Restriction map for
pF1KSDB0014
(Linear) (Six-base) MAPSORT of: KIBB0014.seq Check: 4866 from: 1 to: 5880
KIBB0014.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2505 2807 3825 3987 4587 5538 5880
Size: 2505 302 1018 162 600 951 342
Fragments arranged by size:
2505 1018 951 600 342 302 162
AatII G_ACGT'C
Cuts at: 0 2345 5880
Size: 2345 3535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 883 1024 1705 2350 2479 3535 4876
Size: 413 470 141 681 645 129 1056 1341
Cuts at: 4876 5044 5275 5551 5635 5880
Size: 168 231 276 84 245
Fragments arranged by size:
1341 1056 681 645 470 413 276 245
231 168 141 129 84
AfeI AGC'GCT
Cuts at: 0 2788 4813 5880
Size: 2788 2025 1067
Fragments arranged by size:
2788 2025 1067
AflII C'TTAA_G
Cuts at: 0 5368 5880
Size: 5368 512
AflIII A'CryG_T
Cuts at: 0 918 950 5880
Size: 918 32 4930
Fragments arranged by size:
4930 918 32
AhdI GACnn_n'nnGTC
Cuts at: 0 1086 3360 3882 5880
Size: 1086 2274 522 1998
Fragments arranged by size:
2274 1998 1086 522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2603 5524 5880
Size: 2603 2921 356
Fragments arranged by size:
2921 2603 356
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 1582 1614 5880
Size: 803 32 747 32 4266
Fragments arranged by size:
4266 803 747 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 570 633 2799 2883 3135 3663 3768 3945
Size: 570 63 2166 84 252 528 105 177
Cuts at: 3945 3972 4263 4347 5346 5880
Size: 27 291 84 999 534
Fragments arranged by size:
2166 999 570 534 528 291 252 177
105 84 84 63 27
ApaI G_GGCC'C
Cuts at: 0 2712 4452 4587 5880
Size: 2712 1740 135 1293
Fragments arranged by size:
2712 1740 1293 135
ApaLI G'TGCA_C
Cuts at: 0 513 5880
Size: 513 5367
ApoI r'AATT_y
Cuts at: 0 709 4014 5880
Size: 709 3305 1866
Fragments arranged by size:
3305 1866 709
AvaI C'yCGr_G
Cuts at: 0 931 1563 1897 2092 3643 4412 4445 5434
Size: 931 632 334 195 1551 769 33 989
Cuts at: 5434 5880
Size: 446
Fragments arranged by size:
1551 989 931 769 632 446 334 195
33
BamHI G'GATC_C
Cuts at: 0 1346 1767 5880
Size: 1346 421 4113
Fragments arranged by size:
4113 1346 421
BanI G'GyrC_C
Cuts at: 0 99 1330 1651 4133 5812 5880
Size: 99 1231 321 2482 1679 68
Fragments arranged by size:
2482 1679 1231 321 99 68
BanII G_rGCy'C
Cuts at: 0 266 326 2246 2705 2712 3200 3221 3254
Size: 266 60 1920 459 7 488 21 33
Cuts at: 3254 3482 4452 4534 4587 5154 5880
Size: 228 970 82 53 567 726
Fragments arranged by size:
1920 970 726 567 488 459 266 228
82 60 53 33 21 7
BbeI G_GCGC'C
Cuts at: 0 1334 5816 5880
Size: 1334 4482 64
Fragments arranged by size:
4482 1334 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 871 1050 2130 2274 2999 3148 3359 3526
Size: 871 179 1080 144 725 149 211 167
Cuts at: 3526 4243 4366 4618 5880
Size: 717 123 252 1262
Fragments arranged by size:
1262 1080 871 725 717 252 211 179
167 149 144 123
BbvCI CC'TCA_GC
Cuts at: 0 1981 2696 3178 4148 4400 5861 5880
Size: 1981 715 482 970 252 1461 19
Fragments arranged by size:
1981 1461 970 715 482 252 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 95 2668 2702 5880
Size: 61 34 2573 34 3178
Fragments arranged by size:
3178 2573 61 34 34
BclI T'GATC_A
Cuts at: 0 3083 5240 5880
Size: 3083 2157 640
Fragments arranged by size:
3083 2157 640
BglI GCCn_nnn'nGGC
Cuts at: 0 76 1243 1565 2117 3265 4096 4696 5437
Size: 76 1167 322 552 1148 831 600 741
Cuts at: 5437 5599 5662 5880
Size: 162 63 218
Fragments arranged by size:
1167 1148 831 741 600 552 322 218
162 76 63
BglII A'GATC_T
Cuts at: 0 1370 2744 3335 5880
Size: 1370 1374 591 2545
Fragments arranged by size:
2545 1374 1370 591
BlpI GC'TnA_GC
Cuts at: 0 3973 5880
Size: 3973 1907
Bme1580I G_kGCm'C
Cuts at: 0 517 1654 2712 4452 4587 5398 5792 5880
Size: 517 1137 1058 1740 135 811 394 88
Fragments arranged by size:
1740 1137 1058 811 517 394 135 88
BmgBI CAC'GTC
Cuts at: 0 921 5880
Size: 921 4959
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 4695 5880
Size: 84 4611 1185
Fragments arranged by size:
4611 1185 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1183 3183 5880
Size: 1183 2000 2697
Fragments arranged by size:
2697 2000 1183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 640 673 872 1480 2260 2332 2419
Size: 548 92 33 199 608 780 72 87
Cuts at: 2419 2650 2875 2911 2947 2968 3157 3178 3184
Size: 231 225 36 36 21 189 21 6
Cuts at: 3184 3238 3331 3379 3547 3634 3685 3937 4054
Size: 54 93 48 168 87 51 252 117
Cuts at: 4054 4084 4153 4201 4252 4336 4474 4504 4588
Size: 30 69 48 51 84 138 30 84
Cuts at: 4588 4651 4813 4864 4885 5200 5221 5233 5323
Size: 63 162 51 21 315 21 12 90
Cuts at: 5323 5476 5656 5719 5880
Size: 153 180 63 161
Fragments arranged by size:
780 608 548 315 252 231 225 199
189 180 168 162 161 153 138 117
93 92 90 87 87 84 84 72
69 63 63 54 51 51 51 48
48 36 36 33 30 30 21 21
21 21 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 1981 2696 3178 3481 3979 4148 4189 4400
Size: 1981 715 482 303 498 169 41 211
Cuts at: 4400 5761 5861 5880
Size: 1361 100 19
Fragments arranged by size:
1981 1361 715 498 482 303 211 169
100 41 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 286 2863 3046 3205 3292 3976 5608
Size: 121 165 2577 183 159 87 684 1632
Cuts at: 5608 5880
Size: 272
Fragments arranged by size:
2577 1632 684 272 183 165 159 121
87
BsaI GGTCTCn'nnnn_
Cuts at: 0 955 1879 2318 4417 5470 5880
Size: 955 924 439 2099 1053 410
Fragments arranged by size:
2099 1053 955 924 439 410
BsaAI yAC'GTr
Cuts at: 0 269 579 5880
Size: 269 310 5301
Fragments arranged by size:
5301 310 269
BsaBI GATnn'nnATC
Cuts at: 0 23 5880
Size: 23 5857
BsaHI Gr'CG_yC
Cuts at: 0 583 1087 1331 2342 5175 5705 5813 5880
Size: 583 504 244 1011 2833 530 108 67
Fragments arranged by size:
2833 1011 583 530 504 244 108 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 1582 1612 3469 3499 3782 3812
Size: 679 30 873 30 1857 30 283 30
Cuts at: 3812 5404 5434 5880
Size: 1592 30 446
Fragments arranged by size:
1857 1592 873 679 446 283 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 684 1015 1483 1603 2329 2332 2545
Size: 157 527 331 468 120 726 3 213
Cuts at: 2545 2548 2566 2569 2713 2788 2791 2794 2797
Size: 3 18 3 144 75 3 3 3
Cuts at: 2797 2860 2872 2875 2878 2944 2947 3046 3049
Size: 63 12 3 3 66 3 99 3
Cuts at: 3049 3130 3259 3262 3544 3547 3610 3970 4051
Size: 81 129 3 282 3 63 360 81
Cuts at: 4051 4054 4057 4060 4165 4219 4369 4399 4618
Size: 3 3 3 105 54 150 30 219
Cuts at: 4618 4756 4999 5179 5218 5221 5230 5233 5473
Size: 138 243 180 39 3 9 3 240
Cuts at: 5473 5653 5665 5880
Size: 180 12 215
Fragments arranged by size:
726 527 468 360 331 282 243 240
219 215 213 180 180 157 150 144
138 129 120 105 99 81 81 75
66 63 63 54 39 30 18 12
12 9 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 3470 3923 5096 5195 5657 5880
Size: 3470 453 1173 99 462 223
Fragments arranged by size:
3470 1173 462 453 223 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 5880
Size: 277 5603
BsiEI CG_ry'CG
Cuts at: 0 4395 5250 5720 5880
Size: 4395 855 470 160
Fragments arranged by size:
4395 855 470 160
BsiHKAI G_wGCw'C
Cuts at: 0 266 517 860 2225 2246 2705 3200 3221
Size: 266 251 343 1365 21 459 495 21
Cuts at: 3221 3254 3482 3734 3985 4534 5880
Size: 33 228 252 251 549 1346
Fragments arranged by size:
1365 1346 549 495 459 343 266 252
251 251 228 33 21 21
BsiWI C'GTAC_G
Cuts at: 0 575 5880
Size: 575 5305
BsmI GAATG_Cn'
Cuts at: 0 1568 5337 5880
Size: 1568 3769 543
Fragments arranged by size:
3769 1568 543
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2608 5707 5880
Size: 2608 3099 173
Fragments arranged by size:
3099 2608 173
Bsp1286I G_dGCh'C
Cuts at: 0 266 326 517 860 1654 2225 2246 2705
Size: 266 60 191 343 794 571 21 459
Cuts at: 2705 2712 3200 3221 3254 3482 3734 3985 4452
Size: 7 488 21 33 228 252 251 467
Cuts at: 4452 4534 4587 5154 5398 5792 5880
Size: 82 53 567 244 394 88
Fragments arranged by size:
794 571 567 488 467 459 394 343
266 252 251 244 228 191 88 82
60 53 33 21 21 7
BspHI T'CATG_A
Cuts at: 0 2225 5880
Size: 2225 3655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 1529 2505 2807 3762 3825 3987 4280
Size: 386 1143 976 302 955 63 162 293
Cuts at: 4280 4587 4691 4724 5276 5538 5783 5880
Size: 307 104 33 552 262 245 97
Fragments arranged by size:
1143 976 955 552 386 307 302 293
262 245 162 104 97 63 33
BsrBI CCG'CTC
Cuts at: 0 624 1071 1269 3675 4488 4710 5106 5562
Size: 624 447 198 2406 813 222 396 456
Cuts at: 5562 5880
Size: 318
Fragments arranged by size:
2406 813 624 456 447 396 318 222
198
BsrDI GCAATG_nn'
Cuts at: 0 1355 3086 5880
Size: 1355 1731 2794
Fragments arranged by size:
2794 1731 1355
BsrFI r'CCGG_y
Cuts at: 0 1360 1720 1873 2038 2293 2522 3962 5249
Size: 1360 360 153 165 255 229 1440 1287
Cuts at: 5249 5880
Size: 631
Fragments arranged by size:
1440 1360 1287 631 360 255 229 165
153
BsrGI T'GTAC_A
Cuts at: 0 410 5880
Size: 410 5470
BssSI C'ACGA_G
Cuts at: 0 430 785 2026 2059 3547 4201 4561 4979
Size: 430 355 1241 33 1488 654 360 418
Cuts at: 4979 5880
Size: 901
Fragments arranged by size:
1488 1241 901 654 430 418 360 355
33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 633 2061 2883 3768 5880
Size: 435 198 1428 822 885 2112
Fragments arranged by size:
2112 1428 885 822 435 198
BstEII G'GTnAC_C
Cuts at: 0 2898 3822 5880
Size: 2898 924 2058
Fragments arranged by size:
2898 2058 924
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 1971 2133 5880
Size: 90 1881 162 3747
Fragments arranged by size:
3747 1881 162 90
BstYI r'GATC_y
Cuts at: 0 1346 1370 1767 2165 2744 3314 3335 3557
Size: 1346 24 397 398 579 570 21 222
Cuts at: 3557 4988 5880
Size: 1431 892
Fragments arranged by size:
1431 1346 892 579 570 398 397 222
24 21
BtgI C'CryG_G
Cuts at: 0 965 3590 4463 5072 5880
Size: 965 2625 873 609 808
Fragments arranged by size:
2625 965 873 808 609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1770 5175 5880
Size: 1770 3405 705
Fragments arranged by size:
3405 1770 705
BtsI GCAGTG_nn'
Cuts at: 0 1792 1891 2482 4829 5880
Size: 1792 99 591 2347 1051
Fragments arranged by size:
2347 1792 1051 591 99
DrdI GACnn_nn'nnGTC
Cuts at: 0 1819 2622 5880
Size: 1819 803 3258
Fragments arranged by size:
3258 1819 803
EaeI y'GGCC_r
Cuts at: 0 1250 1871 1961 2268 2381 2543 2969 3233
Size: 1250 621 90 307 113 162 426 264
Cuts at: 3233 3266 3686 3812 4097 4214 4826 4883 5717
Size: 33 420 126 285 117 612 57 834
Cuts at: 5717 5880
Size: 163
Fragments arranged by size:
1250 834 621 612 426 420 307 285
264 163 162 126 117 113 90 57
33
EagI C'GGCC_G
Cuts at: 0 5717 5880
Size: 5717 163
EarI CTCTTCn'nnn_
Cuts at: 0 149 389 418 992 1441 2494 2735 3023
Size: 149 240 29 574 449 1053 241 288
Cuts at: 3023 3092 3272 3410 4153 4619 5020 5642 5828
Size: 69 180 138 743 466 401 622 186
Cuts at: 5828 5880
Size: 52
Fragments arranged by size:
1053 743 622 574 466 449 401 288
241 240 186 180 149 138 69 52
29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 784 2821 3619 4366 4420 4492 5880
Size: 784 2037 798 747 54 72 1388
Fragments arranged by size:
2037 1388 798 784 747 72 54
EcoICRI GAG'CTC
Cuts at: 0 264 2244 2703 3198 3219 3252 3480 4532
Size: 264 1980 459 495 21 33 228 1052
Cuts at: 4532 5880
Size: 1348
Fragments arranged by size:
1980 1348 1052 495 459 264 228 33
21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 548 640 673 872 883 1024 1480
Size: 413 135 92 33 199 11 141 456
Cuts at: 1480 1705 2260 2332 2350 2419 2479 2650 2875
Size: 225 555 72 18 69 60 171 225
Cuts at: 2875 2911 2947 2968 3157 3178 3184 3238 3331
Size: 36 36 21 189 21 6 54 93
Cuts at: 3331 3379 3535 3547 3634 3685 3937 4054 4084
Size: 48 156 12 87 51 252 117 30
Cuts at: 4084 4153 4201 4252 4336 4474 4504 4588 4651
Size: 69 48 51 84 138 30 84 63
Cuts at: 4651 4813 4864 4876 4885 5044 5200 5221 5233
Size: 162 51 12 9 159 156 21 12
Cuts at: 5233 5275 5323 5476 5551 5635 5656 5719 5880
Size: 42 48 153 75 84 21 63 161
Fragments arranged by size:
555 456 413 252 225 225 199 189
171 162 161 159 156 156 153 141
138 135 117 93 92 87 84 84
84 75 72 69 69 63 63 60
54 51 51 51 48 48 48 42
36 36 33 30 30 21 21 21
21 18 12 12 12 11 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 1462 1864 2314 2367 3529 3646 4351
Size: 139 1323 402 450 53 1162 117 705
Cuts at: 4351 4996 5880
Size: 645 884
Fragments arranged by size:
1323 1162 884 705 645 450 402 139
117 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1558 1901 2430 3405 4176 4185 4449 4521
Size: 1558 343 529 975 771 9 264 72
Cuts at: 4521 4584 4713 4860 5772 5880
Size: 63 129 147 912 108
Fragments arranged by size:
1558 975 912 771 529 343 264 147
129 108 72 63 9
EcoRI G'AATT_C
Cuts at: 0 4014 5880
Size: 4014 1866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 4035 5880
Size: 135 3900 1845
Fragments arranged by size:
3900 1845 135
FspI TGC'GCA
Cuts at: 0 1294 3802 5880
Size: 1294 2508 2078
Fragments arranged by size:
2508 2078 1294
FspAI rTGC'GCAy
Cuts at: 0 3802 5880
Size: 3802 2078
HaeII r_GCGC'y
Cuts at: 0 1334 2790 2873 3105 3430 4299 4815 5091
Size: 1334 1456 83 232 325 869 516 276
Cuts at: 5091 5490 5816 5880
Size: 399 326 64
Fragments arranged by size:
1456 1334 869 516 399 326 325 276
232 83 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 426 458 679 711 1072 1104 1362 1369
Size: 426 32 221 32 361 32 258 7
Cuts at: 1369 1394 1401 2150 2157 2182 2189 3025 3057
Size: 25 7 749 7 25 7 836 32
Cuts at: 3057 3313 3345 3346 3378 3469 3501 3868 3900
Size: 256 32 1 32 91 32 367 32
Cuts at: 3900 4416 4448 5587 5619 5880
Size: 516 32 1139 32 261
Fragments arranged by size:
1139 836 749 516 426 367 361 261
258 256 221 91 32 32 32 32
32 32 32 32 32 32 25 25
7 7 7 7 1
HincII GTy'rAC
Cuts at: 0 4680 5880
Size: 4680 1200
KasI G'GCGC_C
Cuts at: 0 1330 5812 5880
Size: 1330 4482 68
Fragments arranged by size:
4482 1330 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 729 860 1862 3422 3899 4652 5494
Size: 131 598 131 1002 1560 477 753 842
Cuts at: 5494 5852 5880
Size: 358 28
Fragments arranged by size:
1560 1002 842 753 598 477 358 131
131 28
MscI TGG'CCA
Cuts at: 0 1252 1963 2270 2383 2545 2971 3235 3268
Size: 1252 711 307 113 162 426 264 33
Cuts at: 3268 3688 5880
Size: 420 2192
Fragments arranged by size:
2192 1252 711 426 420 307 264 162
113 33
MslI CAynn'nnrTG
Cuts at: 0 449 1183 2608 4513 5880
Size: 449 734 1425 1905 1367
Fragments arranged by size:
1905 1425 1367 734 449
MspA1I CmG'CkG
Cuts at: 0 60 630 1108 1280 1398 1683 1959 2009
Size: 60 570 478 172 118 285 276 50
Cuts at: 2009 2073 2439 2508 2583 2652 2853 2889 3231
Size: 64 366 69 75 69 201 36 342
Cuts at: 3231 3466 3612 3759 3927 4032 4197 4620 4758
Size: 235 146 147 168 105 165 423 138
Cuts at: 4758 4917 5074 5141 5199 5346 5454 5466 5634
Size: 159 157 67 58 147 108 12 168
Cuts at: 5634 5661 5880
Size: 27 219
Fragments arranged by size:
570 478 423 366 342 285 276 235
219 201 172 168 168 165 159 157
147 147 146 138 118 108 105 75
69 69 67 64 60 58 50 36
27 12
NaeI GCC'GGC
Cuts at: 0 1362 1722 1875 2040 2524 5880
Size: 1362 360 153 165 484 3356
Fragments arranged by size:
3356 1362 484 360 165 153
NarI GG'CG_CC
Cuts at: 0 1331 5813 5880
Size: 1331 4482 67
Fragments arranged by size:
4482 1331 67
NcoI C'CATG_G
Cuts at: 0 965 4463 5880
Size: 965 3498 1417
Fragments arranged by size:
3498 1417 965
NgoMIV G'CCGG_C
Cuts at: 0 1360 1720 1873 2038 2522 5880
Size: 1360 360 153 165 484 3358
Fragments arranged by size:
3358 1360 484 360 165 153
NspI r_CATG'y
Cuts at: 0 954 1881 1926 4953 5880
Size: 954 927 45 3027 927
Fragments arranged by size:
3027 954 927 927 45
PciI A'CATG_T
Cuts at: 0 950 5880
Size: 950 4930
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 1131 4347 4566 5880
Size: 570 561 3216 219 1314
Fragments arranged by size:
3216 1314 570 561 219
PfoI T'CCnGG_A
Cuts at: 0 650 956 1349 1457 2141 2834 2945 3317
Size: 650 306 393 108 684 693 111 372
Cuts at: 3317 3347 3376 3560 4022 5880
Size: 30 29 184 462 1858
Fragments arranged by size:
1858 693 684 650 462 393 372 306
184 111 108 30 29
PmlI CAC'GTG
Cuts at: 0 269 5880
Size: 269 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1258 1290 1582 1614 5880
Size: 1258 32 292 32 4266
Fragments arranged by size:
4266 1258 292 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2430 3405 4176 4521 4713 4860 5772 5880
Size: 2430 975 771 345 192 147 912 108
Fragments arranged by size:
2430 975 912 771 345 192 147 108
PspOMI G'GGCC_C
Cuts at: 0 2708 4448 4583 5880
Size: 2708 1740 135 1297
Fragments arranged by size:
2708 1740 1297 135
PspXI vC'TCGA_Gb
Cuts at: 0 5434 5880
Size: 5434 446
PstI C_TGCA'G
Cuts at: 0 638 1430 1526 2371 2516 2624 2657 2804
Size: 638 792 96 845 145 108 33 147
Cuts at: 2804 2888 3713 3764 3773 3836 3932 3953 4256
Size: 84 825 51 9 63 96 21 303
Cuts at: 4256 4646 4688 4721 4931 5273 5384 5549 5690
Size: 390 42 33 210 342 111 165 141
Cuts at: 5690 5880
Size: 190
Fragments arranged by size:
845 825 792 638 390 342 303 210
190 165 147 145 141 111 108 96
96 84 63 51 42 33 33 21
9
PvuII CAG'CTG
Cuts at: 0 630 1108 1398 1683 1959 2073 2583 2652
Size: 630 478 290 285 276 114 510 69
Cuts at: 2652 2853 2889 3231 3466 3612 3759 3927 4032
Size: 201 36 342 235 146 147 168 105
Cuts at: 4032 4620 4758 4917 5199 5346 5454 5466 5634
Size: 588 138 159 282 147 108 12 168
Cuts at: 5634 5880
Size: 246
Fragments arranged by size:
630 588 510 478 342 290 285 282
276 246 235 201 168 168 159 147
147 146 138 114 108 105 69 36
12
RsrII CG'GwC_CG
Cuts at: 0 5516 5880
Size: 5516 364
SacI G_AGCT'C
Cuts at: 0 266 2246 2705 3200 3221 3254 3482 4534
Size: 266 1980 459 495 21 33 228 1052
Cuts at: 4534 5880
Size: 1346
Fragments arranged by size:
1980 1346 1052 495 459 266 228 33
21
SacII CC_GC'GG
Cuts at: 0 5075 5880
Size: 5075 805
SapI GCTCTTCn'nnn_
Cuts at: 0 1441 2494 3092 3410 4619 5020 5880
Size: 1441 1053 598 318 1209 401 860
Fragments arranged by size:
1441 1209 1053 860 598 401 318
SbfI CC_TGCA'GG
Cuts at: 0 2804 4688 4721 5880
Size: 2804 1884 33 1159
Fragments arranged by size:
2804 1884 1159 33
SexAI A'CCwGG_T
Cuts at: 0 1865 5880
Size: 1865 4015
SfcI C'TryA_G
Cuts at: 0 471 634 1426 1522 2367 2512 2620 2653
Size: 471 163 792 96 845 145 108 33
Cuts at: 2653 2800 2884 3709 3760 3769 3832 3928 3949
Size: 147 84 825 51 9 63 96 21
Cuts at: 3949 4252 4642 4684 4717 4927 5269 5380 5545
Size: 303 390 42 33 210 342 111 165
Cuts at: 5545 5686 5880
Size: 141 194
Fragments arranged by size:
845 825 792 471 390 342 303 210
194 165 163 147 145 141 111 108
96 96 84 63 51 42 33 33
21 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3265 4696 5662 5880
Size: 3265 1431 966 218
Fragments arranged by size:
3265 1431 966 218
SfoI GGC'GCC
Cuts at: 0 1332 5814 5880
Size: 1332 4482 66
Fragments arranged by size:
4482 1332 66
SmaI CCC'GGG
Cuts at: 0 933 1565 1899 4447 5880
Size: 933 632 334 2548 1433
Fragments arranged by size:
2548 1433 933 632 334
SmlI C'TyrA_G
Cuts at: 0 136 301 2092 2842 3061 3220 3307 3643
Size: 136 165 1791 750 219 159 87 336
Cuts at: 3643 3991 5368 5434 5623 5880
Size: 348 1377 66 189 257
Fragments arranged by size:
1791 1377 750 348 336 257 219 189
165 159 136 87 66
SphI G_CATG'C
Cuts at: 0 4953 5880
Size: 4953 927
SrfI GCCC'GGGC
Cuts at: 0 4447 5880
Size: 4447 1433
StuI AGG'CCT
Cuts at: 0 344 658 1246 2362 5419 5880
Size: 344 314 588 1116 3057 461
Fragments arranged by size:
3057 1116 588 461 344 314
StyI C'CwwG_G
Cuts at: 0 242 803 965 1247 1253 2189 2495 2546
Size: 242 561 162 282 6 936 306 51
Cuts at: 2546 2972 3408 3899 4427 4463 4535 5480 5880
Size: 426 436 491 528 36 72 945 400
Fragments arranged by size:
945 936 561 528 491 436 426 400
306 282 242 162 72 51 36 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 506 883 1162 1669 5533 5880
Size: 87 419 377 279 507 3864 347
Fragments arranged by size:
3864 507 419 377 347 279 87
TatI w'GTAC_w
Cuts at: 0 410 5411 5579 5880
Size: 410 5001 168 301
Fragments arranged by size:
5001 410 301 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 3782 3814 5880
Size: 1576 32 2174 32 2066
Fragments arranged by size:
2174 2066 1576 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3820 5880
Size: 3820 2060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 648 981 1245 1455 2196 3834 4059
Size: 168 480 333 264 210 741 1638 225
Cuts at: 4059 4542 4668 5880
Size: 483 126 1212
Fragments arranged by size:
1638 1212 741 483 480 333 264 225
210 168 126
XhoI C'TCGA_G
Cuts at: 0 2092 3643 5434 5880
Size: 2092 1551 1791 446
Fragments arranged by size:
2092 1791 1551 446
XmaI C'CCGG_G
Cuts at: 0 931 1563 1897 4445 5880
Size: 931 632 334 2548 1435
Fragments arranged by size:
2548 1435 931 632 334
XmnI GAAnn'nnTTC
Cuts at: 0 4018 5880
Size: 4018 1862
ZraI GAC'GTC
Cuts at: 0 2343 5880
Size: 2343 3537
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII
RsrII SacI SacII SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AleI AscI AseI AsiSI
AvrII BaeI BciVI BfrBI BmtI BsaWI BspEI BssHII
BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoRV
FseI HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PshAI PsiI
PsrI PvuI SalI SanDI ScaI SgfI SgrAI SnaBI
SpeI SspI SwaI XbaI