG_nnn'CTG
Cuts at: 0 458 586 965 1116 1767 1947
Size: 458 128 379 151 651 180
Fragments arranged by size:
651 458 379 180 151 128
AvaI C'yCGr_G
Cuts at: 0 260 743 829 1069 1947
Size: 260 483 86 240 878
Fragments arranged by size:
878 483 260 240 86
BanI G'GyrC_C
Cuts at: 0 97 1947
Size: 97 1850
BanII G_rGCy'C
Cuts at: 0 156 497 1070 1490 1947
Size: 156 341 573 420 457
Fragments arranged by size:
573 457 420 341 156
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 1947
Size: 542 1405
BbvCI CC'TCA_GC
Cuts at: 0 1117 1277 1947
Size: 1117 160 670
Fragments arranged by size:
1117 670 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1294 1328 1947
Size: 1294 34 619
Fragments arranged by size:
1294 619 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1535 1947
Size: 1535 412
BfrBI ATG'CAT
Cuts at: 0 1337 1947
Size: 1337 610
BglI GCCn_nnn'nGGC
Cuts at: 0 1136 1947
Size: 1136 811
BglII A'GATC_T
Cuts at: 0 338 1947
Size: 338 1609
BmgBI CAC'GTC
Cuts at: 0 126 896 1947
Size: 126 770 1051
Fragments arranged by size:
1051 770 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 732 1209 1919 1947
Size: 14 718 477 710 28
Fragments arranged by size:
718 710 477 28 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 838 1944 1947
Size: 511 327 1106 3
Fragments arranged by size:
1106 511 327 3
Bpu10I CC'TnA_GC
Cuts at: 0 297 1117 1277 1947
Size: 297 820 160 670
Fragments arranged by size:
820 670 297 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1947
Size: 538 1409
BsaI GGTCTCn'nnnn_
Cuts at: 0 1924 1947
Size: 1924 23
BsaHI Gr'CG_yC
Cuts at: 0 704 1947
Size: 704 1243
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1275 1305 1947
Size: 1275 30 642
Fragments arranged by size:
1275 642 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 121 1073 1947
Size: 61 60 952 874
Fragments arranged by size:
952 874 61 60
BseYI C'CCAG_C
Cuts at: 0 1519 1947
Size: 1519 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1415 1947
Size: 1415 532
BsiHKAI G_wGCw'C
Cuts at: 0 131 497 1528 1947
Size: 131 366 1031 419
Fragments arranged by size:
1031 419 366 131
BsmI GAATG_Cn'
Cuts at: 0 961 1947
Size: 961 986
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1069 1947
Size: 1069 878
Bsp1286I G_dGCh'C
Cuts at: 0 131 156 497 1070 1490 1528 1947
Size: 131 25 341 573 420 38 419
Fragments arranged by size:
573 420 419 341 131 38 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1542 1759 1947
Size: 1542 217 188
Fragments arranged by size:
1542 217 188
BsrBI CCG'CTC
Cuts at: 0 27 1947
Size: 27 1920
BsrFI r'CCGG_y
Cuts at: 0 289 907 1947
Size: 289 618 1040
Fragments arranged by size:
1040 618 289
BsrGI T'GTAC_A
Cuts at: 0 53 1947
Size: 53 1894
BstAPI GCAn_nnn'nTGC
Cuts at: 0 586 1947
Size: 586 1361
BstEII G'GTnAC_C
Cuts at: 0 232 1947
Size: 232 1715
BstYI r'GATC_y
Cuts at: 0 338 884 1475 1811 1875 1947
Size: 338 546 591 336 64 72
Fragments arranged by size:
591 546 338 336 72 64
BtgI C'CryG_G
Cuts at: 0 137 179 446 1899 1947
Size: 137 42 267 1453 48
Fragments arranged by size:
1453 267 137 48 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1164 1947
Size: 1164 783
ClaI AT'CG_AT
Cuts at: 0 1388 1947
Size: 1388 559
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1334 1369 1947
Size: 1334 35 578
Fragments arranged by size:
1334 578 35
EaeI y'GGCC_r
Cuts at: 0 12 1947
Size: 12 1935
EcoICRI GAG'CTC
Cuts at: 0 495 1947
Size: 495 1452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 838 1744 1944 1947
Size: 511 327 906 200 3
Fragments arranged by size:
906 511 327 200 3
EcoO109I rG'GnC_Cy
Cuts at: 0 549 1764 1947
Size: 549 1215 183
Fragments arranged by size:
1215 549 183
EcoRV GAT'ATC
Cuts at: 0 750 1947
Size: 750 1197
HaeII r_GCGC'y
Cuts at: 0 93 121 1947
Size: 93 28 1826
Fragments arranged by size:
1826 93 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 1068 1100 1803 1835 1947
Size: 574 32 462 32 703 32 112
Fragments arranged by size:
703 574 462 112 32 32 32
HincII GTy'rAC
Cuts at: 0 1125 1947
Size: 1125 822
HindIII A'AGCT_T
Cuts at: 0 307 1947
Size: 307 1640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 533 862 1034 1947
Size: 338 195 329 172 913
Fragments arranged by size:
913 338 329 195 172
MslI CAynn'nnrTG
Cuts at: 0 230 844 1324 1947
Size: 230 614 480 623
Fragments arranged by size:
623 614 480 230
MspA1I CmG'CkG
Cuts at: 0 483 1116 1281 1869 1947
Size: 483 633 165 588 78
Fragments arranged by size:
633 588 483 165 78
NcoI C'CATG_G
Cuts at: 0 137 1899 1947
Size: 137 1762 48
Fragments arranged by size:
1762 137 48
NsiI A_TGCA'T
Cuts at: 0 1339 1947
Size: 1339 608
NspI r_CATG'y
Cuts at: 0 209 610 637 1263 1341 1947
Size: 209 401 27 626 78 606
Fragments arranged by size:
626 606 401 209 78 27
PasI CC'CwG_GG
Cuts at: 0 426 1947
Size: 426 1521
PciI A'CATG_T
Cuts at: 0 633 1947
Size: 633 1314
PfoI T'CCnGG_A
Cuts at: 0 242 1947
Size: 242 1705
PpuMI rG'GwC_Cy
Cuts at: 0 549 1947
Size: 549 1398
PshAI GACnn'nnGTC
Cuts at: 0 448 1947
Size: 448 1499
PstI C_TGCA'G
Cuts at: 0 443 582 1566 1947
Size: 443 139 984 381
Fragments arranged by size:
984 443 381 139
PvuII CAG'CTG
Cuts at: 0 1869 1947
Size: 1869 78
SacI G_AGCT'C
Cuts at: 0 497 1947
Size: 497 1450
ScaI AGT'ACT
Cuts at: 0 1204 1947
Size: 1204 743
SfcI C'TryA_G
Cuts at: 0 439 578 1562 1590 1947
Size: 439 139 984 28 357
Fragments arranged by size:
984 439 357 139 28
SmaI CCC'GGG
Cuts at: 0 262 831 1947
Size: 262 569 1116
Fragments arranged by size:
1116 569 262
SmlI C'TyrA_G
Cuts at: 0 553 1947
Size: 553 1394
SpeI A'CTAG_T
Cuts at: 0 1350 1947
Size: 1350 597
SphI G_CATG'C
Cuts at: 0 1341 1947
Size: 1341 606
StyI C'CwwG_G
Cuts at: 0 137 170 944 1784 1899 1947
Size: 137 33 774 840 115 48
Fragments arranged by size:
840 774 137 115 48 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 714 1345 1947
Size: 714 631 602
Fragments arranged by size:
714 631 602
TatI w'GTAC_w
Cuts at: 0 53 1202 1947
Size: 53 1149 745
Fragments arranged by size:
1149 745 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1717 1749 1947
Size: 1717 32 198
Fragments arranged by size:
1717 198 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1906 1947
Size: 1906 41
XmaI C'CCGG_G
Cuts at: 0 260 829 1947
Size: 260 569 1118
Fragments arranged by size:
1118 569 260
ZraI GAC'GTC
Cuts at: 0 705 1947
Size: 705 1242
Enzymes that do cut:
AatII AcuI AflIII AloI AlwNI AvaI BanI BanII
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BstAPI BstEII BstYI BtgI BtgZI ClaI
CspCI EaeI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I
HincII HindIII MmeI MslI MspA1I NcoI NsiI NspI
PasI PciI PfoI PpuMI PshAI PstI PvuII SacI
ScaI SfcI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BclI BlpI Bme1580I BmtI
BplI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI
BsrDI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtsI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI