Restriction map for
pF1KSDB0024
(Linear) (Six-base) MAPSORT of: KIBB0024.seq Check: 6684 from: 1 to: 3741
KIBB0024.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 3731 3741
Size: 3731 10
Acc65I G'GTAC_C
Cuts at: 0 2101 3741
Size: 2101 1640
AclI AA'CG_TT
Cuts at: 0 867 2936 3741
Size: 867 2069 805
Fragments arranged by size:
2069 867 805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1241 1289 1942 2054 2585 2885 2912
Size: 172 1069 48 653 112 531 300 27
Cuts at: 2912 3555 3741
Size: 643 186
Fragments arranged by size:
1069 653 643 531 300 186 172 112
48 27
AflIII A'CryG_T
Cuts at: 0 3311 3741
Size: 3311 430
AhdI GACnn_n'nnGTC
Cuts at: 0 748 1695 3741
Size: 748 947 2046
Fragments arranged by size:
2046 947 748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1768 2860 3741
Size: 1768 1092 881
Fragments arranged by size:
1768 1092 881
AlwNI CAG_nnn'CTG
Cuts at: 0 3039 3741
Size: 3039 702
ApaI G_GGCC'C
Cuts at: 0 3015 3741
Size: 3015 726
ApoI r'AATT_y
Cuts at: 0 304 2079 3741
Size: 304 1775 1662
Fragments arranged by size:
1775 1662 304
AseI AT'TA_AT
Cuts at: 0 26 3741
Size: 26 3715
AvaI C'yCGr_G
Cuts at: 0 887 2425 3741
Size: 887 1538 1316
Fragments arranged by size:
1538 1316 887
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2085 2118 3741
Size: 2085 33 1623
Fragments arranged by size:
2085 1623 33
BamHI G'GATC_C
Cuts at: 0 703 1678 3741
Size: 703 975 2063
Fragments arranged by size:
2063 975 703
BanI G'GyrC_C
Cuts at: 0 2101 2782 3741
Size: 2101 681 959
Fragments arranged by size:
2101 959 681
BanII G_rGCy'C
Cuts at: 0 2343 3015 3741
Size: 2343 672 726
Fragments arranged by size:
2343 726 672
BbsI GAAGACnn'nnnn_
Cuts at: 0 124 1734 2486 2634 3285 3741
Size: 124 1610 752 148 651 456
Fragments arranged by size:
1610 752 651 456 148 124
BbvCI CC'TCA_GC
Cuts at: 0 1653 3741
Size: 1653 2088
BciVI GTATCCnnnnn_n'
Cuts at: 0 510 1392 3341 3741
Size: 510 882 1949 400
Fragments arranged by size:
1949 882 510 400
BglI GCCn_nnn'nGGC
Cuts at: 0 2072 3741
Size: 2072 1669
BglII A'GATC_T
Cuts at: 0 1576 3171 3741
Size: 1576 1595 570
Fragments arranged by size:
1595 1576 570
BlpI GC'TnA_GC
Cuts at: 0 3447 3741
Size: 3447 294
Bme1580I G_kGCm'C
Cuts at: 0 3015 3741
Size: 3015 726
BmgBI CAC'GTC
Cuts at: 0 3312 3577 3741
Size: 3312 265 164
Fragments arranged by size:
3312 265 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 477 2150 2682 3009 3741
Size: 477 1673 532 327 732
Fragments arranged by size:
1673 732 532 477 327
BmtI G_CTAG'C
Cuts at: 0 1174 1948 3741
Size: 1174 774 1793
Fragments arranged by size:
1793 1174 774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 232 3741
Size: 232 3509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 433 2960 3609 3741
Size: 202 231 2527 649 132
Fragments arranged by size:
2527 649 231 202 132
Bpu10I CC'TnA_GC
Cuts at: 0 1653 2576 3741
Size: 1653 923 1165
Fragments arranged by size:
1653 1165 923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1186 2431 2594 3409 3741
Size: 505 681 1245 163 815 332
Fragments arranged by size:
1245 815 681 505 332 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 3741
Size: 145 3596
BsaAI yAC'GTr
Cuts at: 0 2751 3741
Size: 2751 990
BsaBI GATnn'nnATC
Cuts at: 0 3056 3741
Size: 3056 685
BsaHI Gr'CG_yC
Cuts at: 0 3719 3741
Size: 3719 22
BsaWI w'CCGG_w
Cuts at: 0 300 3021 3741
Size: 300 2721 720
Fragments arranged by size:
2721 720 300
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 2307 2337 3741
Size: 67 30 2210 30 1404
Fragments arranged by size:
2210 1404 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1390 2312 2315 2897 3596 3741
Size: 1390 922 3 582 699 145
Fragments arranged by size:
1390 922 699 582 145 3
BseYI C'CCAG_C
Cuts at: 0 176 3008 3088 3741
Size: 176 2832 80 653
Fragments arranged by size:
2832 653 176 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 593 3741
Size: 296 297 3148
Fragments arranged by size:
3148 297 296
BsiHKAI G_wGCw'C
Cuts at: 0 548 1050 3741
Size: 548 502 2691
Fragments arranged by size:
2691 548 502
BsmI GAATG_Cn'
Cuts at: 0 2741 3133 3741
Size: 2741 392 608
Fragments arranged by size:
2741 608 392
Bsp1286I G_dGCh'C
Cuts at: 0 548 1050 2343 3015 3741
Size: 548 502 1293 672 726
Fragments arranged by size:
1293 726 672 548 502
BspHI T'CATG_A
Cuts at: 0 2222 2949 3060 3741
Size: 2222 727 111 681
Fragments arranged by size:
2222 727 681 111
BsrBI CCG'CTC
Cuts at: 0 3395 3741
Size: 3395 346
BsrDI GCAATG_nn'
Cuts at: 0 3333 3741
Size: 3333 408
BssSI C'ACGA_G
Cuts at: 0 1792 3608 3741
Size: 1792 1816 133
Fragments arranged by size:
1816 1792 133
BstEII G'GTnAC_C
Cuts at: 0 3098 3741
Size: 3098 643
BstXI CCAn_nnnn'nTGG
Cuts at: 0 67 2205 2695 3741
Size: 67 2138 490 1046
Fragments arranged by size:
2138 1046 490 67
BstYI r'GATC_y
Cuts at: 0 703 930 1576 1678 1905 2813 3171 3741
Size: 703 227 646 102 227 908 358 570
Fragments arranged by size:
908 703 646 570 358 227 227 102
Bsu36I CC'TnA_GG
Cuts at: 0 492 3741
Size: 492 3249
BtgI C'CryG_G
Cuts at: 0 986 3741
Size: 986 2755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2117 2833 3741
Size: 2117 716 908
Fragments arranged by size:
2117 908 716
BtsI GCAGTG_nn'
Cuts at: 0 72 2227 2842 3741
Size: 72 2155 615 899
Fragments arranged by size:
2155 899 615 72
DraI TTT'AAA
Cuts at: 0 3188 3741
Size: 3188 553
EaeI y'GGCC_r
Cuts at: 0 2138 3741
Size: 2138 1603
EarI CTCTTCn'nnn_
Cuts at: 0 1130 1265 1319 1752 2465 2770 3741
Size: 1130 135 54 433 713 305 971
Fragments arranged by size:
1130 971 713 433 305 135 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 202 433 1241 1289 1942 2054 2585
Size: 172 30 231 808 48 653 112 531
Cuts at: 2585 2885 2912 2960 3555 3609 3741
Size: 300 27 48 595 54 132
Fragments arranged by size:
808 653 595 531 300 231 172 132
112 54 48 48 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 244 1261 3591 3741
Size: 244 1017 2330 150
Fragments arranged by size:
2330 1017 244 150
EcoO109I rG'GnC_Cy
Cuts at: 0 56 3641 3741
Size: 56 3585 100
Fragments arranged by size:
3585 100 56
EcoRV GAT'ATC
Cuts at: 0 252 3741
Size: 252 3489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 844 1180 2476 2551 3741
Size: 844 336 1296 75 1190
Fragments arranged by size:
1296 1190 844 336 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 196 228 241 273 734 766
Size: 67 32 97 32 13 32 461 32
Cuts at: 766 1681 1713 1897 1929 2235 2267 2343 2375
Size: 915 32 184 32 306 32 76 32
Cuts at: 2375 2855 2887 3741
Size: 480 32 854
Fragments arranged by size:
915 854 480 461 306 184 97 76
67 32 32 32 32 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 1318 3674 3741
Size: 1318 2356 67
Fragments arranged by size:
2356 1318 67
HpaI GTT'AAC
Cuts at: 0 3674 3741
Size: 3674 67
KpnI G_GTAC'C
Cuts at: 0 2105 3741
Size: 2105 1636
MfeI C'AATT_G
Cuts at: 0 1591 1966 3356 3741
Size: 1591 375 1390 385
Fragments arranged by size:
1591 1390 385 375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2935 3493 3741
Size: 2935 558 248
Fragments arranged by size:
2935 558 248
MscI TGG'CCA
Cuts at: 0 2140 3741
Size: 2140 1601
MslI CAynn'nnrTG
Cuts at: 0 40 1750 1855 1931 2381 3741
Size: 40 1710 105 76 450 1360
Fragments arranged by size:
1710 1360 450 105 76 40
MspA1I CmG'CkG
Cuts at: 0 99 1657 3661 3741
Size: 99 1558 2004 80
Fragments arranged by size:
2004 1558 99 80
NcoI C'CATG_G
Cuts at: 0 986 3741
Size: 986 2755
NheI G'CTAG_C
Cuts at: 0 1170 1944 3741
Size: 1170 774 1797
Fragments arranged by size:
1797 1170 774
PasI CC'CwG_GG
Cuts at: 0 2586 3528 3741
Size: 2586 942 213
Fragments arranged by size:
2586 942 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 2880 3741
Size: 2880 861
PfoI T'CCnGG_A
Cuts at: 0 2873 3741
Size: 2873 868
PpuMI rG'GwC_Cy
Cuts at: 0 56 3641 3741
Size: 56 3585 100
Fragments arranged by size:
3585 100 56
PspOMI G'GGCC_C
Cuts at: 0 3011 3741
Size: 3011 730
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1613 1645 3741
Size: 1613 32 2096
Fragments arranged by size:
2096 1613 32
PstI C_TGCA'G
Cuts at: 0 340 2574 3741
Size: 340 2234 1167
Fragments arranged by size:
2234 1167 340
PvuII CAG'CTG
Cuts at: 0 99 1657 3661 3741
Size: 99 1558 2004 80
Fragments arranged by size:
2004 1558 99 80
SanDI GG'GwC_CC
Cuts at: 0 56 3741
Size: 56 3685
SbfI CC_TGCA'GG
Cuts at: 0 340 3741
Size: 340 3401
ScaI AGT'ACT
Cuts at: 0 1823 3741
Size: 1823 1918
SfcI C'TryA_G
Cuts at: 0 336 779 2018 2570 3031 3438 3741
Size: 336 443 1239 552 461 407 303
Fragments arranged by size:
1239 552 461 443 407 336 303
SmlI C'TyrA_G
Cuts at: 0 520 1165 2410 2609 3388 3741
Size: 520 645 1245 199 779 353
Fragments arranged by size:
1245 779 645 520 353 199
SpeI A'CTAG_T
Cuts at: 0 799 3741
Size: 799 2942
SspI AAT'ATT
Cuts at: 0 1287 3741
Size: 1287 2454
StuI AGG'CCT
Cuts at: 0 556 2075 3741
Size: 556 1519 1666
Fragments arranged by size:
1666 1519 556
StyI C'CwwG_G
Cuts at: 0 557 986 1262 1732 3741
Size: 557 429 276 470 2009
Fragments arranged by size:
2009 557 470 429 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 221 1738 2111 2931 3347 3496 3741
Size: 221 1517 373 820 416 149 245
Fragments arranged by size:
1517 820 416 373 245 221 149
TatI w'GTAC_w
Cuts at: 0 1821 3107 3615 3741
Size: 1821 1286 508 126
Fragments arranged by size:
1821 1286 508 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1670 1702 1780 1812 3741
Size: 1670 32 78 32 1929
Fragments arranged by size:
1929 1670 78 32 32
XbaI T'CTAG_A
Cuts at: 0 135 351 1470 1644 3741
Size: 135 216 1119 174 2097
Fragments arranged by size:
2097 1119 216 174 135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2824 3741
Size: 2824 917
XmnI GAAnn'nnTTC
Cuts at: 0 347 2925 3083 3741
Size: 347 2578 158 658
Fragments arranged by size:
2578 658 347 158
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrDI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII HpaI
KpnI MfeI MmeI MscI MslI MspA1I NcoI NheI
PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII
SanDI SbfI ScaI SfcI SmlI SpeI SspI StuI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI ApaLI
AscI AsiSI AvrII BbeI BcgI BclI BfrBI BsiEI
BsiWI BsmBI BspEI BspMI BsrFI BsrGI BssHII BstAPI
BstBI BstZ17I ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoRI FseI FspI FspAI HaeII HindIII KasI
MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI Tth111I XhoI XmaI ZraI