Restriction map for
pF1KA1973
(Linear) (Six-base) MAPSORT of: KIAA1973.seq Check: 7368 from: 1 to: 3345
KIAA1973.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 2864 3345
Size: 2864 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 2135 2426 2515 3345
Size: 1268 867 291 89 830
Fragments arranged by size:
1268 867 830 291 89
AflIII A'CryG_T
Cuts at: 0 2994 3345
Size: 2994 351
AhdI GACnn_n'nnGTC
Cuts at: 0 1103 3345
Size: 1103 2242
AleI CACnn'nnGTG
Cuts at: 0 973 3345
Size: 973 2372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 2078 3345
Size: 1762 316 1267
Fragments arranged by size:
1762 1267 316
AlwNI CAG_nnn'CTG
Cuts at: 0 38 3276 3345
Size: 38 3238 69
Fragments arranged by size:
3238 69 38
ApaLI G'TGCA_C
Cuts at: 0 157 3345
Size: 157 3188
ApoI r'AATT_y
Cuts at: 0 729 1711 3345
Size: 729 982 1634
Fragments arranged by size:
1634 982 729
AvaI C'yCGr_G
Cuts at: 0 337 1976 3244 3345
Size: 337 1639 1268 101
Fragments arranged by size:
1639 1268 337 101
BanI G'GyrC_C
Cuts at: 0 81 267 346 3345
Size: 81 186 79 2999
Fragments arranged by size:
2999 186 81 79
BanII G_rGCy'C
Cuts at: 0 228 328 626 1121 2801 3164 3245 3345
Size: 228 100 298 495 1680 363 81 100
Fragments arranged by size:
1680 495 363 298 228 100 100 81
BbeI G_GCGC'C
Cuts at: 0 271 350 3345
Size: 271 79 2995
Fragments arranged by size:
2995 271 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 1569 1772 2061 2130 3345
Size: 821 748 203 289 69 1215
Fragments arranged by size:
1215 821 748 289 203 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 1012 2608 3345
Size: 1012 1596 737
Fragments arranged by size:
1596 1012 737
BclI T'GATC_A
Cuts at: 0 659 3345
Size: 659 2686
BfrBI ATG'CAT
Cuts at: 0 2439 3345
Size: 2439 906
BglI GCCn_nnn'nGGC
Cuts at: 0 463 1116 1616 1879 3345
Size: 463 653 500 263 1466
Fragments arranged by size:
1466 653 500 463 263
BglII A'GATC_T
Cuts at: 0 2315 3345
Size: 2315 1030
BlpI GC'TnA_GC
Cuts at: 0 28 3345
Size: 28 3317
Bme1580I G_kGCm'C
Cuts at: 0 86 137 161 1612 1706 1835 3345
Size: 86 51 24 1451 94 129 1510
Fragments arranged by size:
1510 1451 129 94 86 51 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1023 1850 3345
Size: 1023 827 1495
Fragments arranged by size:
1495 1023 827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3328 3345
Size: 3328 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 942 964 1563 3345
Size: 106 836 22 599 1782
Fragments arranged by size:
1782 836 599 106 22
BsaAI yAC'GTr
Cuts at: 0 1164 3345
Size: 1164 2181
BsaBI GATnn'nnATC
Cuts at: 0 457 664 3345
Size: 457 207 2681
Fragments arranged by size:
2681 457 207
BsaHI Gr'CG_yC
Cuts at: 0 268 347 377 2519 3345
Size: 268 79 30 2142 826
Fragments arranged by size:
2142 826 268 79 30
BsaWI w'CCGG_w
Cuts at: 0 204 3345
Size: 204 3141
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 349 379 517 547 889 919 1974
Size: 27 322 30 138 30 342 30 1055
Cuts at: 1974 2004 3345
Size: 30 1341
Fragments arranged by size:
1341 1055 342 322 138 30 30 30
30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 81 84 489 829 3325 3345
Size: 18 63 3 405 340 2496 20
Fragments arranged by size:
2496 405 340 63 20 18 3
BseYI C'CCAG_C
Cuts at: 0 85 1065 1240 2034 3111 3345
Size: 85 980 175 794 1077 234
Fragments arranged by size:
1077 980 794 234 175 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1892 2038 3345
Size: 631 1261 146 1307
Fragments arranged by size:
1307 1261 631 146
BsiHKAI G_wGCw'C
Cuts at: 0 161 301 626 1160 2858 3164 3245 3345
Size: 161 140 325 534 1698 306 81 100
Fragments arranged by size:
1698 534 325 306 161 140 100 81
Bsp1286I G_dGCh'C
Cuts at: 0 86 137 161 228 301 328 626 1121
Size: 86 51 24 67 73 27 298 495
Cuts at: 1121 1160 1612 1706 1835 2801 2858 3164 3245
Size: 39 452 94 129 966 57 306 81
Cuts at: 3245 3345
Size: 100
Fragments arranged by size:
966 495 452 306 298 129 100 94
86 81 73 67 57 51 39 27
24
BspEI T'CCGG_A
Cuts at: 0 204 3345
Size: 204 3141
BspHI T'CATG_A
Cuts at: 0 3060 3345
Size: 3060 285
BsrDI GCAATG_nn'
Cuts at: 0 1121 3345
Size: 1121 2224
BssSI C'ACGA_G
Cuts at: 0 676 3345
Size: 676 2669
BstBI TT'CG_AA
Cuts at: 0 1016 3345
Size: 1016 2329
BstEII G'GTnAC_C
Cuts at: 0 1551 3345
Size: 1551 1794
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1956 2021 2216 2997 3345
Size: 1956 65 195 781 348
Fragments arranged by size:
1956 781 348 195 65
BstYI r'GATC_y
Cuts at: 0 116 935 2315 3122 3345
Size: 116 819 1380 807 223
Fragments arranged by size:
1380 819 807 223 116
BstZ17I GTA'TAC
Cuts at: 0 2865 3345
Size: 2865 480
Bsu36I CC'TnA_GG
Cuts at: 0 1113 3345
Size: 1113 2232
BtgI C'CryG_G
Cuts at: 0 370 392 514 560 685 1415 3345
Size: 370 22 122 46 125 730 1930
Fragments arranged by size:
1930 730 370 125 122 46 22
BtsI GCAGTG_nn'
Cuts at: 0 528 2060 2406 3345
Size: 528 1532 346 939
Fragments arranged by size:
1532 939 528 346
DraIII CAC_nnn'GTG
Cuts at: 0 141 3345
Size: 141 3204
DrdI GACnn_nn'nnGTC
Cuts at: 0 258 3339 3345
Size: 258 3081 6
Fragments arranged by size:
3081 258 6
EaeI y'GGCC_r
Cuts at: 0 74 191 309 367 455 1486 1617 2011
Size: 74 117 118 58 88 1031 131 394
Cuts at: 2011 2195 3345
Size: 184 1150
Fragments arranged by size:
1150 1031 394 184 131 118 117 88
74 58
EarI CTCTTCn'nnn_
Cuts at: 0 1656 2086 2318 3150 3345
Size: 1656 430 232 832 195
Fragments arranged by size:
1656 832 430 232 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 370 1023 3345
Size: 370 653 2322
Fragments arranged by size:
2322 653 370
EcoICRI GAG'CTC
Cuts at: 0 624 3162 3243 3345
Size: 624 2538 81 102
Fragments arranged by size:
2538 624 102 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 2135 2426 2515 3328 3345
Size: 1268 867 291 89 813 17
Fragments arranged by size:
1268 867 813 291 89 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2366 3345
Size: 2366 979
EcoO109I rG'GnC_Cy
Cuts at: 0 360 915 1973 3019 3345
Size: 360 555 1058 1046 326
Fragments arranged by size:
1058 1046 555 360 326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1557 3345
Size: 1557 1788
FspI TGC'GCA
Cuts at: 0 63 3345
Size: 63 3282
HaeII r_GCGC'y
Cuts at: 0 271 350 586 3345
Size: 271 79 236 2759
Fragments arranged by size:
2759 271 236 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 684 716 1089 1121 1551 1583
Size: 517 32 135 32 373 32 430 32
Cuts at: 1583 3345
Size: 1762
Fragments arranged by size:
1762 517 430 373 135 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 267 346 3345
Size: 267 79 2999
Fragments arranged by size:
2999 267 79
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1242 1545 2197 3310 3345
Size: 1242 303 652 1113 35
Fragments arranged by size:
1242 1113 652 303 35
MscI TGG'CCA
Cuts at: 0 369 457 1488 1619 2013 2197 3345
Size: 369 88 1031 131 394 184 1148
Fragments arranged by size:
1148 1031 394 369 184 131 88
MslI CAynn'nnrTG
Cuts at: 0 655 973 1261 1966 3345
Size: 655 318 288 705 1379
Fragments arranged by size:
1379 705 655 318 288
MspA1I CmG'CkG
Cuts at: 0 1448 3111 3282 3345
Size: 1448 1663 171 63
Fragments arranged by size:
1663 1448 171 63
NarI GG'CG_CC
Cuts at: 0 268 347 3345
Size: 268 79 2998
Fragments arranged by size:
2998 268 79
NcoI C'CATG_G
Cuts at: 0 514 1415 3345
Size: 514 901 1930
Fragments arranged by size:
1930 901 514
NsiI A_TGCA'T
Cuts at: 0 2441 3345
Size: 2441 904
PflMI CCAn_nnn'nTGG
Cuts at: 0 561 1083 3345
Size: 561 522 2262
Fragments arranged by size:
2262 561 522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1217 1249 3345
Size: 1217 32 2096
Fragments arranged by size:
2096 1217 32
PpuMI rG'GwC_Cy
Cuts at: 0 915 1973 3019 3345
Size: 915 1058 1046 326
Fragments arranged by size:
1058 1046 915 326
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 769 801 3345
Size: 769 32 2544
Fragments arranged by size:
2544 769 32
PstI C_TGCA'G
Cuts at: 0 3281 3345
Size: 3281 64
PvuII CAG'CTG
Cuts at: 0 1448 3111 3282 3345
Size: 1448 1663 171 63
Fragments arranged by size:
1663 1448 171 63
SacI G_AGCT'C
Cuts at: 0 626 3164 3245 3345
Size: 626 2538 81 100
Fragments arranged by size:
2538 626 100 81
SanDI GG'GwC_CC
Cuts at: 0 3019 3345
Size: 3019 326
SapI GCTCTTCn'nnn_
Cuts at: 0 2318 3345
Size: 2318 1027
SfcI C'TryA_G
Cuts at: 0 931 1196 3277 3345
Size: 931 265 2081 68
Fragments arranged by size:
2081 931 265 68
SfoI GGC'GCC
Cuts at: 0 269 348 3345
Size: 269 79 2997
Fragments arranged by size:
2997 269 79
SmaI CCC'GGG
Cuts at: 0 339 3345
Size: 339 3006
SmlI C'TyrA_G
Cuts at: 0 121 921 943 1542 3244 3345
Size: 121 800 22 599 1702 101
Fragments arranged by size:
1702 800 599 121 101 22
SnaBI TAC'GTA
Cuts at: 0 1164 3345
Size: 1164 2181
SspI AAT'ATT
Cuts at: 0 1294 3345
Size: 1294 2051
StuI AGG'CCT
Cuts at: 0 470 1331 3345
Size: 470 861 2014
Fragments arranged by size:
2014 861 470
StyI C'CwwG_G
Cuts at: 0 514 870 1415 2114 2367 3148 3345
Size: 514 356 545 699 253 781 197
Fragments arranged by size:
781 699 545 514 356 253 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 981 1050 1840 2649 3345
Size: 925 56 69 790 809 696
Fragments arranged by size:
925 809 790 696 69 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 3345
Size: 349 32 2964
Fragments arranged by size:
2964 349 32
Tth111I GACn'n_nGTC
Cuts at: 0 631 3023 3345
Size: 631 2392 322
Fragments arranged by size:
2392 631 322
XbaI T'CTAG_A
Cuts at: 0 1629 3345
Size: 1629 1716
XhoI C'TCGA_G
Cuts at: 0 3244 3345
Size: 3244 101
XmaI C'CCGG_G
Cuts at: 0 337 3345
Size: 337 3008
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BpmI BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspHI BsrDI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NcoI NsiI PflMI PpiI PpuMI PsrI PstI
PvuII SacI SanDI SapI SfcI SfoI SmaI SmlI
SnaBI SspI StuI StyI TaqII TstI Tth111I XbaI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BmgBI BmtI BplI Bpu10I BsaI BsiEI BsiWI
BsmI BsmBI BspMI BsrBI BsrFI BsrGI BssHII BstAPI
BtgZI ClaI CspCI DraI EagI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SpeI SphI SrfI SwaI TatI XcmI
XmnI ZraI