Restriction map for
pF1KB0013
(Linear) (Six-base) MAPSORT of: KIBB0013.seq Check: 437 from: 1 to: 6471
KIBB0013.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 62 2459 3122 3190 4393 6245 6306 6471
Size: 62 2397 663 68 1203 1852 61 165
Fragments arranged by size:
2397 1852 1203 663 165 68 62 61
AatII G_ACGT'C
Cuts at: 0 140 4079 5609 6004 6471
Size: 140 3939 1530 395 467
Fragments arranged by size:
3939 1530 467 395 140
AccI GT'mk_AC
Cuts at: 0 4229 5105 6471
Size: 4229 876 1366
Fragments arranged by size:
4229 1366 876
Acc65I G'GTAC_C
Cuts at: 0 3896 6471
Size: 3896 2575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1094 1150 1345 1615 2839 3114 3713
Size: 508 586 56 195 270 1224 275 599
Cuts at: 3713 5332 6471
Size: 1619 1139
Fragments arranged by size:
1619 1224 1139 599 586 508 275 270
195 56
AflIII A'CryG_T
Cuts at: 0 527 639 2678 4898 5163 6471
Size: 527 112 2039 2220 265 1308
Fragments arranged by size:
2220 2039 1308 527 265 112
AhdI GACnn_n'nnGTC
Cuts at: 0 1311 2388 6471
Size: 1311 1077 4083
Fragments arranged by size:
4083 1311 1077
AleI CACnn'nnGTG
Cuts at: 0 5305 5626 6471
Size: 5305 321 845
Fragments arranged by size:
5305 845 321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1220 1551 2434 4889 5429 6471
Size: 1220 331 883 2455 540 1042
Fragments arranged by size:
2455 1220 1042 883 540 331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4486 4518 6471
Size: 4486 32 1953
Fragments arranged by size:
4486 1953 32
AlwNI CAG_nnn'CTG
Cuts at: 0 621 1323 1818 2616 3099 3205 3993 4782
Size: 621 702 495 798 483 106 788 789
Cuts at: 4782 5247 5322 6471
Size: 465 75 1149
Fragments arranged by size:
1149 798 789 788 702 621 495 483
465 106 75
ApaI G_GGCC'C
Cuts at: 0 6062 6471
Size: 6062 409
ApaLI G'TGCA_C
Cuts at: 0 253 5020 6471
Size: 253 4767 1451
Fragments arranged by size:
4767 1451 253
ApoI r'AATT_y
Cuts at: 0 892 1054 6471
Size: 892 162 5417
Fragments arranged by size:
5417 892 162
AscI GG'CGCG_CC
Cuts at: 0 6089 6471
Size: 6089 382
AvaI C'yCGr_G
Cuts at: 0 24 463 711 1294 3805 3814 4215 5228
Size: 24 439 248 583 2511 9 401 1013
Cuts at: 5228 5335 5503 5813 6099 6260 6340 6453 6471
Size: 107 168 310 286 161 80 113 18
Fragments arranged by size:
2511 1013 583 439 401 310 286 248
168 161 113 107 80 24 18 9
BamHI G'GATC_C
Cuts at: 0 5906 6423 6471
Size: 5906 517 48
Fragments arranged by size:
5906 517 48
BanI G'GyrC_C
Cuts at: 0 175 398 1867 2155 3065 3110 3284 3896
Size: 175 223 1469 288 910 45 174 612
Cuts at: 3896 5133 6249 6256 6284 6364 6471
Size: 1237 1116 7 28 80 107
Fragments arranged by size:
1469 1237 1116 910 612 288 223 175
174 107 80 45 28 7
BanII G_rGCy'C
Cuts at: 0 2021 2540 3727 4222 4532 5237 5342 5806
Size: 2021 519 1187 495 310 705 105 464
Cuts at: 5806 5974 6062 6106 6377 6471
Size: 168 88 44 271 94
Fragments arranged by size:
2021 1187 705 519 495 464 310 271
168 105 94 88 44
BbeI G_GCGC'C
Cuts at: 0 402 6288 6368 6471
Size: 402 5886 80 103
Fragments arranged by size:
5886 402 103 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 636 1580 2805 2809 3379 3581 3712 4591
Size: 636 944 1225 4 570 202 131 879
Cuts at: 4591 6175 6471
Size: 1584 296
Fragments arranged by size:
1584 1225 944 879 636 570 296 202
131 4
BbvCI CC'TCA_GC
Cuts at: 0 775 1411 3206 4000 6471
Size: 775 636 1795 794 2471
Fragments arranged by size:
2471 1795 794 775 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5 39 2116 2150 6471
Size: 5 34 2077 34 4321
Fragments arranged by size:
4321 2077 34 34 5
BclI T'GATC_A
Cuts at: 0 2237 5639 6471
Size: 2237 3402 832
Fragments arranged by size:
3402 2237 832
BglI GCCn_nnn'nGGC
Cuts at: 0 661 785 2122 2131 3875 4024 4295 5093
Size: 661 124 1337 9 1744 149 271 798
Cuts at: 5093 6265 6471
Size: 1172 206
Fragments arranged by size:
1744 1337 1172 798 661 271 206 149
124 9
BglII A'GATC_T
Cuts at: 0 521 6471
Size: 521 5950
BlpI GC'TnA_GC
Cuts at: 0 3251 3982 4109 6471
Size: 3251 731 127 2362
Fragments arranged by size:
3251 2362 731 127
Bme1580I G_kGCm'C
Cuts at: 0 257 285 1870 2160 3113 3287 3522 4331
Size: 257 28 1585 290 953 174 235 809
Cuts at: 4331 4898 5024 5138 6062 6252 6261 6471
Size: 567 126 114 924 190 9 210
Fragments arranged by size:
1585 953 924 809 567 290 257 235
210 190 174 126 114 28 9
BmgBI CAC'GTC
Cuts at: 0 642 660 1518 3474 6471
Size: 642 18 858 1956 2997
Fragments arranged by size:
2997 1956 858 642 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 2481 2533 3710 5773 6271 6471
Size: 2481 52 1177 2063 498 200
Fragments arranged by size:
2481 2063 1177 498 200 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 741 1548 2766 3357 4862 6471
Size: 741 807 1218 591 1505 1609
Fragments arranged by size:
1609 1505 1218 807 741 591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 856 861 1684 2275 2293 2656 2782
Size: 652 204 5 823 591 18 363 126
Cuts at: 2782 2932 3007 3016 3254 3487 4312 4330 5167
Size: 150 75 9 238 233 825 18 837
Cuts at: 5167 5296 5452 5485 5713 5857 5869 6046 6391
Size: 129 156 33 228 144 12 177 345
Cuts at: 6391 6471
Size: 80
Fragments arranged by size:
837 825 823 652 591 363 345 238
233 228 204 177 156 150 144 129
126 80 75 33 18 18 12 9
5
Bpu10I CC'TnA_GC
Cuts at: 0 679 775 1411 3206 4000 6471
Size: 679 96 636 1795 794 2471
Fragments arranged by size:
2471 1795 794 679 636 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1048 1135 4516 5596 6471
Size: 481 567 87 3381 1080 875
Fragments arranged by size:
3381 1080 875 567 481 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 2632 3693 3802 6471
Size: 2632 1061 109 2669
Fragments arranged by size:
2669 2632 1061 109
BsaAI yAC'GTr
Cuts at: 0 360 530 597 1697 2010 4332 4677 4899
Size: 360 170 67 1100 313 2322 345 222
Cuts at: 4899 5166 5624 6471
Size: 267 458 847
Fragments arranged by size:
2322 1100 847 458 360 345 313 267
222 170 67
BsaBI GATnn'nnATC
Cuts at: 0 5638 6471
Size: 5638 833
BsaHI Gr'CG_yC
Cuts at: 0 137 149 236 399 800 2106 3905 4076
Size: 137 12 87 163 401 1306 1799 171
Cuts at: 4076 4588 5606 6001 6285 6365 6471
Size: 512 1018 395 284 80 106
Fragments arranged by size:
1799 1306 1018 512 401 395 284 171
163 137 106 87 80 12
BsaWI w'CCGG_w
Cuts at: 0 2651 2900 2984 4378 6471
Size: 2651 249 84 1394 2093
Fragments arranged by size:
2651 2093 1394 249 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 926 956 4174 4204 5443 5473
Size: 859 30 37 30 3218 30 1239 30
Cuts at: 5473 5543 5573 6471
Size: 70 30 898
Fragments arranged by size:
3218 1239 898 859 70 37 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 947 1492 1798 2201 3223 3330 3526 4060
Size: 947 545 306 403 1022 107 196 534
Cuts at: 4060 4078 4318 5692 5842 5845 5866 5869 5938
Size: 18 240 1374 150 3 21 3 69
Cuts at: 5938 5995 6043 6471
Size: 57 48 428
Fragments arranged by size:
1374 1022 947 545 534 428 403 306
240 196 150 107 69 57 48 21
18 3 3
BseYI C'CCAG_C
Cuts at: 0 68 621 2051 2729 3424 6245 6471
Size: 68 553 1430 678 695 2821 226
Fragments arranged by size:
2821 1430 695 678 553 226 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 2404 2431 2437 2839 3328 6379 6471
Size: 40 2364 27 6 402 489 3051 92
Fragments arranged by size:
3051 2364 489 402 92 40 27 6
BsiEI CG_ry'CG
Cuts at: 0 2427 3751 6471
Size: 2427 1324 2720
Fragments arranged by size:
2720 2427 1324
BsiHKAI G_wGCw'C
Cuts at: 0 257 296 745 2021 2063 2714 3338 3473
Size: 257 39 449 1276 42 651 624 135
Cuts at: 3473 4532 4889 5024 5237 5438 6471
Size: 1059 357 135 213 201 1033
Fragments arranged by size:
1276 1059 1033 651 624 449 357 257
213 201 135 135 42 39
BsiWI C'GTAC_G
Cuts at: 0 530 6471
Size: 530 5941
BsmI GAATG_Cn'
Cuts at: 0 4320 6471
Size: 4320 2151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2818 2842 3613 4083 5780 5992 6471
Size: 2818 24 771 470 1697 212 479
Fragments arranged by size:
2818 1697 771 479 470 212 24
Bsp1286I G_dGCh'C
Cuts at: 0 257 285 296 745 1870 2021 2063 2160
Size: 257 28 11 449 1125 151 42 97
Cuts at: 2160 2540 2714 3113 3287 3338 3473 3522 3727
Size: 380 174 399 174 51 135 49 205
Cuts at: 3727 4222 4331 4532 4889 4898 5024 5138 5237
Size: 495 109 201 357 9 126 114 99
Cuts at: 5237 5342 5438 5806 5974 6062 6106 6252 6261
Size: 105 96 368 168 88 44 146 9
Cuts at: 6261 6377 6471
Size: 116 94
Fragments arranged by size:
1125 495 449 399 380 368 357 257
205 201 174 174 168 151 146 135
126 116 114 109 105 99 97 96
94 88 51 49 44 42 28 11
9 9
BspEI T'CCGG_A
Cuts at: 0 2651 2900 2984 4378 6471
Size: 2651 249 84 1394 2093
Fragments arranged by size:
2651 2093 1394 249 84
BspHI T'CATG_A
Cuts at: 0 5345 6471
Size: 5345 1126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 1781 2128 2459 3122 3190 3234 4393
Size: 62 1719 347 331 663 68 44 1159
Cuts at: 4393 5011 6245 6306 6359 6471
Size: 618 1234 61 53 112
Fragments arranged by size:
1719 1234 1159 663 618 347 331 112
68 62 61 53 44
BsrBI CCG'CTC
Cuts at: 0 722 2832 3747 3912 6033 6471
Size: 722 2110 915 165 2121 438
Fragments arranged by size:
2121 2110 915 722 438 165
BsrDI GCAATG_nn'
Cuts at: 0 152 6471
Size: 152 6319
BsrFI r'CCGG_y
Cuts at: 0 881 2626 3062 3786 5863 5945 6471
Size: 881 1745 436 724 2077 82 526
Fragments arranged by size:
2077 1745 881 724 526 436 82
BsrGI T'GTAC_A
Cuts at: 0 1454 5183 6329 6471
Size: 1454 3729 1146 142
Fragments arranged by size:
3729 1454 1146 142
BssHII G'CGCG_C
Cuts at: 0 6089 6471
Size: 6089 382
BssSI C'ACGA_G
Cuts at: 0 96 1533 1806 3412 4068 6471
Size: 96 1437 273 1606 656 2403
Fragments arranged by size:
2403 1606 1437 656 273 96
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 2616 2841 2928 4335 4991 5247 5322
Size: 261 2355 225 87 1407 656 256 75
Cuts at: 5322 6256 6471
Size: 934 215
Fragments arranged by size:
2355 1407 934 656 261 256 225 215
87 75
BstEII G'GTnAC_C
Cuts at: 0 1374 3543 6471
Size: 1374 2169 2928
Fragments arranged by size:
2928 2169 1374
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1167 2760 3927 5145 5628 6400 6466 6471
Size: 1167 1593 1167 1218 483 772 66 5
Fragments arranged by size:
1593 1218 1167 1167 772 483 66 5
BstYI r'GATC_y
Cuts at: 0 521 3887 4670 4689 5906 6423 6471
Size: 521 3366 783 19 1217 517 48
Fragments arranged by size:
3366 1217 783 521 517 48 19
Bsu36I CC'TnA_GG
Cuts at: 0 299 3302 6471
Size: 299 3003 3169
Fragments arranged by size:
3169 3003 299
BtgI C'CryG_G
Cuts at: 0 424 1160 1439 1757 2036 2081 3395 3671
Size: 424 736 279 318 279 45 1314 276
Cuts at: 3671 3926 4127 4800 5749 6116 6197 6471
Size: 255 201 673 949 367 81 274
Fragments arranged by size:
1314 949 736 673 424 367 318 279
279 276 274 255 201 81 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4375 5885 6471
Size: 4375 1510 586
Fragments arranged by size:
4375 1510 586
BtsI GCAGTG_nn'
Cuts at: 0 759 1424 2001 2093 4325 4780 4948 5037
Size: 759 665 577 92 2232 455 168 89
Cuts at: 5037 5323 6471
Size: 286 1148
Fragments arranged by size:
2232 1148 759 665 577 455 286 168
92 89
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3816 3851 6471
Size: 3816 35 2620
Fragments arranged by size:
3816 2620 35
DraIII CAC_nnn'GTG
Cuts at: 0 261 4335 5624 6256 6471
Size: 261 4074 1289 632 215
Fragments arranged by size:
4074 1289 632 261 215
DrdI GACnn_nn'nnGTC
Cuts at: 0 2602 3775 6471
Size: 2602 1173 2696
Fragments arranged by size:
2696 2602 1173
EaeI y'GGCC_r
Cuts at: 0 551 653 1217 1442 1823 1966 2039 3735
Size: 551 102 564 225 381 143 73 1696
Cuts at: 3735 3748 3917 3956 4100 4727 4740 5085 5537
Size: 13 169 39 144 627 13 345 452
Cuts at: 5537 6005 6119 6200 6390 6471
Size: 468 114 81 190 81
Fragments arranged by size:
1696 627 564 551 468 452 381 345
225 190 169 144 143 114 102 81
81 73 39 13 13
EagI C'GGCC_G
Cuts at: 0 3748 6471
Size: 3748 2723
EarI CTCTTCn'nnn_
Cuts at: 0 1037 1246 1517 1771 2168 3158 4777 6377
Size: 1037 209 271 254 397 990 1619 1600
Cuts at: 6377 6471
Size: 94
Fragments arranged by size:
1619 1600 1037 990 397 271 254 209
94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 2548 3076 3230 4495 6195 6471
Size: 451 2097 528 154 1265 1700 276
Fragments arranged by size:
2097 1700 1265 528 451 276 154
EcoICRI GAG'CTC
Cuts at: 0 2019 4530 5235 6471
Size: 2019 2511 705 1236
Fragments arranged by size:
2511 2019 1236 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 652 856 861 1094 1150 1345 1615
Size: 508 144 204 5 233 56 195 270
Cuts at: 1615 1684 2275 2293 2656 2782 2839 2932 3007
Size: 69 591 18 363 126 57 93 75
Cuts at: 3007 3016 3114 3254 3487 3713 4312 4330 5167
Size: 9 98 140 233 226 599 18 837
Cuts at: 5167 5296 5332 5452 5485 5713 5857 5869 6046
Size: 129 36 120 33 228 144 12 177
Cuts at: 6046 6391 6471
Size: 345 80
Fragments arranged by size:
837 599 591 508 363 345 270 233
233 228 226 204 195 177 144 144
140 129 126 120 98 93 80 75
69 57 56 36 33 18 18 12
9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 1414 2719 3033 4102 5055 5488 5503
Size: 325 1089 1305 314 1069 953 433 15
Cuts at: 5503 5718 6025 6382 6471
Size: 215 307 357 89
Fragments arranged by size:
1305 1089 1069 953 433 357 325 314
307 215 89 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1125 1551 2274 3323 3357 4362 4974 5193
Size: 1125 426 723 1049 34 1005 612 219
Cuts at: 5193 5790 6047 6058 6059 6471
Size: 597 257 11 1 412
Fragments arranged by size:
1125 1049 1005 723 612 597 426 412
257 219 34 11 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 1155 4836 6471
Size: 522 633 3681 1635
Fragments arranged by size:
3681 1635 633 522
FspI TGC'GCA
Cuts at: 0 2647 6471
Size: 2647 3824
FspAI rTGC'GCAy
Cuts at: 0 2647 6471
Size: 2647 3824
HaeII r_GCGC'y
Cuts at: 0 402 1050 1463 2486 2672 2995 3803 3926
Size: 402 648 413 1023 186 323 808 123
Cuts at: 3926 5248 5536 6288 6368 6471
Size: 1322 288 752 80 103
Fragments arranged by size:
1322 1023 808 752 648 413 402 323
288 186 123 103 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 923 926 955 958 1297 1329 2374 2406
Size: 923 3 29 3 339 32 1045 32
Cuts at: 2406 3087 3119 3622 3654 4174 4206 4222 4254
Size: 681 32 503 32 520 32 16 32
Cuts at: 4254 5113 5145 6471
Size: 859 32 1326
Fragments arranged by size:
1326 1045 923 859 681 520 503 339
32 32 32 32 32 32 32 29
16 3 3
HincII GTy'rAC
Cuts at: 0 1383 4230 5514 6471
Size: 1383 2847 1284 957
Fragments arranged by size:
2847 1383 1284 957
HindIII A'AGCT_T
Cuts at: 0 1390 6471
Size: 1390 5081
KasI G'GCGC_C
Cuts at: 0 398 6284 6364 6471
Size: 398 5886 80 107
Fragments arranged by size:
5886 398 107 80
KpnI G_GTAC'C
Cuts at: 0 3900 6471
Size: 3900 2571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 3722 6471
Size: 1259 2463 2749
Fragments arranged by size:
2749 2463 1259
MscI TGG'CCA
Cuts at: 0 553 1444 1825 3737 3919 4102 4729 4742
Size: 553 891 381 1912 182 183 627 13
Cuts at: 4742 5087 5539 6392 6471
Size: 345 452 853 79
Fragments arranged by size:
1912 891 853 627 553 452 381 345
183 182 79 13
MslI CAynn'nnrTG
Cuts at: 0 547 1165 2026 2086 2410 2629 4351 5305
Size: 547 618 861 60 324 219 1722 954
Cuts at: 5305 5626 6471
Size: 321 845
Fragments arranged by size:
1722 954 861 845 618 547 324 321
219 60
MspA1I CmG'CkG
Cuts at: 0 72 372 621 797 2051 2552 2616 3051
Size: 72 300 249 176 1254 501 64 435
Cuts at: 3051 3271 3894 4159 5409 6471
Size: 220 623 265 1250 1062
Fragments arranged by size:
1254 1250 1062 623 501 435 300 265
249 220 176 72 64
NaeI GCC'GGC
Cuts at: 0 5865 6471
Size: 5865 606
NarI GG'CG_CC
Cuts at: 0 399 6285 6365 6471
Size: 399 5886 80 106
Fragments arranged by size:
5886 399 106 80
NcoI C'CATG_G
Cuts at: 0 1160 1439 2081 3671 5749 6471
Size: 1160 279 642 1590 2078 722
Fragments arranged by size:
2078 1590 1160 722 642 279
NdeI CA'TA_TG
Cuts at: 0 365 6471
Size: 365 6106
NgoMIV G'CCGG_C
Cuts at: 0 5863 6471
Size: 5863 608
NspI r_CATG'y
Cuts at: 0 1965 2634 5523 6471
Size: 1965 669 2889 948
Fragments arranged by size:
2889 1965 948 669
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 3672 3993 6471
Size: 621 3051 321 2478
Fragments arranged by size:
3051 2478 621 321
PfoI T'CCnGG_A
Cuts at: 0 1553 1940 2684 2759 3569 4880 5156 5429
Size: 1553 387 744 75 810 1311 276 273
Cuts at: 5429 5822 6471
Size: 393 649
Fragments arranged by size:
1553 1311 810 744 649 393 387 276
273 75
PmlI CAC'GTG
Cuts at: 0 1697 4332 4677 4899 5166 5624 6471
Size: 1697 2635 345 222 267 458 847
Fragments arranged by size:
2635 1697 847 458 345 267 222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5977 6009 6471
Size: 5977 32 462
Fragments arranged by size:
5977 462 32
PpuMI rG'GwC_Cy
Cuts at: 0 1125 1551 2274 3323 5790 6471
Size: 1125 426 723 1049 2467 681
Fragments arranged by size:
2467 1125 1049 723 681 426
PshAI GACnn'nnGTC
Cuts at: 0 2809 6471
Size: 2809 3662
PsiI TTA'TAA
Cuts at: 0 1247 6471
Size: 1247 5224
PspOMI G'GGCC_C
Cuts at: 0 6058 6471
Size: 6058 413
PspXI vC'TCGA_Gb
Cuts at: 0 5503 6471
Size: 5503 968
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1942 1974 6471
Size: 1942 32 4497
Fragments arranged by size:
4497 1942 32
PstI C_TGCA'G
Cuts at: 0 329 2139 2612 2740 2846 2891 2933 3095
Size: 329 1810 473 128 106 45 42 162
Cuts at: 3095 3119 4119 4404 4987 5252 5703 6471
Size: 24 1000 285 583 265 451 768
Fragments arranged by size:
1810 1000 768 583 473 451 329 285
265 162 128 106 45 42 24
PvuII CAG'CTG
Cuts at: 0 72 372 621 2616 4159 5409 6471
Size: 72 300 249 1995 1543 1250 1062
Fragments arranged by size:
1995 1543 1250 1062 300 249 72
SacI G_AGCT'C
Cuts at: 0 2021 4532 5237 6471
Size: 2021 2511 705 1234
Fragments arranged by size:
2511 2021 1234 705
SalI G'TCGA_C
Cuts at: 0 4228 6471
Size: 4228 2243
SapI GCTCTTCn'nnn_
Cuts at: 0 1037 2168 6471
Size: 1037 1131 4303
Fragments arranged by size:
4303 1131 1037
SbfI CC_TGCA'GG
Cuts at: 0 2740 2846 6471
Size: 2740 106 3625
Fragments arranged by size:
3625 2740 106
ScaI AGT'ACT
Cuts at: 0 1858 6471
Size: 1858 4613
SexAI A'CCwGG_T
Cuts at: 0 4757 4913 6471
Size: 4757 156 1558
Fragments arranged by size:
4757 1558 156
SfcI C'TryA_G
Cuts at: 0 325 1259 1404 2135 2608 2736 2842 2887
Size: 325 934 145 731 473 128 106 45
Cuts at: 2887 2929 3091 3115 4115 4400 4983 5248 5699
Size: 42 162 24 1000 285 583 265 451
Cuts at: 5699 6471
Size: 772
Fragments arranged by size:
1000 934 772 731 583 473 451 325
285 265 162 145 128 106 45 42
24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 661 2122 4024 6471
Size: 661 1461 1902 2447
Fragments arranged by size:
2447 1902 1461 661
SfoI GGC'GCC
Cuts at: 0 400 6286 6366 6471
Size: 400 5886 80 105
Fragments arranged by size:
5886 400 105 80
SmaI CCC'GGG
Cuts at: 0 4217 5815 6262 6342 6471
Size: 4217 1598 447 80 129
Fragments arranged by size:
4217 1598 447 129 80
SmlI C'TyrA_G
Cuts at: 0 496 1063 1150 4531 5503 5611 6471
Size: 496 567 87 3381 972 108 860
Fragments arranged by size:
3381 972 860 567 496 108 87
SphI G_CATG'C
Cuts at: 0 2634 6471
Size: 2634 3837
StuI AGG'CCT
Cuts at: 0 94 2962 3028 3732 5128 6224 6471
Size: 94 2868 66 704 1396 1096 247
Fragments arranged by size:
2868 1396 1096 704 247 94 66
StyI C'CwwG_G
Cuts at: 0 116 1160 1439 2081 2720 2807 3671 3844
Size: 116 1044 279 642 639 87 864 173
Cuts at: 3844 4103 4271 4418 5749 5965 6471
Size: 259 168 147 1331 216 506
Fragments arranged by size:
1331 1044 864 642 639 506 279 259
216 173 168 147 116 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 216 808 2183 3562 6471
Size: 216 592 1375 1379 2909
Fragments arranged by size:
2909 1379 1375 592 216
TatI w'GTAC_w
Cuts at: 0 1454 1856 2675 4649 5183 6329 6471
Size: 1454 402 819 1974 534 1146 142
Fragments arranged by size:
1974 1454 1146 819 534 402 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3484 3516 5543 5575 6471
Size: 3484 32 2027 32 896
Fragments arranged by size:
3484 2027 896 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1723 3221 6471
Size: 1723 1498 3250
Fragments arranged by size:
3250 1723 1498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 1167 1227 1551 3762 6471
Size: 165 1002 60 324 2211 2709
Fragments arranged by size:
2709 2211 1002 324 165 60
XhoI C'TCGA_G
Cuts at: 0 5503 6471
Size: 5503 968
XmaI C'CCGG_G
Cuts at: 0 4215 5813 6260 6340 6471
Size: 4215 1598 447 80 131
Fragments arranged by size:
4215 1598 447 131 80
XmnI GAAnn'nnTTC
Cuts at: 0 3605 4819 6471
Size: 3605 1214 1652
Fragments arranged by size:
3605 1652 1214
ZraI GAC'GTC
Cuts at: 0 138 4077 5607 6002 6471
Size: 138 3939 1530 395 469
Fragments arranged by size:
3939 1530 469 395 138
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AseI AsiSI AvrII BaeI
BciVI BfrBI BmtI BstBI BstZ17I ClaI DraI EcoRI
EcoRV FseI HpaI MfeI MluI NheI NotI NruI
NsiI PacI PasI PciI PmeI PvuI RsrII SacII
SanDI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XbaI