Restriction map for
pF1KA2027
(Linear) (Six-base) MAPSORT of: KIAA2027.seq Check: 6926 from: 1 to: 2328
KIAA2027.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 279 409 2328
Size: 279 130 1919
Fragments arranged by size:
1919 279 130
AatII G_ACGT'C
Cuts at: 0 2051 2328
Size: 2051 277
AccI GT'mk_AC
Cuts at: 0 1201 1651 2328
Size: 1201 450 677
Fragments arranged by size:
1201 677 450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 677 1051 2328
Size: 473 204 374 1277
Fragments arranged by size:
1277 473 374 204
AflIII A'CryG_T
Cuts at: 0 1297 2328
Size: 1297 1031
AhdI GACnn_n'nnGTC
Cuts at: 0 2106 2328
Size: 2106 222
AleI CACnn'nnGTG
Cuts at: 0 1091 2328
Size: 1091 1237
AlwNI CAG_nnn'CTG
Cuts at: 0 1855 1954 1957 2328
Size: 1855 99 3 371
Fragments arranged by size:
1855 371 99 3
ApaI G_GGCC'C
Cuts at: 0 769 2328
Size: 769 1559
ApaLI G'TGCA_C
Cuts at: 0 1174 2328
Size: 1174 1154
ApoI r'AATT_y
Cuts at: 0 1459 2328
Size: 1459 869
AseI AT'TA_AT
Cuts at: 0 2167 2328
Size: 2167 161
AvaI C'yCGr_G
Cuts at: 0 336 762 2328
Size: 336 426 1566
Fragments arranged by size:
1566 426 336
BamHI G'GATC_C
Cuts at: 0 1581 2328
Size: 1581 747
BanI G'GyrC_C
Cuts at: 0 145 849 2328
Size: 145 704 1479
Fragments arranged by size:
1479 704 145
BanII G_rGCy'C
Cuts at: 0 769 1115 2328
Size: 769 346 1213
Fragments arranged by size:
1213 769 346
BbsI GAAGACnn'nnnn_
Cuts at: 0 71 227 1870 2306 2328
Size: 71 156 1643 436 22
Fragments arranged by size:
1643 436 156 71 22
BbvCI CC'TCA_GC
Cuts at: 0 166 2328
Size: 166 2162
BciVI GTATCCnnnnn_n'
Cuts at: 0 1024 2328
Size: 1024 1304
BclI T'GATC_A
Cuts at: 0 420 1563 2328
Size: 420 1143 765
Fragments arranged by size:
1143 765 420
BglI GCCn_nnn'nGGC
Cuts at: 0 773 2328
Size: 773 1555
Bme1580I G_kGCm'C
Cuts at: 0 150 769 1156 1178 2328
Size: 150 619 387 22 1150
Fragments arranged by size:
1150 619 387 150 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1004 2328
Size: 1004 1324
BmtI G_CTAG'C
Cuts at: 0 1132 2328
Size: 1132 1196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 547 2328
Size: 547 1781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 587 1129 1263 1316 1827 1961 2002
Size: 557 30 542 134 53 511 134 41
Cuts at: 2002 2328
Size: 326
Fragments arranged by size:
557 542 511 326 134 134 53 41
30
Bpu10I CC'TnA_GC
Cuts at: 0 166 1049 1813 1961 2328
Size: 166 883 764 148 367
Fragments arranged by size:
883 764 367 166 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1909 2328
Size: 1909 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 1281 2328
Size: 208 1073 1047
Fragments arranged by size:
1073 1047 208
BsaAI yAC'GTr
Cuts at: 0 1298 2328
Size: 1298 1030
BsaBI GATnn'nnATC
Cuts at: 0 1414 2328
Size: 1414 914
BsaHI Gr'CG_yC
Cuts at: 0 527 2048 2283 2328
Size: 527 1521 235 45
Fragments arranged by size:
1521 527 235 45
BsaWI w'CCGG_w
Cuts at: 0 55 2328
Size: 55 2273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 1921 1951 2328
Size: 98 30 1793 30 377
Fragments arranged by size:
1793 377 98 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1616 2328
Size: 1616 712
BseYI C'CCAG_C
Cuts at: 0 299 937 1289 1903 2328
Size: 299 638 352 614 425
Fragments arranged by size:
638 614 425 352 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 616 985 2328
Size: 280 336 369 1343
Fragments arranged by size:
1343 369 336 280
BsiHKAI G_wGCw'C
Cuts at: 0 1047 1115 1178 1774 2328
Size: 1047 68 63 596 554
Fragments arranged by size:
1047 596 554 68 63
BsmI GAATG_Cn'
Cuts at: 0 132 2328
Size: 132 2196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1002 2328
Size: 1002 1326
Bsp1286I G_dGCh'C
Cuts at: 0 150 769 1047 1115 1156 1178 1774 2328
Size: 150 619 278 68 41 22 596 554
Fragments arranged by size:
619 596 554 278 150 68 41 22
BspEI T'CCGG_A
Cuts at: 0 55 2328
Size: 55 2273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 279 409 1357 2328
Size: 189 90 130 948 971
Fragments arranged by size:
971 948 189 130 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1957 2328
Size: 1957 371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1093 1156 1711 2328
Size: 1093 63 555 617
Fragments arranged by size:
1093 617 555 63
BstYI r'GATC_y
Cuts at: 0 357 991 1581 2197 2328
Size: 357 634 590 616 131
Fragments arranged by size:
634 616 590 357 131
Bsu36I CC'TnA_GG
Cuts at: 0 1628 2328
Size: 1628 700
BtgI C'CryG_G
Cuts at: 0 1832 2328
Size: 1832 496
BtsI GCAGTG_nn'
Cuts at: 0 755 977 1019 1098 2328
Size: 755 222 42 79 1230
Fragments arranged by size:
1230 755 222 79 42
EaeI y'GGCC_r
Cuts at: 0 1503 2328
Size: 1503 825
EarI CTCTTCn'nnn_
Cuts at: 0 1337 2328
Size: 1337 991
EcoICRI GAG'CTC
Cuts at: 0 1113 2328
Size: 1113 1215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 557 587 677 1051 1129 1263 1316
Size: 473 84 30 90 374 78 134 53
Cuts at: 1316 1827 1961 2002 2328
Size: 511 134 41 326
Fragments arranged by size:
511 473 374 326 134 134 90 84
78 53 41 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 521 2328
Size: 196 325 1807
Fragments arranged by size:
1807 325 196
EcoO109I rG'GnC_Cy
Cuts at: 0 698 766 1248 2328
Size: 698 68 482 1080
Fragments arranged by size:
1080 698 482 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 538 570 976 1008 1566 1598
Size: 98 32 408 32 406 32 558 32
Cuts at: 1598 1857 1889 2092 2124 2328
Size: 259 32 203 32 204
Fragments arranged by size:
558 408 406 259 204 203 98 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1349 2328
Size: 1349 979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1236 2304 2328
Size: 1236 1068 24
Fragments arranged by size:
1236 1068 24
MslI CAynn'nnrTG
Cuts at: 0 1091 2328
Size: 1091 1237
MspA1I CmG'CkG
Cuts at: 0 181 303 855 1071 1189 1195 1729 1954
Size: 181 122 552 216 118 6 534 225
Cuts at: 1954 2022 2328
Size: 68 306
Fragments arranged by size:
552 534 306 225 216 181 122 118
68 6
NcoI C'CATG_G
Cuts at: 0 1832 2328
Size: 1832 496
NheI G'CTAG_C
Cuts at: 0 1128 2328
Size: 1128 1200
NruI TCG'CGA
Cuts at: 0 2122 2328
Size: 2122 206
NspI r_CATG'y
Cuts at: 0 830 1427 2328
Size: 830 597 901
Fragments arranged by size:
901 830 597
PasI CC'CwG_GG
Cuts at: 0 173 2112 2328
Size: 173 1939 216
Fragments arranged by size:
1939 216 173
PflMI CCAn_nnn'nTGG
Cuts at: 0 1107 1855 2328
Size: 1107 748 473
Fragments arranged by size:
1107 748 473
PfoI T'CCnGG_A
Cuts at: 0 1977 2328
Size: 1977 351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 2328
Size: 149 32 2147
Fragments arranged by size:
2147 149 32
PpuMI rG'GwC_Cy
Cuts at: 0 698 1248 2328
Size: 698 550 1080
Fragments arranged by size:
1080 698 550
PspOMI G'GGCC_C
Cuts at: 0 765 2328
Size: 765 1563
PstI C_TGCA'G
Cuts at: 0 156 348 1194 2273 2328
Size: 156 192 846 1079 55
Fragments arranged by size:
1079 846 192 156 55
PvuII CAG'CTG
Cuts at: 0 181 303 1071 1189 1195 1729 1954 2022
Size: 181 122 768 118 6 534 225 68
Cuts at: 2022 2328
Size: 306
Fragments arranged by size:
768 534 306 225 181 122 118 68
6
SacI G_AGCT'C
Cuts at: 0 1115 2328
Size: 1115 1213
SexAI A'CCwGG_T
Cuts at: 0 1239 2328
Size: 1239 1089
SfcI C'TryA_G
Cuts at: 0 152 344 644 1190 1202 2269 2328
Size: 152 192 300 546 12 1067 59
Fragments arranged by size:
1067 546 300 192 152 59 12
SmlI C'TyrA_G
Cuts at: 0 1924 2328
Size: 1924 404
SphI G_CATG'C
Cuts at: 0 1427 2328
Size: 1427 901
StyI C'CwwG_G
Cuts at: 0 771 1832 2096 2328
Size: 771 1061 264 232
Fragments arranged by size:
1061 771 264 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 1825 2328
Size: 235 1590 503
Fragments arranged by size:
1590 503 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2038 2070 2328
Size: 2038 32 258
Fragments arranged by size:
2038 258 32
ZraI GAC'GTC
Cuts at: 0 2049 2328
Size: 2049 279
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BciVI BclI BglI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BstAPI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I
HincII MmeI MslI MspA1I NcoI NheI NruI NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SexAI SfcI SmlI SphI StyI TaqII TstI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AlfI AloI AscI
AsiSI AvrII BaeI BbeI BcgI BfrBI BglII BlpI
BmgBI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI XmnI