Restriction map for
pF1KSDB0003
(Linear) (Six-base) MAPSORT of: KIBB0003.seq Check: 6541 from: 1 to: 1506
KIBB0003.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 449 1506
Size: 449 1057
AatII G_ACGT'C
Cuts at: 0 698 1506
Size: 698 808
AccI GT'mk_AC
Cuts at: 0 1272 1506
Size: 1272 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1506
Size: 1084 422
AflII C'TTAA_G
Cuts at: 0 1062 1506
Size: 1062 444
AleI CACnn'nnGTG
Cuts at: 0 558 1506
Size: 558 948
AlwNI CAG_nnn'CTG
Cuts at: 0 978 1506
Size: 978 528
ApaI G_GGCC'C
Cuts at: 0 1326 1506
Size: 1326 180
ApoI r'AATT_y
Cuts at: 0 1021 1186 1216 1506
Size: 1021 165 30 290
Fragments arranged by size:
1021 290 165 30
AvaI C'yCGr_G
Cuts at: 0 81 1506
Size: 81 1425
BanI G'GyrC_C
Cuts at: 0 98 346 1506
Size: 98 248 1160
Fragments arranged by size:
1160 248 98
BanII G_rGCy'C
Cuts at: 0 985 1326 1506
Size: 985 341 180
Fragments arranged by size:
985 341 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 1506
Size: 458 1048
BclI T'GATC_A
Cuts at: 0 137 1506
Size: 137 1369
BfrBI ATG'CAT
Cuts at: 0 397 1506
Size: 397 1109
Bme1580I G_kGCm'C
Cuts at: 0 1326 1506
Size: 1326 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 1383 1506
Size: 1383 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1090 1506
Size: 901 189 416
Fragments arranged by size:
901 416 189
BsaHI Gr'CG_yC
Cuts at: 0 695 1506
Size: 695 811
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 1498 1506
Size: 66 1432 8
Fragments arranged by size:
1432 66 8
BseYI C'CCAG_C
Cuts at: 0 27 978 1362 1506
Size: 27 951 384 144
Fragments arranged by size:
951 384 144 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1506
Size: 421 1085
BsiEI CG_ry'CG
Cuts at: 0 94 1506
Size: 94 1412
BsmI GAATG_Cn'
Cuts at: 0 200 1506
Size: 200 1306
Bsp1286I G_dGCh'C
Cuts at: 0 985 1326 1506
Size: 985 341 180
Fragments arranged by size:
985 341 180
BspHI T'CATG_A
Cuts at: 0 1286 1506
Size: 1286 220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 1506
Size: 449 1057
BsrBI CCG'CTC
Cuts at: 0 1308 1506
Size: 1308 198
BsrFI r'CCGG_y
Cuts at: 0 89 1506
Size: 89 1417
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1095 1506
Size: 1095 411
BstEII G'GTnAC_C
Cuts at: 0 612 1506
Size: 612 894
BstZ17I GTA'TAC
Cuts at: 0 1273 1506
Size: 1273 233
BtgI C'CryG_G
Cuts at: 0 282 579 1506
Size: 282 297 927
Fragments arranged by size:
927 297 282
BtsI GCAGTG_nn'
Cuts at: 0 711 1168 1440 1506
Size: 711 457 272 66
Fragments arranged by size:
711 457 272 66
DraIII CAC_nnn'GTG
Cuts at: 0 798 1506
Size: 798 708
DrdI GACnn_nn'nnGTC
Cuts at: 0 254 1506
Size: 254 1252
EaeI y'GGCC_r
Cuts at: 0 91 1506
Size: 91 1415
EagI C'GGCC_G
Cuts at: 0 91 1506
Size: 91 1415
EarI CTCTTCn'nnn_
Cuts at: 0 574 1315 1390 1506
Size: 574 741 75 116
Fragments arranged by size:
741 574 116 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 298 1506
Size: 298 1208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1084 1090 1506
Size: 901 183 6 416
Fragments arranged by size:
901 416 183 6
EcoRI G'AATT_C
Cuts at: 0 1186 1506
Size: 1186 320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 475 1222 1506
Size: 475 747 284
Fragments arranged by size:
747 475 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 617 649 1506
Size: 617 32 857
Fragments arranged by size:
857 617 32
HindIII A'AGCT_T
Cuts at: 0 375 733 1506
Size: 375 358 773
Fragments arranged by size:
773 375 358
MfeI C'AATT_G
Cuts at: 0 905 1506
Size: 905 601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1506
Size: 1331 175
MslI CAynn'nnrTG
Cuts at: 0 558 1506
Size: 558 948
MspA1I CmG'CkG
Cuts at: 0 114 978 1442 1506
Size: 114 864 464 64
Fragments arranged by size:
864 464 114 64
NaeI GCC'GGC
Cuts at: 0 91 1506
Size: 91 1415
NcoI C'CATG_G
Cuts at: 0 579 1506
Size: 579 927
NdeI CA'TA_TG
Cuts at: 0 476 1506
Size: 476 1030
NgoMIV G'CCGG_C
Cuts at: 0 89 1506
Size: 89 1417
NsiI A_TGCA'T
Cuts at: 0 399 1506
Size: 399 1107
NspI r_CATG'y
Cuts at: 0 915 1362 1506
Size: 915 447 144
Fragments arranged by size:
915 447 144
PasI CC'CwG_GG
Cuts at: 0 119 808 1506
Size: 119 689 698
Fragments arranged by size:
698 689 119
PfoI T'CCnGG_A
Cuts at: 0 234 1313 1506
Size: 234 1079 193
Fragments arranged by size:
1079 234 193
PspOMI G'GGCC_C
Cuts at: 0 1322 1506
Size: 1322 184
PstI C_TGCA'G
Cuts at: 0 182 786 1506
Size: 182 604 720
Fragments arranged by size:
720 604 182
PvuII CAG'CTG
Cuts at: 0 978 1506
Size: 978 528
SapI GCTCTTCn'nnn_
Cuts at: 0 1315 1390 1506
Size: 1315 75 116
Fragments arranged by size:
1315 116 75
SfcI C'TryA_G
Cuts at: 0 178 782 1191 1506
Size: 178 604 409 315
Fragments arranged by size:
604 409 315 178
SmaI CCC'GGG
Cuts at: 0 83 1506
Size: 83 1423
SmlI C'TyrA_G
Cuts at: 0 1062 1506
Size: 1062 444
SphI G_CATG'C
Cuts at: 0 915 1506
Size: 915 591
StyI C'CwwG_G
Cuts at: 0 459 579 1467 1506
Size: 459 120 888 39
Fragments arranged by size:
888 459 120 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 41 715 821 1506
Size: 41 674 106 685
Fragments arranged by size:
685 674 106 41
TatI w'GTAC_w
Cuts at: 0 322 1506
Size: 322 1184
XmaI C'CCGG_G
Cuts at: 0 81 1506
Size: 81 1425
ZraI GAC'GTC
Cuts at: 0 696 1506
Size: 696 810
Enzymes that do cut:
AarI AatII AccI AcuI AflII AleI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BclI BfrBI Bme1580I
BmrI BpmI BsaHI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstZ17I
BtgI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI Hin4I HindIII MfeI MmeI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI
PfoI PspOMI PstI PvuII SapI SfcI SmaI SmlI
SphI StyI TaqII TatI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsiHKAI BsiWI BsmBI BspEI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstYI Bsu36I BtgZI ClaI CspCI DraI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MscI NarI NheI
NotI NruI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmnI