Restriction map for
pF1KA1997
(Linear) (Six-base) MAPSORT of: KIAA1997.seq Check: 7919 from: 1 to: 12921
KIAA1997.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1188 12921
Size: 1188 11733
AccI GT'mk_AC
Cuts at: 0 4330 4951 7034 12618 12921
Size: 4330 621 2083 5584 303
Fragments arranged by size:
5584 4330 2083 621 303
AclI AA'CG_TT
Cuts at: 0 11133 12921
Size: 11133 1788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 385 2990 3115 4381 4664 4883 7381
Size: 269 116 2605 125 1266 283 219 2498
Cuts at: 7381 8080 8177 8887 9379 9389 9440 9500 11497
Size: 699 97 710 492 10 51 60 1997
Cuts at: 11497 11577 11803 11834 11851 12921
Size: 80 226 31 17 1070
Fragments arranged by size:
2605 2498 1997 1266 1070 710 699 492
283 269 226 219 125 116 97 80
60 51 31 17 10
AfeI AGC'GCT
Cuts at: 0 922 2149 12921
Size: 922 1227 10772
Fragments arranged by size:
10772 1227 922
AflII C'TTAA_G
Cuts at: 0 6727 8251 12921
Size: 6727 1524 4670
Fragments arranged by size:
6727 4670 1524
AflIII A'CryG_T
Cuts at: 0 4915 8085 8628 10541 10796 12921
Size: 4915 3170 543 1913 255 2125
Fragments arranged by size:
4915 3170 2125 1913 543 255
AhdI GACnn_n'nnGTC
Cuts at: 0 7701 12921
Size: 7701 5220
AleI CACnn'nnGTG
Cuts at: 0 96 12921
Size: 96 12825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 6910 12820 12921
Size: 160 6750 5910 101
Fragments arranged by size:
6750 5910 160 101
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 10613 10645 12921
Size: 770 32 9811 32 2276
Fragments arranged by size:
9811 2276 770 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 93 6990 8800 9463 12921
Size: 93 6897 1810 663 3458
Fragments arranged by size:
6897 3458 1810 663 93
ApaLI G'TGCA_C
Cuts at: 0 6956 10403 12921
Size: 6956 3447 2518
Fragments arranged by size:
6956 3447 2518
ApoI r'AATT_y
Cuts at: 0 478 1226 1394 1825 1953 2310 2652 3049
Size: 478 748 168 431 128 357 342 397
Cuts at: 3049 3157 3386 3481 4020 4194 4249 4636 4844
Size: 108 229 95 539 174 55 387 208
Cuts at: 4844 4942 5224 5343 5357 6198 6559 6619 7170
Size: 98 282 119 14 841 361 60 551
Cuts at: 7170 7394 7447 7869 8312 8355 8532 9005 9314
Size: 224 53 422 443 43 177 473 309
Cuts at: 9314 9439 9709 10093 10445 11514 11668 11746 11957
Size: 125 270 384 352 1069 154 78 211
Cuts at: 11957 12088 12156 12595 12921
Size: 131 68 439 326
Fragments arranged by size:
1069 841 748 551 539 478 473 443
439 431 422 397 387 384 361 357
352 342 326 309 282 270 229 224
211 208 177 174 168 154 131 128
125 119 108 98 95 78 68 60
55 53 43 14
AseI AT'TA_AT
Cuts at: 0 2729 3832 6569 8569 9527 10535 12921
Size: 2729 1103 2737 2000 958 1008 2386
Fragments arranged by size:
2737 2729 2386 2000 1103 1008 958
AvaI C'yCGr_G
Cuts at: 0 1076 12921
Size: 1076 11845
AvrII C'CTAG_G
Cuts at: 0 3658 12921
Size: 3658 9263
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2828 2861 3704 3737 7411 7444 12921
Size: 2828 33 843 33 3674 33 5477
Fragments arranged by size:
5477 3674 2828 843 33 33 33
BamHI G'GATC_C
Cuts at: 0 4746 7540 12921
Size: 4746 2794 5381
Fragments arranged by size:
5381 4746 2794
BanI G'GyrC_C
Cuts at: 0 1385 11725 12921
Size: 1385 10340 1196
Fragments arranged by size:
10340 1385 1196
BanII G_rGCy'C
Cuts at: 0 1136 7684 7734 8237 9612 10590 11583 12921
Size: 1136 6548 50 503 1375 978 993 1338
Fragments arranged by size:
6548 1375 1338 1136 993 978 503 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 2752 2978 3121 3446 3851 4669 4871 5127
Size: 2752 226 143 325 405 818 202 256
Cuts at: 5127 5406 5614 6746 7236 7245 8183 8283 9140
Size: 279 208 1132 490 9 938 100 857
Cuts at: 9140 9377 12921
Size: 237 3544
Fragments arranged by size:
3544 2752 1132 938 857 818 490 405
325 279 256 237 226 208 202 143
100 9
BbvCI CC'TCA_GC
Cuts at: 0 5573 12921
Size: 5573 7348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3361 3395 10708 10742 12921
Size: 3361 34 7313 34 2179
Fragments arranged by size:
7313 3361 2179 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 514 6675 10761 12921
Size: 514 6161 4086 2160
Fragments arranged by size:
6161 4086 2160 514
BclI T'GATC_A
Cuts at: 0 720 1881 4215 4908 8174 8813 10455 10698
Size: 720 1161 2334 693 3266 639 1642 243
Cuts at: 10698 10833 12026 12921
Size: 135 1193 895
Fragments arranged by size:
3266 2334 1642 1193 1161 895 720 693
639 243 135
BfrBI ATG'CAT
Cuts at: 0 1408 2226 2230 8013 10737 12769 12921
Size: 1408 818 4 5783 2724 2032 152
Fragments arranged by size:
5783 2724 2032 1408 818 152 4
BglII A'GATC_T
Cuts at: 0 1563 1648 2532 3134 6360 10026 11682 12921
Size: 1563 85 884 602 3226 3666 1656 1239
Fragments arranged by size:
3666 3226 1656 1563 1239 884 602 85
BlpI GC'TnA_GC
Cuts at: 0 2184 4269 9236 12921
Size: 2184 2085 4967 3685
Fragments arranged by size:
4967 3685 2184 2085
Bme1580I G_kGCm'C
Cuts at: 0 3384 3762 6648 6960 9832 10407 11313 12444
Size: 3384 378 2886 312 2872 575 906 1131
Cuts at: 12444 12921
Size: 477
Fragments arranged by size:
3384 2886 2872 1131 906 575 477 378
312
BmgBI CAC'GTC
Cuts at: 0 3127 12921
Size: 3127 9794
BmrI ACTGGGnnnn_n'
Cuts at: 0 2874 4571 4878 5087 6230 7243 10895 12468
Size: 2874 1697 307 209 1143 1013 3652 1573
Cuts at: 12468 12921
Size: 453
Fragments arranged by size:
3652 2874 1697 1573 1143 1013 453 307
209
BmtI G_CTAG'C
Cuts at: 0 10300 12921
Size: 10300 2621
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 11344 12816 12921
Size: 11344 1472 105
Fragments arranged by size:
11344 1472 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2290 3602 4795 6644 7244 7961 8774 11483
Size: 2290 1312 1193 1849 600 717 813 2709
Cuts at: 11483 12921
Size: 1438
Fragments arranged by size:
2709 2290 1849 1438 1312 1193 813 717
600
Bpu10I CC'TnA_GC
Cuts at: 0 1929 5573 6901 11546 12921
Size: 1929 3644 1328 4645 1375
Fragments arranged by size:
4645 3644 1929 1375 1328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4559 5012 6119 7804 8155 9493 9724 10022
Size: 4559 453 1107 1685 351 1338 231 298
Cuts at: 10022 10325 12921
Size: 303 2596
Fragments arranged by size:
4559 2596 1685 1338 1107 453 351 303
298 231
BsaAI yAC'GTr
Cuts at: 0 3793 10831 12921
Size: 3793 7038 2090
Fragments arranged by size:
7038 3793 2090
BsaBI GATnn'nnATC
Cuts at: 0 266 725 2906 3311 6215 9073 9348 10472
Size: 266 459 2181 405 2904 2858 275 1124
Cuts at: 10472 12686 12921
Size: 2214 235
Fragments arranged by size:
2904 2858 2214 2181 1124 459 405 275
266 235
BsaWI w'CCGG_w
Cuts at: 0 6923 10140 11046 12786 12921
Size: 6923 3217 906 1740 135
Fragments arranged by size:
6923 3217 1740 906 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2263 2293 4433 4463 7894 7924 8416 8446
Size: 2263 30 2140 30 3431 30 492 30
Cuts at: 8446 12921
Size: 4475
Fragments arranged by size:
4475 3431 2263 2140 492 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 6647 9406 9735 12176 12921
Size: 139 6508 2759 329 2441 745
Fragments arranged by size:
6508 2759 2441 745 329 139
BseYI C'CCAG_C
Cuts at: 0 427 10868 11692 12718 12921
Size: 427 10441 824 1026 203
Fragments arranged by size:
10441 1026 824 427 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1754 4601 9242 12921
Size: 178 1576 2847 4641 3679
Fragments arranged by size:
4641 3679 2847 1576 178
BsiEI CG_ry'CG
Cuts at: 0 4071 12198 12921
Size: 4071 8127 723
Fragments arranged by size:
8127 4071 723
BsiHKAI G_wGCw'C
Cuts at: 0 6114 6960 7734 9612 10407 10590 11583 12737
Size: 6114 846 774 1878 795 183 993 1154
Cuts at: 12737 12921
Size: 184
Fragments arranged by size:
6114 1878 1154 993 846 795 774 184
183
BsmI GAATG_Cn'
Cuts at: 0 1258 2423 4273 5676 7126 7490 9237 9564
Size: 1258 1165 1850 1403 1450 364 1747 327
Cuts at: 9564 12921
Size: 3357
Fragments arranged by size:
3357 1850 1747 1450 1403 1258 1165 364
327
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3649 10014 12921
Size: 3649 6365 2907
Fragments arranged by size:
6365 3649 2907
Bsp1286I G_dGCh'C
Cuts at: 0 1136 3384 3762 6114 6648 6960 7684 7734
Size: 1136 2248 378 2352 534 312 724 50
Cuts at: 7734 8237 9612 9832 10407 10590 11313 11583 12444
Size: 503 1375 220 575 183 723 270 861
Cuts at: 12444 12737 12921
Size: 293 184
Fragments arranged by size:
2352 2248 1375 1136 861 724 723 575
534 503 378 312 293 270 220 184
183 50
BspEI T'CCGG_A
Cuts at: 0 12786 12921
Size: 12786 135
BspHI T'CATG_A
Cuts at: 0 1020 6288 6552 8366 12921
Size: 1020 5268 264 1814 4555
Fragments arranged by size:
5268 4555 1814 1020 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1053 1188 4022 5823 12921
Size: 1053 135 2834 1801 7098
Fragments arranged by size:
7098 2834 1801 1053 135
BsrBI CCG'CTC
Cuts at: 0 8020 12921
Size: 8020 4901
BsrDI GCAATG_nn'
Cuts at: 0 1814 4078 4430 11322 12921
Size: 1814 2264 352 6892 1599
Fragments arranged by size:
6892 2264 1814 1599 352
BsrGI T'GTAC_A
Cuts at: 0 2258 2634 8151 11736 11751 12921
Size: 2258 376 5517 3585 15 1170
Fragments arranged by size:
5517 3585 2258 1170 376 15
BssSI C'ACGA_G
Cuts at: 0 497 6647 12921
Size: 497 6150 6274
Fragments arranged by size:
6274 6150 497
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1816 8325 8596 8800 10402 12921
Size: 1816 6509 271 204 1602 2519
Fragments arranged by size:
6509 2519 1816 1602 271 204
BstEII G'GTnAC_C
Cuts at: 0 12840 12921
Size: 12840 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3626 5040 7119 12921
Size: 3626 1414 2079 5802
Fragments arranged by size:
5802 3626 2079 1414
BstYI r'GATC_y
Cuts at: 0 822 1563 1648 2532 3134 4746 6360 7411
Size: 822 741 85 884 602 1612 1614 1051
Cuts at: 7411 7540 9843 10026 11682 12067 12205 12405 12921
Size: 129 2303 183 1656 385 138 200 516
Fragments arranged by size:
2303 1656 1614 1612 1051 884 822 741
602 516 385 200 183 138 129 85
BstZ17I GTA'TAC
Cuts at: 0 4952 7035 12921
Size: 4952 2083 5886
Fragments arranged by size:
5886 4952 2083
Bsu36I CC'TnA_GG
Cuts at: 0 151 11645 12921
Size: 151 11494 1276
Fragments arranged by size:
11494 1276 151
BtgI C'CryG_G
Cuts at: 0 552 937 4071 6829 10874 11066 11261 12921
Size: 552 385 3134 2758 4045 192 195 1660
Fragments arranged by size:
4045 3134 2758 1660 552 385 195 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 533 12921
Size: 533 12388
BtsI GCAGTG_nn'
Cuts at: 0 3827 7974 10407 11113 11426 12575 12921
Size: 3827 4147 2433 706 313 1149 346
Fragments arranged by size:
4147 3827 2433 1149 706 346 313
ClaI AT'CG_AT
Cuts at: 0 5156 12921
Size: 5156 7765
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1325 1360 6918 6953 12551 12586 12862 12897
Size: 1325 35 5558 35 5598 35 276 35
Cuts at: 12897 12921
Size: 24
Fragments arranged by size:
5598 5558 1325 276 35 35 35 35
24
DraI TTT'AAA
Cuts at: 0 594 2658 2723 3162 3749 4025 4458 5505
Size: 594 2064 65 439 587 276 433 1047
Cuts at: 5505 9204 11507 12921
Size: 3699 2303 1414
Fragments arranged by size:
3699 2303 2064 1414 1047 594 587 439
433 276 65
DraIII CAC_nnn'GTG
Cuts at: 0 3766 12921
Size: 3766 9155
DrdI GACnn_nn'nnGTC
Cuts at: 0 4159 12921
Size: 4159 8762
EaeI y'GGCC_r
Cuts at: 0 3210 3431 4068 12921
Size: 3210 221 637 8853
Fragments arranged by size:
8853 3210 637 221
EagI C'GGCC_G
Cuts at: 0 4068 12921
Size: 4068 8853
EarI CTCTTCn'nnn_
Cuts at: 0 37 989 1049 2404 3323 3398 5837 6347
Size: 37 952 60 1355 919 75 2439 510
Cuts at: 6347 6782 9602 10322 11399 11596 12491 12551 12921
Size: 435 2820 720 1077 197 895 60 370
Fragments arranged by size:
2820 2439 1355 1077 952 919 895 720
510 435 370 197 75 60 60 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4639 10087 12921
Size: 4639 5448 2834
Fragments arranged by size:
5448 4639 2834
EcoICRI GAG'CTC
Cuts at: 0 7732 9610 10588 11581 12921
Size: 7732 1878 978 993 1340
Fragments arranged by size:
7732 1878 1340 993 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 385 2290 2990 3115 3602 4381 4664
Size: 269 116 1905 700 125 487 779 283
Cuts at: 4664 4795 4883 6644 7244 7381 7961 8080 8177
Size: 131 88 1761 600 137 580 119 97
Cuts at: 8177 8774 8887 9379 9389 9440 9500 11483 11497
Size: 597 113 492 10 51 60 1983 14
Cuts at: 11497 11577 11803 11834 11851 12921
Size: 80 226 31 17 1070
Fragments arranged by size:
1983 1905 1761 1070 779 700 600 597
580 492 487 283 269 226 137 131
125 119 116 113 97 88 80 60
51 31 17 14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 994 3475 7135 12921
Size: 994 2481 3660 5786
Fragments arranged by size:
5786 3660 2481 994
EcoO109I rG'GnC_Cy
Cuts at: 0 2366 5204 12921
Size: 2366 2838 7717
Fragments arranged by size:
7717 2838 2366
EcoRI G'AATT_C
Cuts at: 0 1394 1953 6198 6559 8312 9314 10093 12156
Size: 1394 559 4245 361 1753 1002 779 2063
Cuts at: 12156 12921
Size: 765
Fragments arranged by size:
4245 2063 1753 1394 1002 779 765 559
361
EcoRV GAT'ATC
Cuts at: 0 3369 4143 6046 7080 12921
Size: 3369 774 1903 1034 5841
Fragments arranged by size:
5841 3369 1903 1034 774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1343 5031 5853 9214 10333 10438 10524 12921
Size: 1343 3688 822 3361 1119 105 86 2397
Fragments arranged by size:
3688 3361 2397 1343 1119 822 105 86
HaeII r_GCGC'y
Cuts at: 0 924 2151 7599 12921
Size: 924 1227 5448 5322
Fragments arranged by size:
5448 5322 1227 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 1597 1612 1629 1633 1644 1665
Size: 628 32 937 15 17 4 11 21
Cuts at: 1665 1884 1916 2745 2777 3054 3086 3126 3158
Size: 219 32 829 32 277 32 40 32
Cuts at: 3158 3908 3940 3958 3990 4111 4143 4338 4370
Size: 750 32 18 32 121 32 195 32
Cuts at: 4370 4390 4422 5149 5181 6130 6162 6177 6209
Size: 20 32 727 32 949 32 15 32
Cuts at: 6209 7666 7687 7698 7719 7894 7926 8416 8448
Size: 1457 21 11 21 175 32 490 32
Cuts at: 8448 10011 10043 10969 11001 12018 12050 12921
Size: 1563 32 926 32 1017 32 871
Fragments arranged by size:
1563 1457 1017 949 937 926 871 829
750 727 628 490 277 219 195 175
121 40 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 21 21 21 20
18 17 15 15 11 11 4
HincII GTy'rAC
Cuts at: 0 663 1449 3561 4587 6270 10112 10170 12619
Size: 663 786 2112 1026 1683 3842 58 2449
Cuts at: 12619 12921
Size: 302
Fragments arranged by size:
3842 2449 2112 1683 1026 786 663 302
58
HindIII A'AGCT_T
Cuts at: 0 3785 4366 8261 12921
Size: 3785 581 3895 4660
Fragments arranged by size:
4660 3895 3785 581
HpaI GTT'AAC
Cuts at: 0 6270 10170 12921
Size: 6270 3900 2751
Fragments arranged by size:
6270 3900 2751
MfeI C'AATT_G
Cuts at: 0 611 3716 12921
Size: 611 3105 9205
Fragments arranged by size:
9205 3105 611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 212 1315 1454 3795 3885 7963 8955
Size: 191 21 1103 139 2341 90 4078 992
Cuts at: 8955 10596 10605 10770 11632 12921
Size: 1641 9 165 862 1289
Fragments arranged by size:
4078 2341 1641 1289 1103 992 862 191
165 139 90 21 9
MscI TGG'CCA
Cuts at: 0 3212 3433 12921
Size: 3212 221 9488
Fragments arranged by size:
9488 3212 221
MslI CAynn'nnrTG
Cuts at: 0 96 1553 2825 5038 5158 6536 6953 8012
Size: 96 1457 1272 2213 120 1378 417 1059
Cuts at: 8012 12921
Size: 4909
Fragments arranged by size:
4909 2213 1457 1378 1272 1059 417 120
96
MspA1I CmG'CkG
Cuts at: 0 2554 5071 7222 9256 9697 12079 12921
Size: 2554 2517 2151 2034 441 2382 842
Fragments arranged by size:
2554 2517 2382 2151 2034 842 441
NcoI C'CATG_G
Cuts at: 0 937 6829 11066 11261 12921
Size: 937 5892 4237 195 1660
Fragments arranged by size:
5892 4237 1660 937 195
NdeI CA'TA_TG
Cuts at: 0 1066 6720 7584 8592 10658 11487 12921
Size: 1066 5654 864 1008 2066 829 1434
Fragments arranged by size:
5654 2066 1434 1066 1008 864 829
NheI G'CTAG_C
Cuts at: 0 10296 12921
Size: 10296 2625
NsiI A_TGCA'T
Cuts at: 0 1410 2228 2232 8015 10739 12771 12921
Size: 1410 818 4 5783 2724 2032 150
Fragments arranged by size:
5783 2724 2032 1410 818 150 4
NspI r_CATG'y
Cuts at: 0 2226 2230 4919 6804 8013 8089 8632 9024
Size: 2226 4 2689 1885 1209 76 543 392
Cuts at: 9024 10545 10737 10800 11084 11621 12773 12921
Size: 1521 192 63 284 537 1152 148
Fragments arranged by size:
2689 2226 1885 1521 1209 1152 543 537
392 284 192 148 76 63 4
PacI TTA_AT'TAA
Cuts at: 0 9527 12921
Size: 9527 3394
PciI A'CATG_T
Cuts at: 0 4915 8085 8628 10541 10796 12921
Size: 4915 3170 543 1913 255 2125
Fragments arranged by size:
4915 3170 2125 1913 543 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 1423 2886 5916 9306 12921
Size: 1423 1463 3030 3390 3615
Fragments arranged by size:
3615 3390 3030 1463 1423
PfoI T'CCnGG_A
Cuts at: 0 4772 5778 8205 12921
Size: 4772 1006 2427 4716
Fragments arranged by size:
4772 4716 2427 1006
PsiI TTA'TAA
Cuts at: 0 629 1871 3953 10955 12921
Size: 629 1242 2082 7002 1966
Fragments arranged by size:
7002 2082 1966 1242 629
PspXI vC'TCGA_Gb
Cuts at: 0 1076 12921
Size: 1076 11845
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 7331 7363 9986 10018 12921
Size: 7331 32 2623 32 2903
Fragments arranged by size:
7331 2903 2623 32 32
PstI C_TGCA'G
Cuts at: 0 4019 6986 9255 9516 11433 11926 12921
Size: 4019 2967 2269 261 1917 493 995
Fragments arranged by size:
4019 2967 2269 1917 995 493 261
PvuI CG_AT'CG
Cuts at: 0 12198 12921
Size: 12198 723
PvuII CAG'CTG
Cuts at: 0 2554 5071 7222 9256 9697 12079 12921
Size: 2554 2517 2151 2034 441 2382 842
Fragments arranged by size:
2554 2517 2382 2151 2034 842 441
SacI G_AGCT'C
Cuts at: 0 7734 9612 10590 11583 12921
Size: 7734 1878 978 993 1338
Fragments arranged by size:
7734 1878 1338 993 978
SalI G'TCGA_C
Cuts at: 0 12617 12921
Size: 12617 304
SapI GCTCTTCn'nnn_
Cuts at: 0 37 3323 12491 12551 12921
Size: 37 3286 9168 60 370
Fragments arranged by size:
9168 3286 370 60 37
ScaI AGT'ACT
Cuts at: 0 7017 7116 9249 12921
Size: 7017 99 2133 3672
Fragments arranged by size:
7017 3672 2133 99
SexAI A'CCwGG_T
Cuts at: 0 11252 12921
Size: 11252 1669
SfcI C'TryA_G
Cuts at: 0 2192 4015 6941 6982 7286 9251 9512 9860
Size: 2192 1823 2926 41 304 1965 261 348
Cuts at: 9860 11429 11464 11922 12921
Size: 1569 35 458 999
Fragments arranged by size:
2926 2192 1965 1823 1569 999 458 348
304 261 41 35
SmlI C'TyrA_G
Cuts at: 0 1076 4574 5027 6134 6727 7819 8134 8251
Size: 1076 3498 453 1107 593 1092 315 117
Cuts at: 8251 9472 9703 10037 10340 12921
Size: 1221 231 334 303 2581
Fragments arranged by size:
3498 2581 1221 1107 1092 1076 593 453
334 315 303 231 117
SpeI A'CTAG_T
Cuts at: 0 3667 12921
Size: 3667 9254
SphI G_CATG'C
Cuts at: 0 2230 9024 10737 12921
Size: 2230 6794 1713 2184
Fragments arranged by size:
6794 2230 2184 1713
SspI AAT'ATT
Cuts at: 0 955 2124 2395 2727 4319 4818 5180 7197
Size: 955 1169 271 332 1592 499 362 2017
Cuts at: 7197 9162 9288 9812 10350 12849 12921
Size: 1965 126 524 538 2499 72
Fragments arranged by size:
2499 2017 1965 1592 1169 955 538 524
499 362 332 271 126 72
StuI AGG'CCT
Cuts at: 0 9770 12921
Size: 9770 3151
StyI C'CwwG_G
Cuts at: 0 937 3648 3658 6829 11066 11261 12921
Size: 937 2711 10 3171 4237 195 1660
Fragments arranged by size:
4237 3171 2711 1660 937 195 10
SwaI ATTT'AAAT
Cuts at: 0 3749 4025 12921
Size: 3749 276 8896
Fragments arranged by size:
8896 3749 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 870 2437 4035 9081 9264 12921
Size: 870 1567 1598 5046 183 3657
Fragments arranged by size:
5046 3657 1598 1567 870 183
TatI w'GTAC_w
Cuts at: 0 2258 2634 5247 7015 7067 7114 8151 8940
Size: 2258 376 2613 1768 52 47 1037 789
Cuts at: 8940 9247 9733 10505 11031 11736 11751 12258 12330
Size: 307 486 772 526 705 15 507 72
Cuts at: 12330 12921
Size: 591
Fragments arranged by size:
2613 2258 1768 1037 789 772 705 591
526 507 486 376 307 72 52 47
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 488 520 2192 2224 5358 5390 6144 6176
Size: 488 32 1672 32 3134 32 754 32
Cuts at: 6176 12876 12908 12921
Size: 6700 32 13
Fragments arranged by size:
6700 3134 1672 754 488 32 32 32
32 32 13
Tth111I GACn'n_nGTC
Cuts at: 0 8681 9690 12921
Size: 8681 1009 3231
Fragments arranged by size:
8681 3231 1009
XbaI T'CTAG_A
Cuts at: 0 3672 6322 6813 7188 10320 12921
Size: 3672 2650 491 375 3132 2601
Fragments arranged by size:
3672 3132 2650 2601 491 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3184 7507 12921
Size: 3184 4323 5414
Fragments arranged by size:
5414 4323 3184
XhoI C'TCGA_G
Cuts at: 0 1076 12921
Size: 1076 11845
XmnI GAAnn'nnTTC
Cuts at: 0 31 58 2413 2693 3407 5483 6248 7196
Size: 31 27 2355 280 714 2076 765 948
Cuts at: 7196 7343 8065 9106 9162 9194 9314 9440 10001
Size: 147 722 1041 56 32 120 126 561
Cuts at: 10001 10316 10748 12921
Size: 315 432 2173
Fragments arranged by size:
2355 2173 2076 1041 948 765 722 714
561 432 315 280 147 126 120 56
32 31 27
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AvaI
AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI
MscI MslI MspA1I NcoI NdeI NheI NsiI NspI
PacI PciI PflMI PfoI PsiI PspXI PsrI PstI
PvuI PvuII SacI SalI SapI ScaI SexAI SfcI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI ApaI AscI AsiSI BbeI BglI
BsaI BsaHI BsiWI BsrFI BssHII BstBI FseI FspI
FspAI KasI KpnI MluI NaeI NarI NgoMIV NotI
NruI PasI PmeI PmlI PpiI PpuMI PshAI PspOMI
RsrII SacII SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI XmaI ZraI