Restriction map for
pF1KSDB0035
(Linear) (Six-base) MAPSORT of: KIBB0035.seq Check: 2797 from: 1 to: 1407
KIBB0035.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
Acc65I G'GTAC_C
Cuts at: 0 236 639 1032 1407
Size: 236 403 393 375
Fragments arranged by size:
403 393 375 236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1407
Size: 1163 244
AleI CACnn'nnGTG
Cuts at: 0 246 1407
Size: 246 1161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 1407
Size: 426 32 949
Fragments arranged by size:
949 426 32
AlwNI CAG_nnn'CTG
Cuts at: 0 805 1017 1063 1087 1407
Size: 805 212 46 24 320
Fragments arranged by size:
805 320 212 46 24
ApoI r'AATT_y
Cuts at: 0 90 273 416 1250 1279 1306 1407
Size: 90 183 143 834 29 27 101
Fragments arranged by size:
834 183 143 101 90 29 27
AvaI C'yCGr_G
Cuts at: 0 713 1407
Size: 713 694
BanI G'GyrC_C
Cuts at: 0 236 639 757 1032 1147 1407
Size: 236 403 118 275 115 260
Fragments arranged by size:
403 275 260 236 118 115
BanII G_rGCy'C
Cuts at: 0 623 1407
Size: 623 784
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 533 726 1407
Size: 193 340 193 681
Fragments arranged by size:
681 340 193 193
Bme1580I G_kGCm'C
Cuts at: 0 760 1152 1407
Size: 760 392 255
Fragments arranged by size:
760 392 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 770 1407
Size: 770 637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1059 1407
Size: 827 232 348
Fragments arranged by size:
827 348 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1407
Size: 898 509
BsaWI w'CCGG_w
Cuts at: 0 1131 1407
Size: 1131 276
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 889 919 1407
Size: 889 30 488
Fragments arranged by size:
889 488 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 692 713 1407
Size: 692 21 694
Fragments arranged by size:
694 692 21
BsiHKAI G_wGCw'C
Cuts at: 0 623 1407
Size: 623 784
BsmI GAATG_Cn'
Cuts at: 0 30 1407
Size: 30 1377
Bsp1286I G_dGCh'C
Cuts at: 0 623 760 1152 1407
Size: 623 137 392 255
Fragments arranged by size:
623 392 255 137
BsrDI GCAATG_nn'
Cuts at: 0 1200 1407
Size: 1200 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 248 428 1407
Size: 248 180 979
Fragments arranged by size:
979 248 180
BstYI r'GATC_y
Cuts at: 0 434 601 863 1407
Size: 434 167 262 544
Fragments arranged by size:
544 434 262 167
Bsu36I CC'TnA_GG
Cuts at: 0 1028 1342 1407
Size: 1028 314 65
Fragments arranged by size:
1028 314 65
BtsI GCAGTG_nn'
Cuts at: 0 1173 1407
Size: 1173 234
DraIII CAC_nnn'GTG
Cuts at: 0 184 1407
Size: 184 1223
DrdI GACnn_nn'nnGTC
Cuts at: 0 982 1407
Size: 982 425
EaeI y'GGCC_r
Cuts at: 0 1241 1407
Size: 1241 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 876 1407
Size: 876 531
EcoICRI GAG'CTC
Cuts at: 0 621 1407
Size: 621 786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1059 1163 1407
Size: 827 232 104 244
Fragments arranged by size:
827 244 232 104
EcoRI G'AATT_C
Cuts at: 0 273 416 1407
Size: 273 143 991
Fragments arranged by size:
991 273 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 374 1168 1407
Size: 374 794 239
Fragments arranged by size:
794 374 239
HaeII r_GCGC'y
Cuts at: 0 514 1407
Size: 514 893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1407
Size: 1081 32 294
Fragments arranged by size:
1081 294 32
HincII GTy'rAC
Cuts at: 0 381 1407
Size: 381 1026
HindIII A'AGCT_T
Cuts at: 0 1353 1407
Size: 1353 54
KpnI G_GTAC'C
Cuts at: 0 240 643 1036 1407
Size: 240 403 393 371
Fragments arranged by size:
403 393 371 240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 932 1407
Size: 309 623 475
Fragments arranged by size:
623 475 309
MslI CAynn'nnrTG
Cuts at: 0 246 451 1407
Size: 246 205 956
Fragments arranged by size:
956 246 205
MspA1I CmG'CkG
Cuts at: 0 598 768 1407
Size: 598 170 639
Fragments arranged by size:
639 598 170
NspI r_CATG'y
Cuts at: 0 464 1072 1407
Size: 464 608 335
Fragments arranged by size:
608 464 335
PfoI T'CCnGG_A
Cuts at: 0 986 1407
Size: 986 421
PshAI GACnn'nnGTC
Cuts at: 0 197 1229 1407
Size: 197 1032 178
Fragments arranged by size:
1032 197 178
PstI C_TGCA'G
Cuts at: 0 1224 1407
Size: 1224 183
SacI G_AGCT'C
Cuts at: 0 623 1407
Size: 623 784
SfcI C'TryA_G
Cuts at: 0 1220 1407
Size: 1220 187
SmaI CCC'GGG
Cuts at: 0 715 1407
Size: 715 692
SmlI C'TyrA_G
Cuts at: 0 877 1407
Size: 877 530
StuI AGG'CCT
Cuts at: 0 1399 1407
Size: 1399 8
XbaI T'CTAG_A
Cuts at: 0 834 1407
Size: 834 573
XmaI C'CCGG_G
Cuts at: 0 713 1407
Size: 713 694
XmnI GAAnn'nnTTC
Cuts at: 0 668 1407
Size: 668 739
Enzymes that do cut:
Acc65I AcuI AleI AloI AlwNI ApoI AvaI BanI
BanII BbsI Bme1580I BmrI BpmI BpuEI BsaWI BsaXI
BseRI BsiHKAI BsmI Bsp1286I BsrDI BstXI BstYI Bsu36I
BtsI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoRI
FalI HaeII Hin4I HincII HindIII KpnI MmeI MslI
MspA1I NspI PfoI PshAI PstI SacI SfcI SmaI
SmlI StuI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI
DraI EagI EarI EcoNI EcoO109I EcoRV FseI FspI
FspAI HpaI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI TstI Tth111I XcmI XhoI ZraI