Restriction map for
pF1KB0020
(Linear) (Six-base) MAPSORT of: KIBB0020.seq Check: 2023 from: 1 to: 5349
KIBB0020.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4330 5349
Size: 4330 1019
AatII G_ACGT'C
Cuts at: 0 4723 5349
Size: 4723 626
AccI GT'mk_AC
Cuts at: 0 1004 5349
Size: 1004 4345
Acc65I G'GTAC_C
Cuts at: 0 3607 5349
Size: 3607 1742
AclI AA'CG_TT
Cuts at: 0 4327 5349
Size: 4327 1022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 437 748 953 1196 1352 1796 2732
Size: 356 81 311 205 243 156 444 936
Cuts at: 2732 2801 3008 3181 3263 3277 3920 4121 4543
Size: 69 207 173 82 14 643 201 422
Cuts at: 4543 4823 5349
Size: 280 526
Fragments arranged by size:
936 643 526 444 422 356 311 280
243 207 205 201 173 156 82 81
69 14
AfeI AGC'GCT
Cuts at: 0 1920 5349
Size: 1920 3429
AflII C'TTAA_G
Cuts at: 0 4485 5349
Size: 4485 864
AflIII A'CryG_T
Cuts at: 0 1405 1729 5349
Size: 1405 324 3620
Fragments arranged by size:
3620 1405 324
AgeI A'CCGG_T
Cuts at: 0 3175 5349
Size: 3175 2174
AleI CACnn'nnGTG
Cuts at: 0 2635 3061 4524 5349
Size: 2635 426 1463 825
Fragments arranged by size:
2635 1463 825 426
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3455 3487 5349
Size: 3455 32 1862
Fragments arranged by size:
3455 1862 32
AlwNI CAG_nnn'CTG
Cuts at: 0 634 1321 1951 2386 2632 2683 2941 3643
Size: 634 687 630 435 246 51 258 702
Cuts at: 3643 4270 4968 5349
Size: 627 698 381
Fragments arranged by size:
702 698 687 634 630 627 435 381
258 246 51
ApaI G_GGCC'C
Cuts at: 0 4430 5349
Size: 4430 919
ApaLI G'TGCA_C
Cuts at: 0 4343 5254 5349
Size: 4343 911 95
Fragments arranged by size:
4343 911 95
ApoI r'AATT_y
Cuts at: 0 544 752 892 4226 5349
Size: 544 208 140 3334 1123
Fragments arranged by size:
3334 1123 544 208 140
AvaI C'yCGr_G
Cuts at: 0 46 73 1717 2614 2879 5349
Size: 46 27 1644 897 265 2470
Fragments arranged by size:
2470 1644 897 265 46 27
AvrII C'CTAG_G
Cuts at: 0 4197 5349
Size: 4197 1152
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2012 2045 5349
Size: 2012 33 3304
Fragments arranged by size:
3304 2012 33
BamHI G'GATC_C
Cuts at: 0 2180 5349
Size: 2180 3169
BanI G'GyrC_C
Cuts at: 0 4 108 2956 3172 3607 5349
Size: 4 104 2848 216 435 1742
Fragments arranged by size:
2848 1742 435 216 104 4
BanII G_rGCy'C
Cuts at: 0 16 58 74 80 221 261 279 1103
Size: 16 42 16 6 141 40 18 824
Cuts at: 1103 1696 1718 2651 4243 4430 5349
Size: 593 22 933 1592 187 919
Fragments arranged by size:
1592 933 919 824 593 187 141 42
40 22 18 16 16 6
BbeI G_GCGC'C
Cuts at: 0 8 112 5349
Size: 8 104 5237
Fragments arranged by size:
5237 104 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1022 1922 2348 2492 3725 4391 4415 5349
Size: 1022 900 426 144 1233 666 24 934
Fragments arranged by size:
1233 1022 934 900 666 426 144 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1678 1712 2962 2996 5349
Size: 1678 34 1250 34 2353
Fragments arranged by size:
2353 1678 1250 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2013 3774 5349
Size: 2013 1761 1575
Fragments arranged by size:
2013 1761 1575
BclI T'GATC_A
Cuts at: 0 4959 5349
Size: 4959 390
BglI GCCn_nnn'nGGC
Cuts at: 0 106 4425 5349
Size: 106 4319 924
Fragments arranged by size:
4319 924 106
BlpI GC'TnA_GC
Cuts at: 0 68 206 220 1746 2650 5349
Size: 68 138 14 1526 904 2699
Fragments arranged by size:
2699 1526 904 138 68 14
Bme1580I G_kGCm'C
Cuts at: 0 1095 3625 4142 4347 4421 4430 5258 5349
Size: 1095 2530 517 205 74 9 828 91
Fragments arranged by size:
2530 1095 828 517 205 91 74 9
BmgBI CAC'GTC
Cuts at: 0 1260 5349
Size: 1260 4089
BmrI ACTGGGnnnn_n'
Cuts at: 0 3015 3606 5349
Size: 3015 591 1743
Fragments arranged by size:
3015 1743 591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 1053 1155 1459 3131 4386 5349
Size: 72 981 102 304 1672 1255 963
Fragments arranged by size:
1672 1255 981 963 304 102 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 686 2363 3112 3647 4108 5349
Size: 211 475 1677 749 535 461 1241
Fragments arranged by size:
1677 1241 749 535 475 461 211
Bpu10I CC'TnA_GC
Cuts at: 0 3293 3429 5349
Size: 3293 136 1920
Fragments arranged by size:
3293 1920 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 3101 3356 3776 3871 4093 4648 5349
Size: 887 2214 255 420 95 222 555 701
Fragments arranged by size:
2214 887 701 555 420 255 222 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 715 2120 2418 2732 2986 4624 5349
Size: 715 1405 298 314 254 1638 725
Fragments arranged by size:
1638 1405 725 715 314 298 254
BsaAI yAC'GTr
Cuts at: 0 1730 4690 4746 5349
Size: 1730 2960 56 603
Fragments arranged by size:
2960 1730 603 56
BsaHI Gr'CG_yC
Cuts at: 0 5 109 1852 4720 5349
Size: 5 104 1743 2868 629
Fragments arranged by size:
2868 1743 629 104 5
BsaWI w'CCGG_w
Cuts at: 0 1414 1662 3060 3175 5349
Size: 1414 248 1398 115 2174
Fragments arranged by size:
2174 1414 1398 248 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1714 1744 4843 4873 5349
Size: 1714 30 3099 30 476
Fragments arranged by size:
3099 1714 476 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 1159 1288 1625 1628 1631 1690 1726
Size: 433 726 129 337 3 3 59 36
Cuts at: 1726 1735 2138 2815 3064 3359 3601 3974 4111
Size: 9 403 677 249 295 242 373 137
Cuts at: 4111 4178 4222 4291 4606 4864 5311 5349
Size: 67 44 69 315 258 447 38
Fragments arranged by size:
726 677 447 433 403 373 337 315
295 258 249 242 137 129 69 67
59 44 38 36 9 3 3
BseYI C'CCAG_C
Cuts at: 0 79 652 762 2353 3161 3433 5349
Size: 79 573 110 1591 808 272 1916
Fragments arranged by size:
1916 1591 808 573 272 110 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1552 1777 3085 3226 3629 4799 5349
Size: 1552 225 1308 141 403 1170 550
Fragments arranged by size:
1552 1308 1170 550 403 225 141
BsiEI CG_ry'CG
Cuts at: 0 101 2534 2894 5349
Size: 101 2433 360 2455
Fragments arranged by size:
2455 2433 360 101
BsiHKAI G_wGCw'C
Cuts at: 0 16 58 221 1640 1696 1945 2651 4079
Size: 16 42 163 1419 56 249 706 1428
Cuts at: 4079 4243 4347 5258 5349
Size: 164 104 911 91
Fragments arranged by size:
1428 1419 911 706 249 164 163 104
91 56 42 16
BsmI GAATG_Cn'
Cuts at: 0 796 5349
Size: 796 4553
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1613 2158 3259 5349
Size: 1613 545 1101 2090
Fragments arranged by size:
2090 1613 1101 545
Bsp1286I G_dGCh'C
Cuts at: 0 16 58 74 80 221 261 279 1095
Size: 16 42 16 6 141 40 18 816
Cuts at: 1095 1103 1640 1696 1718 1945 2651 3625 4079
Size: 8 537 56 22 227 706 974 454
Cuts at: 4079 4142 4243 4347 4421 4430 5258 5349
Size: 63 101 104 74 9 828 91
Fragments arranged by size:
974 828 816 706 537 454 227 141
104 101 91 74 63 56 42 40
22 18 16 16 9 8 6
BspEI T'CCGG_A
Cuts at: 0 1414 1662 5349
Size: 1414 248 3687
Fragments arranged by size:
3687 1414 248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1194 1383 4330 4542 5349
Size: 1194 189 2947 212 807
Fragments arranged by size:
2947 1194 807 212 189
BsrBI CCG'CTC
Cuts at: 0 4396 5349
Size: 4396 953
BsrDI GCAATG_nn'
Cuts at: 0 2123 5349
Size: 2123 3226
BsrFI r'CCGG_y
Cuts at: 0 63 913 1080 3175 5349
Size: 63 850 167 2095 2174
Fragments arranged by size:
2174 2095 850 167 63
BssSI C'ACGA_G
Cuts at: 0 1096 5349
Size: 1096 4253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1033 1090 2941 2983 3643 5349
Size: 1033 57 1851 42 660 1706
Fragments arranged by size:
1851 1706 1033 660 57 42
BstBI TT'CG_AA
Cuts at: 0 3842 5349
Size: 3842 1507
BstEII G'GTnAC_C
Cuts at: 0 235 5349
Size: 235 5114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1386 3003 3169 4771 5349
Size: 1386 1617 166 1602 578
Fragments arranged by size:
1617 1602 1386 578 166
BstYI r'GATC_y
Cuts at: 0 2180 5349
Size: 2180 3169
Bsu36I CC'TnA_GG
Cuts at: 0 1361 1709 4805 5160 5349
Size: 1361 348 3096 355 189
Fragments arranged by size:
3096 1361 355 348 189
BtgI C'CryG_G
Cuts at: 0 122 2534 2843 3995 5349
Size: 122 2412 309 1152 1354
Fragments arranged by size:
2412 1354 1152 309 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 5349
Size: 120 5229
BtsI GCAGTG_nn'
Cuts at: 0 788 3140 4874 5349
Size: 788 2352 1734 475
Fragments arranged by size:
2352 1734 788 475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4850 4885 5349
Size: 4850 35 464
Fragments arranged by size:
4850 464 35
DraI TTT'AAA
Cuts at: 0 543 2286 5349
Size: 543 1743 3063
Fragments arranged by size:
3063 1743 543
DrdI GACnn_nn'nnGTC
Cuts at: 0 2524 5349
Size: 2524 2825
EaeI y'GGCC_r
Cuts at: 0 98 246 774 1247 2531 5349
Size: 98 148 528 473 1284 2818
Fragments arranged by size:
2818 1284 528 473 148 98
EagI C'GGCC_G
Cuts at: 0 98 2531 5349
Size: 98 2433 2818
Fragments arranged by size:
2818 2433 98
EarI CTCTTCn'nnn_
Cuts at: 0 464 713 803 1172 1232 1280 1979 2094
Size: 464 249 90 369 60 48 699 115
Cuts at: 2094 2324 2984 3092 3227 4058 4097 4355 4967
Size: 230 660 108 135 831 39 258 612
Cuts at: 4967 4991 5349
Size: 24 358
Fragments arranged by size:
831 699 660 612 464 369 358 258
249 230 135 115 108 90 60 48
39 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2920 3126 5349
Size: 2920 206 2223
Fragments arranged by size:
2920 2223 206
EcoICRI GAG'CTC
Cuts at: 0 14 56 219 1694 2649 4241 5349
Size: 14 42 163 1475 955 1592 1108
Fragments arranged by size:
1592 1475 1108 955 163 42 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 356 437 686 748 953 1196 1352
Size: 211 145 81 249 62 205 243 156
Cuts at: 1352 1796 2363 2732 2801 3008 3112 3181 3263
Size: 444 567 369 69 207 104 69 82
Cuts at: 3263 3277 3647 3920 4108 4121 4543 4823 5349
Size: 14 370 273 188 13 422 280 526
Fragments arranged by size:
567 526 444 422 370 369 280 273
249 243 211 207 205 188 156 145
104 82 81 69 69 62 14 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 229 2252 5349
Size: 229 2023 3097
Fragments arranged by size:
3097 2023 229
EcoO109I rG'GnC_Cy
Cuts at: 0 648 1168 1422 2084 3123 4426 4800 5349
Size: 648 520 254 662 1039 1303 374 549
Fragments arranged by size:
1303 1039 662 648 549 520 374 254
EcoRV GAT'ATC
Cuts at: 0 2741 5349
Size: 2741 2608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2747 3211 3925 4087 4500 5349
Size: 2747 464 714 162 413 849
Fragments arranged by size:
2747 849 714 464 413 162
HaeII r_GCGC'y
Cuts at: 0 8 112 1922 5349
Size: 8 104 1810 3427
Fragments arranged by size:
3427 1810 104 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1612 1644 1716 1748 1802 1834 2745 2777
Size: 1612 32 72 32 54 32 911 32
Cuts at: 2777 3586 3618 4725 4757 4843 4875 5305 5337
Size: 809 32 1107 32 86 32 430 32
Cuts at: 5337 5349
Size: 12
Fragments arranged by size:
1612 1107 911 809 430 86 72 54
32 32 32 32 32 32 32 32
12
HincII GTy'rAC
Cuts at: 0 2795 3026 4677 4718 4862 5349
Size: 2795 231 1651 41 144 487
Fragments arranged by size:
2795 1651 487 231 144 41
HindIII A'AGCT_T
Cuts at: 0 965 1552 1579 2039 3354 4069 5349
Size: 965 587 27 460 1315 715 1280
Fragments arranged by size:
1315 1280 965 715 587 460 27
HpaI GTT'AAC
Cuts at: 0 2795 3026 5349
Size: 2795 231 2323
Fragments arranged by size:
2795 2323 231
KasI G'GCGC_C
Cuts at: 0 4 108 5349
Size: 4 104 5241
Fragments arranged by size:
5241 104 4
KpnI G_GTAC'C
Cuts at: 0 3611 5349
Size: 3611 1738
MfeI C'AATT_G
Cuts at: 0 4586 5349
Size: 4586 763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 874 4310 4517 5349
Size: 300 574 3436 207 832
Fragments arranged by size:
3436 832 574 300 207
MscI TGG'CCA
Cuts at: 0 248 776 5349
Size: 248 528 4573
Fragments arranged by size:
4573 528 248
MslI CAynn'nnrTG
Cuts at: 0 2635 3001 3061 4058 4524 4711 5349
Size: 2635 366 60 997 466 187 638
Fragments arranged by size:
2635 997 638 466 366 187 60
MspA1I CmG'CkG
Cuts at: 0 83 124 183 385 1114 1330 2683 2932
Size: 83 41 59 202 729 216 1353 249
Cuts at: 2932 3165 4087 4267 4968 5349
Size: 233 922 180 701 381
Fragments arranged by size:
1353 922 729 701 381 249 233 216
202 180 83 59 41
NaeI GCC'GGC
Cuts at: 0 65 915 1082 5349
Size: 65 850 167 4267
Fragments arranged by size:
4267 850 167 65
NarI GG'CG_CC
Cuts at: 0 5 109 5349
Size: 5 104 5240
Fragments arranged by size:
5240 104 5
NdeI CA'TA_TG
Cuts at: 0 2669 5349
Size: 2669 2680
NgoMIV G'CCGG_C
Cuts at: 0 63 913 1080 5349
Size: 63 850 167 4269
Fragments arranged by size:
4269 850 167 63
PasI CC'CwG_GG
Cuts at: 0 1826 5349
Size: 1826 3523
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 3135 5349
Size: 783 2352 2214
Fragments arranged by size:
2352 2214 783
PfoI T'CCnGG_A
Cuts at: 0 227 2433 3686 5349
Size: 227 2206 1253 1663
Fragments arranged by size:
2206 1663 1253 227
PmlI CAC'GTG
Cuts at: 0 1730 4746 5349
Size: 1730 3016 603
Fragments arranged by size:
3016 1730 603
PpuMI rG'GwC_Cy
Cuts at: 0 648 1168 1422 2084 3123 4800 5349
Size: 648 520 254 662 1039 1677 549
Fragments arranged by size:
1677 1039 662 648 549 520 254
PshAI GACnn'nnGTC
Cuts at: 0 1445 5349
Size: 1445 3904
PspOMI G'GGCC_C
Cuts at: 0 4426 5349
Size: 4426 923
PstI C_TGCA'G
Cuts at: 0 843 2937 2946 3968 4275 4341 5349
Size: 843 2094 9 1022 307 66 1008
Fragments arranged by size:
2094 1022 1008 843 307 66 9
PvuII CAG'CTG
Cuts at: 0 183 1114 1330 2683 2932 3165 4087 4968
Size: 183 931 216 1353 249 233 922 881
Cuts at: 4968 5349
Size: 381
Fragments arranged by size:
1353 931 922 881 381 249 233 216
183
SacI G_AGCT'C
Cuts at: 0 16 58 221 1696 2651 4243 5349
Size: 16 42 163 1475 955 1592 1106
Fragments arranged by size:
1592 1475 1106 955 163 42 16
SacII CC_GC'GG
Cuts at: 0 125 5349
Size: 125 5224
SapI GCTCTTCn'nnn_
Cuts at: 0 464 1280 1979 3092 3227 5349
Size: 464 816 699 1113 135 2122
Fragments arranged by size:
2122 1113 816 699 464 135
SbfI CC_TGCA'GG
Cuts at: 0 4341 5349
Size: 4341 1008
SfcI C'TryA_G
Cuts at: 0 166 704 839 2561 2933 2942 3497 3860
Size: 166 538 135 1722 372 9 555 363
Cuts at: 3860 3964 4271 4337 5349
Size: 104 307 66 1012
Fragments arranged by size:
1722 1012 555 538 372 363 307 166
135 104 66 9
SfoI GGC'GCC
Cuts at: 0 6 110 5349
Size: 6 104 5239
Fragments arranged by size:
5239 104 6
SgrAI Cr'CCGG_yG
Cuts at: 0 3175 5349
Size: 3175 2174
SmaI CCC'GGG
Cuts at: 0 2881 5349
Size: 2881 2468
SmlI C'TyrA_G
Cuts at: 0 902 3116 3371 3791 3850 4072 4485 4627
Size: 902 2214 255 420 59 222 413 142
Cuts at: 4627 5349
Size: 722
Fragments arranged by size:
2214 902 722 420 413 255 222 142
59
SnaBI TAC'GTA
Cuts at: 0 4690 5349
Size: 4690 659
SpeI A'CTAG_T
Cuts at: 0 853 5076 5349
Size: 853 4223 273
Fragments arranged by size:
4223 853 273
StuI AGG'CCT
Cuts at: 0 3660 4810 5349
Size: 3660 1150 539
Fragments arranged by size:
3660 1150 539
StyI C'CwwG_G
Cuts at: 0 657 1379 2642 4197 5349
Size: 657 722 1263 1555 1152
Fragments arranged by size:
1555 1263 1152 722 657
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1085 2944 5349
Size: 1085 1859 2405
Fragments arranged by size:
2405 1859 1085
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1632 1664 1714 1746 5349
Size: 1632 32 50 32 3603
Fragments arranged by size:
3603 1632 50 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 583 1909 2890 5349
Size: 583 1326 981 2459
Fragments arranged by size:
2459 1326 981 583
XbaI T'CTAG_A
Cuts at: 0 3726 5349
Size: 3726 1623
XmaI C'CCGG_G
Cuts at: 0 2879 5349
Size: 2879 2470
XmnI GAAnn'nnTTC
Cuts at: 0 443 5349
Size: 443 4906
ZraI GAC'GTC
Cuts at: 0 4721 5349
Size: 4721 628
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaI ApaLI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SapI SbfI SfcI SfoI SgrAI SmaI SmlI SnaBI
SpeI StuI StyI TaqII TstI Tth111I XbaI XmaI
XmnI ZraI
Enzymes that do not cut:
AhdI AlfI AscI AseI AsiSI BbvCI BfrBI BglII
BmtI BsaBI BsiWI BspHI BsrGI BssHII BstZ17I ClaI
DraIII EcoRI FseI FspI FspAI MluI NcoI NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI
SexAI SfiI SgfI SphI SrfI SspI SwaI TatI
XcmI XhoI