Restriction map for
pF1KSDB0017
(Linear) (Six-base) MAPSORT of: KIBB0017.seq Check: 5777 from: 1 to: 5220
KIBB0017.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 648 3213 5220
Size: 648 2565 2007
Fragments arranged by size:
2565 2007 648
AatII G_ACGT'C
Cuts at: 0 2848 5220
Size: 2848 2372
AccI GT'mk_AC
Cuts at: 0 2366 4745 5220
Size: 2366 2379 475
Fragments arranged by size:
2379 2366 475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1090 1341 1696 2137 2781 4570 5220
Size: 334 756 251 355 441 644 1789 650
Fragments arranged by size:
1789 756 650 644 441 355 334 251
AfeI AGC'GCT
Cuts at: 0 3586 3767 5220
Size: 3586 181 1453
Fragments arranged by size:
3586 1453 181
AflIII A'CryG_T
Cuts at: 0 824 1750 4592 5220
Size: 824 926 2842 628
Fragments arranged by size:
2842 926 824 628
AhdI GACnn_n'nnGTC
Cuts at: 0 1404 4734 5220
Size: 1404 3330 486
Fragments arranged by size:
3330 1404 486
AleI CACnn'nnGTG
Cuts at: 0 1753 2396 4891 5220
Size: 1753 643 2495 329
Fragments arranged by size:
2495 1753 643 329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1408 1569 1849 5220
Size: 1408 161 280 3371
Fragments arranged by size:
3371 1408 280 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 4597 4629 5220
Size: 1264 32 3301 32 591
Fragments arranged by size:
3301 1264 591 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 663 1332 1341 1533 3315 3558 3942 3978
Size: 663 669 9 192 1782 243 384 36
Cuts at: 3978 5220
Size: 1242
Fragments arranged by size:
1782 1242 669 663 384 243 192 36
9
ApaI G_GGCC'C
Cuts at: 0 2805 3240 5220
Size: 2805 435 1980
Fragments arranged by size:
2805 1980 435
ApaLI G'TGCA_C
Cuts at: 0 5101 5220
Size: 5101 119
ApoI r'AATT_y
Cuts at: 0 214 523 553 5220
Size: 214 309 30 4667
Fragments arranged by size:
4667 309 214 30
AseI AT'TA_AT
Cuts at: 0 2317 5220
Size: 2317 2903
AvaI C'yCGr_G
Cuts at: 0 1370 1489 2782 3120 3160 3239 3386 3964
Size: 1370 119 1293 338 40 79 147 578
Cuts at: 3964 3991 4095 4120 4172 5220
Size: 27 104 25 52 1048
Fragments arranged by size:
1370 1293 1048 578 338 147 119 104
79 52 40 27 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2830 2863 5220
Size: 2830 33 2357
Fragments arranged by size:
2830 2357 33
BanI G'GyrC_C
Cuts at: 0 793 1375 2016 2254 2696 3189 3286 3382
Size: 793 582 641 238 442 493 97 96
Cuts at: 3382 3630 4777 5220
Size: 248 1147 443
Fragments arranged by size:
1147 793 641 582 493 443 442 248
238 97 96
BanII G_rGCy'C
Cuts at: 0 363 755 918 1058 1139 2805 2933 3154
Size: 363 392 163 140 81 1666 128 221
Cuts at: 3154 3240 3740 4033 4475 5150 5220
Size: 86 500 293 442 675 70
Fragments arranged by size:
1666 675 500 442 392 363 293 221
163 140 128 86 81 70
BbeI G_GCGC'C
Cuts at: 0 1379 2020 2258 3193 3290 5220
Size: 1379 641 238 935 97 1930
Fragments arranged by size:
1930 1379 935 641 238 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 1687 1978 2961 3268 3412 4109 4644
Size: 407 1280 291 983 307 144 697 535
Cuts at: 4644 5220
Size: 576
Fragments arranged by size:
1280 983 697 576 535 407 307 291
144
BbvCI CC'TCA_GC
Cuts at: 0 3375 5220
Size: 3375 1845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3855 3889 5220
Size: 3855 34 1331
Fragments arranged by size:
3855 1331 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2831 5220
Size: 2831 2389
BglI GCCn_nnn'nGGC
Cuts at: 0 276 2515 3574 3646 4090 4721 5220
Size: 276 2239 1059 72 444 631 499
Fragments arranged by size:
2239 1059 631 499 444 276 72
BlpI GC'TnA_GC
Cuts at: 0 3514 3581 4027 5220
Size: 3514 67 446 1193
Fragments arranged by size:
3514 1193 446 67
Bme1580I G_kGCm'C
Cuts at: 0 2054 2805 3240 3431 5105 5220
Size: 2054 751 435 191 1674 115
Fragments arranged by size:
2054 1674 751 435 191 115
BmgBI CAC'GTC
Cuts at: 0 1401 2673 3297 4017 4593 4686 5220
Size: 1401 1272 624 720 576 93 534
Fragments arranged by size:
1401 1272 720 624 576 534 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 1521 3303 4151 4283 5220
Size: 70 1451 1782 848 132 937
Fragments arranged by size:
1782 1451 937 848 132 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1977 3168 3390 4515 5220
Size: 1977 1191 222 1125 705
Fragments arranged by size:
1977 1191 1125 705 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 439 1042 1099 2256 2260 2395 2551
Size: 304 135 603 57 1157 4 135 156
Cuts at: 2551 2947 3138 3223 3317 3523 3801 5123 5220
Size: 396 191 85 94 206 278 1322 97
Fragments arranged by size:
1322 1157 603 396 304 278 206 191
156 135 135 97 94 85 57 4
Bpu10I CC'TnA_GC
Cuts at: 0 1138 3375 3943 5220
Size: 1138 2237 568 1277
Fragments arranged by size:
2237 1277 1138 568
BsaI GGTCTCn'nnnn_
Cuts at: 0 654 1211 2820 3005 5220
Size: 654 557 1609 185 2215
Fragments arranged by size:
2215 1609 654 557 185
BsaAI yAC'GTr
Cuts at: 0 4809 5220
Size: 4809 411
BsaHI Gr'CG_yC
Cuts at: 0 668 1376 2017 2255 2845 3190 3287 4232
Size: 668 708 641 238 590 345 97 945
Cuts at: 4232 5220
Size: 988
Fragments arranged by size:
988 945 708 668 641 590 345 238
97
BsaWI w'CCGG_w
Cuts at: 0 173 620 2711 3464 4756 5220
Size: 173 447 2091 753 1292 464
Fragments arranged by size:
2091 1292 753 464 447 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1165 1195 1264 1294 1648 1678 1989 2019
Size: 1165 30 69 30 354 30 311 30
Cuts at: 2019 2524 2554 2799 2829 2956 2986 3118 3148
Size: 505 30 245 30 127 30 132 30
Cuts at: 3148 3667 3697 3817 3847 4171 4201 4738 4768
Size: 519 30 120 30 324 30 537 30
Cuts at: 4768 5220
Size: 452
Fragments arranged by size:
1165 537 519 505 452 354 324 311
245 132 127 120 69 30 30 30
30 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 893 1189 1456 1459 1982 3516 3650 3715
Size: 893 296 267 3 523 1534 134 65
Cuts at: 3715 3841 3862 3879 3948 4387 5181 5220
Size: 126 21 17 69 439 794 39
Fragments arranged by size:
1534 893 794 523 439 296 267 134
126 69 65 39 21 17 3
BseYI C'CCAG_C
Cuts at: 0 2040 3214 3446 5086 5220
Size: 2040 1174 232 1640 134
Fragments arranged by size:
2040 1640 1174 232 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1861 3332 3418 4411 4878 4933 5114
Size: 346 1515 1471 86 993 467 55 181
Cuts at: 5114 5220
Size: 106
Fragments arranged by size:
1515 1471 993 467 346 181 106 86
55
BsiEI CG_ry'CG
Cuts at: 0 254 280 3103 4387 5220
Size: 254 26 2823 1284 833
Fragments arranged by size:
2823 1284 833 254 26
BsiHKAI G_wGCw'C
Cuts at: 0 227 755 1058 1139 2165 2203 2933 3154
Size: 227 528 303 81 1026 38 730 221
Cuts at: 3154 3599 4475 5105 5220
Size: 445 876 630 115
Fragments arranged by size:
1026 876 730 630 528 445 303 227
221 115 81 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3891 5220
Size: 3891 1329
Bsp1286I G_dGCh'C
Cuts at: 0 227 363 755 918 1058 1139 2054 2165
Size: 227 136 392 163 140 81 915 111
Cuts at: 2165 2203 2805 2933 3154 3240 3431 3599 3740
Size: 38 602 128 221 86 191 168 141
Cuts at: 3740 4033 4475 5105 5150 5220
Size: 293 442 630 45 70
Fragments arranged by size:
915 630 602 442 392 293 227 221
191 168 163 141 140 136 128 111
86 81 70 45 38
BspEI T'CCGG_A
Cuts at: 0 173 620 2711 3464 5220
Size: 173 447 2091 753 1756
Fragments arranged by size:
2091 1756 753 447 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 648 1340 3213 3791 4928 5220
Size: 648 692 1873 578 1137 292
Fragments arranged by size:
1873 1137 692 648 578 292
BsrBI CCG'CTC
Cuts at: 0 276 1610 2244 3513 4151 4383 4985 5220
Size: 276 1334 634 1269 638 232 602 235
Fragments arranged by size:
1334 1269 638 634 602 276 235 232
BsrFI r'CCGG_y
Cuts at: 0 693 920 3192 3300 3633 3902 4022 4535
Size: 693 227 2272 108 333 269 120 513
Cuts at: 4535 5220
Size: 685
Fragments arranged by size:
2272 693 685 513 333 269 227 120
108
BsrGI T'GTAC_A
Cuts at: 0 1724 3614 5220
Size: 1724 1890 1606
Fragments arranged by size:
1890 1724 1606
BstAPI GCAn_nnn'nTGC
Cuts at: 0 300 3978 5220
Size: 300 3678 1242
Fragments arranged by size:
3678 1242 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4686 5220
Size: 4686 534
BstYI r'GATC_y
Cuts at: 0 258 3455 4406 5057 5220
Size: 258 3197 951 651 163
Fragments arranged by size:
3197 951 651 258 163
Bsu36I CC'TnA_GG
Cuts at: 0 4034 5220
Size: 4034 1186
BtgI C'CryG_G
Cuts at: 0 122 671 977 1166 1200 2294 2582 2888
Size: 122 549 306 189 34 1094 288 306
Cuts at: 2888 3231 3270 3340 3689 5220
Size: 343 39 70 349 1531
Fragments arranged by size:
1531 1094 549 349 343 306 306 288
189 122 70 39 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 567 4644 5220
Size: 567 4077 576
Fragments arranged by size:
4077 576 567
BtsI GCAGTG_nn'
Cuts at: 0 236 502 1873 2089 2190 2524 3327 4861
Size: 236 266 1371 216 101 334 803 1534
Cuts at: 4861 5220
Size: 359
Fragments arranged by size:
1534 1371 803 359 334 266 236 216
101
DraIII CAC_nnn'GTG
Cuts at: 0 231 300 5220
Size: 231 69 4920
Fragments arranged by size:
4920 231 69
DrdI GACnn_nn'nnGTC
Cuts at: 0 646 673 1507 5220
Size: 646 27 834 3713
Fragments arranged by size:
3713 834 646 27
EaeI y'GGCC_r
Cuts at: 0 2 277 1307 1520 1649 2291 2516 2549
Size: 2 275 1030 213 129 642 225 33
Cuts at: 2549 3194 3647 3900 4608 4895 5220
Size: 645 453 253 708 287 325
Fragments arranged by size:
1030 708 645 642 453 325 287 275
253 225 213 129 33 2
EagI C'GGCC_G
Cuts at: 0 277 5220
Size: 277 4943
EarI CTCTTCn'nnn_
Cuts at: 0 215 370 467 2071 2989 5220
Size: 215 155 97 1604 918 2231
Fragments arranged by size:
2231 1604 918 215 155 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3466 3675 5020 5038 5220
Size: 3466 209 1345 18 182
Fragments arranged by size:
3466 1345 209 182 18
EcoICRI GAG'CTC
Cuts at: 0 753 1056 1137 2931 3152 4473 5220
Size: 753 303 81 1794 221 1321 747
Fragments arranged by size:
1794 1321 753 747 303 221 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 334 439 1042 1090 1099 1341 1696
Size: 304 30 105 603 48 9 242 355
Cuts at: 1696 2137 2256 2260 2395 2551 2781 2947 3138
Size: 441 119 4 135 156 230 166 191
Cuts at: 3138 3223 3317 3523 3801 4570 5123 5220
Size: 85 94 206 278 769 553 97
Fragments arranged by size:
769 603 553 441 355 304 278 242
230 206 191 166 156 135 119 105
97 94 85 48 30 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 838 1546 1997 2377 3798 5220
Size: 838 708 451 380 1421 1422
Fragments arranged by size:
1422 1421 838 708 451 380
EcoO109I rG'GnC_Cy
Cuts at: 0 351 432 2002 2026 2382 2742 2801 3095
Size: 351 81 1570 24 356 360 59 294
Cuts at: 3095 3236 3996 4061 4126 4875 5220
Size: 141 760 65 65 749 345
Fragments arranged by size:
1570 760 749 360 356 351 345 294
141 81 65 65 59 24
EcoRI G'AATT_C
Cuts at: 0 523 5220
Size: 523 4697
EcoRV GAT'ATC
Cuts at: 0 5001 5220
Size: 5001 219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2811 5220
Size: 2811 2409
FseI GG_CCGG'CC
Cuts at: 0 3906 5220
Size: 3906 1314
FspI TGC'GCA
Cuts at: 0 1384 5220
Size: 1384 3836
FspAI rTGC'GCAy
Cuts at: 0 1384 5220
Size: 1384 3836
HaeII r_GCGC'y
Cuts at: 0 815 1342 1379 2020 2258 2467 3193 3290
Size: 815 527 37 641 238 209 726 97
Cuts at: 3290 3588 3769 4716 5184 5220
Size: 298 181 947 468 36
Fragments arranged by size:
947 815 726 641 527 468 298 238
209 181 97 37 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 591 623 985 1017 1390 1422
Size: 63 32 496 32 362 32 373 32
Cuts at: 1422 2524 2556 2799 2831 2869 2901 3118 3150
Size: 1102 32 243 32 38 32 217 32
Cuts at: 3150 3817 3849 4357 4389 4720 4752 5220
Size: 667 32 508 32 331 32 468
Fragments arranged by size:
1102 667 508 496 468 373 362 331
243 217 63 38 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1163 5220
Size: 1163 4057
HindIII A'AGCT_T
Cuts at: 0 3061 4771 5220
Size: 3061 1710 449
Fragments arranged by size:
3061 1710 449
KasI G'GCGC_C
Cuts at: 0 1375 2016 2254 3189 3286 5220
Size: 1375 641 238 935 97 1934
Fragments arranged by size:
1934 1375 935 641 238 97
MluI A'CGCG_T
Cuts at: 0 1750 5220
Size: 1750 3470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 2999 3698 3728 4214 4428 5012 5105
Size: 419 2580 699 30 486 214 584 93
Cuts at: 5105 5220
Size: 115
Fragments arranged by size:
2580 699 584 486 419 214 115 93
30
MscI TGG'CCA
Cuts at: 0 4 1522 2551 4897 5220
Size: 4 1518 1029 2346 323
Fragments arranged by size:
2346 1518 1029 323 4
MslI CAynn'nnrTG
Cuts at: 0 175 1390 1753 2396 2680 4669 4891 5220
Size: 175 1215 363 643 284 1989 222 329
Fragments arranged by size:
1989 1215 643 363 329 284 222 175
MspA1I CmG'CkG
Cuts at: 0 124 282 711 1979 2196 2396 2584 3379
Size: 124 158 429 1268 217 200 188 795
Cuts at: 3379 3488 4700 4824 5055 5175 5220
Size: 109 1212 124 231 120 45
Fragments arranged by size:
1268 1212 795 429 231 217 200 188
158 124 124 120 109 45
NaeI GCC'GGC
Cuts at: 0 695 3194 3302 3635 3904 4024 4537 5220
Size: 695 2499 108 333 269 120 513 683
Fragments arranged by size:
2499 695 683 513 333 269 120 108
NarI GG'CG_CC
Cuts at: 0 1376 2017 2255 3190 3287 5220
Size: 1376 641 238 935 97 1933
Fragments arranged by size:
1933 1376 935 641 238 97
NcoI C'CATG_G
Cuts at: 0 977 3270 5220
Size: 977 2293 1950
Fragments arranged by size:
2293 1950 977
NgoMIV G'CCGG_C
Cuts at: 0 693 3192 3300 3633 3902 4022 4535 5220
Size: 693 2499 108 333 269 120 513 685
Fragments arranged by size:
2499 693 685 513 333 269 120 108
NotI GC'GGCC_GC
Cuts at: 0 277 5220
Size: 277 4943
NruI TCG'CGA
Cuts at: 0 1819 5220
Size: 1819 3401
NspI r_CATG'y
Cuts at: 0 828 986 3172 3546 5220
Size: 828 158 2186 374 1674
Fragments arranged by size:
2186 1674 828 374 158
PasI CC'CwG_GG
Cuts at: 0 3441 3742 4000 5220
Size: 3441 301 258 1220
Fragments arranged by size:
3441 1220 301 258
PciI A'CATG_T
Cuts at: 0 824 5220
Size: 824 4396
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 978 1065 1122 1533 3044 3315 5220
Size: 837 141 87 57 411 1511 271 1905
Fragments arranged by size:
1905 1511 837 411 271 141 87 57
PfoI T'CCnGG_A
Cuts at: 0 3333 5220
Size: 3333 1887
PmlI CAC'GTG
Cuts at: 0 4809 5220
Size: 4809 411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 5220
Size: 1264 32 3924
Fragments arranged by size:
3924 1264 32
PpuMI rG'GwC_Cy
Cuts at: 0 351 3095 3996 4061 4126 5220
Size: 351 2744 901 65 65 1094
Fragments arranged by size:
2744 1094 901 351 65 65
PshAI GACnn'nnGTC
Cuts at: 0 1147 1687 5220
Size: 1147 540 3533
Fragments arranged by size:
3533 1147 540
PspOMI G'GGCC_C
Cuts at: 0 2801 3236 5220
Size: 2801 435 1984
Fragments arranged by size:
2801 1984 435
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4501 4533 5220
Size: 4501 32 687
Fragments arranged by size:
4501 687 32
PstI C_TGCA'G
Cuts at: 0 93 497 659 1337 1838 2025 2084 2434
Size: 93 404 162 678 501 187 59 350
Cuts at: 2434 2877 3134 3554 3974 3983 4307 4856 5220
Size: 443 257 420 420 9 324 549 364
Fragments arranged by size:
678 549 501 443 420 420 404 364
350 324 257 187 162 93 59 9
PvuII CAG'CTG
Cuts at: 0 2196 3379 3488 5220
Size: 2196 1183 109 1732
Fragments arranged by size:
2196 1732 1183 109
RsrII CG'GwC_CG
Cuts at: 0 623 5220
Size: 623 4597
SacI G_AGCT'C
Cuts at: 0 755 1058 1139 2933 3154 4475 5220
Size: 755 303 81 1794 221 1321 745
Fragments arranged by size:
1794 1321 755 745 303 221 81
SacII CC_GC'GG
Cuts at: 0 125 2585 5220
Size: 125 2460 2635
Fragments arranged by size:
2635 2460 125
SanDI GG'GwC_CC
Cuts at: 0 3095 3996 4061 4126 5220
Size: 3095 901 65 65 1094
Fragments arranged by size:
3095 1094 901 65 65
SapI GCTCTTCn'nnn_
Cuts at: 0 215 467 2071 5220
Size: 215 252 1604 3149
Fragments arranged by size:
3149 1604 252 215
SbfI CC_TGCA'GG
Cuts at: 0 659 1337 2025 3554 5220
Size: 659 678 688 1529 1666
Fragments arranged by size:
1666 1529 688 678 659
ScaI AGT'ACT
Cuts at: 0 2811 5220
Size: 2811 2409
SexAI A'CCwGG_T
Cuts at: 0 1505 4841 5220
Size: 1505 3336 379
Fragments arranged by size:
3336 1505 379
SfcI C'TryA_G
Cuts at: 0 89 493 655 1284 1333 1834 2021 2080
Size: 89 404 162 629 49 501 187 59
Cuts at: 2080 2430 2568 2873 3130 3550 3795 3970 3979
Size: 350 138 305 257 420 245 175 9
Cuts at: 3979 4303 4503 4852 5220
Size: 324 200 349 368
Fragments arranged by size:
629 501 420 404 368 350 349 324
305 257 245 200 187 175 162 138
89 59 49 9
SfoI GGC'GCC
Cuts at: 0 1377 2018 2256 3191 3288 5220
Size: 1377 641 238 935 97 1932
Fragments arranged by size:
1932 1377 935 641 238 97
SmaI CCC'GGG
Cuts at: 0 3162 3241 3388 5220
Size: 3162 79 147 1832
Fragments arranged by size:
3162 1832 147 79
SpeI A'CTAG_T
Cuts at: 0 2812 5220
Size: 2812 2408
StuI AGG'CCT
Cuts at: 0 1254 1840 3113 3373 4586 5220
Size: 1254 586 1273 260 1213 634
Fragments arranged by size:
1273 1254 1213 634 586 260
StyI C'CwwG_G
Cuts at: 0 789 977 1121 1310 1667 3182 3270 4679
Size: 789 188 144 189 357 1515 88 1409
Cuts at: 4679 5220
Size: 541
Fragments arranged by size:
1515 1409 789 541 357 189 188 144
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 811 1540 4438 5220
Size: 811 729 2898 782
Fragments arranged by size:
2898 811 782 729
TatI w'GTAC_w
Cuts at: 0 578 1724 2648 2809 3614 5220
Size: 578 1146 924 161 805 1606
Fragments arranged by size:
1606 1146 924 805 578 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1989 2021 2182 2214 3375 3407
Size: 1165 32 792 32 161 32 1161 32
Cuts at: 3407 4819 4851 5095 5127 5220
Size: 1412 32 244 32 93
Fragments arranged by size:
1412 1165 1161 792 244 161 93 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 487 627 3099 5220
Size: 487 140 2472 2121
Fragments arranged by size:
2472 2121 487 140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1530 2433 2511 2862 3045 4683 5094 5220
Size: 1530 903 78 351 183 1638 411 126
Fragments arranged by size:
1638 1530 903 411 351 183 126 78
XmaI C'CCGG_G
Cuts at: 0 3160 3239 3386 5220
Size: 3160 79 147 1834
Fragments arranged by size:
3160 1834 147 79
XmnI GAAnn'nnTTC
Cuts at: 0 404 527 1195 5220
Size: 404 123 668 4025
Fragments arranged by size:
4025 668 404 123
ZraI GAC'GTC
Cuts at: 0 2846 5220
Size: 2846 2374
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfcI SfoI SmaI
SpeI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AscI AsiSI AvrII BamHI
BclI BfrBI BglII BmtI BpuEI BsaBI BsiWI BsmI
BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I ClaI
CspCI DraI HpaI KpnI MfeI NdeI NheI NsiI
PacI PmeI PsiI PspXI PvuI SalI SfiI SgfI
SgrAI SmlI SnaBI SphI SrfI SspI SwaI XbaI
XhoI