(Linear) (Six-base) MAPSORT of: KIBB0037.seq Check: 4957 from: 1 to: 1413
KIBB0037.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 653 1413
Size: 653 760
AccI GT'mk_AC
Cuts at: 0 1289 1413
Size: 1289 124
Acc65I G'GTAC_C
Cuts at: 0 1146 1413
Size: 1146 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 775 1413
Size: 604 171 638
Fragments arranged by size:
638 604 171
AleI CACnn'nnGTG
Cuts at: 0 968 1413
Size: 968 445
ApoI r'AATT_y
Cuts at: 0 529 549 1376 1413
Size: 529 20 827 37
Fragments arranged by size:
827 529 37 20
AvaI C'yCGr_G
Cuts at: 0 734 1413
Size: 734 679
BamHI G'GATC_C
Cuts at: 0 1238 1413
Size: 1238 175
BanI G'GyrC_C
Cuts at: 0 115 1146 1413
Size: 115 1031 267
Fragments arranged by size:
1031 267 115
BanII G_rGCy'C
Cuts at: 0 1222 1413
Size: 1222 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 645 1130 1413
Size: 645 485 283
Fragments arranged by size:
645 485 283
BbvCI CC'TCA_GC
Cuts at: 0 1002 1413
Size: 1002 411
BglII A'GATC_T
Cuts at: 0 809 1413
Size: 809 604
Bme1580I G_kGCm'C
Cuts at: 0 836 1413
Size: 836 577
BmgBI CAC'GTC
Cuts at: 0 660 1039 1413
Size: 660 379 374
Fragments arranged by size:
660 379 374
Bpu10I CC'TnA_GC
Cuts at: 0 1002 1413
Size: 1002 411
BsaI GGTCTCn'nnnn_
Cuts at: 0 406 640 1413
Size: 406 234 773
Fragments arranged by size:
773 406 234
BsaBI GATnn'nnATC
Cuts at: 0 16 269 1013 1413
Size: 16 253 744 400
Fragments arranged by size:
744 400 253 16
BsaHI Gr'CG_yC
Cuts at: 0 650 1413
Size: 650 763
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 955 1413
Size: 955 458
BseYI C'CCAG_C
Cuts at: 0 202 1413
Size: 202 1211
BsmI GAATG_Cn'
Cuts at: 0 497 1413
Size: 497 916
Bsp1286I G_dGCh'C
Cuts at: 0 836 1222 1413
Size: 836 386 191
Fragments arranged by size:
836 386 191
BsrDI GCAATG_nn'
Cuts at: 0 79 746 1413
Size: 79 667 667
Fragments arranged by size:
667 667 79
BsrFI r'CCGG_y
Cuts at: 0 52 1413
Size: 52 1361
BstYI r'GATC_y
Cuts at: 0 809 1238 1272 1413
Size: 809 429 34 141
Fragments arranged by size:
809 429 141 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1000 1413
Size: 1000 413
DraI TTT'AAA
Cuts at: 0 371 1413
Size: 371 1042
EaeI y'GGCC_r
Cuts at: 0 2 50 1413
Size: 2 48 1363
Fragments arranged by size:
1363 48 2
EarI CTCTTCn'nnn_
Cuts at: 0 323 905 1413
Size: 323 582 508
Fragments arranged by size:
582 508 323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 695 1413
Size: 695 718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 775 1413
Size: 604 171 638
Fragments arranged by size:
638 604 171
EcoNI CCTnn'n_nnAGG
Cuts at: 0 152 1413
Size: 152 1261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1012 1413
Size: 1012 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 888 920 1307 1330 1339 1362 1413
Size: 888 32 387 23 9 23 51
Fragments arranged by size:
888 387 51 32 23 23 9
KpnI G_GTAC'C
Cuts at: 0 1150 1413
Size: 1150 263
MfeI C'AATT_G
Cuts at: 0 557 1413
Size: 557 856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 1413
Size: 314 1099
MscI TGG'CCA
Cuts at: 0 4 1413
Size: 4 1409
MslI CAynn'nnrTG
Cuts at: 0 421 968 1413
Size: 421 547 445
Fragments arranged by size:
547 445 421
NaeI GCC'GGC
Cuts at: 0 54 1413
Size: 54 1359
NdeI CA'TA_TG
Cuts at: 0 822 1413
Size: 822 591
NgoMIV G'CCGG_C
Cuts at: 0 52 1413
Size: 52 1361
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 1413
Size: 663 750
ScaI AGT'ACT
Cuts at: 0 796 1413
Size: 796 617
SfcI C'TryA_G
Cuts at: 0 1286 1413
Size: 1286 127
TatI w'GTAC_w
Cuts at: 0 794 1413
Size: 794 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1215 1247 1413
Size: 1215 32 166
Fragments arranged by size:
1215 166 32
XbaI T'CTAG_A
Cuts at: 0 690 1413
Size: 690 723
ZraI GAC'GTC
Cuts at: 0 651 1413
Size: 651 762
Enzymes that do cut:
AatII AccI Acc65I AcuI AleI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BglII Bme1580I BmgBI Bpu10I
BsaI BsaBI BsaHI BseRI BseYI BsmI Bsp1286I BsrDI
BsrFI BstYI BtgZI DraI EaeI EarI EciI Eco57MI
EcoNI FalI Hin4I KpnI MfeI MmeI MscI MslI
NaeI NdeI NgoMIV PflMI ScaI SfcI TatI TstI
XbaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmrI BmtI BplI BpmI BpuEI BsaAI BsaWI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI
EagI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MluI MspA1I NarI
NcoI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII Tth111I XcmI XhoI
XmaI XmnI