Restriction map for
pF1KB0009
(Linear) (Six-base) MAPSORT of: KIBB0009.seq Check: 3552 from: 1 to: 4962
KIBB0009.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 605 890 1377 1629 4962
Size: 605 285 487 252 3333
Fragments arranged by size:
3333 605 487 285 252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1963 2711 2971 4267 4499 4738 4962
Size: 733 1230 748 260 1296 232 239 224
Fragments arranged by size:
1296 1230 748 733 260 239 232 224
AfeI AGC'GCT
Cuts at: 0 3387 4962
Size: 3387 1575
AflII C'TTAA_G
Cuts at: 0 3160 4962
Size: 3160 1802
AflIII A'CryG_T
Cuts at: 0 263 1922 4962
Size: 263 1659 3040
Fragments arranged by size:
3040 1659 263
AhdI GACnn_n'nnGTC
Cuts at: 0 4431 4962
Size: 4431 531
AleI CACnn'nnGTG
Cuts at: 0 2834 4962
Size: 2834 2128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2852 4962
Size: 2852 2110
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1459 1491 2254 2286 4962
Size: 1459 32 763 32 2676
Fragments arranged by size:
2676 1459 763 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 753 828 882 1281 1521 1605 2014 2034
Size: 753 75 54 399 240 84 409 20
Cuts at: 2034 2430 2502 2706 3480 4962
Size: 396 72 204 774 1482
Fragments arranged by size:
1482 774 753 409 399 396 240 204
84 75 72 54 20
ApaI G_GGCC'C
Cuts at: 0 1725 4962
Size: 1725 3237
ApaLI G'TGCA_C
Cuts at: 0 592 1510 4962
Size: 592 918 3452
Fragments arranged by size:
3452 918 592
ApoI r'AATT_y
Cuts at: 0 436 2596 3424 3499 3820 4075 4600 4962
Size: 436 2160 828 75 321 255 525 362
Fragments arranged by size:
2160 828 525 436 362 321 255 75
AvaI C'yCGr_G
Cuts at: 0 610 804 1718 1834 2143 3254 3262 4962
Size: 610 194 914 116 309 1111 8 1700
Fragments arranged by size:
1700 1111 914 610 309 194 116 8
AvrII C'CTAG_G
Cuts at: 0 3684 4962
Size: 3684 1278
BamHI G'GATC_C
Cuts at: 0 2714 3407 3935 4962
Size: 2714 693 528 1027
Fragments arranged by size:
2714 1027 693 528
BanI G'GyrC_C
Cuts at: 0 831 3507 4144 4925 4962
Size: 831 2676 637 781 37
Fragments arranged by size:
2676 831 781 637 37
BanII G_rGCy'C
Cuts at: 0 307 667 1462 1719 1725 2153 2630 3296
Size: 307 360 795 257 6 428 477 666
Cuts at: 3296 4289 4962
Size: 993 673
Fragments arranged by size:
993 795 673 666 477 428 360 307
257 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 421 1611 1979 2111 2375 2830 2966
Size: 221 200 1190 368 132 264 455 136
Cuts at: 2966 4962
Size: 1996
Fragments arranged by size:
1996 1190 455 368 264 221 200 136
132
BbvCI CC'TCA_GC
Cuts at: 0 3191 4962
Size: 3191 1771
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 934 968 1221 1255 2878 2912 3725 3759
Size: 934 34 253 34 1623 34 813 34
Cuts at: 3759 4962
Size: 1203
Fragments arranged by size:
1623 1203 934 813 253 34 34 34
34
BclI T'GATC_A
Cuts at: 0 2036 4962
Size: 2036 2926
BfrBI ATG'CAT
Cuts at: 0 4344 4962
Size: 4344 618
BglI GCCn_nnn'nGGC
Cuts at: 0 1421 2989 4962
Size: 1421 1568 1973
Fragments arranged by size:
1973 1568 1421
BglII A'GATC_T
Cuts at: 0 1172 1841 3977 4478 4962
Size: 1172 669 2136 501 484
Fragments arranged by size:
2136 1172 669 501 484
BlpI GC'TnA_GC
Cuts at: 0 1271 3389 4962
Size: 1271 2118 1573
Fragments arranged by size:
2118 1573 1271
Bme1580I G_kGCm'C
Cuts at: 0 596 836 1514 1725 2841 4147 4962
Size: 596 240 678 211 1116 1306 815
Fragments arranged by size:
1306 1116 815 678 596 240 211
BmgBI CAC'GTC
Cuts at: 0 264 1527 4962
Size: 264 1263 3435
Fragments arranged by size:
3435 1263 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 3230 4962
Size: 3230 1732
BmtI G_CTAG'C
Cuts at: 0 634 2458 4962
Size: 634 1824 2504
Fragments arranged by size:
2504 1824 634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 604 985 1033 1582 1712 1885 2668
Size: 589 15 381 48 549 130 173 783
Cuts at: 2668 2857 3049 3473 3694 3736 3826 4036 4153
Size: 189 192 424 221 42 90 210 117
Cuts at: 4153 4321 4387 4958 4962
Size: 168 66 571 4
Fragments arranged by size:
783 589 571 549 424 381 221 210
192 189 173 168 130 117 90 66
48 42 15 4
Bpu10I CC'TnA_GC
Cuts at: 0 1456 2122 3191 4962
Size: 1456 666 1069 1771
Fragments arranged by size:
1771 1456 1069 666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 895 3187 4273 4962
Size: 682 213 2292 1086 689
Fragments arranged by size:
2292 1086 689 682 213
BsaI GGTCTCn'nnnn_
Cuts at: 0 3451 3880 4962
Size: 3451 429 1082
Fragments arranged by size:
3451 1082 429
BsaAI yAC'GTr
Cuts at: 0 1769 4581 4962
Size: 1769 2812 381
Fragments arranged by size:
2812 1769 381
BsaHI Gr'CG_yC
Cuts at: 0 2357 3770 4962
Size: 2357 1413 1192
Fragments arranged by size:
2357 1413 1192
BsaWI w'CCGG_w
Cuts at: 0 3050 4442 4962
Size: 3050 1392 520
Fragments arranged by size:
3050 1392 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2188 2218 2254 2284 2666 2696 4742 4772
Size: 2188 30 36 30 382 30 2046 30
Cuts at: 4772 4840 4870 4962
Size: 68 30 92
Fragments arranged by size:
2188 2046 382 92 68 36 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 523 556 586 742 1852 2278 3481
Size: 322 201 33 30 156 1110 426 1203
Cuts at: 3481 3934 4962
Size: 453 1028
Fragments arranged by size:
1203 1110 1028 453 426 322 201 156
33 30
BseYI C'CCAG_C
Cuts at: 0 497 1181 2152 3087 3732 4563 4962
Size: 497 684 971 935 645 831 399
Fragments arranged by size:
971 935 831 684 645 497 399
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 868 1332 2027 2275 2488 3153 4405
Size: 847 21 464 695 248 213 665 1252
Cuts at: 4405 4962
Size: 557
Fragments arranged by size:
1252 847 695 665 557 464 248 213
21
BsiHKAI G_wGCw'C
Cuts at: 0 596 1514 2630 2798 3296 4289 4580 4962
Size: 596 918 1116 168 498 993 291 382
Fragments arranged by size:
1116 993 918 596 498 382 291 168
BsmI GAATG_Cn'
Cuts at: 0 1092 3192 4962
Size: 1092 2100 1770
Fragments arranged by size:
2100 1770 1092
BsmBI CGTCTCn'nnnn_
Cuts at: 0 442 4962
Size: 442 4520
Bsp1286I G_dGCh'C
Cuts at: 0 307 596 667 836 1462 1514 1719 1725
Size: 307 289 71 169 626 52 205 6
Cuts at: 1725 2153 2630 2798 2841 3296 4147 4289 4580
Size: 428 477 168 43 455 851 142 291
Cuts at: 4580 4962
Size: 382
Fragments arranged by size:
851 626 477 455 428 382 307 291
289 205 169 168 142 71 52 43
6
BspEI T'CCGG_A
Cuts at: 0 4442 4962
Size: 4442 520
BspHI T'CATG_A
Cuts at: 0 195 4962
Size: 195 4767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 605 890 1377 1629 2835 4962
Size: 227 378 285 487 252 1206 2127
Fragments arranged by size:
2127 1206 487 378 285 252 227
BsrBI CCG'CTC
Cuts at: 0 2280 4458 4962
Size: 2280 2178 504
Fragments arranged by size:
2280 2178 504
BsrDI GCAATG_nn'
Cuts at: 0 2727 4859 4962
Size: 2727 2132 103
Fragments arranged by size:
2727 2132 103
BsrFI r'CCGG_y
Cuts at: 0 1141 2878 2963 4962
Size: 1141 1737 85 1999
Fragments arranged by size:
1999 1737 1141 85
BsrGI T'GTAC_A
Cuts at: 0 1632 4962
Size: 1632 3330
BssSI C'ACGA_G
Cuts at: 0 229 2275 2797 4962
Size: 229 2046 522 2165
Fragments arranged by size:
2165 2046 522 229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 753 770 882 1310 1518 2946 4962
Size: 753 17 112 428 208 1428 2016
Fragments arranged by size:
2016 1428 753 428 208 112 17
BstEII G'GTnAC_C
Cuts at: 0 1389 3609 4962
Size: 1389 2220 1353
Fragments arranged by size:
2220 1389 1353
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 1185 3915 4831 4962
Size: 852 333 2730 916 131
Fragments arranged by size:
2730 916 852 333 131
BstYI r'GATC_y
Cuts at: 0 365 1172 1841 2499 2714 3407 3923 3935
Size: 365 807 669 658 215 693 516 12
Cuts at: 3935 3977 4478 4941 4962
Size: 42 501 463 21
Fragments arranged by size:
807 693 669 658 516 501 463 365
215 42 21 12
Bsu36I CC'TnA_GG
Cuts at: 0 3646 4037 4962
Size: 3646 391 925
Fragments arranged by size:
3646 925 391
BtgI C'CryG_G
Cuts at: 0 1118 1662 3590 3614 4962
Size: 1118 544 1928 24 1348
Fragments arranged by size:
1928 1348 1118 544 24
BtsI GCAGTG_nn'
Cuts at: 0 766 816 1320 1514 2668 4050 4962
Size: 766 50 504 194 1154 1382 912
Fragments arranged by size:
1382 1154 912 766 504 194 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 467 502 4962
Size: 467 35 4460
Fragments arranged by size:
4460 467 35
DraI TTT'AAA
Cuts at: 0 4742 4962
Size: 4742 220
DraIII CAC_nnn'GTG
Cuts at: 0 1518 4962
Size: 1518 3444
EaeI y'GGCC_r
Cuts at: 0 242 1484 2813 2880 3554 4181 4962
Size: 242 1242 1329 67 674 627 781
Fragments arranged by size:
1329 1242 781 674 627 242 67
EarI CTCTTCn'nnn_
Cuts at: 0 946 1195 1726 1745 3785 4200 4962
Size: 946 249 531 19 2040 415 762
Fragments arranged by size:
2040 946 762 531 415 249 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 176 3198 4962
Size: 176 3022 1764
Fragments arranged by size:
3022 1764 176
EcoICRI GAG'CTC
Cuts at: 0 2628 3294 4287 4962
Size: 2628 666 993 675
Fragments arranged by size:
2628 993 675 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 604 733 985 1033 1582 1712 1885
Size: 589 15 129 252 48 549 130 173
Cuts at: 1885 1963 2668 2711 2857 2971 3049 3473 3694
Size: 78 705 43 146 114 78 424 221
Cuts at: 3694 3736 3826 4036 4153 4267 4321 4387 4499
Size: 42 90 210 117 114 54 66 112
Cuts at: 4499 4738 4958 4962
Size: 239 220 4
Fragments arranged by size:
705 589 549 424 252 239 221 220
210 173 146 130 129 117 114 114
112 90 78 78 66 54 48 43
42 15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1135 1981 4962
Size: 1135 846 2981
Fragments arranged by size:
2981 1135 846
EcoO109I rG'GnC_Cy
Cuts at: 0 315 471 615 717 1722 2406 3259 3711
Size: 315 156 144 102 1005 684 853 452
Cuts at: 3711 4425 4871 4933 4962
Size: 714 446 62 29
Fragments arranged by size:
1005 853 714 684 452 446 315 156
144 102 62 29
EcoRI G'AATT_C
Cuts at: 0 3499 4962
Size: 3499 1463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 438 1450 2392 3171 4962
Size: 147 291 1012 942 779 1791
Fragments arranged by size:
1791 1012 942 779 291 147
FseI GG_CCGG'CC
Cuts at: 0 2882 4962
Size: 2882 2080
FspI TGC'GCA
Cuts at: 0 904 1873 1891 2314 4962
Size: 904 969 18 423 2648
Fragments arranged by size:
2648 969 904 423 18
FspAI rTGC'GCAy
Cuts at: 0 1873 4962
Size: 1873 3089
HaeII r_GCGC'y
Cuts at: 0 590 2134 2439 3389 3400 4962
Size: 590 1544 305 950 11 1562
Fragments arranged by size:
1562 1544 950 590 305 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 769 801 831 863 2052 2084
Size: 52 32 685 32 30 32 1189 32
Cuts at: 2084 3294 3326 4417 4449 4840 4872 4933 4962
Size: 1210 32 1091 32 391 32 61 29
Fragments arranged by size:
1210 1189 1091 685 391 61 52 32
32 32 32 32 32 32 30 29
HincII GTy'rAC
Cuts at: 0 1098 3693 4962
Size: 1098 2595 1269
Fragments arranged by size:
2595 1269 1098
HindIII A'AGCT_T
Cuts at: 0 92 1909 3538 4962
Size: 92 1817 1629 1424
Fragments arranged by size:
1817 1629 1424 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 1973 2204 2750 2832 4142 4685 4962
Size: 1625 348 231 546 82 1310 543 277
Fragments arranged by size:
1625 1310 546 543 348 277 231 82
MscI TGG'CCA
Cuts at: 0 244 2815 4183 4962
Size: 244 2571 1368 779
Fragments arranged by size:
2571 1368 779 244
MslI CAynn'nnrTG
Cuts at: 0 295 1117 2338 2834 2888 3584 4962
Size: 295 822 1221 496 54 696 1378
Fragments arranged by size:
1378 1221 822 696 496 295 54
MspA1I CmG'CkG
Cuts at: 0 361 501 750 770 933 1185 1449 1602
Size: 361 140 249 20 163 252 264 153
Cuts at: 1602 1905 2427 2784 2790 3552 4170 4962
Size: 303 522 357 6 762 618 792
Fragments arranged by size:
792 762 618 522 361 357 303 264
252 249 163 153 140 20 6
NaeI GCC'GGC
Cuts at: 0 2880 4962
Size: 2880 2082
NcoI C'CATG_G
Cuts at: 0 1662 4962
Size: 1662 3300
NgoMIV G'CCGG_C
Cuts at: 0 2878 4962
Size: 2878 2084
NheI G'CTAG_C
Cuts at: 0 630 2454 4962
Size: 630 1824 2508
Fragments arranged by size:
2508 1824 630
NsiI A_TGCA'T
Cuts at: 0 4346 4962
Size: 4346 616
NspI r_CATG'y
Cuts at: 0 300 901 1926 3234 4141 4348 4356 4962
Size: 300 601 1025 1308 907 207 8 606
Fragments arranged by size:
1308 1025 907 606 601 300 207 8
PasI CC'CwG_GG
Cuts at: 0 1462 3931 4962
Size: 1462 2469 1031
Fragments arranged by size:
2469 1462 1031
PciI A'CATG_T
Cuts at: 0 1922 4962
Size: 1922 3040
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1521 4962
Size: 828 693 3441
Fragments arranged by size:
3441 828 693
PfoI T'CCnGG_A
Cuts at: 0 620 851 1916 2027 2903 3026 4130 4403
Size: 620 231 1065 111 876 123 1104 273
Cuts at: 4403 4962
Size: 559
Fragments arranged by size:
1104 1065 876 620 559 273 231 123
111
PmlI CAC'GTG
Cuts at: 0 4581 4962
Size: 4581 381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 2254 2286 4962
Size: 545 32 1677 32 2676
Fragments arranged by size:
2676 1677 545 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 315 615 2406 3259 3711 4425 4871 4933
Size: 315 300 1791 853 452 714 446 62
Cuts at: 4933 4962
Size: 29
Fragments arranged by size:
1791 853 714 452 446 315 300 62
29
PspOMI G'GGCC_C
Cuts at: 0 1721 4962
Size: 1721 3241
PspXI vC'TCGA_Gb
Cuts at: 0 610 4962
Size: 610 4352
PstI C_TGCA'G
Cuts at: 0 347 761 775 1448 1610 1730 2234 2435
Size: 347 414 14 673 162 120 504 201
Cuts at: 2435 2789 2846 3302 4962
Size: 354 57 456 1660
Fragments arranged by size:
1660 673 504 456 414 354 347 201
162 120 57 14
PvuII CAG'CTG
Cuts at: 0 361 501 933 1185 2784 3552 4962
Size: 361 140 432 252 1599 768 1410
Fragments arranged by size:
1599 1410 768 432 361 252 140
RsrII CG'GwC_CG
Cuts at: 0 6 4962
Size: 6 4956
SacI G_AGCT'C
Cuts at: 0 2630 3296 4289 4962
Size: 2630 666 993 673
Fragments arranged by size:
2630 993 673 666
SanDI GG'GwC_CC
Cuts at: 0 3259 4425 4962
Size: 3259 1166 537
Fragments arranged by size:
3259 1166 537
SapI GCTCTTCn'nnn_
Cuts at: 0 946 1195 4962
Size: 946 249 3767
Fragments arranged by size:
3767 946 249
SbfI CC_TGCA'GG
Cuts at: 0 2846 4962
Size: 2846 2116
SexAI A'CCwGG_T
Cuts at: 0 1514 4962
Size: 1514 3448
SfcI C'TryA_G
Cuts at: 0 343 757 771 1444 1606 1726 2230 2431
Size: 343 414 14 673 162 120 504 201
Cuts at: 2431 2785 2842 3298 3630 4962
Size: 354 57 456 332 1332
Fragments arranged by size:
1332 673 504 456 414 354 343 332
201 162 120 57 14
SmaI CCC'GGG
Cuts at: 0 806 1720 4962
Size: 806 914 3242
Fragments arranged by size:
3242 914 806
SmlI C'TyrA_G
Cuts at: 0 610 697 910 3160 3202 4288 4962
Size: 610 87 213 2250 42 1086 674
Fragments arranged by size:
2250 1086 674 610 213 87 42
SnaBI TAC'GTA
Cuts at: 0 1769 4962
Size: 1769 3193
SphI G_CATG'C
Cuts at: 0 3234 4141 4356 4962
Size: 3234 907 215 606
Fragments arranged by size:
3234 907 606 215
SrfI GCCC'GGGC
Cuts at: 0 1720 4962
Size: 1720 3242
StuI AGG'CCT
Cuts at: 0 1672 3154 3166 4962
Size: 1672 1482 12 1796
Fragments arranged by size:
1796 1672 1482 12
StyI C'CwwG_G
Cuts at: 0 1502 1662 3684 4484 4962
Size: 1502 160 2022 800 478
Fragments arranged by size:
2022 1502 800 478 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1195 2214 3597 4555 4588 4962
Size: 1195 1019 1383 958 33 374
Fragments arranged by size:
1383 1195 1019 958 374 33
TatI w'GTAC_w
Cuts at: 0 1632 2327 2510 3206 4962
Size: 1632 695 183 696 1756
Fragments arranged by size:
1756 1632 696 695 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1180 1212 1285 1317 2188 2220 4742 4774
Size: 1180 32 73 32 871 32 2522 32
Cuts at: 4774 4962
Size: 188
Fragments arranged by size:
2522 1180 871 188 73 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3112 4962
Size: 3112 1850
XbaI T'CTAG_A
Cuts at: 0 2070 4962
Size: 2070 2892
XhoI C'TCGA_G
Cuts at: 0 610 4962
Size: 610 4352
XmaI C'CCGG_G
Cuts at: 0 804 1718 4962
Size: 804 914 3244
Fragments arranged by size:
3244 914 804
XmnI GAAnn'nnTTC
Cuts at: 0 353 1976 2392 4706 4962
Size: 353 1623 416 2314 256
Fragments arranged by size:
2314 1623 416 353 256
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuII RsrII SacI SanDI SapI
SbfI SexAI SfcI SmaI SmlI SnaBI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AscI AseI AsiSI
BaeI BbeI BciVI BplI BsaBI BsiEI BsiWI BssHII
BstBI BstZ17I BtgZI ClaI DrdI EagI EcoRV HpaI
KasI KpnI MfeI MluI NarI NdeI NotI NruI
PacI PmeI PshAI PsiI PsrI PvuI SacII SalI
ScaI SfiI SfoI SgfI SgrAI SpeI SspI SwaI
XcmI ZraI