Restriction map for
pF1KB0006
(Linear) (Six-base) MAPSORT of: KIBB0006.seq Check: 5159 from: 1 to: 6795
KIBB0006.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1643 6795
Size: 1643 5152
AccI GT'mk_AC
Cuts at: 0 944 1673 2027 2410 3449 4022 4501 6795
Size: 944 729 354 383 1039 573 479 2294
Fragments arranged by size:
2294 1039 944 729 573 479 383 354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 299 988 2032 3380 3938 4173 4933
Size: 293 6 689 1044 1348 558 235 760
Cuts at: 4933 5677 6795
Size: 744 1118
Fragments arranged by size:
1348 1118 1044 760 744 689 558 293
235 6
AflII C'TTAA_G
Cuts at: 0 5113 6795
Size: 5113 1682
AflIII A'CryG_T
Cuts at: 0 488 1489 6466 6517 6795
Size: 488 1001 4977 51 278
Fragments arranged by size:
4977 1001 488 278 51
AhdI GACnn_n'nnGTC
Cuts at: 0 1859 2238 6795
Size: 1859 379 4557
Fragments arranged by size:
4557 1859 379
AleI CACnn'nnGTG
Cuts at: 0 823 2113 2320 3289 3307 3565 4190 6795
Size: 823 1290 207 969 18 258 625 2605
Fragments arranged by size:
2605 1290 969 823 625 258 207 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 435 4151 6795
Size: 435 3716 2644
Fragments arranged by size:
3716 2644 435
AlwNI CAG_nnn'CTG
Cuts at: 0 48 966 1696 2295 2431 2859 4342 4869
Size: 48 918 730 599 136 428 1483 527
Cuts at: 4869 5359 6795
Size: 490 1436
Fragments arranged by size:
1483 1436 918 730 599 527 490 428
136 48
ApaI G_GGCC'C
Cuts at: 0 20 3066 6009 6795
Size: 20 3046 2943 786
Fragments arranged by size:
3046 2943 786 20
ApaLI G'TGCA_C
Cuts at: 0 1485 4126 6795
Size: 1485 2641 2669
Fragments arranged by size:
2669 2641 1485
ApoI r'AATT_y
Cuts at: 0 1239 1944 3654 4230 6490 6795
Size: 1239 705 1710 576 2260 305
Fragments arranged by size:
2260 1710 1239 705 576 305
AseI AT'TA_AT
Cuts at: 0 4607 6795
Size: 4607 2188
AvaI C'yCGr_G
Cuts at: 0 19 1229 3059 4142 4180 4202 6788 6795
Size: 19 1210 1830 1083 38 22 2586 7
Fragments arranged by size:
2586 1830 1210 1083 38 22 19 7
AvrII C'CTAG_G
Cuts at: 0 207 6795
Size: 207 6588
BamHI G'GATC_C
Cuts at: 0 4166 6795
Size: 4166 2629
BanI G'GyrC_C
Cuts at: 0 60 1249 2698 3378 4215 6130 6386 6577
Size: 60 1189 1449 680 837 1915 256 191
Cuts at: 6577 6795
Size: 218
Fragments arranged by size:
1915 1449 1189 837 680 256 218 191
60
BanII G_rGCy'C
Cuts at: 0 20 3066 3226 3268 3691 3864 5190 5407
Size: 20 3046 160 42 423 173 1326 217
Cuts at: 5407 5681 6009 6795
Size: 274 328 786
Fragments arranged by size:
3046 1326 786 423 328 274 217 173
160 42 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 976 4062 5017 6541 6795
Size: 976 3086 955 1524 254
Fragments arranged by size:
3086 1524 976 955 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 850 884 1532 1566 6166 6200 6795
Size: 850 34 648 34 4600 34 595
Fragments arranged by size:
4600 850 648 595 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3070 3201 6795
Size: 3070 131 3594
Fragments arranged by size:
3594 3070 131
BclI T'GATC_A
Cuts at: 0 561 3311 3681 4394 6795
Size: 561 2750 370 713 2401
Fragments arranged by size:
2750 2401 713 561 370
BfrBI ATG'CAT
Cuts at: 0 1523 6795
Size: 1523 5272
BglI GCCn_nnn'nGGC
Cuts at: 0 6614 6795
Size: 6614 181
BglII A'GATC_T
Cuts at: 0 5369 5756 6795
Size: 5369 387 1039
Fragments arranged by size:
5369 1039 387
BlpI GC'TnA_GC
Cuts at: 0 98 6795
Size: 98 6697
Bme1580I G_kGCm'C
Cuts at: 0 20 65 1489 1616 2723 3066 4130 4220
Size: 20 45 1424 127 1107 343 1064 90
Cuts at: 4220 6009 6795
Size: 1789 786
Fragments arranged by size:
1789 1424 1107 1064 786 343 127 90
45 20
BmgBI CAC'GTC
Cuts at: 0 2760 6795
Size: 2760 4035
BmrI ACTGGGnnnn_n'
Cuts at: 0 111 314 1706 4944 5855 6650 6795
Size: 111 203 1392 3238 911 795 145
Fragments arranged by size:
3238 1392 911 795 203 145 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 113 2978 3068 4061 4883 6795
Size: 113 2865 90 993 822 1912
Fragments arranged by size:
2865 1912 993 822 113 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 751 756 2511 2992 3257 3416 3565
Size: 480 271 5 1755 481 265 159 149
Cuts at: 3565 3584 3742 3999 4241 4550 5182 5250 6795
Size: 19 158 257 242 309 632 68 1545
Fragments arranged by size:
1755 1545 632 481 480 309 271 265
257 242 159 158 149 68 19 5
Bpu10I CC'TnA_GC
Cuts at: 0 4 4942 5141 6342 6795
Size: 4 4938 199 1201 453
Fragments arranged by size:
4938 1201 453 199 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1806 6795
Size: 1806 4989
BsaI GGTCTCn'nnnn_
Cuts at: 0 459 1929 2217 2226 3292 4191 5203 5558
Size: 459 1470 288 9 1066 899 1012 355
Cuts at: 5558 6795
Size: 1237
Fragments arranged by size:
1470 1237 1066 1012 899 459 355 288
9
BsaAI yAC'GTr
Cuts at: 0 1001 1490 5509 6546 6795
Size: 1001 489 4019 1037 249
Fragments arranged by size:
4019 1037 1001 489 249
BsaHI Gr'CG_yC
Cuts at: 0 3322 5423 6795
Size: 3322 2101 1372
Fragments arranged by size:
3322 2101 1372
BsaWI w'CCGG_w
Cuts at: 0 5014 6795
Size: 5014 1781
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1839 1869 1975 2005 3312 3342 3846 3876
Size: 1839 30 106 30 1307 30 504 30
Cuts at: 3876 4713 4743 6795
Size: 837 30 2052
Fragments arranged by size:
2052 1839 1307 837 504 106 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 477 809 896 1247 2147 2287 3386 3603
Size: 477 332 87 351 900 140 1099 217
Cuts at: 3603 3794 4130 5546 6795
Size: 191 336 1416 1249
Fragments arranged by size:
1416 1249 1099 900 477 351 336 332
217 191 140 87
BseYI C'CCAG_C
Cuts at: 0 382 571 899 3348 3792 4400 6795
Size: 382 189 328 2449 444 608 2395
Fragments arranged by size:
2449 2395 608 444 382 328 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 847 890 1104 1176 1185 1392 2816
Size: 762 85 43 214 72 9 207 1424
Cuts at: 2816 3678 4883 6447 6576 6795
Size: 862 1205 1564 129 219
Fragments arranged by size:
1564 1424 1205 862 762 219 214 207
129 85 72 43 9
BsiEI CG_ry'CG
Cuts at: 0 792 6795
Size: 792 6003
BsiHKAI G_wGCw'C
Cuts at: 0 1489 2849 3268 3572 3595 3691 4042 4130
Size: 1489 1360 419 304 23 96 351 88
Cuts at: 4130 4187 5407 6795
Size: 57 1220 1388
Fragments arranged by size:
1489 1388 1360 1220 419 351 304 96
88 57 23
BsmI GAATG_Cn'
Cuts at: 0 1896 6795
Size: 1896 4899
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2134 5239 6795
Size: 2134 3105 1556
Fragments arranged by size:
3105 2134 1556
Bsp1286I G_dGCh'C
Cuts at: 0 20 65 1489 1616 2723 2849 3066 3226
Size: 20 45 1424 127 1107 126 217 160
Cuts at: 3226 3268 3572 3595 3691 3864 4042 4130 4187
Size: 42 304 23 96 173 178 88 57
Cuts at: 4187 4220 5190 5407 5681 6009 6795
Size: 33 970 217 274 328 786
Fragments arranged by size:
1424 1107 970 786 328 304 274 217
217 178 173 160 127 126 96 88
57 45 42 33 23 20
BspHI T'CATG_A
Cuts at: 0 1602 6507 6795
Size: 1602 4905 288
Fragments arranged by size:
4905 1602 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 379 541 770 1643 2345 2744 2843 3824
Size: 379 162 229 873 702 399 99 981
Cuts at: 3824 4759 5462 6795
Size: 935 703 1333
Fragments arranged by size:
1333 981 935 873 703 702 399 379
229 162 99
BsrBI CCG'CTC
Cuts at: 0 198 842 6795
Size: 198 644 5953
Fragments arranged by size:
5953 644 198
BsrDI GCAATG_nn'
Cuts at: 0 219 1523 1791 5648 6795
Size: 219 1304 268 3857 1147
Fragments arranged by size:
3857 1304 1147 268 219
BsrFI r'CCGG_y
Cuts at: 0 2803 5521 6795
Size: 2803 2718 1274
Fragments arranged by size:
2803 2718 1274
BsrGI T'GTAC_A
Cuts at: 0 1627 5378 6765 6795
Size: 1627 3751 1387 30
Fragments arranged by size:
3751 1627 1387 30
BssSI C'ACGA_G
Cuts at: 0 1396 3196 3383 5189 6249 6795
Size: 1396 1800 187 1806 1060 546
Fragments arranged by size:
1806 1800 1396 1060 546 187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 671 2859 6795
Size: 671 2188 3936
Fragments arranged by size:
3936 2188 671
BstBI TT'CG_AA
Cuts at: 0 1168 6795
Size: 1168 5627
BstEII G'GTnAC_C
Cuts at: 0 2766 2910 4995 5721 6795
Size: 2766 144 2085 726 1074
Fragments arranged by size:
2766 2085 1074 726 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1372 3894 5568 6397 6795
Size: 1372 2522 1674 829 398
Fragments arranged by size:
2522 1674 1372 829 398
BstYI r'GATC_y
Cuts at: 0 844 2298 4166 5369 5756 6795
Size: 844 1454 1868 1203 387 1039
Fragments arranged by size:
1868 1454 1203 1039 844 387
BstZ17I GTA'TAC
Cuts at: 0 2028 6795
Size: 2028 4767
BtgI C'CryG_G
Cuts at: 0 68 1385 1773 2943 3089 4569 5057 6203
Size: 68 1317 388 1170 146 1480 488 1146
Cuts at: 6203 6795
Size: 592
Fragments arranged by size:
1480 1317 1170 1146 592 488 388 146
68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 393 6795
Size: 393 6402
BtsI GCAGTG_nn'
Cuts at: 0 967 2804 2942 3250 3971 5429 6795
Size: 967 1837 138 308 721 1458 1366
Fragments arranged by size:
1837 1458 1366 967 721 308 138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5418 5453 6795
Size: 5418 35 1342
Fragments arranged by size:
5418 1342 35
DraI TTT'AAA
Cuts at: 0 6324 6795
Size: 6324 471
DrdI GACnn_nn'nnGTC
Cuts at: 0 2764 3519 6795
Size: 2764 755 3276
Fragments arranged by size:
3276 2764 755
EaeI y'GGCC_r
Cuts at: 0 35 789 915 1770 2061 2165 2346 3200
Size: 35 754 126 855 291 104 181 854
Cuts at: 3200 3642 4323 4566 5081 5286 5465 5924 6447
Size: 442 681 243 515 205 179 459 523
Cuts at: 6447 6675 6795
Size: 228 120
Fragments arranged by size:
855 854 754 681 523 515 459 442
291 243 228 205 181 179 126 120
104 35
EagI C'GGCC_G
Cuts at: 0 789 6795
Size: 789 6006
EarI CTCTTCn'nnn_
Cuts at: 0 76 275 1311 1601 3099 5665 6185 6795
Size: 76 199 1036 290 1498 2566 520 610
Fragments arranged by size:
2566 1498 1036 610 520 290 199 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4159 5919 6795
Size: 4159 1760 876
Fragments arranged by size:
4159 1760 876
EcoICRI GAG'CTC
Cuts at: 0 3266 3689 5405 6795
Size: 3266 423 1716 1390
Fragments arranged by size:
3266 1716 1390 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 299 480 751 756 988 2032 2511
Size: 293 6 181 271 5 232 1044 479
Cuts at: 2511 2992 3257 3380 3416 3565 3584 3742 3938
Size: 481 265 123 36 149 19 158 196
Cuts at: 3938 3999 4173 4241 4550 4933 5182 5250 5677
Size: 61 174 68 309 383 249 68 427
Cuts at: 5677 6795
Size: 1118
Fragments arranged by size:
1118 1044 481 479 427 383 309 293
271 265 249 232 196 181 174 158
149 123 68 68 61 36 19 6
5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2727 3585 4049 6795
Size: 2727 858 464 2746
Fragments arranged by size:
2746 2727 858 464
EcoO109I rG'GnC_Cy
Cuts at: 0 16 2733 3133 4356 6006 6795
Size: 16 2717 400 1223 1650 789
Fragments arranged by size:
2717 1650 1223 789 400 16
EcoRI G'AATT_C
Cuts at: 0 4230 6490 6795
Size: 4230 2260 305
Fragments arranged by size:
4230 2260 305
EcoRV GAT'ATC
Cuts at: 0 2378 4984 6795
Size: 2378 2606 1811
Fragments arranged by size:
2606 2378 1811
HaeII r_GCGC'y
Cuts at: 0 2431 4949 6624 6795
Size: 2431 2518 1675 171
Fragments arranged by size:
2518 2431 1675 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1692 1724 1845 1877 2224 2256 2458 2490
Size: 1692 32 121 32 347 32 202 32
Cuts at: 2490 3042 3074 3312 3344 3673 3705 4125 4136
Size: 552 32 238 32 329 32 420 11
Cuts at: 4136 4157 4158 4168 4190 4618 4650 5038 5070
Size: 21 1 10 22 428 32 388 32
Cuts at: 5070 5431 5463 5555 5587 5741 5773 6693 6725
Size: 361 32 92 32 154 32 920 32
Cuts at: 6725 6795
Size: 70
Fragments arranged by size:
1692 920 552 428 420 388 361 347
329 238 202 154 121 92 70 32
32 32 32 32 32 32 32 32
32 32 32 32 22 21 11 10
1
HincII GTy'rAC
Cuts at: 0 1674 2832 6219 6795
Size: 1674 1158 3387 576
Fragments arranged by size:
3387 1674 1158 576
MfeI C'AATT_G
Cuts at: 0 2477 3494 6740 6795
Size: 2477 1017 3246 55
Fragments arranged by size:
3246 2477 1017 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1939 2255 5474 6267 6795
Size: 570 1369 316 3219 793 528
Fragments arranged by size:
3219 1369 793 570 528 316
MscI TGG'CCA
Cuts at: 0 917 2167 3202 4325 5083 5288 5467 6449
Size: 917 1250 1035 1123 758 205 179 982
Cuts at: 6449 6677 6795
Size: 228 118
Fragments arranged by size:
1250 1123 1035 982 917 758 228 205
179 118
MslI CAynn'nnrTG
Cuts at: 0 823 2113 2320 2773 3289 3307 3415 3565
Size: 823 1290 207 453 516 18 108 150
Cuts at: 3565 4190 4549 6148 6381 6395 6795
Size: 625 359 1599 233 14 400
Fragments arranged by size:
1599 1290 823 625 516 453 400 359
233 207 150 108 18 14
MspA1I CmG'CkG
Cuts at: 0 58 659 874 1022 1036 3796 4400 4517
Size: 58 601 215 148 14 2760 604 117
Cuts at: 4517 6612 6795
Size: 2095 183
Fragments arranged by size:
2760 2095 604 601 215 183 148 117
58 14
NcoI C'CATG_G
Cuts at: 0 1385 2943 6795
Size: 1385 1558 3852
Fragments arranged by size:
3852 1558 1385
NsiI A_TGCA'T
Cuts at: 0 1525 6795
Size: 1525 5270
NspI r_CATG'y
Cuts at: 0 492 1523 1620 1634 6470 6599 6795
Size: 492 1031 97 14 4836 129 196
Fragments arranged by size:
4836 1031 492 196 129 97 14
PasI CC'CwG_GG
Cuts at: 0 2729 2905 5806 6795
Size: 2729 176 2901 989
Fragments arranged by size:
2901 2729 989 176
PciI A'CATG_T
Cuts at: 0 488 6466 6795
Size: 488 5978 329
Fragments arranged by size:
5978 488 329
PflMI CCAn_nnn'nTGG
Cuts at: 0 48 359 1886 2777 3337 5228 5284 6002
Size: 48 311 1527 891 560 1891 56 718
Cuts at: 6002 6795
Size: 793
Fragments arranged by size:
1891 1527 891 793 718 560 311 56
48
PfoI T'CCnGG_A
Cuts at: 0 3432 4472 6014 6087 6795
Size: 3432 1040 1542 73 708
Fragments arranged by size:
3432 1542 1040 708 73
PmlI CAC'GTG
Cuts at: 0 1001 1490 5509 6795
Size: 1001 489 4019 1286
Fragments arranged by size:
4019 1286 1001 489
PpuMI rG'GwC_Cy
Cuts at: 0 2733 3133 4356 6795
Size: 2733 400 1223 2439
Fragments arranged by size:
2733 2439 1223 400
PshAI GACnn'nnGTC
Cuts at: 0 1469 6795
Size: 1469 5326
PspOMI G'GGCC_C
Cuts at: 0 16 3062 6005 6795
Size: 16 3046 2943 790
Fragments arranged by size:
3046 2943 790 16
PspXI vC'TCGA_Gb
Cuts at: 0 1229 4142 6795
Size: 1229 2913 2653
Fragments arranged by size:
2913 2653 1229
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5619 5651 6795
Size: 5619 32 1144
Fragments arranged by size:
5619 1144 32
PstI C_TGCA'G
Cuts at: 0 2741 3170 3257 4770 4884 6795
Size: 2741 429 87 1513 114 1911
Fragments arranged by size:
2741 1911 1513 429 114 87
PvuII CAG'CTG
Cuts at: 0 874 1022 1036 3796 4400 6795
Size: 874 148 14 2760 604 2395
Fragments arranged by size:
2760 2395 874 604 148 14
SacI G_AGCT'C
Cuts at: 0 3268 3691 5407 6795
Size: 3268 423 1716 1388
Fragments arranged by size:
3268 1716 1388 423
SalI G'TCGA_C
Cuts at: 0 1672 6795
Size: 1672 5123
SanDI GG'GwC_CC
Cuts at: 0 3133 6795
Size: 3133 3662
SapI GCTCTTCn'nnn_
Cuts at: 0 76 5665 6795
Size: 76 5589 1130
Fragments arranged by size:
5589 1130 76
SexAI A'CCwGG_T
Cuts at: 0 2613 6795
Size: 2613 4182
SfcI C'TryA_G
Cuts at: 0 717 1949 2737 3166 3253 4161 4502 4766
Size: 717 1232 788 429 87 908 341 264
Cuts at: 4766 4880 6795
Size: 114 1915
Fragments arranged by size:
1915 1232 908 788 717 429 341 264
114 87
SmaI CCC'GGG
Cuts at: 0 21 6795
Size: 21 6774
SmlI C'TyrA_G
Cuts at: 0 1229 1821 4142 4202 5113 6795
Size: 1229 592 2321 60 911 1682
Fragments arranged by size:
2321 1682 1229 911 592 60
SrfI GCCC'GGGC
Cuts at: 0 21 6795
Size: 21 6774
StuI AGG'CCT
Cuts at: 0 2009 6119 6795
Size: 2009 4110 676
Fragments arranged by size:
4110 2009 676
StyI C'CwwG_G
Cuts at: 0 207 615 1385 2943 5003 6795
Size: 207 408 770 1558 2060 1792
Fragments arranged by size:
2060 1792 1558 770 408 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 1627 2200 3340 4666 4947 4977 5490
Size: 313 1314 573 1140 1326 281 30 513
Cuts at: 5490 5815 6795
Size: 325 980
Fragments arranged by size:
1326 1314 1140 980 573 513 325 313
281 30
TatI w'GTAC_w
Cuts at: 0 226 299 637 1627 1919 2665 3122 3182
Size: 226 73 338 990 292 746 457 60
Cuts at: 3182 3725 5378 6029 6765 6795
Size: 543 1653 651 736 30
Fragments arranged by size:
1653 990 746 736 651 543 457 338
292 226 73 60 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1491 1523 1839 1871 1975 2007 2237 2269
Size: 1491 32 316 32 104 32 230 32
Cuts at: 2269 3846 3878 4973 5005 5063 5095 6235 6267
Size: 1577 32 1095 32 58 32 1140 32
Cuts at: 6267 6795
Size: 528
Fragments arranged by size:
1577 1491 1140 1095 528 316 230 104
58 32 32 32 32 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 679 6379 6795
Size: 679 5700 416
Fragments arranged by size:
5700 679 416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1051 2205 5484 6283 6795
Size: 1051 1154 3279 799 512
Fragments arranged by size:
3279 1154 1051 799 512
XhoI C'TCGA_G
Cuts at: 0 1229 4142 4202 6795
Size: 1229 2913 60 2593
Fragments arranged by size:
2913 2593 1229 60
XmaI C'CCGG_G
Cuts at: 0 19 6795
Size: 19 6776
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BamHI
BanI BanII BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII MfeI MmeI
MscI MslI MspA1I NcoI NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuII SacI SalI SanDI SapI SexAI SfcI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AloI AscI AsiSI
BaeI BbeI BbvCI BmtI BsaBI BsiWI BspEI BssHII
Bsu36I ClaI DraIII FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PmeI PpiI PsiI PvuI
RsrII SacII SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI Tth111I XmnI ZraI