Restriction map for
pF1KB0026
(Linear) (Six-base) MAPSORT of: KIBB0026.seq Check: 9268 from: 1 to: 3174
KIBB0026.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 867 1888 3174
Size: 867 1021 1286
Fragments arranged by size:
1286 1021 867
AatII G_ACGT'C
Cuts at: 0 2663 3174
Size: 2663 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 946 1669 1819 2459 3174
Size: 922 24 723 150 640 715
Fragments arranged by size:
922 723 715 640 150 24
AfeI AGC'GCT
Cuts at: 0 767 3174
Size: 767 2407
AflII C'TTAA_G
Cuts at: 0 718 3174
Size: 718 2456
AleI CACnn'nnGTG
Cuts at: 0 1306 2329 2915 3174
Size: 1306 1023 586 259
Fragments arranged by size:
1306 1023 586 259
AlwNI CAG_nnn'CTG
Cuts at: 0 180 1212 1609 2139 2196 2277 2370 2718
Size: 180 1032 397 530 57 81 93 348
Cuts at: 2718 3174
Size: 456
Fragments arranged by size:
1032 530 456 397 348 180 93 81
57
ApaI G_GGCC'C
Cuts at: 0 1842 3174
Size: 1842 1332
ApaLI G'TGCA_C
Cuts at: 0 730 2281 3174
Size: 730 1551 893
Fragments arranged by size:
1551 893 730
ApoI r'AATT_y
Cuts at: 0 268 1625 2251 3174
Size: 268 1357 626 923
Fragments arranged by size:
1357 923 626 268
AvaI C'yCGr_G
Cuts at: 0 1267 1791 1905 3174
Size: 1267 524 114 1269
Fragments arranged by size:
1269 1267 524 114
BamHI G'GATC_C
Cuts at: 0 1703 3174
Size: 1703 1471
BanI G'GyrC_C
Cuts at: 0 1472 1545 1900 2010 2779 3174
Size: 1472 73 355 110 769 395
Fragments arranged by size:
1472 769 395 355 110 73
BanII G_rGCy'C
Cuts at: 0 775 1091 1427 1522 1574 1842 1912 2834
Size: 775 316 336 95 52 268 70 922
Cuts at: 2834 3021 3174
Size: 187 153
Fragments arranged by size:
922 775 336 316 268 187 153 95
70 52
BbeI G_GCGC'C
Cuts at: 0 1476 2014 3174
Size: 1476 538 1160
Fragments arranged by size:
1476 1160 538
BbsI GAAGACnn'nnnn_
Cuts at: 0 1453 2754 3174
Size: 1453 1301 420
Fragments arranged by size:
1453 1301 420
BbvCI CC'TCA_GC
Cuts at: 0 1155 2158 2470 3174
Size: 1155 1003 312 704
Fragments arranged by size:
1155 1003 704 312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1330 1364 1886 1920 2559 2593 3174
Size: 1330 34 522 34 639 34 581
Fragments arranged by size:
1330 639 581 522 34 34 34
BclI T'GATC_A
Cuts at: 0 134 182 1463 3174
Size: 134 48 1281 1711
Fragments arranged by size:
1711 1281 134 48
BglI GCCn_nnn'nGGC
Cuts at: 0 1891 2777 3174
Size: 1891 886 397
Fragments arranged by size:
1891 886 397
BglII A'GATC_T
Cuts at: 0 1163 3174
Size: 1163 2011
Bme1580I G_kGCm'C
Cuts at: 0 734 988 1842 2034 2285 2782 3157 3174
Size: 734 254 854 192 251 497 375 17
Fragments arranged by size:
854 734 497 375 254 251 192 17
BmgBI CAC'GTC
Cuts at: 0 1644 3174
Size: 1644 1530
BmrI ACTGGGnnnn_n'
Cuts at: 0 1393 3174
Size: 1393 1781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2817 3174
Size: 2817 357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1105 1555 1846 2026 2056 2713 2846 2898
Size: 1105 450 291 180 30 657 133 52
Cuts at: 2898 3043 3174
Size: 145 131
Fragments arranged by size:
1105 657 450 291 180 145 133 131
52 30
Bpu10I CC'TnA_GC
Cuts at: 0 1155 1516 2158 2470 3174
Size: 1155 361 642 312 704
Fragments arranged by size:
1155 704 642 361 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 372 1054 3174
Size: 44 328 682 2120
Fragments arranged by size:
2120 682 328 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 792 2093 3174
Size: 792 1301 1081
Fragments arranged by size:
1301 1081 792
BsaHI Gr'CG_yC
Cuts at: 0 615 1219 1473 2011 2660 2935 3174
Size: 615 604 254 538 649 275 239
Fragments arranged by size:
649 615 604 538 275 254 239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 1045 1075 1798 1828 2146 2176
Size: 8 30 1007 30 723 30 318 30
Cuts at: 2176 2842 2872 3174
Size: 666 30 302
Fragments arranged by size:
1007 723 666 318 302 30 30 30
30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 304 735 1435 1447 1575 2140 2365
Size: 79 225 431 700 12 128 565 225
Cuts at: 2365 3050 3076 3174
Size: 685 26 98
Fragments arranged by size:
700 685 565 431 225 225 128 98
79 26 12
BseYI C'CCAG_C
Cuts at: 0 401 470 768 1686 2775 2783 3174
Size: 401 69 298 918 1089 8 391
Fragments arranged by size:
1089 918 401 391 298 69 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 2563 3174
Size: 892 1671 611
Fragments arranged by size:
1671 892 611
BsiEI CG_ry'CG
Cuts at: 0 2429 3174
Size: 2429 745
BsiHKAI G_wGCw'C
Cuts at: 0 164 734 1522 2285 2684 2834 3021 3174
Size: 164 570 788 763 399 150 187 153
Fragments arranged by size:
788 763 570 399 187 164 153 150
BsmI GAATG_Cn'
Cuts at: 0 235 3174
Size: 235 2939
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1639 3174
Size: 1639 1535
Bsp1286I G_dGCh'C
Cuts at: 0 164 734 775 988 1091 1427 1522 1574
Size: 164 570 41 213 103 336 95 52
Cuts at: 1574 1842 1912 2034 2285 2684 2782 2834 3021
Size: 268 70 122 251 399 98 52 187
Cuts at: 3021 3157 3174
Size: 136 17
Fragments arranged by size:
570 399 336 268 251 213 187 164
136 122 103 98 95 70 52 52
41 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 860 867 1888 2147 2276 3155 3174
Size: 860 7 1021 259 129 879 19
Fragments arranged by size:
1021 879 860 259 129 19 7
BsrBI CCG'CTC
Cuts at: 0 8 3174
Size: 8 3166
BsrDI GCAATG_nn'
Cuts at: 0 329 3174
Size: 329 2845
BsrGI T'GTAC_A
Cuts at: 0 2432 3174
Size: 2432 742
BssSI C'ACGA_G
Cuts at: 0 650 2568 3174
Size: 650 1918 606
Fragments arranged by size:
1918 650 606
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1212 2054 3174
Size: 1212 842 1120
Fragments arranged by size:
1212 1120 842
BstXI CCAn_nnnn'nTGG
Cuts at: 0 207 768 972 1503 1686 1981 2917 2922
Size: 207 561 204 531 183 295 936 5
Cuts at: 2922 3174
Size: 252
Fragments arranged by size:
936 561 531 295 252 207 204 183
5
BstYI r'GATC_y
Cuts at: 0 111 1163 1703 1760 1985 2634 3174
Size: 111 1052 540 57 225 649 540
Fragments arranged by size:
1052 649 540 540 225 111 57
Bsu36I CC'TnA_GG
Cuts at: 0 992 3174
Size: 992 2182
BtgI C'CryG_G
Cuts at: 0 488 1307 3174
Size: 488 819 1867
Fragments arranged by size:
1867 819 488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 216 1064 2430 3174
Size: 216 848 1366 744
Fragments arranged by size:
1366 848 744 216
BtsI GCAGTG_nn'
Cuts at: 0 18 430 891 2371 3174
Size: 18 412 461 1480 803
Fragments arranged by size:
1480 803 461 412 18
ClaI AT'CG_AT
Cuts at: 0 80 3174
Size: 80 3094
DraIII CAC_nnn'GTG
Cuts at: 0 3167 3174
Size: 3167 7
EaeI y'GGCC_r
Cuts at: 0 866 1170 1358 1766 1892 2426 3174
Size: 866 304 188 408 126 534 748
Fragments arranged by size:
866 748 534 408 304 188 126
EagI C'GGCC_G
Cuts at: 0 2426 3174
Size: 2426 748
EarI CTCTTCn'nnn_
Cuts at: 0 683 1535 3068 3174
Size: 683 852 1533 106
Fragments arranged by size:
1533 852 683 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1819 1930 3174
Size: 1819 111 1244
Fragments arranged by size:
1819 1244 111
EcoICRI GAG'CTC
Cuts at: 0 1520 2832 3019 3174
Size: 1520 1312 187 155
Fragments arranged by size:
1520 1312 187 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 946 1105 1555 1669 1819 1846 2026
Size: 922 24 159 450 114 150 27 180
Cuts at: 2026 2056 2459 2713 2846 2898 3043 3174
Size: 30 403 254 133 52 145 131
Fragments arranged by size:
922 450 403 254 180 159 150 145
133 131 114 52 30 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 99 1099 1267 3174
Size: 99 1000 168 1907
Fragments arranged by size:
1907 1000 168 99
EcoO109I rG'GnC_Cy
Cuts at: 0 564 1142 1809 2193 2715 2946 3174
Size: 564 578 667 384 522 231 228
Fragments arranged by size:
667 578 564 522 384 231 228
EcoRV GAT'ATC
Cuts at: 0 630 3174
Size: 630 2544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 720 2778 3174
Size: 720 2058 396
Fragments arranged by size:
2058 720 396
HaeII r_GCGC'y
Cuts at: 0 133 769 1476 1610 1694 2014 2118 2902
Size: 133 636 707 134 84 320 104 784
Cuts at: 2902 3174
Size: 272
Fragments arranged by size:
784 707 636 320 272 134 133 104
84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 466 498 1045 1077 1798 1830
Size: 8 32 426 32 547 32 721 32
Cuts at: 1830 2090 2122 2146 2178 2863 2895 2983 3015
Size: 260 32 24 32 685 32 88 32
Cuts at: 3015 3174
Size: 159
Fragments arranged by size:
721 685 547 426 260 159 88 32
32 32 32 32 32 32 32 24
8
KasI G'GCGC_C
Cuts at: 0 1472 2010 3174
Size: 1472 538 1164
Fragments arranged by size:
1472 1164 538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 3174
Size: 13 3161
MscI TGG'CCA
Cuts at: 0 868 1360 1768 3174
Size: 868 492 408 1406
Fragments arranged by size:
1406 868 492 408
MslI CAynn'nnrTG
Cuts at: 0 808 1252 1306 1366 1501 2329 2677 2749
Size: 808 444 54 60 135 828 348 72
Cuts at: 2749 2915 2960 3174
Size: 166 45 214
Fragments arranged by size:
828 808 444 348 214 166 135 72
60 54 45
MspA1I CmG'CkG
Cuts at: 0 714 1195 1375 1736 2232 2817 3174
Size: 714 481 180 361 496 585 357
Fragments arranged by size:
714 585 496 481 361 357 180
NarI GG'CG_CC
Cuts at: 0 1473 2011 3174
Size: 1473 538 1163
Fragments arranged by size:
1473 1163 538
NcoI C'CATG_G
Cuts at: 0 488 3174
Size: 488 2686
NspI r_CATG'y
Cuts at: 0 299 348 847 1017 2088 2146 2217 2965
Size: 299 49 499 170 1071 58 71 748
Cuts at: 2965 3174
Size: 209
Fragments arranged by size:
1071 748 499 299 209 170 71 58
49
PasI CC'CwG_GG
Cuts at: 0 1485 3174
Size: 1485 1689
PflMI CCAn_nnn'nTGG
Cuts at: 0 2421 3174
Size: 2421 753
PfoI T'CCnGG_A
Cuts at: 0 173 218 1823 2630 3174
Size: 173 45 1605 807 544
Fragments arranged by size:
1605 807 544 173 45
PpuMI rG'GwC_Cy
Cuts at: 0 1142 1809 2946 3174
Size: 1142 667 1137 228
Fragments arranged by size:
1142 1137 667 228
PshAI GACnn'nnGTC
Cuts at: 0 2563 2951 3174
Size: 2563 388 223
Fragments arranged by size:
2563 388 223
PspOMI G'GGCC_C
Cuts at: 0 1838 3174
Size: 1838 1336
PstI C_TGCA'G
Cuts at: 0 425 1208 1735 1961 2273 3174
Size: 425 783 527 226 312 901
Fragments arranged by size:
901 783 527 425 312 226
PvuII CAG'CTG
Cuts at: 0 714 1195 1375 2232 3174
Size: 714 481 180 857 942
Fragments arranged by size:
942 857 714 481 180
SacI G_AGCT'C
Cuts at: 0 1522 2834 3021 3174
Size: 1522 1312 187 153
Fragments arranged by size:
1522 1312 187 153
SbfI CC_TGCA'GG
Cuts at: 0 1961 3174
Size: 1961 1213
ScaI AGT'ACT
Cuts at: 0 2053 3174
Size: 2053 1121
SexAI A'CCwGG_T
Cuts at: 0 1389 3174
Size: 1389 1785
SfcI C'TryA_G
Cuts at: 0 421 457 1204 1731 1957 2269 3174
Size: 421 36 747 527 226 312 905
Fragments arranged by size:
905 747 527 421 312 226 36
SfoI GGC'GCC
Cuts at: 0 1474 2012 3174
Size: 1474 538 1162
Fragments arranged by size:
1474 1162 538
SmlI C'TyrA_G
Cuts at: 0 59 351 718 1069 3174
Size: 59 292 367 351 2105
Fragments arranged by size:
2105 367 351 292 59
SphI G_CATG'C
Cuts at: 0 299 847 3174
Size: 299 548 2327
Fragments arranged by size:
2327 548 299
StuI AGG'CCT
Cuts at: 0 466 829 1153 3174
Size: 466 363 324 2021
Fragments arranged by size:
2021 466 363 324
StyI C'CwwG_G
Cuts at: 0 488 704 941 1427 1994 2546 3174
Size: 488 216 237 486 567 552 628
Fragments arranged by size:
628 567 552 488 486 237 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 2797 2972 3174
Size: 556 2241 175 202
Fragments arranged by size:
2241 556 202 175
TatI w'GTAC_w
Cuts at: 0 671 1877 2051 2432 3174
Size: 671 1206 174 381 742
Fragments arranged by size:
1206 742 671 381 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 922 954 3174
Size: 922 32 2220
Fragments arranged by size:
2220 922 32
Tth111I GACn'n_nGTC
Cuts at: 0 22 2596 3174
Size: 22 2574 578
Fragments arranged by size:
2574 578 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 972 1503 1530 2001 3174
Size: 555 417 531 27 471 1173
Fragments arranged by size:
1173 555 531 471 417 27
XmnI GAAnn'nnTTC
Cuts at: 0 1627 3174
Size: 1627 1547
ZraI GAC'GTC
Cuts at: 0 2661 3174
Size: 2661 513
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SbfI ScaI SexAI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflIII AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BciVI BfrBI BlpI
BmtI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrFI
BssHII BstBI BstEII BstZ17I CspCI DraI DrdI EcoRI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI