Restriction map for
pF1KA1995
(Linear) (Six-base) MAPSORT of: KIAA1995.seq Check: 1789 from: 1 to: 2937
KIAA1995.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2676 2805 2937
Size: 2676 129 132
Fragments arranged by size:
2676 132 129
AccI GT'mk_AC
Cuts at: 0 2925 2937
Size: 2925 12
Acc65I G'GTAC_C
Cuts at: 0 464 2937
Size: 464 2473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1418 2585 2937
Size: 1025 393 1167 352
Fragments arranged by size:
1167 1025 393 352
AflIII A'CryG_T
Cuts at: 0 650 2937
Size: 650 2287
AleI CACnn'nnGTG
Cuts at: 0 1177 2937
Size: 1177 1760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 510 729 1139 2698 2937
Size: 510 219 410 1559 239
Fragments arranged by size:
1559 510 410 239 219
AlwNI CAG_nnn'CTG
Cuts at: 0 1083 2420 2512 2712 2937
Size: 1083 1337 92 200 225
Fragments arranged by size:
1337 1083 225 200 92
ApoI r'AATT_y
Cuts at: 0 797 1678 2184 2458 2937
Size: 797 881 506 274 479
Fragments arranged by size:
881 797 506 479 274
AvaI C'yCGr_G
Cuts at: 0 81 2250 2937
Size: 81 2169 687
Fragments arranged by size:
2169 687 81
BamHI G'GATC_C
Cuts at: 0 873 2868 2937
Size: 873 1995 69
Fragments arranged by size:
1995 873 69
BanI G'GyrC_C
Cuts at: 0 181 464 2900 2937
Size: 181 283 2436 37
Fragments arranged by size:
2436 283 181 37
BanII G_rGCy'C
Cuts at: 0 665 2245 2937
Size: 665 1580 692
Fragments arranged by size:
1580 692 665
BbeI G_GCGC'C
Cuts at: 0 185 2937
Size: 185 2752
BbvCI CC'TCA_GC
Cuts at: 0 1432 2937
Size: 1432 1505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 781 815 2937
Size: 781 34 2122
Fragments arranged by size:
2122 781 34
BclI T'GATC_A
Cuts at: 0 1955 2937
Size: 1955 982
BfrBI ATG'CAT
Cuts at: 0 2826 2937
Size: 2826 111
Bme1580I G_kGCm'C
Cuts at: 0 1113 2937
Size: 1113 1824
BmrI ACTGGGnnnn_n'
Cuts at: 0 1543 1638 2937
Size: 1543 95 1299
Fragments arranged by size:
1543 1299 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 240 1470 2937
Size: 240 1230 1467
Fragments arranged by size:
1467 1230 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 2746 2937
Size: 641 2105 191
Fragments arranged by size:
2105 641 191
Bpu10I CC'TnA_GC
Cuts at: 0 878 1432 2937
Size: 878 554 1505
Fragments arranged by size:
1505 878 554
BsaI GGTCTCn'nnnn_
Cuts at: 0 879 975 1458 1968 2058 2937
Size: 879 96 483 510 90 879
Fragments arranged by size:
879 879 510 483 96 90
BsaBI GATnn'nnATC
Cuts at: 0 2003 2937
Size: 2003 934
BsaHI Gr'CG_yC
Cuts at: 0 182 2937
Size: 182 2755
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1219 1249 2937
Size: 1219 30 1688
Fragments arranged by size:
1688 1219 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2270 2477 2648 2937
Size: 2270 207 171 289
Fragments arranged by size:
2270 289 207 171
BseYI C'CCAG_C
Cuts at: 0 9 1215 1689 1884 2635 2929 2937
Size: 9 1206 474 195 751 294 8
Fragments arranged by size:
1206 751 474 294 195 9 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1145 2516 2694 2937
Size: 133 1012 1371 178 243
Fragments arranged by size:
1371 1012 243 178 133
BsiHKAI G_wGCw'C
Cuts at: 0 665 2245 2937
Size: 665 1580 692
Fragments arranged by size:
1580 692 665
BsmI GAATG_Cn'
Cuts at: 0 2826 2937
Size: 2826 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 619 2937
Size: 619 2318
Bsp1286I G_dGCh'C
Cuts at: 0 665 1113 2245 2937
Size: 665 448 1132 692
Fragments arranged by size:
1132 692 665 448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1107 1440 2676 2805 2937
Size: 1107 333 1236 129 132
Fragments arranged by size:
1236 1107 333 132 129
BsrDI GCAATG_nn'
Cuts at: 0 1013 1802 1985 2031 2054 2937
Size: 1013 789 183 46 23 883
Fragments arranged by size:
1013 883 789 183 46 23
BsrFI r'CCGG_y
Cuts at: 0 118 1847 2937
Size: 118 1729 1090
Fragments arranged by size:
1729 1090 118
BsrGI T'GTAC_A
Cuts at: 0 1169 2937
Size: 1169 1768
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1015 2937
Size: 1015 1922
BstBI TT'CG_AA
Cuts at: 0 2436 2937
Size: 2436 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 508 2937
Size: 508 2429
BstYI r'GATC_y
Cuts at: 0 873 1793 2113 2868 2937
Size: 873 920 320 755 69
Fragments arranged by size:
920 873 755 320 69
BtgI C'CryG_G
Cuts at: 0 177 617 806 1206 1221 2145 2390 2937
Size: 177 440 189 400 15 924 245 547
Fragments arranged by size:
924 547 440 400 245 189 177 15
BtsI GCAGTG_nn'
Cuts at: 0 27 310 494 2217 2937
Size: 27 283 184 1723 720
Fragments arranged by size:
1723 720 283 184 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1238 1273 1857 1892 2193 2228 2937
Size: 1238 35 584 35 301 35 709
Fragments arranged by size:
1238 709 584 301 35 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 261 2937
Size: 261 2676
EaeI y'GGCC_r
Cuts at: 0 1769 2037 2937
Size: 1769 268 900
Fragments arranged by size:
1769 900 268
EarI CTCTTCn'nnn_
Cuts at: 0 443 923 1544 2279 2531 2849 2937
Size: 443 480 621 735 252 318 88
Fragments arranged by size:
735 621 480 443 318 252 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 148 2937
Size: 148 2789
EcoICRI GAG'CTC
Cuts at: 0 663 2243 2937
Size: 663 1580 694
Fragments arranged by size:
1580 694 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1025 1418 2585 2746 2937
Size: 641 384 393 1167 161 191
Fragments arranged by size:
1167 641 393 384 191 161
EcoNI CCTnn'n_nnAGG
Cuts at: 0 741 868 2937
Size: 741 127 2069
Fragments arranged by size:
2069 741 127
EcoO109I rG'GnC_Cy
Cuts at: 0 91 1933 2937
Size: 91 1842 1004
Fragments arranged by size:
1842 1004 91
EcoRI G'AATT_C
Cuts at: 0 797 1678 2184 2937
Size: 797 881 506 753
Fragments arranged by size:
881 797 753 506
EcoRV GAT'ATC
Cuts at: 0 699 2082 2937
Size: 699 1383 855
Fragments arranged by size:
1383 855 699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2898 2937
Size: 2898 39
HaeII r_GCGC'y
Cuts at: 0 101 185 2937
Size: 101 84 2752
Fragments arranged by size:
2752 101 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 2467 2499 2918 2937
Size: 1219 32 1216 32 419 19
Fragments arranged by size:
1219 1216 419 32 32 19
HincII GTy'rAC
Cuts at: 0 816 1640 2937
Size: 816 824 1297
Fragments arranged by size:
1297 824 816
KasI G'GCGC_C
Cuts at: 0 181 2937
Size: 181 2756
KpnI G_GTAC'C
Cuts at: 0 468 2937
Size: 468 2469
MscI TGG'CCA
Cuts at: 0 1771 2937
Size: 1771 1166
MslI CAynn'nnrTG
Cuts at: 0 1177 1375 2937
Size: 1177 198 1562
Fragments arranged by size:
1562 1177 198
MspA1I CmG'CkG
Cuts at: 0 179 1215 1419 1828 1884 2937
Size: 179 1036 204 409 56 1053
Fragments arranged by size:
1053 1036 409 204 179 56
NaeI GCC'GGC
Cuts at: 0 120 1849 2937
Size: 120 1729 1088
Fragments arranged by size:
1729 1088 120
NarI GG'CG_CC
Cuts at: 0 182 2937
Size: 182 2755
NcoI C'CATG_G
Cuts at: 0 617 806 1206 1221 2390 2937
Size: 617 189 400 15 1169 547
Fragments arranged by size:
1169 617 547 400 189 15
NgoMIV G'CCGG_C
Cuts at: 0 118 1847 2937
Size: 118 1729 1090
Fragments arranged by size:
1729 1090 118
NsiI A_TGCA'T
Cuts at: 0 2828 2937
Size: 2828 109
NspI r_CATG'y
Cuts at: 0 654 1188 1258 1710 2937
Size: 654 534 70 452 1227
Fragments arranged by size:
1227 654 534 452 70
PciI A'CATG_T
Cuts at: 0 650 2937
Size: 650 2287
PflMI CCAn_nnn'nTGG
Cuts at: 0 1083 2937
Size: 1083 1854
PfoI T'CCnGG_A
Cuts at: 0 2129 2937
Size: 2129 808
PpuMI rG'GwC_Cy
Cuts at: 0 1933 2937
Size: 1933 1004
PshAI GACnn'nnGTC
Cuts at: 0 83 987 2937
Size: 83 904 1950
Fragments arranged by size:
1950 904 83
PsiI TTA'TAA
Cuts at: 0 1790 2937
Size: 1790 1147
PspXI vC'TCGA_Gb
Cuts at: 0 81 2937
Size: 81 2856
PstI C_TGCA'G
Cuts at: 0 320 897 2625 2687 2783 2937
Size: 320 577 1728 62 96 154
Fragments arranged by size:
1728 577 320 154 96 62
PvuII CAG'CTG
Cuts at: 0 1215 1419 1828 1884 2937
Size: 1215 204 409 56 1053
Fragments arranged by size:
1215 1053 409 204 56
SacI G_AGCT'C
Cuts at: 0 665 2245 2937
Size: 665 1580 692
Fragments arranged by size:
1580 692 665
SacII CC_GC'GG
Cuts at: 0 180 2937
Size: 180 2757
SanDI GG'GwC_CC
Cuts at: 0 1933 2937
Size: 1933 1004
SapI GCTCTTCn'nnn_
Cuts at: 0 2531 2937
Size: 2531 406
ScaI AGT'ACT
Cuts at: 0 829 2937
Size: 829 2108
SfcI C'TryA_G
Cuts at: 0 316 893 1019 1104 2621 2683 2746 2779
Size: 316 577 126 85 1517 62 63 33
Cuts at: 2779 2922 2937
Size: 143 15
Fragments arranged by size:
1517 577 316 143 126 85 63 62
33 15
SfoI GGC'GCC
Cuts at: 0 183 2937
Size: 183 2754
SmaI CCC'GGG
Cuts at: 0 2252 2937
Size: 2252 685
SmlI C'TyrA_G
Cuts at: 0 81 2937
Size: 81 2856
SspI AAT'ATT
Cuts at: 0 388 543 2937
Size: 388 155 2394
Fragments arranged by size:
2394 388 155
StuI AGG'CCT
Cuts at: 0 1591 2937
Size: 1591 1346
StyI C'CwwG_G
Cuts at: 0 554 617 806 985 1206 1221 1586 1766
Size: 554 63 189 179 221 15 365 180
Cuts at: 1766 1938 2390 2937
Size: 172 452 547
Fragments arranged by size:
554 547 452 365 221 189 180 179
172 63 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1026 1167 2369 2937
Size: 1026 141 1202 568
Fragments arranged by size:
1202 1026 568 141
TatI w'GTAC_w
Cuts at: 0 680 827 1169 2937
Size: 680 147 342 1768
Fragments arranged by size:
1768 680 342 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 2540 2572 2937
Size: 19 32 2489 32 365
Fragments arranged by size:
2489 365 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 0 1644 2937
Size: 1644 1293
XbaI T'CTAG_A
Cuts at: 0 906 2937
Size: 906 2031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 2388 2937
Size: 168 2220 549
Fragments arranged by size:
2220 549 168
XhoI C'TCGA_G
Cuts at: 0 81 2937
Size: 81 2856
XmaI C'CCGG_G
Cuts at: 0 2250 2937
Size: 2250 687
XmnI GAAnn'nnTTC
Cuts at: 0 1426 2937
Size: 1426 1511
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BclI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstBI
BstXI BstYI BtgI BtsI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PpuMI PshAI PsiI PspXI PstI PvuII SacI
SacII SanDI SapI ScaI SfcI SfoI SmaI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbsI BciVI
BglI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaWI
BsiEI BsiWI BspEI BspHI BsrBI BssHII BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI FseI
FspI FspAI HindIII HpaI MfeI MluI MmeI NdeI
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PspOMI PsrI PvuI RsrII SalI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI ZraI