Restriction map for
pF1KSDA1963
(Linear) (Six-base) MAPSORT of: KIAA1963.seq Check: 4193 from: 1 to: 969
KIAA1963.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 425 969
Size: 425 544
AflIII A'CryG_T
Cuts at: 0 308 969
Size: 308 661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 106 433 891 969
Size: 106 327 458 78
Fragments arranged by size:
458 327 106 78
AvaI C'yCGr_G
Cuts at: 0 451 969
Size: 451 518
BamHI G'GATC_C
Cuts at: 0 44 338 796 969
Size: 44 294 458 173
Fragments arranged by size:
458 294 173 44
BanI G'GyrC_C
Cuts at: 0 145 509 969
Size: 145 364 460
Fragments arranged by size:
460 364 145
BanII G_rGCy'C
Cuts at: 0 581 969
Size: 581 388
BbeI G_GCGC'C
Cuts at: 0 149 969
Size: 149 820
BbsI GAAGACnn'nnnn_
Cuts at: 0 742 969
Size: 742 227
BglI GCCn_nnn'nGGC
Cuts at: 0 143 969
Size: 143 826
Bme1580I G_kGCm'C
Cuts at: 0 95 428 969
Size: 95 333 541
Fragments arranged by size:
541 333 95
BmgBI CAC'GTC
Cuts at: 0 72 969
Size: 72 897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 720 969
Size: 595 125 249
Fragments arranged by size:
595 249 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 615 969
Size: 615 354
BsaAI yAC'GTr
Cuts at: 0 423 969
Size: 423 546
BsaHI Gr'CG_yC
Cuts at: 0 146 969
Size: 146 823
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 143 173 969
Size: 143 30 796
Fragments arranged by size:
796 143 30
BseYI C'CCAG_C
Cuts at: 0 406 800 969
Size: 406 394 169
Fragments arranged by size:
406 394 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 316 403 861 969
Size: 298 18 87 458 108
Fragments arranged by size:
458 298 108 87 18
BsiEI CG_ry'CG
Cuts at: 0 211 969
Size: 211 758
BsiHKAI G_wGCw'C
Cuts at: 0 542 581 969
Size: 542 39 388
Fragments arranged by size:
542 388 39
Bsp1286I G_dGCh'C
Cuts at: 0 95 428 542 581 969
Size: 95 333 114 39 388
Fragments arranged by size:
388 333 114 95 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 425 969
Size: 425 544
BsrBI CCG'CTC
Cuts at: 0 99 159 969
Size: 99 60 810
Fragments arranged by size:
810 99 60
BsrFI r'CCGG_y
Cuts at: 0 694 969
Size: 694 275
BssHII G'CGCG_C
Cuts at: 0 361 462 522 524 969
Size: 361 101 60 2 445
Fragments arranged by size:
445 361 101 60 2
BssSI C'ACGA_G
Cuts at: 0 889 969
Size: 889 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 739 969
Size: 739 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 969
Size: 729 240
BstYI r'GATC_y
Cuts at: 0 44 338 796 969
Size: 44 294 458 173
Fragments arranged by size:
458 294 173 44
BtgI C'CryG_G
Cuts at: 0 138 433 969
Size: 138 295 536
Fragments arranged by size:
536 295 138
EaeI y'GGCC_r
Cuts at: 0 208 302 969
Size: 208 94 667
Fragments arranged by size:
667 208 94
EagI C'GGCC_G
Cuts at: 0 208 969
Size: 208 761
EarI CTCTTCn'nnn_
Cuts at: 0 547 586 969
Size: 547 39 383
Fragments arranged by size:
547 383 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 304 969
Size: 304 665
EcoICRI GAG'CTC
Cuts at: 0 579 969
Size: 579 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 720 969
Size: 595 125 249
Fragments arranged by size:
595 249 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 969
Size: 346 623
EcoO109I rG'GnC_Cy
Cuts at: 0 185 969
Size: 185 784
HaeII r_GCGC'y
Cuts at: 0 149 441 648 969
Size: 149 292 207 321
Fragments arranged by size:
321 292 207 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 969
Size: 143 32 794
Fragments arranged by size:
794 143 32
KasI G'GCGC_C
Cuts at: 0 145 969
Size: 145 824
MscI TGG'CCA
Cuts at: 0 304 969
Size: 304 665
MslI CAynn'nnrTG
Cuts at: 0 61 727 969
Size: 61 666 242
Fragments arranged by size:
666 242 61
MspA1I CmG'CkG
Cuts at: 0 140 234 330 435 660 969
Size: 140 94 96 105 225 309
Fragments arranged by size:
309 225 140 105 96 94
NarI GG'CG_CC
Cuts at: 0 146 969
Size: 146 823
PfoI T'CCnGG_A
Cuts at: 0 584 969
Size: 584 385
PmlI CAC'GTG
Cuts at: 0 423 969
Size: 423 546
PpuMI rG'GwC_Cy
Cuts at: 0 185 969
Size: 185 784
PvuII CAG'CTG
Cuts at: 0 234 330 969
Size: 234 96 639
Fragments arranged by size:
639 234 96
SacI G_AGCT'C
Cuts at: 0 581 969
Size: 581 388
SacII CC_GC'GG
Cuts at: 0 141 436 969
Size: 141 295 533
Fragments arranged by size:
533 295 141
SanDI GG'GwC_CC
Cuts at: 0 185 969
Size: 185 784
SapI GCTCTTCn'nnn_
Cuts at: 0 547 586 969
Size: 547 39 383
Fragments arranged by size:
547 383 39
SfcI C'TryA_G
Cuts at: 0 264 567 969
Size: 264 303 402
Fragments arranged by size:
402 303 264
SfoI GGC'GCC
Cuts at: 0 147 969
Size: 147 822
SmaI CCC'GGG
Cuts at: 0 453 969
Size: 453 516
SrfI GCCC'GGGC
Cuts at: 0 453 969
Size: 453 516
StuI AGG'CCT
Cuts at: 0 714 969
Size: 714 255
XmaI C'CCGG_G
Cuts at: 0 451 969
Size: 451 518
Enzymes that do cut:
AarI AflIII AlfI AvaI BamHI BanI BanII BbeI
BbsI BglI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NarI PfoI PmlI
PpuMI PvuII SacI SacII SanDI SapI SfcI SfoI
SmaI SrfI StuI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI