Restriction map for
pF1KA2009
(Linear) (Six-base) MAPSORT of: KIAA2009.seq Check: 2605 from: 1 to: 1707
KIAA2009.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
Acc65I G'GTAC_C
Cuts at: 0 498 1707
Size: 498 1209
AfeI AGC'GCT
Cuts at: 0 272 1707
Size: 272 1435
AflIII A'CryG_T
Cuts at: 0 572 1466 1707
Size: 572 894 241
Fragments arranged by size:
894 572 241
AleI CACnn'nnGTG
Cuts at: 0 739 1707
Size: 739 968
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 595 627 1707
Size: 595 32 1080
Fragments arranged by size:
1080 595 32
AlwNI CAG_nnn'CTG
Cuts at: 0 791 942 1707
Size: 791 151 765
Fragments arranged by size:
791 765 151
ApaI G_GGCC'C
Cuts at: 0 533 1134 1707
Size: 533 601 573
Fragments arranged by size:
601 573 533
ApaLI G'TGCA_C
Cuts at: 0 1282 1707
Size: 1282 425
AvaI C'yCGr_G
Cuts at: 0 472 650 1069 1149 1234 1344 1389 1654
Size: 472 178 419 80 85 110 45 265
Cuts at: 1654 1707
Size: 53
Fragments arranged by size:
472 419 265 178 110 85 80 53
45
BanI G'GyrC_C
Cuts at: 0 151 450 498 538 733 1116 1243 1303
Size: 151 299 48 40 195 383 127 60
Cuts at: 1303 1590 1707
Size: 287 117
Fragments arranged by size:
383 299 287 195 151 127 117 60
48 40
BanII G_rGCy'C
Cuts at: 0 99 521 533 704 1134 1477 1655 1707
Size: 99 422 12 171 430 343 178 52
Fragments arranged by size:
430 422 343 178 171 99 52 12
BbeI G_GCGC'C
Cuts at: 0 155 542 1247 1707
Size: 155 387 705 460
Fragments arranged by size:
705 460 387 155
BbsI GAAGACnn'nnnn_
Cuts at: 0 289 1221 1707
Size: 289 932 486
Fragments arranged by size:
932 486 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1561 1595 1707
Size: 1561 34 112
Fragments arranged by size:
1561 112 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1348 1707
Size: 1348 359
Bme1580I G_kGCm'C
Cuts at: 0 533 1119 1134 1286 1440 1595 1663 1707
Size: 533 586 15 152 154 155 68 44
Fragments arranged by size:
586 533 155 154 152 68 44 15
BmgBI CAC'GTC
Cuts at: 0 1469 1707
Size: 1469 238
BmrI ACTGGGnnnn_n'
Cuts at: 0 212 301 1128 1707
Size: 212 89 827 579
Fragments arranged by size:
827 579 212 89
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 399 417 1707
Size: 171 228 18 1290
Fragments arranged by size:
1290 228 171 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 813 849 1485 1707
Size: 46 767 36 636 222
Fragments arranged by size:
767 636 222 46 36
Bpu10I CC'TnA_GC
Cuts at: 0 973 1707
Size: 973 734
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 1707
Size: 70 1637
BsaAI yAC'GTr
Cuts at: 0 575 1707
Size: 575 1132
BsaBI GATnn'nnATC
Cuts at: 0 755 1707
Size: 755 952
BsaHI Gr'CG_yC
Cuts at: 0 152 539 586 1244 1707
Size: 152 387 47 658 463
Fragments arranged by size:
658 463 387 152 47
BsaWI w'CCGG_w
Cuts at: 0 1170 1707
Size: 1170 537
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 595 625 847 877 1707
Size: 106 30 459 30 222 30 830
Fragments arranged by size:
830 459 222 106 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 670 1209 1412 1467 1470 1485 1488
Size: 334 336 539 203 55 3 15 3
Cuts at: 1488 1707
Size: 219
Fragments arranged by size:
539 336 334 219 203 55 15 3
3
BseYI C'CCAG_C
Cuts at: 0 4 129 589 808 935 1431 1707
Size: 4 125 460 219 127 496 276
Fragments arranged by size:
496 460 276 219 127 125 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 1707
Size: 1279 428
BsiEI CG_ry'CG
Cuts at: 0 831 1707
Size: 831 876
BsiHKAI G_wGCw'C
Cuts at: 0 398 704 1286 1361 1707
Size: 398 306 582 75 346
Fragments arranged by size:
582 398 346 306 75
Bsp1286I G_dGCh'C
Cuts at: 0 99 398 521 533 704 1119 1134 1286
Size: 99 299 123 12 171 415 15 152
Cuts at: 1286 1361 1440 1477 1595 1655 1663 1707
Size: 75 79 37 118 60 8 44
Fragments arranged by size:
415 299 171 152 123 118 99 79
75 60 44 37 15 12 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 1170 1707
Size: 476 694 537
Fragments arranged by size:
694 537 476
BsrBI CCG'CTC
Cuts at: 0 1102 1143 1707
Size: 1102 41 564
Fragments arranged by size:
1102 564 41
BsrDI GCAATG_nn'
Cuts at: 0 671 1017 1707
Size: 671 346 690
Fragments arranged by size:
690 671 346
BsrFI r'CCGG_y
Cuts at: 0 606 622 685 1289 1707
Size: 606 16 63 604 418
Fragments arranged by size:
606 604 418 63 16
BssSI C'ACGA_G
Cuts at: 0 520 1707
Size: 520 1187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1310 1622 1707
Size: 1310 312 85
Fragments arranged by size:
1310 312 85
BstEII G'GTnAC_C
Cuts at: 0 618 1707
Size: 618 1089
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1127 1431 1707
Size: 1127 304 276
Fragments arranged by size:
1127 304 276
BtgI C'CryG_G
Cuts at: 0 362 1223 1676 1707
Size: 362 861 453 31
Fragments arranged by size:
861 453 362 31
BtsI GCAGTG_nn'
Cuts at: 0 885 1707
Size: 885 822
EaeI y'GGCC_r
Cuts at: 0 359 828 1599 1707
Size: 359 469 771 108
Fragments arranged by size:
771 469 359 108
EagI C'GGCC_G
Cuts at: 0 828 1707
Size: 828 879
EarI CTCTTCn'nnn_
Cuts at: 0 178 1194 1488 1512 1521 1615 1639 1707
Size: 178 1016 294 24 9 94 24 68
Fragments arranged by size:
1016 294 178 94 68 24 24 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 961 1172 1707
Size: 961 211 535
Fragments arranged by size:
961 535 211
EcoICRI GAG'CTC
Cuts at: 0 702 1707
Size: 702 1005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 813 849 1485 1707
Size: 46 767 36 636 222
Fragments arranged by size:
767 636 222 46 36
EcoO109I rG'GnC_Cy
Cuts at: 0 529 1707
Size: 529 1178
FspI TGC'GCA
Cuts at: 0 983 1381 1707
Size: 983 398 326
Fragments arranged by size:
983 398 326
HaeII r_GCGC'y
Cuts at: 0 155 274 542 942 1247 1707
Size: 155 119 268 400 305 460
Fragments arranged by size:
460 400 305 268 155 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1707
Size: 106 32 1569
Fragments arranged by size:
1569 106 32
KasI G'GCGC_C
Cuts at: 0 151 538 1243 1707
Size: 151 387 705 464
Fragments arranged by size:
705 464 387 151
KpnI G_GTAC'C
Cuts at: 0 502 1707
Size: 502 1205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1283 1442 1707
Size: 1283 159 265
Fragments arranged by size:
1283 265 159
MscI TGG'CCA
Cuts at: 0 361 1601 1707
Size: 361 1240 106
Fragments arranged by size:
1240 361 106
MslI CAynn'nnrTG
Cuts at: 0 490 739 1707
Size: 490 249 968
Fragments arranged by size:
968 490 249
MspA1I CmG'CkG
Cuts at: 0 41 65 928 1030 1310 1443 1678 1707
Size: 41 24 863 102 280 133 235 29
Fragments arranged by size:
863 280 235 133 102 41 29 24
NaeI GCC'GGC
Cuts at: 0 1291 1707
Size: 1291 416
NarI GG'CG_CC
Cuts at: 0 152 539 1244 1707
Size: 152 387 705 463
Fragments arranged by size:
705 463 387 152
NcoI C'CATG_G
Cuts at: 0 362 1707
Size: 362 1345
NgoMIV G'CCGG_C
Cuts at: 0 1289 1707
Size: 1289 418
NspI r_CATG'y
Cuts at: 0 232 630 1707
Size: 232 398 1077
Fragments arranged by size:
1077 398 232
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 1707
Size: 942 765
PfoI T'CCnGG_A
Cuts at: 0 418 1707
Size: 418 1289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1707
Size: 595 32 1080
Fragments arranged by size:
1080 595 32
PspOMI G'GGCC_C
Cuts at: 0 529 1130 1707
Size: 529 601 577
Fragments arranged by size:
601 577 529
PstI C_TGCA'G
Cuts at: 0 104 1079 1167 1707
Size: 104 975 88 540
Fragments arranged by size:
975 540 104 88
PvuII CAG'CTG
Cuts at: 0 1310 1443 1707
Size: 1310 133 264
Fragments arranged by size:
1310 264 133
SacI G_AGCT'C
Cuts at: 0 704 1707
Size: 704 1003
SacII CC_GC'GG
Cuts at: 0 1679 1707
Size: 1679 28
SapI GCTCTTCn'nnn_
Cuts at: 0 178 1194 1512 1639 1707
Size: 178 1016 318 127 68
Fragments arranged by size:
1016 318 178 127 68
SfcI C'TryA_G
Cuts at: 0 100 951 1075 1163 1707
Size: 100 851 124 88 544
Fragments arranged by size:
851 544 124 100 88
SfoI GGC'GCC
Cuts at: 0 153 540 1245 1707
Size: 153 387 705 462
Fragments arranged by size:
705 462 387 153
SmaI CCC'GGG
Cuts at: 0 474 1151 1236 1346 1391 1707
Size: 474 677 85 110 45 316
Fragments arranged by size:
677 474 316 110 85 45
SmlI C'TyrA_G
Cuts at: 0 650 1707
Size: 650 1057
SphI G_CATG'C
Cuts at: 0 630 1707
Size: 630 1077
SrfI GCCC'GGGC
Cuts at: 0 474 1707
Size: 474 1233
StuI AGG'CCT
Cuts at: 0 1403 1707
Size: 1403 304
StyI C'CwwG_G
Cuts at: 0 362 1707
Size: 362 1345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 1707
Size: 214 1493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 788 1266 1707
Size: 788 478 441
Fragments arranged by size:
788 478 441
XhoI C'TCGA_G
Cuts at: 0 650 1707
Size: 650 1057
XmaI C'CCGG_G
Cuts at: 0 472 1149 1234 1344 1389 1707
Size: 472 677 85 110 45 318
Fragments arranged by size:
677 472 318 110 85 45
XmnI GAAnn'nnTTC
Cuts at: 0 718 1707
Size: 718 989
Enzymes that do cut:
Acc65I AfeI AflIII AleI AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PfoI PpiI PspOMI PstI PvuII SacI SacII
SapI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BglII BlpI BmtI BpuEI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI TstI Tth111I XbaI ZraI