Restriction map for
pF1KSDB0005
(Linear) (Six-base) MAPSORT of: KIBB0005.seq Check: 8333 from: 1 to: 3813
KIBB0005.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 3320 3813
Size: 3320 493
AccI GT'mk_AC
Cuts at: 0 725 2846 3813
Size: 725 2121 967
Fragments arranged by size:
2121 967 725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 2002 2149 2461 2484 3322 3403 3813
Size: 787 1215 147 312 23 838 81 410
Fragments arranged by size:
1215 838 787 410 312 147 81 23
AfeI AGC'GCT
Cuts at: 0 109 157 3813
Size: 109 48 3656
Fragments arranged by size:
3656 109 48
AflIII A'CryG_T
Cuts at: 0 2531 3813
Size: 2531 1282
AhdI GACnn_n'nnGTC
Cuts at: 0 3702 3813
Size: 3702 111
AleI CACnn'nnGTG
Cuts at: 0 1210 3813
Size: 1210 2603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 2009 3813
Size: 88 1921 1804
Fragments arranged by size:
1921 1804 88
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 2038 2070 3813
Size: 1000 32 1006 32 1743
Fragments arranged by size:
1743 1006 1000 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 102 1749 2127 2643 2761 2913 3027 3041
Size: 102 1647 378 516 118 152 114 14
Cuts at: 3041 3300 3735 3813
Size: 259 435 78
Fragments arranged by size:
1647 516 435 378 259 152 118 114
102 78 14
ApaI G_GGCC'C
Cuts at: 0 883 2166 3813
Size: 883 1283 1647
Fragments arranged by size:
1647 1283 883
ApaLI G'TGCA_C
Cuts at: 0 2506 3813
Size: 2506 1307
ApoI r'AATT_y
Cuts at: 0 2881 3813
Size: 2881 932
AvaI C'yCGr_G
Cuts at: 0 269 337 382 400 502 660 1434 3406
Size: 269 68 45 18 102 158 774 1972
Cuts at: 3406 3813
Size: 407
Fragments arranged by size:
1972 774 407 269 158 102 68 45
18
BamHI G'GATC_C
Cuts at: 0 479 1893 3813
Size: 479 1414 1920
Fragments arranged by size:
1920 1414 479
BanI G'GyrC_C
Cuts at: 0 128 256 1172 1258 1343 2334 3198 3813
Size: 128 128 916 86 85 991 864 615
Fragments arranged by size:
991 916 864 615 128 128 86 85
BanII G_rGCy'C
Cuts at: 0 59 269 338 661 883 1443 1658 1691
Size: 59 210 69 323 222 560 215 33
Cuts at: 1691 2166 2726 2905 3413 3813
Size: 475 560 179 508 400
Fragments arranged by size:
560 560 508 475 400 323 222 215
210 179 69 59 33
BbeI G_GCGC'C
Cuts at: 0 132 260 1262 3813
Size: 132 128 1002 2551
Fragments arranged by size:
2551 1002 132 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 2175 3144 3813
Size: 2175 969 669
Fragments arranged by size:
2175 969 669
BbvCI CC'TCA_GC
Cuts at: 0 1396 3813
Size: 1396 2417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 429 831 865 1265 1299 3039 3073
Size: 395 34 402 34 400 34 1740 34
Cuts at: 3073 3813
Size: 740
Fragments arranged by size:
1740 740 402 400 395 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1297 2912 3813
Size: 1297 1615 901
Fragments arranged by size:
1615 1297 901
BfrBI ATG'CAT
Cuts at: 0 676 3813
Size: 676 3137
BglI GCCn_nnn'nGGC
Cuts at: 0 451 810 2519 3813
Size: 451 359 1709 1294
Fragments arranged by size:
1709 1294 451 359
BglII A'GATC_T
Cuts at: 0 1235 2369 3813
Size: 1235 1134 1444
Fragments arranged by size:
1444 1235 1134
BlpI GC'TnA_GC
Cuts at: 0 2861 3813
Size: 2861 952
Bme1580I G_kGCm'C
Cuts at: 0 883 2166 2321 2339 2510 3203 3813
Size: 883 1283 155 18 171 693 610
Fragments arranged by size:
1283 883 693 610 171 155 18
BmgBI CAC'GTC
Cuts at: 0 2181 3360 3813
Size: 2181 1179 453
Fragments arranged by size:
2181 1179 453
BmtI G_CTAG'C
Cuts at: 0 1526 3813
Size: 1526 2287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 1050 3813
Size: 93 957 2763
Fragments arranged by size:
2763 957 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 154 841 1002 1465 1570 2329 2650
Size: 124 30 687 161 463 105 759 321
Cuts at: 2650 2818 2917 3775 3813
Size: 168 99 858 38
Fragments arranged by size:
858 759 687 463 321 168 161 124
105 99 38 30
Bpu10I CC'TnA_GC
Cuts at: 0 460 1396 1540 1652 3813
Size: 460 936 144 112 2161
Fragments arranged by size:
2161 936 460 144 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2203 3813
Size: 2203 1610
BsaI GGTCTCn'nnnn_
Cuts at: 0 1437 1624 3813
Size: 1437 187 2189
Fragments arranged by size:
2189 1437 187
BsaAI yAC'GTr
Cuts at: 0 531 3813
Size: 531 3282
BsaBI GATnn'nnATC
Cuts at: 0 2368 3813
Size: 2368 1445
BsaHI Gr'CG_yC
Cuts at: 0 129 257 512 722 743 785 1217 1259
Size: 129 128 255 210 21 42 432 42
Cuts at: 1259 2027 3317 3813
Size: 768 1290 496
Fragments arranged by size:
1290 768 496 432 255 210 129 128
42 42 21
BsaWI w'CCGG_w
Cuts at: 0 3274 3813
Size: 3274 539
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 982 1000 1012 1030 1363 1393
Size: 244 30 708 18 12 18 333 30
Cuts at: 1393 2449 2479 3703 3733 3813
Size: 1056 30 1224 30 80
Fragments arranged by size:
1224 1056 708 333 244 80 30 30
30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 693 1026 1039 1345 1371 1543 1968
Size: 346 347 333 13 306 26 172 425
Cuts at: 1968 2083 2359 2535 3241 3265 3813
Size: 115 276 176 706 24 548
Fragments arranged by size:
706 548 425 347 346 333 306 276
176 172 115 26 24 13
BseYI C'CCAG_C
Cuts at: 0 644 1761 2488 3673 3813
Size: 644 1117 727 1185 140
Fragments arranged by size:
1185 1117 727 644 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1723 2193 2582 2775 3583 3813
Size: 1723 470 389 193 808 230
Fragments arranged by size:
1723 808 470 389 230 193
BsiEI CG_ry'CG
Cuts at: 0 432 3813
Size: 432 3381
BsiHKAI G_wGCw'C
Cuts at: 0 904 1658 1960 2117 2510 2980 3813
Size: 904 754 302 157 393 470 833
Fragments arranged by size:
904 833 754 470 393 302 157
BsmI GAATG_Cn'
Cuts at: 0 2681 3813
Size: 2681 1132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1265 3813
Size: 1265 2548
Bsp1286I G_dGCh'C
Cuts at: 0 59 269 338 661 883 904 1443 1658
Size: 59 210 69 323 222 21 539 215
Cuts at: 1658 1691 1960 2117 2166 2321 2339 2510 2726
Size: 33 269 157 49 155 18 171 216
Cuts at: 2726 2905 2980 3203 3413 3813
Size: 179 75 223 210 400
Fragments arranged by size:
539 400 323 269 223 222 216 215
210 210 179 171 157 155 75 69
59 49 33 21 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 929 2207 3506 3533 3813
Size: 182 747 1278 1299 27 280
Fragments arranged by size:
1299 1278 747 280 182 27
BsrBI CCG'CTC
Cuts at: 0 477 885 1103 2231 2274 3813
Size: 477 408 218 1128 43 1539
Fragments arranged by size:
1539 1128 477 408 218 43
BsrFI r'CCGG_y
Cuts at: 0 212 1175 1340 1605 2117 3813
Size: 212 963 165 265 512 1696
Fragments arranged by size:
1696 963 512 265 212 165
BssHII G'CGCG_C
Cuts at: 0 234 3813
Size: 234 3579
BssSI C'ACGA_G
Cuts at: 0 550 2652 3813
Size: 550 2102 1161
Fragments arranged by size:
2102 1161 550
BstAPI GCAn_nnn'nTGC
Cuts at: 0 183 3027 3813
Size: 183 2844 786
Fragments arranged by size:
2844 786 183
BstEII G'GTnAC_C
Cuts at: 0 3174 3813
Size: 3174 639
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1212 1761 1809 3813
Size: 627 585 549 48 2004
Fragments arranged by size:
2004 627 585 549 48
BstYI r'GATC_y
Cuts at: 0 479 1235 1664 1893 2280 2369 2954 3813
Size: 479 756 429 229 387 89 585 859
Fragments arranged by size:
859 756 585 479 429 387 229 89
Bsu36I CC'TnA_GG
Cuts at: 0 2914 3813
Size: 2914 899
BtgI C'CryG_G
Cuts at: 0 492 1266 1802 2627 3284 3611 3813
Size: 492 774 536 825 657 327 202
Fragments arranged by size:
825 774 657 536 492 327 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 234 1370 3042 3354 3438 3813
Size: 234 1136 1672 312 84 375
Fragments arranged by size:
1672 1136 375 312 234 84
BtsI GCAGTG_nn'
Cuts at: 0 1165 1179 1555 1714 2321 2467 3813
Size: 1165 14 376 159 607 146 1346
Fragments arranged by size:
1346 1165 607 376 159 146 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3600 3635 3813
Size: 3600 35 178
Fragments arranged by size:
3600 178 35
EaeI y'GGCC_r
Cuts at: 0 188 495 811 1041 1751 3281 3813
Size: 188 307 316 230 710 1530 532
Fragments arranged by size:
1530 710 532 316 307 230 188
EarI CTCTTCn'nnn_
Cuts at: 0 196 1696 1744 2398 3115 3421 3813
Size: 196 1500 48 654 717 306 392
Fragments arranged by size:
1500 717 654 392 306 196 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 523 3813
Size: 523 3290
EcoICRI GAG'CTC
Cuts at: 0 1656 3813
Size: 1656 2157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 154 787 841 1002 1465 1570 2002
Size: 124 30 633 54 161 463 105 432
Cuts at: 2002 2149 2329 2461 2484 2650 2818 2917 3322
Size: 147 180 132 23 166 168 99 405
Cuts at: 3322 3403 3775 3813
Size: 81 372 38
Fragments arranged by size:
633 463 432 405 372 180 168 166
161 147 132 124 105 99 81 54
38 30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 565 3514 3813
Size: 136 429 2949 299
Fragments arranged by size:
2949 429 299 136
EcoO109I rG'GnC_Cy
Cuts at: 0 246 436 683 803 879 941 1503 2162
Size: 246 190 247 120 76 62 562 659
Cuts at: 2162 3024 3813
Size: 862 789
Fragments arranged by size:
862 789 659 562 247 246 190 120
76 62
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2154 3327 3408 3813
Size: 2154 1173 81 405
Fragments arranged by size:
2154 1173 405 81
FspI TGC'GCA
Cuts at: 0 892 3813
Size: 892 2921
HaeII r_GCGC'y
Cuts at: 0 111 132 159 260 301 460 924 1262
Size: 111 21 27 101 41 159 464 338
Cuts at: 1262 1341 3240 3813
Size: 79 1899 573
Fragments arranged by size:
1899 573 464 338 159 111 101 79
41 27 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 982 1014 1021 1053 1363 1395
Size: 244 32 706 32 7 32 310 32
Cuts at: 1395 2449 2481 2698 2730 2766 2798 3688 3703
Size: 1054 32 217 32 36 32 890 15
Cuts at: 3703 3720 3735 3776 3808 3813
Size: 17 15 41 32 5
Fragments arranged by size:
1054 890 706 310 244 217 41 36
32 32 32 32 32 32 32 32
17 15 15 7 5
HincII GTy'rAC
Cuts at: 0 573 726 3813
Size: 573 153 3087
Fragments arranged by size:
3087 573 153
HindIII A'AGCT_T
Cuts at: 0 2610 3813
Size: 2610 1203
KasI G'GCGC_C
Cuts at: 0 128 256 1258 3813
Size: 128 128 1002 2555
Fragments arranged by size:
2555 1002 128 128
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 569 1921 2435 3813
Size: 491 78 1352 514 1378
Fragments arranged by size:
1378 1352 514 491 78
MscI TGG'CCA
Cuts at: 0 190 497 1043 1753 3283 3813
Size: 190 307 546 710 1530 530
Fragments arranged by size:
1530 710 546 530 307 190
MslI CAynn'nnrTG
Cuts at: 0 625 1210 1349 1807 2098 3061 3813
Size: 625 585 139 458 291 963 752
Fragments arranged by size:
963 752 625 585 458 291 139
MspA1I CmG'CkG
Cuts at: 0 716 1588 1725 1761 2124 2688 2860 3012
Size: 716 872 137 36 363 564 172 152
Cuts at: 3012 3701 3753 3813
Size: 689 52 60
Fragments arranged by size:
872 716 689 564 363 172 152 137
60 52 36
NaeI GCC'GGC
Cuts at: 0 1342 3813
Size: 1342 2471
NarI GG'CG_CC
Cuts at: 0 129 257 1259 3813
Size: 129 128 1002 2554
Fragments arranged by size:
2554 1002 129 128
NcoI C'CATG_G
Cuts at: 0 492 1266 1802 3813
Size: 492 774 536 2011
Fragments arranged by size:
2011 774 536 492
NgoMIV G'CCGG_C
Cuts at: 0 1340 3813
Size: 1340 2473
NheI G'CTAG_C
Cuts at: 0 1522 3813
Size: 1522 2291
NsiI A_TGCA'T
Cuts at: 0 678 3813
Size: 678 3135
NspI r_CATG'y
Cuts at: 0 1086 3492 3813
Size: 1086 2406 321
Fragments arranged by size:
2406 1086 321
PasI CC'CwG_GG
Cuts at: 0 606 3813
Size: 606 3207
PflMI CCAn_nnn'nTGG
Cuts at: 0 1749 2298 3222 3813
Size: 1749 549 924 591
Fragments arranged by size:
1749 924 591 549
PfoI T'CCnGG_A
Cuts at: 0 44 1088 2582 3813
Size: 44 1044 1494 1231
Fragments arranged by size:
1494 1231 1044 44
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 3813
Size: 1000 32 2781
Fragments arranged by size:
2781 1000 32
PpuMI rG'GwC_Cy
Cuts at: 0 436 941 1503 3813
Size: 436 505 562 2310
Fragments arranged by size:
2310 562 505 436
PshAI GACnn'nnGTC
Cuts at: 0 2088 2842 3148 3813
Size: 2088 754 306 665
Fragments arranged by size:
2088 754 665 306
PspOMI G'GGCC_C
Cuts at: 0 879 2162 3813
Size: 879 1283 1651
Fragments arranged by size:
1651 1283 879
PspXI vC'TCGA_Gb
Cuts at: 0 660 3813
Size: 660 3153
PstI C_TGCA'G
Cuts at: 0 179 997 1320 2024 2639 3032 3752 3813
Size: 179 818 323 704 615 393 720 61
Fragments arranged by size:
818 720 704 615 393 323 179 61
PvuII CAG'CTG
Cuts at: 0 716 1761 2124 2860 3012 3701 3753 3813
Size: 716 1045 363 736 152 689 52 60
Fragments arranged by size:
1045 736 716 689 363 152 60 52
RsrII CG'GwC_CG
Cuts at: 0 591 3813
Size: 591 3222
SacI G_AGCT'C
Cuts at: 0 1658 3813
Size: 1658 2155
SalI G'TCGA_C
Cuts at: 0 724 3813
Size: 724 3089
SanDI GG'GwC_CC
Cuts at: 0 436 3813
Size: 436 3377
SapI GCTCTTCn'nnn_
Cuts at: 0 196 1696 3813
Size: 196 1500 2117
Fragments arranged by size:
2117 1500 196
SbfI CC_TGCA'GG
Cuts at: 0 3032 3813
Size: 3032 781
SfcI C'TryA_G
Cuts at: 0 175 993 1316 2020 2635 2847 3028 3748
Size: 175 818 323 704 615 212 181 720
Cuts at: 3748 3813
Size: 65
Fragments arranged by size:
818 720 704 615 323 212 181 175
65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 810 3813
Size: 810 3003
SfoI GGC'GCC
Cuts at: 0 130 258 1260 3813
Size: 130 128 1002 2553
Fragments arranged by size:
2553 1002 130 128
SmaI CCC'GGG
Cuts at: 0 384 402 504 3813
Size: 384 18 102 3309
Fragments arranged by size:
3309 384 102 18
SmlI C'TyrA_G
Cuts at: 0 660 2218 3813
Size: 660 1558 1595
Fragments arranged by size:
1595 1558 660
SphI G_CATG'C
Cuts at: 0 3492 3813
Size: 3492 321
StuI AGG'CCT
Cuts at: 0 118 3813
Size: 118 3695
StyI C'CwwG_G
Cuts at: 0 191 492 808 1266 1802 1889 2960 3194
Size: 191 301 316 458 536 87 1071 234
Cuts at: 3194 3707 3813
Size: 513 106
Fragments arranged by size:
1071 536 513 458 316 301 234 191
106 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1753 3078 3813
Size: 1753 1325 735
Fragments arranged by size:
1753 1325 735
TatI w'GTAC_w
Cuts at: 0 2786 3389 3740 3813
Size: 2786 603 351 73
Fragments arranged by size:
2786 603 351 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2033 2065 3813
Size: 2033 32 1748
Fragments arranged by size:
2033 1748 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 1911 2328 2758 3813
Size: 129 1782 417 430 1055
Fragments arranged by size:
1782 1055 430 417 129
XhoI C'TCGA_G
Cuts at: 0 660 3813
Size: 660 3153
XmaI C'CCGG_G
Cuts at: 0 382 400 502 3813
Size: 382 18 102 3311
Fragments arranged by size:
3311 382 102 18
XmnI GAAnn'nnTTC
Cuts at: 0 523 2602 3813
Size: 523 2079 1211
Fragments arranged by size:
2079 1211 523
ZraI GAC'GTC
Cuts at: 0 3318 3813
Size: 3318 495
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
RsrII SacI SalI SanDI SapI SbfI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AscI AseI AsiSI
AvrII BaeI BclI BmrI BsiWI BspEI BspHI BsrDI
BsrGI BstBI BstZ17I ClaI DraI DraIII DrdI EagI
EcoRI EcoRV FseI FspAI HpaI KpnI MfeI MluI
NdeI NotI NruI PacI PciI PmeI PmlI PsiI
PsrI PvuI SacII ScaI SexAI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI