Restriction map for
pF1KA2032
(Linear) (Six-base) MAPSORT of: KIAA2032.seq Check: 2146 from: 1 to: 2832
KIAA2032.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 840 2832
Size: 840 1992
Acc65I G'GTAC_C
Cuts at: 0 666 1762 2832
Size: 666 1096 1070
Fragments arranged by size:
1096 1070 666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1520 2243 2759 2832
Size: 1498 22 723 516 73
Fragments arranged by size:
1498 723 516 73 22
AleI CACnn'nnGTG
Cuts at: 0 1708 2832
Size: 1708 1124
AlwNI CAG_nnn'CTG
Cuts at: 0 898 2832
Size: 898 1934
ApoI r'AATT_y
Cuts at: 0 294 470 542 1260 2832
Size: 294 176 72 718 1572
Fragments arranged by size:
1572 718 294 176 72
AseI AT'TA_AT
Cuts at: 0 875 2173 2832
Size: 875 1298 659
Fragments arranged by size:
1298 875 659
AvaI C'yCGr_G
Cuts at: 0 2418 2619 2832
Size: 2418 201 213
Fragments arranged by size:
2418 213 201
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1746 1779 2832
Size: 1746 33 1053
Fragments arranged by size:
1746 1053 33
BanI G'GyrC_C
Cuts at: 0 666 1762 2430 2832
Size: 666 1096 668 402
Fragments arranged by size:
1096 668 666 402
BanII G_rGCy'C
Cuts at: 0 149 1295 1832 2832
Size: 149 1146 537 1000
Fragments arranged by size:
1146 1000 537 149
BbeI G_GCGC'C
Cuts at: 0 2434 2832
Size: 2434 398
BbsI GAAGACnn'nnnn_
Cuts at: 0 456 743 2832
Size: 456 287 2089
Fragments arranged by size:
2089 456 287
BbvCI CC'TCA_GC
Cuts at: 0 963 980 1731 2514 2832
Size: 963 17 751 783 318
Fragments arranged by size:
963 783 751 318 17
BciVI GTATCCnnnnn_n'
Cuts at: 0 85 2635 2832
Size: 85 2550 197
Fragments arranged by size:
2550 197 85
BclI T'GATC_A
Cuts at: 0 1817 2832
Size: 1817 1015
BglII A'GATC_T
Cuts at: 0 1770 2832
Size: 1770 1062
BlpI GC'TnA_GC
Cuts at: 0 2747 2832
Size: 2747 85
Bme1580I G_kGCm'C
Cuts at: 0 1946 2832
Size: 1946 886
BmgBI CAC'GTC
Cuts at: 0 1706 2832
Size: 1706 1126
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 856 2832
Size: 144 712 1976
Fragments arranged by size:
1976 712 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1580 1974 2477 2832
Size: 1580 394 503 355
Fragments arranged by size:
1580 503 394 355
Bpu10I CC'TnA_GC
Cuts at: 0 963 980 1731 2514 2832
Size: 963 17 751 783 318
Fragments arranged by size:
963 783 751 318 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2016 2315 2832
Size: 2016 299 517
Fragments arranged by size:
2016 517 299
BsaI GGTCTCn'nnnn_
Cuts at: 0 963 1990 2832
Size: 963 1027 842
Fragments arranged by size:
1027 963 842
BsaHI Gr'CG_yC
Cuts at: 0 2431 2832
Size: 2431 401
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1050 1080 1578 1608 1635 1665 2832
Size: 1050 30 498 30 27 30 1167
Fragments arranged by size:
1167 1050 498 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 797 951 1446 1719 2832
Size: 383 414 154 495 273 1113
Fragments arranged by size:
1113 495 414 383 273 154
BseYI C'CCAG_C
Cuts at: 0 786 1366 2487 2832
Size: 786 580 1121 345
Fragments arranged by size:
1121 786 580 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 1016 1184 2832
Size: 521 495 168 1648
Fragments arranged by size:
1648 521 495 168
BsmI GAATG_Cn'
Cuts at: 0 2518 2832
Size: 2518 314
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1631 2832
Size: 1631 1201
Bsp1286I G_dGCh'C
Cuts at: 0 149 1295 1832 1946 2832
Size: 149 1146 537 114 886
Fragments arranged by size:
1146 886 537 149 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 649 840 929 945 2700 2832
Size: 649 191 89 16 1755 132
Fragments arranged by size:
1755 649 191 132 89 16
BsrBI CCG'CTC
Cuts at: 0 2542 2832
Size: 2542 290
BsrDI GCAATG_nn'
Cuts at: 0 167 1021 1660 2066 2832
Size: 167 854 639 406 766
Fragments arranged by size:
854 766 639 406 167
BsrFI r'CCGG_y
Cuts at: 0 1375 2557 2832
Size: 1375 1182 275
Fragments arranged by size:
1375 1182 275
BsrGI T'GTAC_A
Cuts at: 0 1668 2832
Size: 1668 1164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 634 643 2832
Size: 634 9 2189
Fragments arranged by size:
2189 634 9
BstBI TT'CG_AA
Cuts at: 0 745 2832
Size: 745 2087
BstYI r'GATC_y
Cuts at: 0 1770 2832
Size: 1770 1062
BtgI C'CryG_G
Cuts at: 0 1156 1631 2832
Size: 1156 475 1201
Fragments arranged by size:
1201 1156 475
BtsI GCAGTG_nn'
Cuts at: 0 1028 1099 1685 2081 2300 2832
Size: 1028 71 586 396 219 532
Fragments arranged by size:
1028 586 532 396 219 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 188 223 2049 2084 2832
Size: 188 35 1826 35 748
Fragments arranged by size:
1826 748 188 35 35
DraI TTT'AAA
Cuts at: 0 2009 2832
Size: 2009 823
DraIII CAC_nnn'GTG
Cuts at: 0 643 1387 2832
Size: 643 744 1445
Fragments arranged by size:
1445 744 643
EarI CTCTTCn'nnn_
Cuts at: 0 1239 1517 1533 1811 2093 2411 2832
Size: 1239 278 16 278 282 318 421
Fragments arranged by size:
1239 421 318 282 278 278 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1520 1580 1974 2243 2477 2759 2832
Size: 1498 22 60 394 269 234 282 73
Fragments arranged by size:
1498 394 282 269 234 73 60 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1142 2832
Size: 1142 1690
EcoO109I rG'GnC_Cy
Cuts at: 0 1108 1147 2335 2832
Size: 1108 39 1188 497
Fragments arranged by size:
1188 1108 497 39
EcoRI G'AATT_C
Cuts at: 0 294 542 2832
Size: 294 248 2290
Fragments arranged by size:
2290 294 248
EcoRV GAT'ATC
Cuts at: 0 407 2832
Size: 407 2425
FspI TGC'GCA
Cuts at: 0 2690 2832
Size: 2690 142
HaeII r_GCGC'y
Cuts at: 0 2434 2782 2832
Size: 2434 348 50
Fragments arranged by size:
2434 348 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1578 1610 2832
Size: 27 1551 32 1222
Fragments arranged by size:
1551 1222 32 27
HincII GTy'rAC
Cuts at: 0 796 2832
Size: 796 2036
KasI G'GCGC_C
Cuts at: 0 2430 2832
Size: 2430 402
KpnI G_GTAC'C
Cuts at: 0 670 1766 2832
Size: 670 1096 1066
Fragments arranged by size:
1096 1066 670
MfeI C'AATT_G
Cuts at: 0 74 2832
Size: 74 2758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 2832
Size: 1355 1477
MslI CAynn'nnrTG
Cuts at: 0 1708 2832
Size: 1708 1124
MspA1I CmG'CkG
Cuts at: 0 898 2194 2236 2449 2832
Size: 898 1296 42 213 383
Fragments arranged by size:
1296 898 383 213 42
NarI GG'CG_CC
Cuts at: 0 2431 2832
Size: 2431 401
NspI r_CATG'y
Cuts at: 0 628 2832
Size: 628 2204
PasI CC'CwG_GG
Cuts at: 0 949 2164 2629 2832
Size: 949 1215 465 203
Fragments arranged by size:
1215 949 465 203
PflMI CCAn_nnn'nTGG
Cuts at: 0 2050 2832
Size: 2050 782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 2832
Size: 1510 32 1290
Fragments arranged by size:
1510 1290 32
PpuMI rG'GwC_Cy
Cuts at: 0 1108 1147 2335 2832
Size: 1108 39 1188 497
Fragments arranged by size:
1188 1108 497 39
PshAI GACnn'nnGTC
Cuts at: 0 2340 2832
Size: 2340 492
PsiI TTA'TAA
Cuts at: 0 650 2832
Size: 650 2182
PstI C_TGCA'G
Cuts at: 0 2429 2586 2832
Size: 2429 157 246
Fragments arranged by size:
2429 246 157
PvuII CAG'CTG
Cuts at: 0 898 2236 2449 2832
Size: 898 1338 213 383
Fragments arranged by size:
1338 898 383 213
SapI GCTCTTCn'nnn_
Cuts at: 0 1239 2093 2411 2832
Size: 1239 854 318 421
Fragments arranged by size:
1239 854 421 318
ScaI AGT'ACT
Cuts at: 0 2001 2832
Size: 2001 831
SfcI C'TryA_G
Cuts at: 0 719 2425 2438 2582 2832
Size: 719 1706 13 144 250
Fragments arranged by size:
1706 719 250 144 13
SfoI GGC'GCC
Cuts at: 0 2432 2832
Size: 2432 400
SmaI CCC'GGG
Cuts at: 0 2420 2621 2832
Size: 2420 201 211
Fragments arranged by size:
2420 211 201
SmlI C'TyrA_G
Cuts at: 0 1995 2330 2832
Size: 1995 335 502
Fragments arranged by size:
1995 502 335
SpeI A'CTAG_T
Cuts at: 0 249 2832
Size: 249 2583
StuI AGG'CCT
Cuts at: 0 1325 1619 2606 2832
Size: 1325 294 987 226
Fragments arranged by size:
1325 987 294 226
StyI C'CwwG_G
Cuts at: 0 1320 2832
Size: 1320 1512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1772 2813 2832
Size: 1772 1041 19
Fragments arranged by size:
1772 1041 19
TatI w'GTAC_w
Cuts at: 0 511 1054 1668 1999 2832
Size: 511 543 614 331 833
Fragments arranged by size:
833 614 543 511 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1056 1088 2073 2105 2292 2324 2832
Size: 1056 32 985 32 187 32 508
Fragments arranged by size:
1056 985 508 187 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1599 2832
Size: 1599 1233
XmaI C'CCGG_G
Cuts at: 0 2418 2619 2832
Size: 2418 201 213
Fragments arranged by size:
2418 213 201
XmnI GAAnn'nnTTC
Cuts at: 0 1017 2832
Size: 1017 1815
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApoI AseI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI
BstYI BtgI BtsI CspCI DraI DraIII EarI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
KasI KpnI MfeI MmeI MslI MspA1I NarI NspI
PasI PflMI PpiI PpuMI PshAI PsiI PstI PvuII
SapI ScaI SfcI SfoI SmaI SmlI SpeI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI
BcgI BfrBI BglI BmtI BplI BsaAI BsaBI BsaWI
BsiEI BsiHKAI BsiWI BspEI BspHI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DrdI EaeI EagI
EciI EcoICRI FalI FseI FspAI HindIII HpaI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SnaBI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI