Restriction map for
pF1KSDB0012
(Linear) (Six-base) MAPSORT of: KIBB0012.seq Check: 3382 from: 1 to: 1431
KIBB0012.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 22 1431
Size: 22 1409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1252 1390 1431
Size: 692 560 138 41
Fragments arranged by size:
692 560 138 41
AlwNI CAG_nnn'CTG
Cuts at: 0 451 958 1431
Size: 451 507 473
Fragments arranged by size:
507 473 451
ApaI G_GGCC'C
Cuts at: 0 63 1431
Size: 63 1368
ApoI r'AATT_y
Cuts at: 0 1024 1431
Size: 1024 407
AvaI C'yCGr_G
Cuts at: 0 516 1431
Size: 516 915
BanI G'GyrC_C
Cuts at: 0 80 438 739 1162 1431
Size: 80 358 301 423 269
Fragments arranged by size:
423 358 301 269 80
BanII G_rGCy'C
Cuts at: 0 63 193 1284 1431
Size: 63 130 1091 147
Fragments arranged by size:
1091 147 130 63
BbeI G_GCGC'C
Cuts at: 0 442 1431
Size: 442 989
BbsI GAAGACnn'nnnn_
Cuts at: 0 1378 1431
Size: 1378 53
BbvCI CC'TCA_GC
Cuts at: 0 719 1094 1431
Size: 719 375 337
Fragments arranged by size:
719 375 337
BciVI GTATCCnnnnn_n'
Cuts at: 0 660 1431
Size: 660 771
BglI GCCn_nnn'nGGC
Cuts at: 0 115 298 409 1431
Size: 115 183 111 1022
Fragments arranged by size:
1022 183 115 111
Bme1580I G_kGCm'C
Cuts at: 0 63 1431
Size: 63 1368
BmrI ACTGGGnnnn_n'
Cuts at: 0 115 361 1431
Size: 115 246 1070
Fragments arranged by size:
1070 246 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1122 1431
Size: 1122 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 523 1431
Size: 301 222 908
Fragments arranged by size:
908 301 222
Bpu10I CC'TnA_GC
Cuts at: 0 719 1094 1431
Size: 719 375 337
Fragments arranged by size:
719 375 337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1431
Size: 241 1190
BsaI GGTCTCn'nnnn_
Cuts at: 0 506 1431
Size: 506 925
BsaHI Gr'CG_yC
Cuts at: 0 267 439 1431
Size: 267 172 992
Fragments arranged by size:
992 267 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 521 551 878 908 1431
Size: 521 30 327 30 523
Fragments arranged by size:
523 521 327 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 193 598 610 613 721 1111 1431
Size: 190 3 405 12 3 108 390 320
Fragments arranged by size:
405 390 320 190 108 12 3 3
BseYI C'CCAG_C
Cuts at: 0 413 444 1431
Size: 413 31 987
Fragments arranged by size:
987 413 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 316 1431
Size: 189 127 1115
Fragments arranged by size:
1115 189 127
BsiEI CG_ry'CG
Cuts at: 0 510 1431
Size: 510 921
BsmBI CGTCTCn'nnnn_
Cuts at: 0 614 1431
Size: 614 817
Bsp1286I G_dGCh'C
Cuts at: 0 63 193 1284 1431
Size: 63 130 1091 147
Fragments arranged by size:
1091 147 130 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 22 101 1431
Size: 22 79 1330
Fragments arranged by size:
1330 79 22
BsrBI CCG'CTC
Cuts at: 0 1309 1431
Size: 1309 122
BsrDI GCAATG_nn'
Cuts at: 0 683 1431
Size: 683 748
BsrGI T'GTAC_A
Cuts at: 0 960 1431
Size: 960 471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 23 212 911 1431
Size: 23 189 699 520
Fragments arranged by size:
699 520 189 23
BtgI C'CryG_G
Cuts at: 0 479 495 1139 1253 1431
Size: 479 16 644 114 178
Fragments arranged by size:
644 479 178 114 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1025 1162 1431
Size: 1025 137 269
Fragments arranged by size:
1025 269 137
BtsI GCAGTG_nn'
Cuts at: 0 308 1431
Size: 308 1123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1208 1243 1431
Size: 1208 35 188
Fragments arranged by size:
1208 188 35
EaeI y'GGCC_r
Cuts at: 0 107 194 389 476 492 1431
Size: 107 87 195 87 16 939
Fragments arranged by size:
939 195 107 87 87 16
EarI CTCTTCn'nnn_
Cuts at: 0 599 707 1003 1431
Size: 599 108 296 428
Fragments arranged by size:
599 428 296 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 114 1431
Size: 114 1317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 523 692 1252 1390 1431
Size: 301 222 169 560 138 41
Fragments arranged by size:
560 301 222 169 138 41
EcoO109I rG'GnC_Cy
Cuts at: 0 500 609 1080 1431
Size: 500 109 471 351
Fragments arranged by size:
500 471 351 109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 1431
Size: 930 501
HaeII r_GCGC'y
Cuts at: 0 442 451 1431
Size: 442 9 980
Fragments arranged by size:
980 442 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1041 1073 1431
Size: 1041 32 358
Fragments arranged by size:
1041 358 32
KasI G'GCGC_C
Cuts at: 0 438 1431
Size: 438 993
MscI TGG'CCA
Cuts at: 0 109 1431
Size: 109 1322
MslI CAynn'nnrTG
Cuts at: 0 974 1431
Size: 974 457
MspA1I CmG'CkG
Cuts at: 0 57 162 1431
Size: 57 105 1269
Fragments arranged by size:
1269 105 57
NarI GG'CG_CC
Cuts at: 0 439 1431
Size: 439 992
NcoI C'CATG_G
Cuts at: 0 1139 1431
Size: 1139 292
NdeI CA'TA_TG
Cuts at: 0 971 1431
Size: 971 460
NspI r_CATG'y
Cuts at: 0 54 1335 1431
Size: 54 1281 96
Fragments arranged by size:
1281 96 54
PasI CC'CwG_GG
Cuts at: 0 1284 1431
Size: 1284 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 736 1431
Size: 736 695
PfoI T'CCnGG_A
Cuts at: 0 140 1431
Size: 140 1291
PpuMI rG'GwC_Cy
Cuts at: 0 500 609 1080 1431
Size: 500 109 471 351
Fragments arranged by size:
500 471 351 109
PspOMI G'GGCC_C
Cuts at: 0 59 1431
Size: 59 1372
SanDI GG'GwC_CC
Cuts at: 0 500 1431
Size: 500 931
SapI GCTCTTCn'nnn_
Cuts at: 0 1003 1431
Size: 1003 428
ScaI AGT'ACT
Cuts at: 0 1265 1431
Size: 1265 166
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 115 409 1431
Size: 115 294 1022
Fragments arranged by size:
1022 294 115
SfoI GGC'GCC
Cuts at: 0 440 1431
Size: 440 991
SmlI C'TyrA_G
Cuts at: 0 256 1431
Size: 256 1175
StyI C'CwwG_G
Cuts at: 0 735 1139 1431
Size: 735 404 292
Fragments arranged by size:
735 404 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 524 1431
Size: 524 907
TatI w'GTAC_w
Cuts at: 0 960 1263 1274 1431
Size: 960 303 11 157
Fragments arranged by size:
960 303 157 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 341 373 521 553 1431
Size: 244 32 65 32 148 32 878
Fragments arranged by size:
878 244 148 65 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1431
Size: 471 960
XmnI GAAnn'nnTTC
Cuts at: 0 940 1148 1431
Size: 940 208 283
Fragments arranged by size:
940 283 208
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstAPI BtgI BtgZI BtsI CspCI EaeI EarI EciI
Eco57MI EcoO109I FalI HaeII Hin4I KasI MscI MslI
MspA1I NarI NcoI NdeI NspI PasI PflMI PfoI
PpuMI PspOMI SanDI SapI ScaI SfiI SfoI SmlI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BclI BfrBI BglII BlpI
BmgBI BmtI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI
BspEI BspHI BsrFI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SbfI SexAI SfcI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI