Restriction map for
pF1KA2014
(Linear) (Six-base) MAPSORT of: KIAA2014.seq Check: 8569 from: 1 to: 3081
KIAA2014.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1034 1092 3081
Size: 1034 58 1989
Fragments arranged by size:
1989 1034 58
AatII G_ACGT'C
Cuts at: 0 671 3081
Size: 671 2410
Acc65I G'GTAC_C
Cuts at: 0 2971 3081
Size: 2971 110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1749 2133 2308 3081
Size: 655 1094 384 175 773
Fragments arranged by size:
1094 773 655 384 175
AflIII A'CryG_T
Cuts at: 0 1130 3081
Size: 1130 1951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 913 1865 1910 2258 3081
Size: 913 952 45 348 823
Fragments arranged by size:
952 913 823 348 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 3081
Size: 94 32 2955
Fragments arranged by size:
2955 94 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2065 2109 2286 2376 3081
Size: 2065 44 177 90 705
Fragments arranged by size:
2065 705 177 90 44
ApaI G_GGCC'C
Cuts at: 0 633 1849 3081
Size: 633 1216 1232
Fragments arranged by size:
1232 1216 633
ApaLI G'TGCA_C
Cuts at: 0 991 1351 3081
Size: 991 360 1730
Fragments arranged by size:
1730 991 360
ApoI r'AATT_y
Cuts at: 0 379 3081
Size: 379 2702
AvaI C'yCGr_G
Cuts at: 0 30 3062 3081
Size: 30 3032 19
Fragments arranged by size:
3032 30 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 663 696 3081
Size: 663 33 2385
Fragments arranged by size:
2385 663 33
BanI G'GyrC_C
Cuts at: 0 2927 2971 3081
Size: 2927 44 110
Fragments arranged by size:
2927 110 44
BanII G_rGCy'C
Cuts at: 0 41 92 130 633 1849 3069 3081
Size: 41 51 38 503 1216 1220 12
Fragments arranged by size:
1220 1216 503 51 41 38 12
BbeI G_GCGC'C
Cuts at: 0 2931 3081
Size: 2931 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 1710 1755 2267 3081
Size: 1710 45 512 814
Fragments arranged by size:
1710 814 512 45
BbvCI CC'TCA_GC
Cuts at: 0 1973 2066 2126 2672 3081
Size: 1973 93 60 546 409
Fragments arranged by size:
1973 546 409 93 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 504 538 3081
Size: 504 34 2543
Fragments arranged by size:
2543 504 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 691 2385 3081
Size: 691 1694 696
Fragments arranged by size:
1694 696 691
BglI GCCn_nnn'nGGC
Cuts at: 0 553 2668 3081
Size: 553 2115 413
Fragments arranged by size:
2115 553 413
BglII A'GATC_T
Cuts at: 0 341 1787 1979 3081
Size: 341 1446 192 1102
Fragments arranged by size:
1446 1102 341 192
BlpI GC'TnA_GC
Cuts at: 0 124 3081
Size: 124 2957
Bme1580I G_kGCm'C
Cuts at: 0 633 995 1355 1849 3081
Size: 633 362 360 494 1232
Fragments arranged by size:
1232 633 494 362 360
BmgBI CAC'GTC
Cuts at: 0 675 2541 3081
Size: 675 1866 540
Fragments arranged by size:
1866 675 540
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 432 2326 2740 3044 3081
Size: 269 163 1894 414 304 37
Fragments arranged by size:
1894 414 304 269 163 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 3081
Size: 60 3021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 121 128 241 358 531 1204 1453
Size: 31 90 7 113 117 173 673 249
Cuts at: 1453 1505 2131 2353 2542 2635 3053 3081
Size: 52 626 222 189 93 418 28
Fragments arranged by size:
673 626 418 249 222 189 173 117
113 93 90 52 31 28 7
Bpu10I CC'TnA_GC
Cuts at: 0 86 1973 2066 2126 2672 2843 3081
Size: 86 1887 93 60 546 171 238
Fragments arranged by size:
1887 546 238 171 93 86 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 3081
Size: 1159 1922
BsaHI Gr'CG_yC
Cuts at: 0 668 2257 2660 2928 3081
Size: 668 1589 403 268 153
Fragments arranged by size:
1589 668 403 268 153
BsaWI w'CCGG_w
Cuts at: 0 503 602 929 2408 2609 3081
Size: 503 99 327 1479 201 472
Fragments arranged by size:
1479 503 472 327 201 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 1198 1228 2712 2742 3081
Size: 94 30 1074 30 1484 30 339
Fragments arranged by size:
1484 1074 339 94 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 315 854 1072 1210 1219 1399 1552 1990
Size: 315 539 218 138 9 180 153 438
Cuts at: 1990 2128 2689 2830 3081
Size: 138 561 141 251
Fragments arranged by size:
561 539 438 315 251 218 180 153
141 138 138 9
BseYI C'CCAG_C
Cuts at: 0 541 1047 1614 2171 2550 2879 3081
Size: 541 506 567 557 379 329 202
Fragments arranged by size:
567 557 541 506 379 329 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 883 1126 1622 2473 2604 3026 3081
Size: 328 555 243 496 851 131 422 55
Fragments arranged by size:
851 555 496 422 328 243 131 55
BsiHKAI G_wGCw'C
Cuts at: 0 130 602 995 1355 3081
Size: 130 472 393 360 1726
Fragments arranged by size:
1726 472 393 360 130
Bsp1286I G_dGCh'C
Cuts at: 0 41 92 130 602 633 995 1355 1849
Size: 41 51 38 472 31 362 360 494
Cuts at: 1849 3069 3081
Size: 1220 12
Fragments arranged by size:
1220 494 472 362 360 51 41 38
31 12
BspEI T'CCGG_A
Cuts at: 0 929 2609 3081
Size: 929 1680 472
Fragments arranged by size:
1680 929 472
BspHI T'CATG_A
Cuts at: 0 701 3081
Size: 701 2380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 506 539 1034 1092 2225 3081
Size: 146 360 33 495 58 1133 856
Fragments arranged by size:
1133 856 495 360 146 58 33
BsrBI CCG'CTC
Cuts at: 0 1967 2094 2100 3081
Size: 1967 127 6 981
Fragments arranged by size:
1967 981 127 6
BsrDI GCAATG_nn'
Cuts at: 0 2249 3081
Size: 2249 832
BsrFI r'CCGG_y
Cuts at: 0 2945 3081
Size: 2945 136
BssHII G'CGCG_C
Cuts at: 0 586 3081
Size: 586 2495
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1359 2487 3081
Size: 1359 1128 594
Fragments arranged by size:
1359 1128 594
BstBI TT'CG_AA
Cuts at: 0 1677 3081
Size: 1677 1404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1753 1938 2859 3081
Size: 1753 185 921 222
Fragments arranged by size:
1753 921 222 185
BstYI r'GATC_y
Cuts at: 0 192 341 1787 1803 1979 2379 3081
Size: 192 149 1446 16 176 400 702
Fragments arranged by size:
1446 702 400 192 176 149 16
Bsu36I CC'TnA_GG
Cuts at: 0 1535 3081
Size: 1535 1546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2418 3081
Size: 2418 663
BtsI GCAGTG_nn'
Cuts at: 0 2419 2519 3000 3081
Size: 2419 100 481 81
Fragments arranged by size:
2419 481 100 81
DraIII CAC_nnn'GTG
Cuts at: 0 999 1350 3081
Size: 999 351 1731
Fragments arranged by size:
1731 999 351
DrdI GACnn_nn'nnGTC
Cuts at: 0 673 2602 3081
Size: 673 1929 479
Fragments arranged by size:
1929 673 479
EaeI y'GGCC_r
Cuts at: 0 1457 1940 1962 3081
Size: 1457 483 22 1119
Fragments arranged by size:
1457 1119 483 22
EarI CTCTTCn'nnn_
Cuts at: 0 353 1556 2152 3081
Size: 353 1203 596 929
Fragments arranged by size:
1203 929 596 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1559 3081
Size: 1559 1522
EcoICRI GAG'CTC
Cuts at: 0 128 3081
Size: 128 2953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 121 128 241 358 531 655 1204
Size: 31 90 7 113 117 173 124 549
Cuts at: 1204 1453 1505 1749 2131 2133 2308 2353 2542
Size: 249 52 244 382 2 175 45 189
Cuts at: 2542 2635 3053 3081
Size: 93 418 28
Fragments arranged by size:
549 418 382 249 244 189 175 173
124 117 113 93 90 52 45 31
28 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 513 1186 1954 3081
Size: 513 673 768 1127
Fragments arranged by size:
1127 768 673 513
EcoO109I rG'GnC_Cy
Cuts at: 0 27 528 629 630 1428 1452 1468 1542
Size: 27 501 101 1 798 24 16 74
Cuts at: 1542 1797 1845 1846 3073 3081
Size: 255 48 1 1227 8
Fragments arranged by size:
1227 798 501 255 101 74 48 27
24 16 8 1 1
HaeII r_GCGC'y
Cuts at: 0 20 548 2931 3081
Size: 20 528 2383 150
Fragments arranged by size:
2383 528 150 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 1198 1230 2364 2383 2396 2415
Size: 412 32 754 32 1134 19 13 19
Cuts at: 2415 2712 2744 3081
Size: 297 32 337
Fragments arranged by size:
1134 754 412 337 297 32 32 32
19 19 13
HincII GTy'rAC
Cuts at: 0 317 1655 2168 3081
Size: 317 1338 513 913
Fragments arranged by size:
1338 913 513 317
HindIII A'AGCT_T
Cuts at: 0 1231 3081
Size: 1231 1850
KasI G'GCGC_C
Cuts at: 0 2927 3081
Size: 2927 154
KpnI G_GTAC'C
Cuts at: 0 2975 3081
Size: 2975 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 1181 1526 1895 2279 3081
Size: 1133 48 345 369 384 802
Fragments arranged by size:
1133 802 384 369 345 48
MscI TGG'CCA
Cuts at: 0 1459 1942 3081
Size: 1459 483 1139
Fragments arranged by size:
1459 1139 483
MslI CAynn'nnrTG
Cuts at: 0 743 988 3081
Size: 743 245 2093
Fragments arranged by size:
2093 743 245
MspA1I CmG'CkG
Cuts at: 0 823 1287 1296 2125 2655 2838 2883 3081
Size: 823 464 9 829 530 183 45 198
Fragments arranged by size:
829 823 530 464 198 183 45 9
NarI GG'CG_CC
Cuts at: 0 2928 3081
Size: 2928 153
NdeI CA'TA_TG
Cuts at: 0 1329 3081
Size: 1329 1752
NspI r_CATG'y
Cuts at: 0 972 1213 1494 3081
Size: 972 241 281 1587
Fragments arranged by size:
1587 972 281 241
PasI CC'CwG_GG
Cuts at: 0 619 3081
Size: 619 2462
PflMI CCAn_nnn'nTGG
Cuts at: 0 2178 2376 3012 3081
Size: 2178 198 636 69
Fragments arranged by size:
2178 636 198 69
PfoI T'CCnGG_A
Cuts at: 0 508 2207 3081
Size: 508 1699 874
Fragments arranged by size:
1699 874 508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 3081
Size: 94 32 2955
Fragments arranged by size:
2955 94 32
PpuMI rG'GwC_Cy
Cuts at: 0 27 528 1542 1797 3081
Size: 27 501 1014 255 1284
Fragments arranged by size:
1284 1014 501 255 27
PshAI GACnn'nnGTC
Cuts at: 0 1607 3081
Size: 1607 1474
PspOMI G'GGCC_C
Cuts at: 0 629 1845 3081
Size: 629 1216 1236
Fragments arranged by size:
1236 1216 629
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 697 729 3081
Size: 697 32 2352
Fragments arranged by size:
2352 697 32
PstI C_TGCA'G
Cuts at: 0 536 1031 1070 1103 1476 1987 2222 2511
Size: 536 495 39 33 373 511 235 289
Cuts at: 2511 3081
Size: 570
Fragments arranged by size:
570 536 511 495 373 289 235 39
33
PvuII CAG'CTG
Cuts at: 0 823 1287 2125 2838 3081
Size: 823 464 838 713 243
Fragments arranged by size:
838 823 713 464 243
SacI G_AGCT'C
Cuts at: 0 130 3081
Size: 130 2951
SanDI GG'GwC_CC
Cuts at: 0 27 3081
Size: 27 3054
SapI GCTCTTCn'nnn_
Cuts at: 0 2152 3081
Size: 2152 929
ScaI AGT'ACT
Cuts at: 0 326 3081
Size: 326 2755
SexAI A'CCwGG_T
Cuts at: 0 725 1346 3081
Size: 725 621 1735
Fragments arranged by size:
1735 725 621
SfcI C'TryA_G
Cuts at: 0 532 1027 1066 1099 1291 1472 1983 2218
Size: 532 495 39 33 192 181 511 235
Cuts at: 2218 2507 3081
Size: 289 574
Fragments arranged by size:
574 532 511 495 289 235 192 181
39 33
SfoI GGC'GCC
Cuts at: 0 2929 3081
Size: 2929 152
SmaI CCC'GGG
Cuts at: 0 32 3081
Size: 32 3049
SphI G_CATG'C
Cuts at: 0 972 1494 3081
Size: 972 522 1587
Fragments arranged by size:
1587 972 522
SspI AAT'ATT
Cuts at: 0 948 3081
Size: 948 2133
StuI AGG'CCT
Cuts at: 0 404 1390 2680 2996 3081
Size: 404 986 1290 316 85
Fragments arranged by size:
1290 986 404 316 85
StyI C'CwwG_G
Cuts at: 0 787 2936 3081
Size: 787 2149 145
Fragments arranged by size:
2149 787 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 356 360 555 3081
Size: 356 4 195 2526
Fragments arranged by size:
2526 356 195 4
TatI w'GTAC_w
Cuts at: 0 324 411 3081
Size: 324 87 2670
Fragments arranged by size:
2670 324 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2179 3081
Size: 2179 902
XmaI C'CCGG_G
Cuts at: 0 30 3081
Size: 30 3051
XmnI GAAnn'nnTTC
Cuts at: 0 292 3081
Size: 292 2789
ZraI GAC'GTC
Cuts at: 0 669 3081
Size: 669 2412
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BaeI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NarI
NdeI NspI PasI PflMI PfoI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuII SacI SanDI SapI ScaI
SexAI SfcI SfoI SmaI SphI SspI StuI StyI
TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI AscI
AseI AsiSI AvrII BamHI BclI BfrBI BmtI BpuEI
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BsrGI BssSI
BstEII BstZ17I BtgI ClaI CspCI DraI EagI EcoRI
EcoRV FalI FseI FspI FspAI HpaI MfeI MluI
NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PmlI PsiI PspXI PvuI RsrII SacII
SalI SbfI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SwaI TstI Tth111I XbaI XhoI