Restriction map for
pF1KB0023
(Linear) (Six-base) MAPSORT of: KIBB0023.seq Check: 6445 from: 1 to: 6345
KIBB0023.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2767 4638 6345
Size: 2767 1871 1707
Fragments arranged by size:
2767 1871 1707
AatII G_ACGT'C
Cuts at: 0 5387 6345
Size: 5387 958
AccI GT'mk_AC
Cuts at: 0 51 6345
Size: 51 6294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 515 676 907 925 1132 1591 1699
Size: 158 357 161 231 18 207 459 108
Cuts at: 1699 1855 2236 2366 3289 3635 3905 4339 4735
Size: 156 381 130 923 346 270 434 396
Cuts at: 4735 4970 5011 5821 6132 6345
Size: 235 41 810 311 213
Fragments arranged by size:
923 810 459 434 396 381 357 346
311 270 235 231 213 207 161 158
156 130 108 41 18
AfeI AGC'GCT
Cuts at: 0 2897 6345
Size: 2897 3448
AflII C'TTAA_G
Cuts at: 0 1785 6345
Size: 1785 4560
AflIII A'CryG_T
Cuts at: 0 54 6345
Size: 54 6291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 1757 4142 6345
Size: 217 1540 2385 2203
Fragments arranged by size:
2385 2203 1540 217
AlwNI CAG_nnn'CTG
Cuts at: 0 344 903 1389 1752 2544 3723 3792 3942
Size: 344 559 486 363 792 1179 69 150
Cuts at: 3942 4065 4107 4275 4392 4713 5286 6345
Size: 123 42 168 117 321 573 1059
Fragments arranged by size:
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321 168 150 123 117 69 42
ApaI G_GGCC'C
Cuts at: 0 1482 2685 4206 5685 5992 6345
Size: 1482 1203 1521 1479 307 353
Fragments arranged by size:
1521 1482 1479 1203 353 307
ApoI r'AATT_y
Cuts at: 0 365 394 5095 6345
Size: 365 29 4701 1250
Fragments arranged by size:
4701 1250 365 29
AvaI C'yCGr_G
Cuts at: 0 17 1889 2801 2845 3448 3467 3641 4042
Size: 17 1872 912 44 603 19 174 401
Cuts at: 4042 4415 4505 5397 6047 6320 6345
Size: 373 90 892 650 273 25
Fragments arranged by size:
1872 912 892 650 603 401 373 273
174 90 44 25 19 17
BanI G'GyrC_C
Cuts at: 0 2482 4540 5242 5845 6131 6310 6345
Size: 2482 2058 702 603 286 179 35
Fragments arranged by size:
2482 2058 702 603 286 179 35
BanII G_rGCy'C
Cuts at: 0 328 503 824 1097 1420 1482 1520 2594
Size: 328 175 321 273 323 62 38 1074
Cuts at: 2594 2630 2685 3321 4206 5135 5685 5981 5992
Size: 36 55 636 885 929 550 296 11
Cuts at: 5992 6345
Size: 353
Fragments arranged by size:
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321 296 273 175 62 55 38 36
11
BbeI G_GCGC'C
Cuts at: 0 2486 6135 6345
Size: 2486 3649 210
Fragments arranged by size:
3649 2486 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 913 4999 5379 5809 6345
Size: 913 4086 380 430 536
Fragments arranged by size:
4086 913 536 430 380
BbvCI CC'TCA_GC
Cuts at: 0 1432 4475 6345
Size: 1432 3043 1870
Fragments arranged by size:
3043 1870 1432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 227 724 758 5761 5795 6345
Size: 193 34 497 34 5003 34 550
Fragments arranged by size:
5003 550 497 193 34 34 34
BclI T'GATC_A
Cuts at: 0 1400 6345
Size: 1400 4945
BglI GCCn_nnn'nGGC
Cuts at: 0 1468 1498 2335 2641 2850 3469 3637 4120
Size: 1468 30 837 306 209 619 168 483
Cuts at: 4120 6345
Size: 2225
Fragments arranged by size:
2225 1468 837 619 483 306 209 168
30
BlpI GC'TnA_GC
Cuts at: 0 1096 1355 1666 1942 2624 3455 3473 3956
Size: 1096 259 311 276 682 831 18 483
Cuts at: 3956 4088 4268 4309 4820 5141 5611 6345
Size: 132 180 41 511 321 470 734
Fragments arranged by size:
1096 831 734 682 511 483 470 321
311 276 259 180 132 41 18
Bme1580I G_kGCm'C
Cuts at: 0 1482 1902 2685 3048 3539 4206 5685 5716
Size: 1482 420 783 363 491 667 1479 31
Cuts at: 5716 5791 5848 5935 5992 6345
Size: 75 57 87 57 353
Fragments arranged by size:
1482 1479 783 667 491 420 363 353
87 75 57 57 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 354 4788 6345
Size: 354 4434 1557
Fragments arranged by size:
4434 1557 354
BmtI G_CTAG'C
Cuts at: 0 712 1606 5540 6345
Size: 712 894 3934 805
Fragments arranged by size:
3934 894 805 712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1479 3480 3963 6345
Size: 1479 2001 483 2382
Fragments arranged by size:
2382 2001 1479 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 584 718 724 838 1105 1336 1474
Size: 73 511 134 6 114 267 231 138
Cuts at: 1474 1837 1846 1870 1948 2083 2095 2320 2578
Size: 363 9 24 78 135 12 225 258
Cuts at: 2578 2638 3085 3235 3259 3385 3511 3745 3814
Size: 60 447 150 24 126 126 234 69
Cuts at: 3814 4174 4534 4579 4750 4933 5068 5276 5329
Size: 360 360 45 171 183 135 208 53
Cuts at: 5329 5377 5672 5839 5894 6089 6345
Size: 48 295 167 55 195 256
Fragments arranged by size:
511 447 363 360 360 295 267 258
256 234 231 225 208 195 183 171
167 150 138 135 135 134 126 126
114 78 73 69 60 55 53 48
45 24 24 12 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 1432 3312 3552 4475 4730 5732 5836 6345
Size: 1432 1880 240 923 255 1002 104 509
Fragments arranged by size:
1880 1432 1002 923 509 255 240 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1792 3109 5178 6345
Size: 853 939 1317 2069 1167
Fragments arranged by size:
2069 1317 1167 939 853
BsaI GGTCTCn'nnnn_
Cuts at: 0 956 2218 2752 4456 5078 5870 5881 5973
Size: 956 1262 534 1704 622 792 11 92
Cuts at: 5973 6044 6345
Size: 71 301
Fragments arranged by size:
1704 1262 956 792 622 534 301 92
71 11
BsaAI yAC'GTr
Cuts at: 0 57 4450 6345
Size: 57 4393 1895
Fragments arranged by size:
4393 1895 57
BsaHI Gr'CG_yC
Cuts at: 0 2483 2871 5384 6132 6345
Size: 2483 388 2513 748 213
Fragments arranged by size:
2513 2483 748 388 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1598 1628 1681 1711 2461 2491 3685 3715
Size: 1598 30 53 30 750 30 1194 30
Cuts at: 3715 6345
Size: 2630
Fragments arranged by size:
2630 1598 1194 750 53 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 555 718 850 967 1051 1783 2173 2482
Size: 555 163 132 117 84 732 390 309
Cuts at: 2482 3004 3529 3706 3709 3811 3889 3910 3982
Size: 522 525 177 3 102 78 21 72
Cuts at: 3982 4162 4360 4576 5203 5544 5875 6345
Size: 180 198 216 627 341 331 470
Fragments arranged by size:
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331 309 216 198 180 177 163 132
117 102 84 78 72 21 3
BseYI C'CCAG_C
Cuts at: 0 1079 1184 1224 1598 1745 1922 2284 2384
Size: 1079 105 40 374 147 177 362 100
Cuts at: 2384 3972 4174 4205 4359 4757 4772 4962 4984
Size: 1588 202 31 154 398 15 190 22
Cuts at: 4984 5147 5231 5279 6345
Size: 163 84 48 1066
Fragments arranged by size:
1588 1079 1066 398 374 362 202 190
177 163 154 147 105 100 84 48
40 31 22 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 213 233 3901 4501 4720 6345
Size: 11 202 20 3668 600 219 1625
Fragments arranged by size:
3668 1625 600 219 202 20 11
BsiHKAI G_wGCw'C
Cuts at: 0 328 503 1043 1097 1420 1520 2594 2630
Size: 328 175 540 54 323 100 1074 36
Cuts at: 2630 2652 3302 3489 4049 4501 4826 6079 6345
Size: 22 650 187 560 452 325 1253 266
Fragments arranged by size:
1253 1074 650 560 540 452 328 325
323 266 187 175 100 54 36 22
BsiWI C'GTAC_G
Cuts at: 0 4446 6345
Size: 4446 1899
BsmI GAATG_Cn'
Cuts at: 0 241 6345
Size: 241 6104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 642 5375 6345
Size: 642 4733 970
Fragments arranged by size:
4733 970 642
Bsp1286I G_dGCh'C
Cuts at: 0 328 503 824 1043 1097 1420 1482 1520
Size: 328 175 321 219 54 323 62 38
Cuts at: 1520 1902 2594 2630 2652 2685 3048 3302 3321
Size: 382 692 36 22 33 363 254 19
Cuts at: 3321 3489 3539 4049 4206 4501 4826 5135 5685
Size: 168 50 510 157 295 325 309 550
Cuts at: 5685 5716 5791 5848 5935 5981 5992 6079 6345
Size: 31 75 57 87 46 11 87 266
Fragments arranged by size:
692 550 510 382 363 328 325 323
321 309 295 266 254 219 175 168
157 87 87 75 62 57 54 50
46 38 36 33 31 22 19 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1418 2649 2767 3938 4638 5023 5815 6345
Size: 1418 1231 118 1171 700 385 792 530
Fragments arranged by size:
1418 1231 1171 792 700 530 385 118
BsrBI CCG'CTC
Cuts at: 0 1359 1794 2445 2913 3036 3459 3477 4368
Size: 1359 435 651 468 123 423 18 891
Cuts at: 4368 5414 5419 6345
Size: 1046 5 926
Fragments arranged by size:
1359 1046 926 891 651 468 435 423
123 18 5
BsrDI GCAATG_nn'
Cuts at: 0 2940 3409 5756 6266 6345
Size: 2940 469 2347 510 79
Fragments arranged by size:
2940 2347 510 469 79
BsrFI r'CCGG_y
Cuts at: 0 165 2603 3647 4382 6128 6345
Size: 165 2438 1044 735 1746 217
Fragments arranged by size:
2438 1746 1044 735 217 165
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4335 5059 5113 6345
Size: 4335 724 54 1232
Fragments arranged by size:
4335 1232 724 54
BstBI TT'CG_AA
Cuts at: 0 1370 6345
Size: 1370 4975
BstEII G'GTnAC_C
Cuts at: 0 2136 6345
Size: 2136 4209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 1474 5992 6345
Size: 816 658 4518 353
Fragments arranged by size:
4518 816 658 353
BstYI r'GATC_y
Cuts at: 0 5175 6345
Size: 5175 1170
BtgI C'CryG_G
Cuts at: 0 129 611 2375 6345
Size: 129 482 1764 3970
Fragments arranged by size:
3970 1764 482 129
BtsI GCAGTG_nn'
Cuts at: 0 898 1757 3370 4499 4598 6249 6293 6345
Size: 898 859 1613 1129 99 1651 44 52
Fragments arranged by size:
1651 1613 1129 898 859 99 52 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3189 3224 6345
Size: 3189 35 3121
Fragments arranged by size:
3189 3121 35
DraI TTT'AAA
Cuts at: 0 5120 6345
Size: 5120 1225
DraIII CAC_nnn'GTG
Cuts at: 0 1656 6345
Size: 1656 4689
EaeI y'GGCC_r
Cuts at: 0 806 1754 2642 3074 3197 3767 4380 6345
Size: 806 948 888 432 123 570 613 1965
Fragments arranged by size:
1965 948 888 806 613 570 432 123
EarI CTCTTCn'nnn_
Cuts at: 0 134 231 491 573 910 1124 1442 2150
Size: 134 97 260 82 337 214 318 708
Cuts at: 2150 2183 2270 2852 3775 4577 5471 6345
Size: 33 87 582 923 802 894 874
Fragments arranged by size:
923 894 874 802 708 582 337 318
260 214 134 97 87 82 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1636 6345
Size: 1636 4709
EcoICRI GAG'CTC
Cuts at: 0 326 501 1095 1418 1518 2592 2628 6345
Size: 326 175 594 323 100 1074 36 3717
Fragments arranged by size:
3717 1074 594 326 323 175 100 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 158 515 584 676 718 724 838
Size: 73 85 357 69 92 42 6 114
Cuts at: 838 907 925 1105 1132 1336 1474 1591 1699
Size: 69 18 180 27 204 138 117 108
Cuts at: 1699 1837 1846 1855 1870 1948 2083 2095 2236
Size: 138 9 9 15 78 135 12 141
Cuts at: 2236 2320 2366 2578 2638 3085 3235 3259 3289
Size: 84 46 212 60 447 150 24 30
Cuts at: 3289 3385 3511 3635 3745 3814 3905 4174 4339
Size: 96 126 124 110 69 91 269 165
Cuts at: 4339 4534 4579 4735 4750 4933 4970 5011 5068
Size: 195 45 156 15 183 37 41 57
Cuts at: 5068 5276 5329 5377 5672 5821 5839 5894 6089
Size: 208 53 48 295 149 18 55 195
Cuts at: 6089 6132 6345
Size: 43 213
Fragments arranged by size:
447 357 295 269 213 212 208 204
195 195 183 180 165 156 150 149
141 138 138 135 126 124 117 114
110 108 96 92 91 85 84 78
73 69 69 69 60 57 55 53
48 46 45 43 42 41 37 30
27 24 18 18 15 15 12 9
9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 1435 1785 2155 3067 3145 3727 3814
Size: 430 1005 350 370 912 78 582 87
Cuts at: 3814 3892 3934 6220 6345
Size: 78 42 2286 125
Fragments arranged by size:
2286 1005 912 582 430 370 350 125
87 78 78 42
EcoO109I rG'GnC_Cy
Cuts at: 0 435 900 1478 1641 2115 2681 3355 3720
Size: 435 465 578 163 474 566 674 365
Cuts at: 3720 4023 4062 4554 5195 5349 5681 5682 5988
Size: 303 39 492 641 154 332 1 306
Cuts at: 5988 5989 6213 6345
Size: 1 224 132
Fragments arranged by size:
674 641 578 566 492 474 465 435
365 332 306 303 224 163 154 132
39 1 1
EcoRV GAT'ATC
Cuts at: 0 621 6345
Size: 621 5724
FspI TGC'GCA
Cuts at: 0 2770 5041 6345
Size: 2770 2271 1304
Fragments arranged by size:
2770 2271 1304
HaeII r_GCGC'y
Cuts at: 0 2486 2899 4764 5286 5492 6135 6290 6345
Size: 2486 413 1865 522 206 643 155 55
Fragments arranged by size:
2486 1865 643 522 413 206 155 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 2689 2721 3289 3321 3685 3717
Size: 137 32 2520 32 568 32 364 32
Cuts at: 3717 5796 5828 6345
Size: 2079 32 517
Fragments arranged by size:
2520 2079 568 517 364 137 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1487 6345
Size: 1487 4858
HindIII A'AGCT_T
Cuts at: 0 2689 3202 6345
Size: 2689 513 3143
Fragments arranged by size:
3143 2689 513
KasI G'GCGC_C
Cuts at: 0 2482 6131 6345
Size: 2482 3649 214
Fragments arranged by size:
3649 2482 214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1451 1641 4385 6345
Size: 1451 190 2744 1960
Fragments arranged by size:
2744 1960 1451 190
MscI TGG'CCA
Cuts at: 0 1756 2644 3199 6345
Size: 1756 888 555 3146
Fragments arranged by size:
3146 1756 888 555
MslI CAynn'nnrTG
Cuts at: 0 4862 5452 6345
Size: 4862 590 893
Fragments arranged by size:
4862 893 590
MspA1I CmG'CkG
Cuts at: 0 1083 1167 1188 1242 1389 1815 1926 2037
Size: 1083 84 21 54 147 426 111 111
Cuts at: 2037 2395 2502 3279 3912 4107 4126 4174 4209
Size: 358 107 777 633 195 19 48 35
Cuts at: 4209 4275 4308 4572 4776 4819 4902 4920 5151
Size: 66 33 264 204 43 83 18 231
Cuts at: 5151 5739 6023 6345
Size: 588 284 322
Fragments arranged by size:
1083 777 633 588 426 358 322 284
264 231 204 195 147 111 111 107
84 83 66 54 48 43 35 33
21 19 18
NaeI GCC'GGC
Cuts at: 0 2605 3649 4384 6345
Size: 2605 1044 735 1961
Fragments arranged by size:
2605 1961 1044 735
NarI GG'CG_CC
Cuts at: 0 2483 6132 6345
Size: 2483 3649 213
Fragments arranged by size:
3649 2483 213
NcoI C'CATG_G
Cuts at: 0 129 611 2375 6345
Size: 129 482 1764 3970
Fragments arranged by size:
3970 1764 482 129
NgoMIV G'CCGG_C
Cuts at: 0 2603 3647 4382 6345
Size: 2603 1044 735 1963
Fragments arranged by size:
2603 1963 1044 735
NheI G'CTAG_C
Cuts at: 0 708 1602 5536 6345
Size: 708 894 3934 809
Fragments arranged by size:
3934 894 809 708
NspI r_CATG'y
Cuts at: 0 4317 5910 6345
Size: 4317 1593 435
Fragments arranged by size:
4317 1593 435
PasI CC'CwG_GG
Cuts at: 0 338 1638 5376 6345
Size: 338 1300 3738 969
Fragments arranged by size:
3738 1300 969 338
PflMI CCAn_nnn'nTGG
Cuts at: 0 344 1656 1752 3195 3723 3792 4437 6141
Size: 344 1312 96 1443 528 69 645 1704
Cuts at: 6141 6345
Size: 204
Fragments arranged by size:
1704 1443 1312 645 528 344 204 96
69
PfoI T'CCnGG_A
Cuts at: 0 89 2099 2492 3722 3890 4556 4577 5390
Size: 89 2010 393 1230 168 666 21 813
Cuts at: 5390 5688 6345
Size: 298 657
Fragments arranged by size:
2010 1230 813 666 657 393 298 168
89 21
PmlI CAC'GTG
Cuts at: 0 57 6345
Size: 57 6288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3431 3463 6345
Size: 3431 32 2882
Fragments arranged by size:
3431 2882 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 900 3720 4554 5195 6345
Size: 435 465 2820 834 641 1150
Fragments arranged by size:
2820 1150 834 641 465 435
PshAI GACnn'nnGTC
Cuts at: 0 2347 5399 6345
Size: 2347 3052 946
Fragments arranged by size:
3052 2347 946
PspOMI G'GGCC_C
Cuts at: 0 1478 2681 4202 5681 5988 6345
Size: 1478 1203 1521 1479 307 357
Fragments arranged by size:
1521 1479 1478 1203 357 307
PspXI vC'TCGA_Gb
Cuts at: 0 3448 4042 6320 6345
Size: 3448 594 2278 25
Fragments arranged by size:
3448 2278 594 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5689 5721 6345
Size: 5689 32 624
Fragments arranged by size:
5689 624 32
PstI C_TGCA'G
Cuts at: 0 86 100 206 629 1004 1112 1193 1415
Size: 86 14 106 423 375 108 81 222
Cuts at: 1415 1767 1862 2178 2549 2717 2884 2903 3104
Size: 352 95 316 371 168 167 19 201
Cuts at: 3104 3503 3818 3852 4040 4112 4838 4895 4925
Size: 399 315 34 188 72 726 57 30
Cuts at: 4925 5771 6345
Size: 846 574
Fragments arranged by size:
846 726 574 423 399 375 371 352
316 315 222 201 188 168 167 108
106 95 86 81 72 57 34 30
19 14
PvuII CAG'CTG
Cuts at: 0 1083 1167 1188 1242 1389 1815 2037 2395
Size: 1083 84 21 54 147 426 222 358
Cuts at: 2395 2502 3279 4107 4275 4308 4776 4819 4902
Size: 107 777 828 168 33 468 43 83
Cuts at: 4902 4920 5151 5739 6023 6345
Size: 18 231 588 284 322
Fragments arranged by size:
1083 828 777 588 468 426 358 322
284 231 222 168 147 107 84 83
54 43 33 21 18
SacI G_AGCT'C
Cuts at: 0 328 503 1097 1420 1520 2594 2630 6345
Size: 328 175 594 323 100 1074 36 3715
Fragments arranged by size:
3715 1074 594 328 323 175 100 36
SanDI GG'GwC_CC
Cuts at: 0 5195 6345
Size: 5195 1150
SapI GCTCTTCn'nnn_
Cuts at: 0 491 910 1442 2183 2852 3775 5471 6345
Size: 491 419 532 741 669 923 1696 874
Fragments arranged by size:
1696 923 874 741 669 532 491 419
SbfI CC_TGCA'GG
Cuts at: 0 1112 6345
Size: 1112 5233
SexAI A'CCwGG_T
Cuts at: 0 1652 6345
Size: 1652 4693
SfcI C'TryA_G
Cuts at: 0 82 96 202 451 550 625 1000 1009
Size: 82 14 106 249 99 75 375 9
Cuts at: 1009 1108 1189 1411 1763 1858 2174 2545 2713
Size: 99 81 222 352 95 316 371 168
Cuts at: 2713 2880 2899 3100 3499 3814 3848 4036 4108
Size: 167 19 201 399 315 34 188 72
Cuts at: 4108 4834 4891 4921 4975 5505 5767 6345
Size: 726 57 30 54 530 262 578
Fragments arranged by size:
726 578 530 399 375 371 352 316
315 262 249 222 201 188 168 167
106 99 99 95 82 81 75 72
57 54 34 30 19 14 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3637 4120 6345
Size: 3637 483 2225
Fragments arranged by size:
3637 2225 483
SfoI GGC'GCC
Cuts at: 0 2484 6133 6345
Size: 2484 3649 212
Fragments arranged by size:
3649 2484 212
SmaI CCC'GGG
Cuts at: 0 19 1891 2803 3469 3643 4417 4507 6345
Size: 19 1872 912 666 174 774 90 1838
Fragments arranged by size:
1872 1838 912 774 666 174 90 19
SmlI C'TyrA_G
Cuts at: 0 832 1785 1807 3088 3448 4042 5157 6320
Size: 832 953 22 1281 360 594 1115 1163
Cuts at: 6320 6345
Size: 25
Fragments arranged by size:
1281 1163 1115 953 832 594 360 25
22
SphI G_CATG'C
Cuts at: 0 4317 5910 6345
Size: 4317 1593 435
Fragments arranged by size:
4317 1593 435
StuI AGG'CCT
Cuts at: 0 1462 1633 1780 1969 5829 6345
Size: 1462 171 147 189 3860 516
Fragments arranged by size:
3860 1462 516 189 171 147
StyI C'CwwG_G
Cuts at: 0 129 611 824 1442 2141 2375 2762 3590
Size: 129 482 213 618 699 234 387 828
Cuts at: 3590 4377 4544 4997 5294 6221 6326 6345
Size: 787 167 453 297 927 105 19
Fragments arranged by size:
927 828 787 699 618 482 453 387
297 234 213 167 129 105 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 30 604 1216 4351 6068 6180 6345
Size: 30 574 612 3135 1717 112 165
Fragments arranged by size:
3135 1717 612 574 165 112 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 1598 1630 6345
Size: 175 32 1391 32 4715
Fragments arranged by size:
4715 1391 175 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1449 2742 4213 5989 6345
Size: 1449 1293 1471 1776 356
Fragments arranged by size:
1776 1471 1449 1293 356
XhoI C'TCGA_G
Cuts at: 0 3448 4042 6320 6345
Size: 3448 594 2278 25
Fragments arranged by size:
3448 2278 594 25
XmaI C'CCGG_G
Cuts at: 0 17 1889 2801 3467 3641 4415 4505 6345
Size: 17 1872 912 666 174 774 90 1840
Fragments arranged by size:
1872 1840 912 774 666 174 90 17
XmnI GAAnn'nnTTC
Cuts at: 0 1145 1158 4006 5701 6345
Size: 1145 13 2848 1695 644
Fragments arranged by size:
2848 1695 1145 644 13
ZraI GAC'GTC
Cuts at: 0 5385 6345
Size: 5385 960
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AlfI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI
BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuII SacI SanDI SapI SbfI SexAI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TaqII
TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AhdI AleI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BglII
BmgBI BsaBI BsaWI BsiEI BspEI BspHI BsrGI BssHII
BssSI BstZ17I Bsu36I BtgZI ClaI DrdI EagI EcoRI
FalI FseI FspAI HpaI KpnI MfeI MluI NdeI
NotI NruI NsiI PacI PciI PmeI PsiI PvuI
RsrII SacII SalI ScaI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI