(Linear) (Six-base) MAPSORT of: KIBB0037.seq Check: 4957 from: 1 to: 1413 KIBB0037.orf With 182 enzymes: SgfI * October 24, 2006 15:18 .. AatII G_ACGT'C Cuts at: 0 653 1413 Size: 653 760 AccI GT'mk_AC Cuts at: 0 1289 1413 Size: 1289 124 Acc65I G'GTAC_C Cuts at: 0 1146 1413 Size: 1146 267 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 775 1413 Size: 604 171 638 Fragments arranged by size: 638 604 171 AleI CACnn'nnGTG Cuts at: 0 968 1413 Size: 968 445 ApoI r'AATT_y Cuts at: 0 529 549 1376 1413 Size: 529 20 827 37 Fragments arranged by size: 827 529 37 20 AvaI C'yCGr_G Cuts at: 0 734 1413 Size: 734 679 BamHI G'GATC_C Cuts at: 0 1238 1413 Size: 1238 175 BanI G'GyrC_C Cuts at: 0 115 1146 1413 Size: 115 1031 267 Fragments arranged by size: 1031 267 115 BanII G_rGCy'C Cuts at: 0 1222 1413 Size: 1222 191 BbsI GAAGACnn'nnnn_ Cuts at: 0 645 1130 1413 Size: 645 485 283 Fragments arranged by size: 645 485 283 BbvCI CC'TCA_GC Cuts at: 0 1002 1413 Size: 1002 411 BglII A'GATC_T Cuts at: 0 809 1413 Size: 809 604 Bme1580I G_kGCm'C Cuts at: 0 836 1413 Size: 836 577 BmgBI CAC'GTC Cuts at: 0 660 1039 1413 Size: 660 379 374 Fragments arranged by size: 660 379 374 Bpu10I CC'TnA_GC Cuts at: 0 1002 1413 Size: 1002 411 BsaI GGTCTCn'nnnn_ Cuts at: 0 406 640 1413 Size: 406 234 773 Fragments arranged by size: 773 406 234 BsaBI GATnn'nnATC Cuts at: 0 16 269 1013 1413 Size: 16 253 744 400 Fragments arranged by size: 744 400 253 16 BsaHI Gr'CG_yC Cuts at: 0 650 1413 Size: 650 763 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 955 1413 Size: 955 458 BseYI C'CCAG_C Cuts at: 0 202 1413 Size: 202 1211 BsmI GAATG_Cn' Cuts at: 0 497 1413 Size: 497 916 Bsp1286I G_dGCh'C Cuts at: 0 836 1222 1413 Size: 836 386 191 Fragments arranged by size: 836 386 191 BsrDI GCAATG_nn' Cuts at: 0 79 746 1413 Size: 79 667 667 Fragments arranged by size: 667 667 79 BsrFI r'CCGG_y Cuts at: 0 52 1413 Size: 52 1361 BstYI r'GATC_y Cuts at: 0 809 1238 1272 1413 Size: 809 429 34 141 Fragments arranged by size: 809 429 141 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1000 1413 Size: 1000 413 DraI TTT'AAA Cuts at: 0 371 1413 Size: 371 1042 EaeI y'GGCC_r Cuts at: 0 2 50 1413 Size: 2 48 1363 Fragments arranged by size: 1363 48 2 EarI CTCTTCn'nnn_ Cuts at: 0 323 905 1413 Size: 323 582 508 Fragments arranged by size: 582 508 323 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 695 1413 Size: 695 718 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 775 1413 Size: 604 171 638 Fragments arranged by size: 638 604 171 EcoNI CCTnn'n_nnAGG Cuts at: 0 152 1413 Size: 152 1261 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1012 1413 Size: 1012 401 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 888 920 1307 1330 1339 1362 1413 Size: 888 32 387 23 9 23 51 Fragments arranged by size: 888 387 51 32 23 23 9 KpnI G_GTAC'C Cuts at: 0 1150 1413 Size: 1150 263 MfeI C'AATT_G Cuts at: 0 557 1413 Size: 557 856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 314 1413 Size: 314 1099 MscI TGG'CCA Cuts at: 0 4 1413 Size: 4 1409 MslI CAynn'nnrTG Cuts at: 0 421 968 1413 Size: 421 547 445 Fragments arranged by size: 547 445 421 NaeI GCC'GGC Cuts at: 0 54 1413 Size: 54 1359 NdeI CA'TA_TG Cuts at: 0 822 1413 Size: 822 591 NgoMIV G'CCGG_C Cuts at: 0 52 1413 Size: 52 1361 PflMI CCAn_nnn'nTGG Cuts at: 0 663 1413 Size: 663 750 ScaI AGT'ACT Cuts at: 0 796 1413 Size: 796 617 SfcI C'TryA_G Cuts at: 0 1286 1413 Size: 1286 127 TatI w'GTAC_w Cuts at: 0 794 1413 Size: 794 619 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1215 1247 1413 Size: 1215 32 166 Fragments arranged by size: 1215 166 32 XbaI T'CTAG_A Cuts at: 0 690 1413 Size: 690 723 ZraI GAC'GTC Cuts at: 0 651 1413 Size: 651 762 Enzymes that do cut: AatII AccI Acc65I AcuI AleI ApoI AvaI BamHI BanI BanII BbsI BbvCI BglII Bme1580I BmgBI Bpu10I BsaI BsaBI BsaHI BseRI BseYI BsmI Bsp1286I BsrDI BsrFI BstYI BtgZI DraI EaeI EarI EciI Eco57MI EcoNI FalI Hin4I KpnI MfeI MmeI MscI MslI NaeI NdeI NgoMIV PflMI ScaI SfcI TatI TstI XbaI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI BpmI BpuEI BsaAI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EagI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI MluI MspA1I NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XcmI XhoI XmaI XmnI