Restriction map for
pF1KA1975
(Linear) (Six-base) MAPSORT of: KIAA1975.seq Check: 5077 from: 1 to: 1650
KIAA1975.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1370 1650
Size: 1370 280
AatII G_ACGT'C
Cuts at: 0 1541 1650
Size: 1541 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 657 1650
Size: 427 230 993
Fragments arranged by size:
993 427 230
AfeI AGC'GCT
Cuts at: 0 1571 1650
Size: 1571 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1630 1650
Size: 1630 20
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1274 1306 1650
Size: 1274 32 344
Fragments arranged by size:
1274 344 32
AlwNI CAG_nnn'CTG
Cuts at: 0 918 1220 1362 1518 1650
Size: 918 302 142 156 132
Fragments arranged by size:
918 302 156 142 132
ApaLI G'TGCA_C
Cuts at: 0 112 1650
Size: 112 1538
AvaI C'yCGr_G
Cuts at: 0 1547 1650
Size: 1547 103
BamHI G'GATC_C
Cuts at: 0 1289 1650
Size: 1289 361
BanI G'GyrC_C
Cuts at: 0 1120 1650
Size: 1120 530
BanII G_rGCy'C
Cuts at: 0 1175 1650
Size: 1175 475
BbsI GAAGACnn'nnnn_
Cuts at: 0 415 1650
Size: 415 1235
BbvCI CC'TCA_GC
Cuts at: 0 803 1650
Size: 803 847
BglI GCCn_nnn'nGGC
Cuts at: 0 565 1588 1650
Size: 565 1023 62
Fragments arranged by size:
1023 565 62
BglII A'GATC_T
Cuts at: 0 61 1650
Size: 61 1589
Bme1580I G_kGCm'C
Cuts at: 0 116 1650
Size: 116 1534
BmrI ACTGGGnnnn_n'
Cuts at: 0 674 1650
Size: 674 976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 567 672 1168 1578 1650
Size: 343 224 105 496 410 72
Fragments arranged by size:
496 410 343 224 105 72
Bpu10I CC'TnA_GC
Cuts at: 0 471 803 1174 1650
Size: 471 332 371 476
Fragments arranged by size:
476 471 371 332
BsaI GGTCTCn'nnnn_
Cuts at: 0 1033 1435 1650
Size: 1033 402 215
Fragments arranged by size:
1033 402 215
BsaHI Gr'CG_yC
Cuts at: 0 1538 1650
Size: 1538 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 1650
Size: 145 30 1475
Fragments arranged by size:
1475 145 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 153 892 1444 1650
Size: 153 739 552 206
Fragments arranged by size:
739 552 206 153
BseYI C'CCAG_C
Cuts at: 0 181 654 959 1650
Size: 181 473 305 691
Fragments arranged by size:
691 473 305 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1320 1449 1650
Size: 1320 129 201
Fragments arranged by size:
1320 201 129
BsiHKAI G_wGCw'C
Cuts at: 0 116 455 1175 1650
Size: 116 339 720 475
Fragments arranged by size:
720 475 339 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1451 1650
Size: 1451 199
Bsp1286I G_dGCh'C
Cuts at: 0 116 455 1175 1650
Size: 116 339 720 475
Fragments arranged by size:
720 475 339 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 1370 1397 1650
Size: 722 648 27 253
Fragments arranged by size:
722 648 253 27
BsrBI CCG'CTC
Cuts at: 0 350 882 1650
Size: 350 532 768
Fragments arranged by size:
768 532 350
BsrDI GCAATG_nn'
Cuts at: 0 603 1206 1650
Size: 603 603 444
Fragments arranged by size:
603 603 444
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1365 1650
Size: 1365 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 545 615 1650
Size: 545 70 1035
Fragments arranged by size:
1035 545 70
BstYI r'GATC_y
Cuts at: 0 61 914 1289 1650
Size: 61 853 375 361
Fragments arranged by size:
853 375 361 61
BtgI C'CryG_G
Cuts at: 0 257 983 1557 1650
Size: 257 726 574 93
Fragments arranged by size:
726 574 257 93
BtsI GCAGTG_nn'
Cuts at: 0 221 797 1023 1650
Size: 221 576 226 627
Fragments arranged by size:
627 576 226 221
DraI TTT'AAA
Cuts at: 0 149 861 1650
Size: 149 712 789
Fragments arranged by size:
789 712 149
DraIII CAC_nnn'GTG
Cuts at: 0 437 1029 1650
Size: 437 592 621
Fragments arranged by size:
621 592 437
EaeI y'GGCC_r
Cuts at: 0 547 843 920 1162 1650
Size: 547 296 77 242 488
Fragments arranged by size:
547 488 296 242 77
EarI CTCTTCn'nnn_
Cuts at: 0 1268 1650
Size: 1268 382
EcoICRI GAG'CTC
Cuts at: 0 1173 1650
Size: 1173 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 427 567 657 672 1168 1578 1650
Size: 343 84 140 90 15 496 410 72
Fragments arranged by size:
496 410 343 140 90 84 72 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 607 1650
Size: 607 1043
EcoO109I rG'GnC_Cy
Cuts at: 0 198 1386 1650
Size: 198 1188 264
Fragments arranged by size:
1188 264 198
EcoRV GAT'ATC
Cuts at: 0 648 1650
Size: 648 1002
FspI TGC'GCA
Cuts at: 0 573 1650
Size: 573 1077
HaeII r_GCGC'y
Cuts at: 0 1474 1573 1650
Size: 1474 99 77
Fragments arranged by size:
1474 99 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 775 807 1032 1064 1650
Size: 18 32 725 32 225 32 586
Fragments arranged by size:
725 586 225 32 32 32 18
HincII GTy'rAC
Cuts at: 0 1265 1650
Size: 1265 385
HindIII A'AGCT_T
Cuts at: 0 468 1650
Size: 468 1182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1650
Size: 293 1357
MscI TGG'CCA
Cuts at: 0 549 845 922 1164 1650
Size: 549 296 77 242 486
Fragments arranged by size:
549 486 296 242 77
MslI CAynn'nnrTG
Cuts at: 0 1612 1650
Size: 1612 38
MspA1I CmG'CkG
Cuts at: 0 963 1378 1650
Size: 963 415 272
Fragments arranged by size:
963 415 272
NcoI C'CATG_G
Cuts at: 0 257 983 1650
Size: 257 726 667
Fragments arranged by size:
726 667 257
NdeI CA'TA_TG
Cuts at: 0 629 667 1650
Size: 629 38 983
Fragments arranged by size:
983 629 38
NspI r_CATG'y
Cuts at: 0 381 1011 1650
Size: 381 630 639
Fragments arranged by size:
639 630 381
PasI CC'CwG_GG
Cuts at: 0 1350 1650
Size: 1350 300
PflMI CCAn_nnn'nTGG
Cuts at: 0 263 918 1029 1650
Size: 263 655 111 621
Fragments arranged by size:
655 621 263 111
PpuMI rG'GwC_Cy
Cuts at: 0 198 1386 1650
Size: 198 1188 264
Fragments arranged by size:
1188 264 198
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1080 1112 1650
Size: 1080 32 538
Fragments arranged by size:
1080 538 32
PstI C_TGCA'G
Cuts at: 0 932 995 1625 1650
Size: 932 63 630 25
Fragments arranged by size:
932 630 63 25
PvuII CAG'CTG
Cuts at: 0 963 1650
Size: 963 687
SacI G_AGCT'C
Cuts at: 0 1175 1650
Size: 1175 475
SfcI C'TryA_G
Cuts at: 0 928 991 1621 1650
Size: 928 63 630 29
Fragments arranged by size:
928 630 63 29
SphI G_CATG'C
Cuts at: 0 381 1650
Size: 381 1269
SspI AAT'ATT
Cuts at: 0 492 1650
Size: 492 1158
StuI AGG'CCT
Cuts at: 0 1591 1650
Size: 1591 59
StyI C'CwwG_G
Cuts at: 0 20 257 608 983 1650
Size: 20 237 351 375 667
Fragments arranged by size:
667 375 351 237 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 591 646 1650
Size: 249 342 55 1004
Fragments arranged by size:
1004 342 249 55
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1009 1068 1114 1407 1650
Size: 1009 59 46 293 243
Fragments arranged by size:
1009 293 243 59 46
ZraI GAC'GTC
Cuts at: 0 1539 1650
Size: 1539 111
Enzymes that do cut:
AarI AatII AcuI AfeI AlfI AloI AlwNI ApaLI
AvaI BamHI BanI BanII BbsI BbvCI BglI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BstAPI BstXI BstYI BtgI BtsI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI
NdeI NspI PasI PflMI PpuMI PsrI PstI PvuII
SacI SfcI SphI SspI StuI StyI TaqII XcmI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DrdI EagI EciI EcoRI
FalI FseI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI