Restriction map for
pF1KSDA1993
(Linear) (Six-base) MAPSORT of: KIAA1993.seq Check: 5241 from: 1 to: 1512
KIAA1993.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1007 1512
Size: 1007 505
Acc65I G'GTAC_C
Cuts at: 0 779 1106 1333 1512
Size: 779 327 227 179
Fragments arranged by size:
779 327 227 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 322 455 1397 1512
Size: 319 3 133 942 115
Fragments arranged by size:
942 319 133 115 3
AleI CACnn'nnGTG
Cuts at: 0 709 1512
Size: 709 803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 1512
Size: 595 917
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1498 1512
Size: 1020 478 14
Fragments arranged by size:
1020 478 14
ApaLI G'TGCA_C
Cuts at: 0 363 1512
Size: 363 1149
ApoI r'AATT_y
Cuts at: 0 1484 1512
Size: 1484 28
AvaI C'yCGr_G
Cuts at: 0 46 445 1060 1079 1402 1512
Size: 46 399 615 19 323 110
Fragments arranged by size:
615 399 323 110 46 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 878 911 1243 1276 1512
Size: 878 33 332 33 236
Fragments arranged by size:
878 332 236 33 33
BanI G'GyrC_C
Cuts at: 0 779 1106 1333 1512
Size: 779 327 227 179
Fragments arranged by size:
779 327 227 179
BanII G_rGCy'C
Cuts at: 0 147 832 1033 1512
Size: 147 685 201 479
Fragments arranged by size:
685 479 201 147
BglII A'GATC_T
Cuts at: 0 497 1304 1512
Size: 497 807 208
Fragments arranged by size:
807 497 208
BlpI GC'TnA_GC
Cuts at: 0 946 1512
Size: 946 566
Bme1580I G_kGCm'C
Cuts at: 0 47 367 608 972 1512
Size: 47 320 241 364 540
Fragments arranged by size:
540 364 320 241 47
BmrI ACTGGGnnnn_n'
Cuts at: 0 484 1512
Size: 484 1028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 1512
Size: 624 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1276 1512
Size: 832 444 236
Fragments arranged by size:
832 444 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 656 671 1512
Size: 656 15 841
Fragments arranged by size:
841 656 15
BsaAI yAC'GTr
Cuts at: 0 1422 1512
Size: 1422 90
BsaWI w'CCGG_w
Cuts at: 0 551 1512
Size: 551 961
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 745 775 844 874 1512
Size: 745 30 69 30 638
Fragments arranged by size:
745 638 69 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 494 613 750 974 1101 1512
Size: 494 119 137 224 127 411
Fragments arranged by size:
494 411 224 137 127 119
BseYI C'CCAG_C
Cuts at: 0 568 742 1512
Size: 568 174 770
Fragments arranged by size:
770 568 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1045 1512
Size: 985 60 467
Fragments arranged by size:
985 467 60
BsiEI CG_ry'CG
Cuts at: 0 738 1512
Size: 738 774
BsiHKAI G_wGCw'C
Cuts at: 0 367 848 1512
Size: 367 481 664
Fragments arranged by size:
664 481 367
BsmBI CGTCTCn'nnnn_
Cuts at: 0 553 1512
Size: 553 959
Bsp1286I G_dGCh'C
Cuts at: 0 47 147 367 608 832 848 972 1033
Size: 47 100 220 241 224 16 124 61
Cuts at: 1033 1512
Size: 479
Fragments arranged by size:
479 241 224 220 124 100 61 47
16
BspEI T'CCGG_A
Cuts at: 0 551 1512
Size: 551 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1007 1019 1512
Size: 1007 12 493
Fragments arranged by size:
1007 493 12
BsrFI r'CCGG_y
Cuts at: 0 737 1512
Size: 737 775
BsrGI T'GTAC_A
Cuts at: 0 117 1512
Size: 117 1395
BstAPI GCAn_nnn'nTGC
Cuts at: 0 160 1512
Size: 160 1352
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1512
Size: 492 1020
BstYI r'GATC_y
Cuts at: 0 371 497 1304 1512
Size: 371 126 807 208
Fragments arranged by size:
807 371 208 126
BtgI C'CryG_G
Cuts at: 0 615 1512
Size: 615 897
BtsI GCAGTG_nn'
Cuts at: 0 352 549 630 824 1512
Size: 352 197 81 194 688
Fragments arranged by size:
688 352 197 194 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1206 1241 1512
Size: 1206 35 271
Fragments arranged by size:
1206 271 35
DraIII CAC_nnn'GTG
Cuts at: 0 1494 1512
Size: 1494 18
EarI CTCTTCn'nnn_
Cuts at: 0 431 1512
Size: 431 1081
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 1512
Size: 76 1436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 322 455 832 1276 1397 1512
Size: 319 3 133 377 444 121 115
Fragments arranged by size:
444 377 319 133 121 115 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1024 1512
Size: 1024 488
EcoO109I rG'GnC_Cy
Cuts at: 0 1017 1512
Size: 1017 495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 1186 1512
Size: 175 1011 326
Fragments arranged by size:
1011 326 175
FspI TGC'GCA
Cuts at: 0 583 1512
Size: 583 929
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 400 432 489 521 745 777
Size: 222 32 146 32 57 32 224 32
Cuts at: 777 1512
Size: 735
Fragments arranged by size:
735 224 222 146 57 32 32 32
32
HincII GTy'rAC
Cuts at: 0 414 1512
Size: 414 1098
HindIII A'AGCT_T
Cuts at: 0 575 902 1512
Size: 575 327 610
Fragments arranged by size:
610 575 327
KpnI G_GTAC'C
Cuts at: 0 783 1110 1337 1512
Size: 783 327 227 175
Fragments arranged by size:
783 327 227 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 758 776 1512
Size: 384 374 18 736
Fragments arranged by size:
736 384 374 18
MslI CAynn'nnrTG
Cuts at: 0 709 790 1280 1512
Size: 709 81 490 232
Fragments arranged by size:
709 490 232 81
MspA1I CmG'CkG
Cuts at: 0 297 617 746 1298 1512
Size: 297 320 129 552 214
Fragments arranged by size:
552 320 297 214 129
NdeI CA'TA_TG
Cuts at: 0 1193 1512
Size: 1193 319
NspI r_CATG'y
Cuts at: 0 1185 1512
Size: 1185 327
PasI CC'CwG_GG
Cuts at: 0 765 1512
Size: 765 747
PfoI T'CCnGG_A
Cuts at: 0 182 1474 1512
Size: 182 1292 38
Fragments arranged by size:
1292 182 38
PstI C_TGCA'G
Cuts at: 0 95 327 1016 1512
Size: 95 232 689 496
Fragments arranged by size:
689 496 232 95
PvuII CAG'CTG
Cuts at: 0 297 746 1512
Size: 297 449 766
Fragments arranged by size:
766 449 297
SacII CC_GC'GG
Cuts at: 0 618 1512
Size: 618 894
SbfI CC_TGCA'GG
Cuts at: 0 95 1016 1512
Size: 95 921 496
Fragments arranged by size:
921 496 95
SfcI C'TryA_G
Cuts at: 0 91 323 1012 1512
Size: 91 232 689 500
Fragments arranged by size:
689 500 232 91
SmaI CCC'GGG
Cuts at: 0 1062 1081 1512
Size: 1062 19 431
Fragments arranged by size:
1062 431 19
StyI C'CwwG_G
Cuts at: 0 1329 1512
Size: 1329 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 757 1379 1512
Size: 757 622 133
Fragments arranged by size:
757 622 133
TatI w'GTAC_w
Cuts at: 0 50 117 1235 1512
Size: 50 67 1118 277
Fragments arranged by size:
1118 277 67 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 844 876 1512
Size: 90 32 722 32 636
Fragments arranged by size:
722 636 90 32 32
XmaI C'CCGG_G
Cuts at: 0 1060 1079 1512
Size: 1060 19 433
Fragments arranged by size:
1060 433 19
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI ApaLI ApoI
AvaI BaeI BanI BanII BglII BlpI Bme1580I BmrI
BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtsI CspCI DraIII
EarI EciI Eco57MI EcoNI EcoO109I FalI FspI Hin4I
HincII HindIII KpnI MmeI MslI MspA1I NdeI NspI
PasI PfoI PstI PvuII SacII SbfI SfcI SmaI
StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI
BmtI Bpu10I BpuEI BsaBI BsaHI BsiWI BsmI BspHI
BsrBI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DrdI EaeI EagI EcoICRI EcoRI
EcoRV FseI FspAI HaeII HpaI KasI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmnI ZraI