Restriction map for
pF1KA2010
(Linear) (Six-base) MAPSORT of: KIAA2010.seq Check: 8047 from: 1 to: 2460
KIAA2010.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AatII G_ACGT'C
Cuts at: 0 659 2460
Size: 659 1801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 239 371 659 2460
Size: 124 115 132 288 1801
Fragments arranged by size:
1801 288 132 124 115
AflIII A'CryG_T
Cuts at: 0 851 2460
Size: 851 1609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 2460
Size: 133 2327
AlwNI CAG_nnn'CTG
Cuts at: 0 763 1279 2460
Size: 763 516 1181
Fragments arranged by size:
1181 763 516
ApoI r'AATT_y
Cuts at: 0 275 712 779 925 1009 1375 1777 2104
Size: 275 437 67 146 84 366 402 327
Cuts at: 2104 2449 2460
Size: 345 11
Fragments arranged by size:
437 402 366 345 327 275 146 84
67 11
AseI AT'TA_AT
Cuts at: 0 1556 2460
Size: 1556 904
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1112 1145 1177 1210 2460
Size: 1112 33 32 33 1250
Fragments arranged by size:
1250 1112 33 33 32
BamHI G'GATC_C
Cuts at: 0 1732 2091 2278 2305 2460
Size: 1732 359 187 27 155
Fragments arranged by size:
1732 359 187 155 27
BanI G'GyrC_C
Cuts at: 0 67 2460
Size: 67 2393
BanII G_rGCy'C
Cuts at: 0 2185 2460
Size: 2185 275
BbsI GAAGACnn'nnnn_
Cuts at: 0 1451 2224 2460
Size: 1451 773 236
Fragments arranged by size:
1451 773 236
BfrBI ATG'CAT
Cuts at: 0 1407 2460
Size: 1407 1053
Bme1580I G_kGCm'C
Cuts at: 0 70 1626 2460
Size: 70 1556 834
Fragments arranged by size:
1556 834 70
BmtI G_CTAG'C
Cuts at: 0 1348 2460
Size: 1348 1112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 2276 2460
Size: 541 1735 184
Fragments arranged by size:
1735 541 184
Bpu10I CC'TnA_GC
Cuts at: 0 244 764 2460
Size: 244 520 1696
Fragments arranged by size:
1696 520 244
BsaAI yAC'GTr
Cuts at: 0 93 2460
Size: 93 2367
BsaBI GATnn'nnATC
Cuts at: 0 1274 2460
Size: 1274 1186
BsaHI Gr'CG_yC
Cuts at: 0 656 2460
Size: 656 1804
BsaWI w'CCGG_w
Cuts at: 0 1571 2460
Size: 1571 889
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1280 1310 2030 2060 2345 2375 2460
Size: 1280 30 720 30 285 30 85
Fragments arranged by size:
1280 720 285 85 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 1304 2460
Size: 370 934 1156
Fragments arranged by size:
1156 934 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 2460
Size: 550 1910
BsiEI CG_ry'CG
Cuts at: 0 63 2460
Size: 63 2397
BsiHKAI G_wGCw'C
Cuts at: 0 1526 2460
Size: 1526 934
Bsp1286I G_dGCh'C
Cuts at: 0 70 1526 1626 2185 2460
Size: 70 1456 100 559 275
Fragments arranged by size:
1456 559 275 100 70
BspEI T'CCGG_A
Cuts at: 0 1571 2460
Size: 1571 889
BsrBI CCG'CTC
Cuts at: 0 99 2460
Size: 99 2361
BsrFI r'CCGG_y
Cuts at: 0 47 2460
Size: 47 2413
BsrGI T'GTAC_A
Cuts at: 0 29 1860 2460
Size: 29 1831 600
Fragments arranged by size:
1831 600 29
BssSI C'ACGA_G
Cuts at: 0 2359 2460
Size: 2359 101
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1404 1621 2460
Size: 1404 217 839
Fragments arranged by size:
1404 839 217
BstBI TT'CG_AA
Cuts at: 0 1317 2460
Size: 1317 1143
BstYI r'GATC_y
Cuts at: 0 759 1275 1732 2091 2278 2287 2305 2460
Size: 759 516 457 359 187 9 18 155
Fragments arranged by size:
759 516 457 359 187 155 18 9
BtgI C'CryG_G
Cuts at: 0 317 1181 2460
Size: 317 864 1279
Fragments arranged by size:
1279 864 317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 362 2460
Size: 362 2098
BtsI GCAGTG_nn'
Cuts at: 0 58 176 1349 1415 2460
Size: 58 118 1173 66 1045
Fragments arranged by size:
1173 1045 118 66 58
DraI TTT'AAA
Cuts at: 0 738 1004 1635 1872 2460
Size: 738 266 631 237 588
Fragments arranged by size:
738 631 588 266 237
EarI CTCTTCn'nnn_
Cuts at: 0 347 467 1962 1991 2460
Size: 347 120 1495 29 469
Fragments arranged by size:
1495 469 347 120 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 239 371 541 659 2276 2460
Size: 124 115 132 170 118 1617 184
Fragments arranged by size:
1617 184 170 132 124 118 115
EcoO109I rG'GnC_Cy
Cuts at: 0 309 333 2460
Size: 309 24 2127
Fragments arranged by size:
2127 309 24
EcoRV GAT'ATC
Cuts at: 0 1566 1963 2460
Size: 1566 397 497
Fragments arranged by size:
1566 497 397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1123 2460
Size: 1123 1337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 361 390 393 1138 1170 1181 1213
Size: 358 3 29 3 745 32 11 32
Cuts at: 1213 1280 1312 1786 1818 2460
Size: 67 32 474 32 642
Fragments arranged by size:
745 642 474 358 67 32 32 32
32 29 11 3 3
HincII GTy'rAC
Cuts at: 0 996 1329 1508 2460
Size: 996 333 179 952
Fragments arranged by size:
996 952 333 179
HindIII A'AGCT_T
Cuts at: 0 2194 2460
Size: 2194 266
HpaI GTT'AAC
Cuts at: 0 996 1508 2460
Size: 996 512 952
Fragments arranged by size:
996 952 512
MslI CAynn'nnrTG
Cuts at: 0 536 575 2460
Size: 536 39 1885
Fragments arranged by size:
1885 536 39
NcoI C'CATG_G
Cuts at: 0 1181 2460
Size: 1181 1279
NdeI CA'TA_TG
Cuts at: 0 1259 2460
Size: 1259 1201
NheI G'CTAG_C
Cuts at: 0 1344 2460
Size: 1344 1116
NsiI A_TGCA'T
Cuts at: 0 1409 2460
Size: 1409 1051
NspI r_CATG'y
Cuts at: 0 79 114 855 909 1344 1411 1606 2460
Size: 79 35 741 54 435 67 195 854
Fragments arranged by size:
854 741 435 195 79 67 54 35
PciI A'CATG_T
Cuts at: 0 851 2460
Size: 851 1609
PflMI CCAn_nnn'nTGG
Cuts at: 0 1305 1485 2460
Size: 1305 180 975
Fragments arranged by size:
1305 975 180
PfoI T'CCnGG_A
Cuts at: 0 518 2265 2301 2460
Size: 518 1747 36 159
Fragments arranged by size:
1747 518 159 36
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 1568 1600 2460
Size: 126 32 1410 32 860
Fragments arranged by size:
1410 860 126 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 309 333 2460
Size: 309 24 2127
Fragments arranged by size:
2127 309 24
SfcI C'TryA_G
Cuts at: 0 1033 2045 2460
Size: 1033 1012 415
Fragments arranged by size:
1033 1012 415
SphI G_CATG'C
Cuts at: 0 1606 2460
Size: 1606 854
SspI AAT'ATT
Cuts at: 0 555 2460
Size: 555 1905
StuI AGG'CCT
Cuts at: 0 2357 2460
Size: 2357 103
StyI C'CwwG_G
Cuts at: 0 1107 1181 2460
Size: 1107 74 1279
Fragments arranged by size:
1279 1107 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 825 2460
Size: 19 806 1635
Fragments arranged by size:
1635 806 19
TatI w'GTAC_w
Cuts at: 0 29 1860 2460
Size: 29 1831 600
Fragments arranged by size:
1831 600 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 2345 2377 2460
Size: 37 32 2276 32 83
Fragments arranged by size:
2276 83 37 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1068 2460
Size: 1068 1392
XmnI GAAnn'nnTTC
Cuts at: 0 746 926 2150 2460
Size: 746 180 1224 310
Fragments arranged by size:
1224 746 310 180
ZraI GAC'GTC
Cuts at: 0 657 2460
Size: 657 1803
Enzymes that do cut:
AatII AcuI AflIII AlfI AlwNI ApoI AseI BaeI
BamHI BanI BanII BbsI BfrBI Bme1580I BmtI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI EarI
Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII HpaI
MslI NcoI NdeI NheI NsiI NspI PciI PflMI
PfoI PpiI PpuMI SfcI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmgBI BmrI BplI BpuEI BsaI BseYI BsiWI BsmI
BsmBI BspHI BspMI BsrDI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NgoMIV NotI NruI PacI PasI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SwaI XbaI XcmI XhoI XmaI