Restriction map for
pF1KSDB0022
(Linear) (Six-base) MAPSORT of: KIBB0022.seq Check: 333 from: 1 to: 1560
KIBB0022.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 907 1560
Size: 907 653
AgeI A'CCGG_T
Cuts at: 0 1323 1560
Size: 1323 237
AleI CACnn'nnGTG
Cuts at: 0 928 1271 1560
Size: 928 343 289
Fragments arranged by size:
928 343 289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1276 1560
Size: 1276 284
AlwNI CAG_nnn'CTG
Cuts at: 0 1507 1560
Size: 1507 53
ApaI G_GGCC'C
Cuts at: 0 1475 1560
Size: 1475 85
AvaI C'yCGr_G
Cuts at: 0 295 683 1248 1560
Size: 295 388 565 312
Fragments arranged by size:
565 388 312 295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1538 1560
Size: 1538 22
BamHI G'GATC_C
Cuts at: 0 1426 1560
Size: 1426 134
BanI G'GyrC_C
Cuts at: 0 435 1560
Size: 435 1125
BanII G_rGCy'C
Cuts at: 0 302 331 371 380 457 789 1031 1475
Size: 302 29 40 9 77 332 242 444
Cuts at: 1475 1522 1560
Size: 47 38
Fragments arranged by size:
444 332 302 242 77 47 40 38
29 9
BbvCI CC'TCA_GC
Cuts at: 0 1181 1202 1514 1560
Size: 1181 21 312 46
Fragments arranged by size:
1181 312 46 21
BfrBI ATG'CAT
Cuts at: 0 846 1560
Size: 846 714
BglI GCCn_nnn'nGGC
Cuts at: 0 591 1560
Size: 591 969
BglII A'GATC_T
Cuts at: 0 870 1560
Size: 870 690
BlpI GC'TnA_GC
Cuts at: 0 103 1521 1560
Size: 103 1418 39
Fragments arranged by size:
1418 103 39
Bme1580I G_kGCm'C
Cuts at: 0 440 1475 1560
Size: 440 1035 85
Fragments arranged by size:
1035 440 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 897 922 1489 1560
Size: 897 25 567 71
Fragments arranged by size:
897 567 71 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 1560
Size: 1050 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1349 1560
Size: 358 991 211
Fragments arranged by size:
991 358 211
Bpu10I CC'TnA_GC
Cuts at: 0 1181 1202 1391 1514 1560
Size: 1181 21 189 123 46
Fragments arranged by size:
1181 189 123 46 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1560
Size: 184 1376
BsaI GGTCTCn'nnnn_
Cuts at: 0 250 903 1411 1436 1560
Size: 250 653 508 25 124
Fragments arranged by size:
653 508 250 124 25
BsaAI yAC'GTr
Cuts at: 0 1269 1560
Size: 1269 291
BsaBI GATnn'nnATC
Cuts at: 0 19 1560
Size: 19 1541
BsaWI w'CCGG_w
Cuts at: 0 1323 1560
Size: 1323 237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 1560
Size: 331 30 1199
Fragments arranged by size:
1199 331 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 691 1023 1560
Size: 379 312 332 537
Fragments arranged by size:
537 379 332 312
BseYI C'CCAG_C
Cuts at: 0 475 542 587 615 652 776 790 884
Size: 475 67 45 28 37 124 14 94
Cuts at: 884 1109 1377 1416 1494 1560
Size: 225 268 39 78 66
Fragments arranged by size:
475 268 225 124 94 78 67 66
45 39 37 28 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 1560
Size: 1043 517
BsiHKAI G_wGCw'C
Cuts at: 0 371 457 684 1031 1560
Size: 371 86 227 347 529
Fragments arranged by size:
529 371 347 227 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1429 1560
Size: 1429 131
Bsp1286I G_dGCh'C
Cuts at: 0 302 331 371 380 440 457 684 789
Size: 302 29 40 9 60 17 227 105
Cuts at: 789 1031 1475 1522 1560
Size: 242 444 47 38
Fragments arranged by size:
444 302 242 227 105 60 47 40
38 29 17 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 815 1560
Size: 815 745
BsrBI CCG'CTC
Cuts at: 0 42 1560
Size: 42 1518
BsrDI GCAATG_nn'
Cuts at: 0 1083 1560
Size: 1083 477
BsrFI r'CCGG_y
Cuts at: 0 1323 1560
Size: 1323 237
BssSI C'ACGA_G
Cuts at: 0 226 1560
Size: 226 1334
BstEII G'GTnAC_C
Cuts at: 0 489 622 1560
Size: 489 133 938
Fragments arranged by size:
938 489 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 892 930 1560
Size: 892 38 630
Fragments arranged by size:
892 630 38
BstYI r'GATC_y
Cuts at: 0 20 870 1426 1560
Size: 20 850 556 134
Fragments arranged by size:
850 556 134 20
Bsu36I CC'TnA_GG
Cuts at: 0 262 1508 1560
Size: 262 1246 52
Fragments arranged by size:
1246 262 52
BtgI C'CryG_G
Cuts at: 0 526 1560
Size: 526 1034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1415 1560
Size: 1415 145
BtsI GCAGTG_nn'
Cuts at: 0 457 989 1082 1560
Size: 457 532 93 478
Fragments arranged by size:
532 478 457 93
DrdI GACnn_nn'nnGTC
Cuts at: 0 811 1560
Size: 811 749
EaeI y'GGCC_r
Cuts at: 0 592 710 1560
Size: 592 118 850
Fragments arranged by size:
850 592 118
EarI CTCTTCn'nnn_
Cuts at: 0 385 984 1525 1560
Size: 385 599 541 35
Fragments arranged by size:
599 541 385 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 743 1560
Size: 743 817
EcoICRI GAG'CTC
Cuts at: 0 369 455 1029 1560
Size: 369 86 574 531
Fragments arranged by size:
574 531 369 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1349 1560
Size: 358 991 211
Fragments arranged by size:
991 358 211
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 730 1560
Size: 337 393 830
Fragments arranged by size:
830 393 337
EcoO109I rG'GnC_Cy
Cuts at: 0 966 1560
Size: 966 594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 1560
Size: 291 1269
HaeII r_GCGC'y
Cuts at: 0 390 891 1560
Size: 390 501 669
Fragments arranged by size:
669 501 390
HincII GTy'rAC
Cuts at: 0 398 1016 1560
Size: 398 618 544
Fragments arranged by size:
618 544 398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1560
Size: 176 1384
MscI TGG'CCA
Cuts at: 0 712 1560
Size: 712 848
MslI CAynn'nnrTG
Cuts at: 0 181 928 1271 1560
Size: 181 747 343 289
Fragments arranged by size:
747 343 289 181
MspA1I CmG'CkG
Cuts at: 0 479 528 591 1369 1381 1525 1560
Size: 479 49 63 778 12 144 35
Fragments arranged by size:
778 479 144 63 49 35 12
NsiI A_TGCA'T
Cuts at: 0 848 1560
Size: 848 712
PmlI CAC'GTG
Cuts at: 0 1269 1560
Size: 1269 291
PspOMI G'GGCC_C
Cuts at: 0 1471 1560
Size: 1471 89
PstI C_TGCA'G
Cuts at: 0 464 1560
Size: 464 1096
PvuII CAG'CTG
Cuts at: 0 1369 1381 1525 1560
Size: 1369 12 144 35
Fragments arranged by size:
1369 144 35 12
SacI G_AGCT'C
Cuts at: 0 371 457 1031 1560
Size: 371 86 574 529
Fragments arranged by size:
574 529 371 86
SacII CC_GC'GG
Cuts at: 0 529 1560
Size: 529 1031
SapI GCTCTTCn'nnn_
Cuts at: 0 385 1560
Size: 385 1175
SexAI A'CCwGG_T
Cuts at: 0 1217 1560
Size: 1217 343
SfcI C'TryA_G
Cuts at: 0 460 556 1560
Size: 460 96 1004
Fragments arranged by size:
1004 460 96
SmaI CCC'GGG
Cuts at: 0 1250 1560
Size: 1250 310
SmlI C'TyrA_G
Cuts at: 0 199 1560
Size: 199 1361
StyI C'CwwG_G
Cuts at: 0 891 1560
Size: 891 669
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1261 1309 1560
Size: 790 471 48 251
Fragments arranged by size:
790 471 251 48
TatI w'GTAC_w
Cuts at: 0 203 212 1560
Size: 203 9 1348
Fragments arranged by size:
1348 203 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 483 1560
Size: 180 303 1077
Fragments arranged by size:
1077 303 180
XmaI C'CCGG_G
Cuts at: 0 1248 1560
Size: 1248 312
XmnI GAAnn'nnTTC
Cuts at: 0 274 1560
Size: 274 1286
Enzymes that do cut:
AccI AgeI AleI AlfI AlwNI ApaI AvaI BaeI
BamHI BanI BanII BbvCI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII HincII
MmeI MscI MslI MspA1I NsiI PmlI PspOMI PstI
PvuII SacI SacII SapI SexAI SfcI SmaI SmlI
StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BbeI BbsI BcgI BciVI BclI BmgBI BmtI BsaHI
BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BstAPI
BstBI BstZ17I ClaI CspCI DraI DraIII EagI EcoRI
EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI ZraI