Restriction map for
pF1KSDA2038
(Linear) (Six-base) MAPSORT of: KIAA2038.seq Check: 586 from: 1 to: 2622
KIAA2038.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 357 693 1910 2255 2622
Size: 291 66 336 1217 345 367
Fragments arranged by size:
1217 367 345 336 291 66
AfeI AGC'GCT
Cuts at: 0 502 1051 1867 2622
Size: 502 549 816 755
Fragments arranged by size:
816 755 549 502
AleI CACnn'nnGTG
Cuts at: 0 1078 2622
Size: 1078 1544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2423 2539 2622
Size: 2423 116 83
Fragments arranged by size:
2423 116 83
AlwNI CAG_nnn'CTG
Cuts at: 0 77 987 2140 2622
Size: 77 910 1153 482
Fragments arranged by size:
1153 910 482 77
ApaI G_GGCC'C
Cuts at: 0 224 928 1313 1758 1949 2622
Size: 224 704 385 445 191 673
Fragments arranged by size:
704 673 445 385 224 191
AvaI C'yCGr_G
Cuts at: 0 278 1162 1252 1312 1472 1751 1959 2293
Size: 278 884 90 60 160 279 208 334
Cuts at: 2293 2354 2495 2622
Size: 61 141 127
Fragments arranged by size:
884 334 279 278 208 160 141 127
90 61 60
BanI G'GyrC_C
Cuts at: 0 46 613 804 930 1089 2327 2622
Size: 46 567 191 126 159 1238 295
Fragments arranged by size:
1238 567 295 191 159 126 46
BanII G_rGCy'C
Cuts at: 0 224 452 928 1103 1169 1234 1259 1313
Size: 224 228 476 175 66 65 25 54
Cuts at: 1313 1343 1758 1887 1949 2099 2165 2468 2622
Size: 30 415 129 62 150 66 303 154
Fragments arranged by size:
476 415 303 228 224 175 154 150
129 66 66 65 62 54 30 25
BbeI G_GCGC'C
Cuts at: 0 50 617 934 1093 2331 2622
Size: 50 567 317 159 1238 291
Fragments arranged by size:
1238 567 317 291 159 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 1115 1931 2622
Size: 327 788 816 691
Fragments arranged by size:
816 788 691 327
BglI GCCn_nnn'nGGC
Cuts at: 0 22 813 923 1338 1753 2622
Size: 22 791 110 415 415 869
Fragments arranged by size:
869 791 415 415 110 22
Bme1580I G_kGCm'C
Cuts at: 0 224 809 928 1313 1758 1949 2622
Size: 224 585 119 385 445 191 673
Fragments arranged by size:
673 585 445 385 224 191 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 1248 2622
Size: 1248 1374
BmtI G_CTAG'C
Cuts at: 0 1192 2622
Size: 1192 1430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2457 2622
Size: 2457 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 217 748 1388 1445 1485 1545 1573
Size: 57 160 531 640 57 40 60 28
Cuts at: 1573 2113 2117 2452 2622
Size: 540 4 335 170
Fragments arranged by size:
640 540 531 335 170 160 60 57
57 40 28 4
Bpu10I CC'TnA_GC
Cuts at: 0 679 2141 2622
Size: 679 1462 481
Fragments arranged by size:
1462 679 481
BsaI GGTCTCn'nnnn_
Cuts at: 0 2445 2622
Size: 2445 177
BsaAI yAC'GTr
Cuts at: 0 1227 2622
Size: 1227 1395
BsaBI GATnn'nnATC
Cuts at: 0 232 2622
Size: 232 2390
BsaHI Gr'CG_yC
Cuts at: 0 47 128 614 931 1090 2328 2622
Size: 47 81 486 317 159 1238 294
Fragments arranged by size:
1238 486 317 294 159 81 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 1018 1048 1054 1084 1336 1366
Size: 463 30 525 30 6 30 252 30
Cuts at: 1366 1627 1657 2622
Size: 261 30 965
Fragments arranged by size:
965 525 463 261 252 30 30 30
30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 334 1303 1450 1569 1826 2031 2046
Size: 214 120 969 147 119 257 205 15
Cuts at: 2046 2049 2052 2318 2382 2449 2622
Size: 3 3 266 64 67 173
Fragments arranged by size:
969 266 257 214 205 173 147 120
119 67 64 15 3 3
BseYI C'CCAG_C
Cuts at: 0 531 573 808 900 1555 1704 1738 2001
Size: 531 42 235 92 655 149 34 263
Cuts at: 2001 2115 2622
Size: 114 507
Fragments arranged by size:
655 531 507 263 235 149 114 92
42 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 922 2233 2405 2545 2622
Size: 823 99 1311 172 140 77
Fragments arranged by size:
1311 823 172 140 99 77
BsiEI CG_ry'CG
Cuts at: 0 17 817 1079 1330 1494 1779 2622
Size: 17 800 262 251 164 285 843
Fragments arranged by size:
843 800 285 262 251 164 17
BsiHKAI G_wGCw'C
Cuts at: 0 452 755 1103 1887 2310 2622
Size: 452 303 348 784 423 312
Fragments arranged by size:
784 452 423 348 312 303
Bsp1286I G_dGCh'C
Cuts at: 0 224 452 755 809 928 1103 1169 1234
Size: 224 228 303 54 119 175 66 65
Cuts at: 1234 1259 1313 1343 1758 1887 1949 2099 2165
Size: 25 54 30 415 129 62 150 66
Cuts at: 2165 2310 2468 2622
Size: 145 158 154
Fragments arranged by size:
415 303 228 224 175 158 154 150
145 129 119 66 66 65 62 54
54 30 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 2565 2622
Size: 845 1720 57
Fragments arranged by size:
1720 845 57
BsrBI CCG'CTC
Cuts at: 0 57 1775 2622
Size: 57 1718 847
Fragments arranged by size:
1718 847 57
BsrDI GCAATG_nn'
Cuts at: 0 858 1488 2622
Size: 858 630 1134
Fragments arranged by size:
1134 858 630
BsrFI r'CCGG_y
Cuts at: 0 25 164 621 789 812 1194 1204 1493
Size: 25 139 457 168 23 382 10 289
Cuts at: 1493 1542 2622
Size: 49 1080
Fragments arranged by size:
1080 457 382 289 168 139 49 25
23 10
BsrGI T'GTAC_A
Cuts at: 0 419 2622
Size: 419 2203
BssHII G'CGCG_C
Cuts at: 0 762 1130 1132 1155 2622
Size: 762 368 2 23 1467
Fragments arranged by size:
1467 762 368 23 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 759 846 945 987 2622
Size: 759 87 99 42 1635
Fragments arranged by size:
1635 759 99 87 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1667 2172 2622
Size: 894 773 505 450
Fragments arranged by size:
894 773 505 450
BstYI r'GATC_y
Cuts at: 0 473 1277 2622
Size: 473 804 1345
Fragments arranged by size:
1345 804 473
Bsu36I CC'TnA_GG
Cuts at: 0 1917 2622
Size: 1917 705
BtgI C'CryG_G
Cuts at: 0 590 1073 1364 2622
Size: 590 483 291 1258
Fragments arranged by size:
1258 590 483 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1613 2622
Size: 1613 1009
BtsI GCAGTG_nn'
Cuts at: 0 175 1617 1623 2177 2622
Size: 175 1442 6 554 445
Fragments arranged by size:
1442 554 445 175 6
ClaI AT'CG_AT
Cuts at: 0 2507 2622
Size: 2507 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2355 2390 2622
Size: 2355 35 232
Fragments arranged by size:
2355 232 35
DraIII CAC_nnn'GTG
Cuts at: 0 759 846 2622
Size: 759 87 1776
Fragments arranged by size:
1776 759 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 67 1006 1376 2622
Size: 67 939 370 1246
Fragments arranged by size:
1246 939 370 67
EaeI y'GGCC_r
Cuts at: 0 14 23 326 551 814 992 1076 1327
Size: 14 9 303 225 263 178 84 251
Cuts at: 1327 1491 2622
Size: 164 1131
Fragments arranged by size:
1131 303 263 251 225 178 164 84
14 9
EagI C'GGCC_G
Cuts at: 0 14 814 1076 1327 1491 2622
Size: 14 800 262 251 164 1131
Fragments arranged by size:
1131 800 262 251 164 14
EarI CTCTTCn'nnn_
Cuts at: 0 2070 2622
Size: 2070 552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 2622
Size: 484 2138
EcoICRI GAG'CTC
Cuts at: 0 450 1101 1885 2622
Size: 450 651 784 737
Fragments arranged by size:
784 737 651 450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 217 291 357 693 748 1388 1445
Size: 57 160 74 66 336 55 640 57
Cuts at: 1445 1485 1545 1573 1910 2113 2117 2255 2452
Size: 40 60 28 337 203 4 138 197
Cuts at: 2452 2622
Size: 170
Fragments arranged by size:
640 337 336 203 197 170 160 138
74 66 60 57 57 55 40 28
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 271 2434 2622
Size: 199 72 2163 188
Fragments arranged by size:
2163 199 188 72
EcoO109I rG'GnC_Cy
Cuts at: 0 924 984 999 1309 1317 1755 1921 1963
Size: 924 60 15 310 8 438 166 42
Cuts at: 1963 2256 2290 2338 2622
Size: 293 34 48 284
Fragments arranged by size:
924 438 310 293 284 166 60 48
42 34 15 8
FseI GG_CCGG'CC
Cuts at: 0 29 2622
Size: 29 2593
HaeII r_GCGC'y
Cuts at: 0 50 504 550 617 913 934 1017 1034
Size: 50 454 46 67 296 21 83 17
Cuts at: 1034 1053 1093 1195 1396 1869 2331 2622
Size: 19 40 102 201 473 462 291
Fragments arranged by size:
473 462 454 296 291 201 102 83
67 50 46 40 21 19 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1018 1050 1054 1086 1336 1368 1627 1659
Size: 1018 32 4 32 250 32 259 32
Cuts at: 1659 2377 2409 2489 2521 2622
Size: 718 32 80 32 101
Fragments arranged by size:
1018 718 259 250 101 80 32 32
32 32 32 32 4
KasI G'GCGC_C
Cuts at: 0 46 613 930 1089 2327 2622
Size: 46 567 317 159 1238 295
Fragments arranged by size:
1238 567 317 295 159 46
MscI TGG'CCA
Cuts at: 0 328 2622
Size: 328 2294
MslI CAynn'nnrTG
Cuts at: 0 352 457 652 1078 2521 2622
Size: 352 105 195 426 1443 101
Fragments arranged by size:
1443 426 352 195 105 101
MspA1I CmG'CkG
Cuts at: 0 77 592 726 936 1096 1366 1490 1829
Size: 77 515 134 210 160 270 124 339
Cuts at: 1829 1930 2029 2278 2622
Size: 101 99 249 344
Fragments arranged by size:
515 344 339 270 249 210 160 134
124 101 99 77
NaeI GCC'GGC
Cuts at: 0 27 166 814 1196 1206 1495 2622
Size: 27 139 648 382 10 289 1127
Fragments arranged by size:
1127 648 382 289 139 27 10
NarI GG'CG_CC
Cuts at: 0 47 614 931 1090 2328 2622
Size: 47 567 317 159 1238 294
Fragments arranged by size:
1238 567 317 294 159 47
NgoMIV G'CCGG_C
Cuts at: 0 25 164 812 1194 1204 1493 2622
Size: 25 139 648 382 10 289 1129
Fragments arranged by size:
1129 648 382 289 139 25 10
NheI G'CTAG_C
Cuts at: 0 1188 2622
Size: 1188 1434
NspI r_CATG'y
Cuts at: 0 439 2622
Size: 439 2183
PasI CC'CwG_GG
Cuts at: 0 250 1336 2622
Size: 250 1086 1286
Fragments arranged by size:
1286 1086 250
PfoI T'CCnGG_A
Cuts at: 0 266 1589 2090 2622
Size: 266 1323 501 532
Fragments arranged by size:
1323 532 501 266
PpuMI rG'GwC_Cy
Cuts at: 0 999 1963 2256 2290 2622
Size: 999 964 293 34 332
Fragments arranged by size:
999 964 332 293 34
PspOMI G'GGCC_C
Cuts at: 0 220 924 1309 1754 1945 2622
Size: 220 704 385 445 191 677
Fragments arranged by size:
704 677 445 385 220 191
PstI C_TGCA'G
Cuts at: 0 245 737 1541 2288 2576 2622
Size: 245 492 804 747 288 46
Fragments arranged by size:
804 747 492 288 245 46
PvuII CAG'CTG
Cuts at: 0 1829 2029 2278 2622
Size: 1829 200 249 344
Fragments arranged by size:
1829 344 249 200
SacI G_AGCT'C
Cuts at: 0 452 1103 1887 2622
Size: 452 651 784 735
Fragments arranged by size:
784 735 651 452
SacII CC_GC'GG
Cuts at: 0 593 1367 2622
Size: 593 774 1255
Fragments arranged by size:
1255 774 593
SanDI GG'GwC_CC
Cuts at: 0 999 2290 2622
Size: 999 1291 332
Fragments arranged by size:
1291 999 332
ScaI AGT'ACT
Cuts at: 0 1069 2622
Size: 1069 1553
SexAI A'CCwGG_T
Cuts at: 0 2252 2622
Size: 2252 370
SfcI C'TryA_G
Cuts at: 0 241 733 1537 2284 2572 2622
Size: 241 492 804 747 288 50
Fragments arranged by size:
804 747 492 288 241 50
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 22 1753 2622
Size: 22 1731 869
Fragments arranged by size:
1731 869 22
SfoI GGC'GCC
Cuts at: 0 48 615 932 1091 2329 2622
Size: 48 567 317 159 1238 293
Fragments arranged by size:
1238 567 317 293 159 48
SgrAI Cr'CCGG_yG
Cuts at: 0 1204 2622
Size: 1204 1418
SmaI CCC'GGG
Cuts at: 0 280 1164 1474 1753 2356 2622
Size: 280 884 310 279 603 266
Fragments arranged by size:
884 603 310 280 279 266
SphI G_CATG'C
Cuts at: 0 439 2622
Size: 439 2183
StuI AGG'CCT
Cuts at: 0 1747 2020 2224 2622
Size: 1747 273 204 398
Fragments arranged by size:
1747 398 273 204
StyI C'CwwG_G
Cuts at: 0 102 494 2219 2322 2622
Size: 102 392 1725 103 300
Fragments arranged by size:
1725 392 300 103 102
TatI w'GTAC_w
Cuts at: 0 419 1067 2622
Size: 419 648 1555
Fragments arranged by size:
1555 648 419
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 1947 1979 2622
Size: 463 32 1452 32 643
Fragments arranged by size:
1452 643 463 32 32
XmaI C'CCGG_G
Cuts at: 0 278 1162 1472 1751 2354 2622
Size: 278 884 310 279 603 268
Fragments arranged by size:
884 603 310 279 278 268
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SanDI ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SphI StuI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BpuEI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BssSI BstBI BstEII BstZ17I DraI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SapI SbfI SgfI SmlI
SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI
XcmI XhoI XmnI ZraI