Restriction map for
pF1KSDB0030
(Linear) (Six-base) MAPSORT of: KIBB0030.seq Check: 9029 from: 1 to: 4818
KIBB0030.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3567 4818
Size: 3567 1251
AatII G_ACGT'C
Cuts at: 0 2935 4818
Size: 2935 1883
AccI GT'mk_AC
Cuts at: 0 260 3874 3952 4291 4818
Size: 260 3614 78 339 527
Fragments arranged by size:
3614 527 339 260 78
Acc65I G'GTAC_C
Cuts at: 0 3408 4818
Size: 3408 1410
AclI AA'CG_TT
Cuts at: 0 1851 4818
Size: 1851 2967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1142 1343 2680 2764 3561 3739 4265
Size: 767 375 201 1337 84 797 178 526
Cuts at: 4265 4393 4818
Size: 128 425
Fragments arranged by size:
1337 797 767 526 425 375 201 178
128 84
AflII C'TTAA_G
Cuts at: 0 1263 2791 4818
Size: 1263 1528 2027
Fragments arranged by size:
2027 1528 1263
AflIII A'CryG_T
Cuts at: 0 2471 3435 4025 4448 4818
Size: 2471 964 590 423 370
Fragments arranged by size:
2471 964 590 423 370
AgeI A'CCGG_T
Cuts at: 0 722 1020 4818
Size: 722 298 3798
Fragments arranged by size:
3798 722 298
AleI CACnn'nnGTG
Cuts at: 0 490 1537 4818
Size: 490 1047 3281
Fragments arranged by size:
3281 1047 490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1723 3007 3828 4818
Size: 1723 1284 821 990
Fragments arranged by size:
1723 1284 990 821
AlwNI CAG_nnn'CTG
Cuts at: 0 232 1033 1549 1693 2355 2527 3355 3825
Size: 232 801 516 144 662 172 828 470
Cuts at: 3825 3921 4818
Size: 96 897
Fragments arranged by size:
897 828 801 662 516 470 232 172
144 96
ApaI G_GGCC'C
Cuts at: 0 189 621 2069 4818
Size: 189 432 1448 2749
Fragments arranged by size:
2749 1448 432 189
ApoI r'AATT_y
Cuts at: 0 430 563 1091 1512 1760 4234 4818
Size: 430 133 528 421 248 2474 584
Fragments arranged by size:
2474 584 528 430 421 248 133
AvaI C'yCGr_G
Cuts at: 0 72 195 212 1786 2681 4818
Size: 72 123 17 1574 895 2137
Fragments arranged by size:
2137 1574 895 123 72 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 361 394 4818
Size: 361 33 4424
Fragments arranged by size:
4424 361 33
BamHI G'GATC_C
Cuts at: 0 271 1977 4818
Size: 271 1706 2841
Fragments arranged by size:
2841 1706 271
BanI G'GyrC_C
Cuts at: 0 448 1317 2991 3408 4077 4818
Size: 448 869 1674 417 669 741
Fragments arranged by size:
1674 869 741 669 448 417
BanII G_rGCy'C
Cuts at: 0 189 399 621 1242 2069 2447 3715 4464
Size: 189 210 222 621 827 378 1268 749
Cuts at: 4464 4818
Size: 354
Fragments arranged by size:
1268 827 749 621 378 354 222 210
189
BbeI G_GCGC'C
Cuts at: 0 452 1321 4818
Size: 452 869 3497
Fragments arranged by size:
3497 869 452
BbsI GAAGACnn'nnnn_
Cuts at: 0 1760 2935 4818
Size: 1760 1175 1883
Fragments arranged by size:
1883 1760 1175
BbvCI CC'TCA_GC
Cuts at: 0 3998 4818
Size: 3998 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4533 4567 4818
Size: 4533 34 251
Fragments arranged by size:
4533 251 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4452 4818
Size: 4452 366
BfrBI ATG'CAT
Cuts at: 0 2769 4818
Size: 2769 2049
BglI GCCn_nnn'nGGC
Cuts at: 0 2103 3627 4606 4818
Size: 2103 1524 979 212
Fragments arranged by size:
2103 1524 979 212
BglII A'GATC_T
Cuts at: 0 299 1125 4818
Size: 299 826 3693
Fragments arranged by size:
3693 826 299
BlpI GC'TnA_GC
Cuts at: 0 4222 4730 4818
Size: 4222 508 88
Fragments arranged by size:
4222 508 88
Bme1580I G_kGCm'C
Cuts at: 0 189 621 1199 1712 2069 2504 4082 4332
Size: 189 432 578 513 357 435 1578 250
Cuts at: 4332 4818
Size: 486
Fragments arranged by size:
1578 578 513 486 435 432 357 250
189
BmgBI CAC'GTC
Cuts at: 0 1717 1935 2964 3436 4680 4818
Size: 1717 218 1029 472 1244 138
Fragments arranged by size:
1717 1244 1029 472 218 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 499 823 950 1264 3700 4818
Size: 499 324 127 314 2436 1118
Fragments arranged by size:
2436 1118 499 324 314 127
BmtI G_CTAG'C
Cuts at: 0 3647 4818
Size: 3647 1171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3846 4818
Size: 3846 972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 947 1039 1328 3110 3302 3503 3901
Size: 685 262 92 289 1782 192 201 398
Cuts at: 3901 4171 4381 4588 4818
Size: 270 210 207 230
Fragments arranged by size:
1782 685 398 289 270 262 230 210
207 201 192 92
Bpu10I CC'TnA_GC
Cuts at: 0 1376 2053 3321 3998 4818
Size: 1376 677 1268 677 820
Fragments arranged by size:
1376 1268 820 677 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1520 2836 3477 4818
Size: 656 864 1316 641 1341
Fragments arranged by size:
1341 1316 864 656 641
BsaI GGTCTCn'nnnn_
Cuts at: 0 164 1598 1729 3479 3488 4113 4818
Size: 164 1434 131 1750 9 625 705
Fragments arranged by size:
1750 1434 705 625 164 131 9
BsaAI yAC'GTr
Cuts at: 0 4026 4818
Size: 4026 792
BsaBI GATnn'nnATC
Cuts at: 0 2255 4818
Size: 2255 2563
BsaHI Gr'CG_yC
Cuts at: 0 449 688 1318 2655 2932 4818
Size: 449 239 630 1337 277 1886
Fragments arranged by size:
1886 1337 630 449 277 239
BsaWI w'CCGG_w
Cuts at: 0 722 1020 4818
Size: 722 298 3798
Fragments arranged by size:
3798 722 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 595 625 4818
Size: 298 30 267 30 4193
Fragments arranged by size:
4193 298 267 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 965 1154 1300 1396 1472 1575 1855
Size: 616 349 189 146 96 76 103 280
Cuts at: 1855 3462 3506 3607 3751 3754 4318 4357 4813
Size: 1607 44 101 144 3 564 39 456
Cuts at: 4813 4816 4818
Size: 3 2
Fragments arranged by size:
1607 616 564 456 349 280 189 146
144 103 101 96 76 44 39 3
3 2
BseYI C'CCAG_C
Cuts at: 0 188 246 309 791 1170 1371 2227 3296
Size: 188 58 63 482 379 201 856 1069
Cuts at: 3296 3363 3617 3940 4818
Size: 67 254 323 878
Fragments arranged by size:
1069 878 856 482 379 323 254 201
188 67 63 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 238 245 406 493 3100 3463 3838
Size: 224 14 7 161 87 2607 363 375
Cuts at: 3838 3907 4818
Size: 69 911
Fragments arranged by size:
2607 911 375 363 224 161 87 69
14 7
BsiEI CG_ry'CG
Cuts at: 0 1860 4818
Size: 1860 2958
BsiHKAI G_wGCw'C
Cuts at: 0 3071 4818
Size: 3071 1747
BsmI GAATG_Cn'
Cuts at: 0 4264 4818
Size: 4264 554
BsmBI CGTCTCn'nnnn_
Cuts at: 0 679 1557 4818
Size: 679 878 3261
Fragments arranged by size:
3261 878 679
Bsp1286I G_dGCh'C
Cuts at: 0 189 399 621 1199 1242 1712 2069 2447
Size: 189 210 222 578 43 470 357 378
Cuts at: 2447 2504 3071 3715 4082 4332 4464 4818
Size: 57 567 644 367 250 132 354
Fragments arranged by size:
644 578 567 470 378 367 357 354
250 222 210 189 132 57 43
BspHI T'CATG_A
Cuts at: 0 1095 4466 4818
Size: 1095 3371 352
Fragments arranged by size:
3371 1095 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3567 4649 4818
Size: 3567 1082 169
Fragments arranged by size:
3567 1082 169
BsrBI CCG'CTC
Cuts at: 0 3507 3597 4818
Size: 3507 90 1221
Fragments arranged by size:
3507 1221 90
BsrDI GCAATG_nn'
Cuts at: 0 740 1199 2165 3893 4818
Size: 740 459 966 1728 925
Fragments arranged by size:
1728 966 925 740 459
BsrFI r'CCGG_y
Cuts at: 0 87 722 749 1020 2106 3536 4818
Size: 87 635 27 271 1086 1430 1282
Fragments arranged by size:
1430 1282 1086 635 271 87 27
BssSI C'ACGA_G
Cuts at: 0 3899 4818
Size: 3899 919
BstAPI GCAn_nnn'nTGC
Cuts at: 0 232 742 2964 3921 3930 4818
Size: 232 510 2222 957 9 888
Fragments arranged by size:
2222 957 888 510 232 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1071 1539 4258 4818
Size: 492 579 468 2719 560
Fragments arranged by size:
2719 579 560 492 468
BstYI r'GATC_y
Cuts at: 0 271 299 570 1125 1977 2970 3032 4154
Size: 271 28 271 555 852 993 62 1122
Cuts at: 4154 4818
Size: 664
Fragments arranged by size:
1122 993 852 664 555 271 271 62
28
BstZ17I GTA'TAC
Cuts at: 0 4292 4818
Size: 4292 526
Bsu36I CC'TnA_GG
Cuts at: 0 1220 1721 3623 4818
Size: 1220 501 1902 1195
Fragments arranged by size:
1902 1220 1195 501
BtgI C'CryG_G
Cuts at: 0 2061 2100 3144 3528 3666 4082 4818
Size: 2061 39 1044 384 138 416 736
Fragments arranged by size:
2061 1044 736 416 384 138 39
BtsI GCAGTG_nn'
Cuts at: 0 207 623 1707 1963 3108 3894 4532 4818
Size: 207 416 1084 256 1145 786 638 286
Fragments arranged by size:
1145 1084 786 638 416 286 256 207
ClaI AT'CG_AT
Cuts at: 0 2556 3254 4818
Size: 2556 698 1564
Fragments arranged by size:
2556 1564 698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 342 377 4818
Size: 342 35 4441
Fragments arranged by size:
4441 342 35
DraIII CAC_nnn'GTG
Cuts at: 0 1086 4818
Size: 1086 3732
DrdI GACnn_nn'nnGTC
Cuts at: 0 2779 4818
Size: 2779 2039
EaeI y'GGCC_r
Cuts at: 0 712 751 1969 2040 2378 3092 4320 4818
Size: 712 39 1218 71 338 714 1228 498
Fragments arranged by size:
1228 1218 714 712 498 338 71 39
EarI CTCTTCn'nnn_
Cuts at: 0 1400 2660 2669 2801 3850 4818
Size: 1400 1260 9 132 1049 968
Fragments arranged by size:
1400 1260 1049 968 132 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 767 947 1039 1142 1328 1343 2680
Size: 685 82 180 92 103 186 15 1337
Cuts at: 2680 2764 3110 3302 3503 3561 3739 3901 4171
Size: 84 346 192 201 58 178 162 270
Cuts at: 4171 4265 4381 4393 4588 4818
Size: 94 116 12 195 230
Fragments arranged by size:
1337 685 346 270 230 201 195 192
186 180 178 162 116 103 94 92
84 82 58 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1667 3652 4300 4818
Size: 1667 1985 648 518
Fragments arranged by size:
1985 1667 648 518
EcoO109I rG'GnC_Cy
Cuts at: 0 25 323 437 617 2066 2119 2140 2295
Size: 25 298 114 180 1449 53 21 155
Cuts at: 2295 3013 3130 4183 4608 4722 4818
Size: 718 117 1053 425 114 96
Fragments arranged by size:
1449 1053 718 425 298 180 155 117
114 114 96 53 25 21
EcoRI G'AATT_C
Cuts at: 0 563 4818
Size: 563 4255
EcoRV GAT'ATC
Cuts at: 0 594 4818
Size: 594 4224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1027 2830 3591 4237 4818
Size: 1027 1803 761 646 581
Fragments arranged by size:
1803 1027 761 646 581
FspI TGC'GCA
Cuts at: 0 3634 4818
Size: 3634 1184
HaeII r_GCGC'y
Cuts at: 0 88 452 1321 2130 2494 2703 4594 4818
Size: 88 364 869 809 364 209 1891 224
Fragments arranged by size:
1891 869 809 364 364 224 209 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 709 741 1129 1161 2794 2826
Size: 595 32 82 32 388 32 1633 32
Cuts at: 2826 3056 3088 3331 3363 3739 3771 4789 4818
Size: 230 32 243 32 376 32 1018 29
Fragments arranged by size:
1633 1018 595 388 376 243 230 82
32 32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 2753 4818
Size: 2753 2065
HpaI GTT'AAC
Cuts at: 0 2753 4818
Size: 2753 2065
KasI G'GCGC_C
Cuts at: 0 448 1317 4818
Size: 448 869 3501
Fragments arranged by size:
3501 869 448
KpnI G_GTAC'C
Cuts at: 0 3412 4818
Size: 3412 1406
MfeI C'AATT_G
Cuts at: 0 2985 4818
Size: 2985 1833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1231 1581 4289 4469 4818
Size: 835 396 350 2708 180 349
Fragments arranged by size:
2708 835 396 350 349 180
MscI TGG'CCA
Cuts at: 0 2380 3094 4322 4818
Size: 2380 714 1228 496
Fragments arranged by size:
2380 1228 714 496
MslI CAynn'nnrTG
Cuts at: 0 490 1537 1928 2774 3076 3788 4818
Size: 490 1047 391 846 302 712 1030
Fragments arranged by size:
1047 1030 846 712 490 391 302
MspA1I CmG'CkG
Cuts at: 0 250 1255 2355 2376 2480 3314 3918 3930
Size: 250 1005 1100 21 104 834 604 12
Cuts at: 3930 4359 4395 4729 4818
Size: 429 36 334 89
Fragments arranged by size:
1100 1005 834 604 429 334 250 104
89 36 21 12
NaeI GCC'GGC
Cuts at: 0 2108 4818
Size: 2108 2710
NarI GG'CG_CC
Cuts at: 0 449 1318 4818
Size: 449 869 3500
Fragments arranged by size:
3500 869 449
NcoI C'CATG_G
Cuts at: 0 4082 4818
Size: 4082 736
NdeI CA'TA_TG
Cuts at: 0 3908 4818
Size: 3908 910
NgoMIV G'CCGG_C
Cuts at: 0 2106 4818
Size: 2106 2712
NheI G'CTAG_C
Cuts at: 0 3643 4818
Size: 3643 1175
NsiI A_TGCA'T
Cuts at: 0 2771 4818
Size: 2771 2047
NspI r_CATG'y
Cuts at: 0 2475 4818
Size: 2475 2343
PasI CC'CwG_GG
Cuts at: 0 318 4296 4818
Size: 318 3978 522
Fragments arranged by size:
3978 522 318
PciI A'CATG_T
Cuts at: 0 2471 4818
Size: 2471 2347
PflMI CCAn_nnn'nTGG
Cuts at: 0 2077 4818
Size: 2077 2741
PmlI CAC'GTG
Cuts at: 0 4026 4818
Size: 4026 792
PpuMI rG'GwC_Cy
Cuts at: 0 437 2119 2140 2295 3013 4183 4722 4818
Size: 437 1682 21 155 718 1170 539 96
Fragments arranged by size:
1682 1170 718 539 437 155 96 21
PshAI GACnn'nnGTC
Cuts at: 0 3110 4474 4818
Size: 3110 1364 344
Fragments arranged by size:
3110 1364 344
PspOMI G'GGCC_C
Cuts at: 0 185 617 2065 4818
Size: 185 432 1448 2753
Fragments arranged by size:
2753 1448 432 185
PstI C_TGCA'G
Cuts at: 0 167 1671 2960 4067 4556 4818
Size: 167 1504 1289 1107 489 262
Fragments arranged by size:
1504 1289 1107 489 262 167
PvuII CAG'CTG
Cuts at: 0 250 1255 2355 2376 3918 3930 4359 4818
Size: 250 1005 1100 21 1542 12 429 459
Fragments arranged by size:
1542 1100 1005 459 429 250 21 12
SanDI GG'GwC_CC
Cuts at: 0 2119 2140 3013 4818
Size: 2119 21 873 1805
Fragments arranged by size:
2119 1805 873 21
SapI GCTCTTCn'nnn_
Cuts at: 0 2660 3850 4818
Size: 2660 1190 968
Fragments arranged by size:
2660 1190 968
SbfI CC_TGCA'GG
Cuts at: 0 1671 4818
Size: 1671 3147
ScaI AGT'ACT
Cuts at: 0 3375 3964 4818
Size: 3375 589 854
Fragments arranged by size:
3375 854 589
SfcI C'TryA_G
Cuts at: 0 163 425 908 1667 2956 4063 4552 4585
Size: 163 262 483 759 1289 1107 489 33
Cuts at: 4585 4701 4818
Size: 116 117
Fragments arranged by size:
1289 1107 759 489 483 262 163 117
116 33
SfoI GGC'GCC
Cuts at: 0 450 1319 4818
Size: 450 869 3499
Fragments arranged by size:
3499 869 450
SgrAI Cr'CCGG_yG
Cuts at: 0 87 4818
Size: 87 4731
SmaI CCC'GGG
Cuts at: 0 74 1788 4818
Size: 74 1714 3030
Fragments arranged by size:
3030 1714 74
SmlI C'TyrA_G
Cuts at: 0 671 1263 1535 2791 2815 3456 4818
Size: 671 592 272 1256 24 641 1362
Fragments arranged by size:
1362 1256 671 641 592 272 24
SpeI A'CTAG_T
Cuts at: 0 3370 4818
Size: 3370 1448
StyI C'CwwG_G
Cuts at: 0 465 579 1295 2037 2071 3095 3758 3969
Size: 465 114 716 742 34 1024 663 211
Cuts at: 3969 4082 4818
Size: 113 736
Fragments arranged by size:
1024 742 736 716 663 465 211 114
113 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 651 2200 2979 3503 4071 4818
Size: 651 1549 779 524 568 747
Fragments arranged by size:
1549 779 747 651 568 524
TatI w'GTAC_w
Cuts at: 0 3373 3962 4818
Size: 3373 589 856
Fragments arranged by size:
3373 856 589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2454 2880 4818
Size: 2454 426 1938
Fragments arranged by size:
2454 1938 426
XmaI C'CCGG_G
Cuts at: 0 72 1786 4818
Size: 72 1714 3032
Fragments arranged by size:
3032 1714 72
XmnI GAAnn'nnTTC
Cuts at: 0 3385 4818
Size: 3385 1433
ZraI GAC'GTC
Cuts at: 0 2933 4818
Size: 2933 1885
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PflMI PmlI PpuMI PshAI PspOMI PstI PvuII
SanDI SapI SbfI ScaI SfcI SfoI SgrAI SmaI
SmlI SpeI StyI TaqII TatI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BclI BsiWI BspEI BsrGI BssHII BstBI BstEII BtgZI
DraI EagI EciI EcoICRI FseI FspAI HindIII MluI
NotI NruI PacI PfoI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SexAI SfiI
SgfI SnaBI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI