Restriction map for
pF1KSDB0041
(Linear) (Six-base) MAPSORT of: KIBB0041.seq Check: 3090 from: 1 to: 1389
KIBB0041.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
Acc65I G'GTAC_C
Cuts at: 0 460 1285 1389
Size: 460 825 104
Fragments arranged by size:
825 460 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 779 1178 1389
Size: 737 42 399 211
Fragments arranged by size:
737 399 211 42
AhdI GACnn_n'nnGTC
Cuts at: 0 155 1389
Size: 155 1234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 416 1389
Size: 416 973
AlwNI CAG_nnn'CTG
Cuts at: 0 631 1389
Size: 631 758
ApoI r'AATT_y
Cuts at: 0 871 1389
Size: 871 518
AvaI C'yCGr_G
Cuts at: 0 170 1301 1389
Size: 170 1131 88
Fragments arranged by size:
1131 170 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 444 477 1389
Size: 444 33 912
Fragments arranged by size:
912 444 33
BanI G'GyrC_C
Cuts at: 0 10 460 1285 1389
Size: 10 450 825 104
Fragments arranged by size:
825 450 104 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 1389
Size: 146 1243
BbvCI CC'TCA_GC
Cuts at: 0 606 1389
Size: 606 783
BciVI GTATCCnnnnn_n'
Cuts at: 0 498 1216 1389
Size: 498 718 173
Fragments arranged by size:
718 498 173
BglII A'GATC_T
Cuts at: 0 511 1389
Size: 511 878
Bme1580I G_kGCm'C
Cuts at: 0 13 1389
Size: 13 1376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 767 1389
Size: 608 159 622
Fragments arranged by size:
622 608 159
Bpu10I CC'TnA_GC
Cuts at: 0 606 1389
Size: 606 783
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 518 528 994 1389
Size: 248 270 10 466 395
Fragments arranged by size:
466 395 270 248 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 1137 1389
Size: 1137 252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 630 660 798 828 1389
Size: 630 30 138 30 561
Fragments arranged by size:
630 561 138 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1250 1340 1389
Size: 1250 90 49
Fragments arranged by size:
1250 90 49
BseYI C'CCAG_C
Cuts at: 0 270 1389
Size: 270 1119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1389
Size: 616 773
BsiHKAI G_wGCw'C
Cuts at: 0 707 792 1389
Size: 707 85 597
Fragments arranged by size:
707 597 85
Bsp1286I G_dGCh'C
Cuts at: 0 13 707 792 1389
Size: 13 694 85 597
Fragments arranged by size:
694 597 85 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 627 1389
Size: 627 762
BsrBI CCG'CTC
Cuts at: 0 5 1389
Size: 5 1384
BstYI r'GATC_y
Cuts at: 0 56 511 1062 1389
Size: 56 455 551 327
Fragments arranged by size:
551 455 327 56
BtgI C'CryG_G
Cuts at: 0 201 320 581 1209 1215 1251 1389
Size: 201 119 261 628 6 36 138
Fragments arranged by size:
628 261 201 138 119 36 6
BtsI GCAGTG_nn'
Cuts at: 0 599 1292 1389
Size: 599 693 97
Fragments arranged by size:
693 599 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1362 1389
Size: 1362 27
EaeI y'GGCC_r
Cuts at: 0 1212 1389
Size: 1212 177
EarI CTCTTCn'nnn_
Cuts at: 0 917 1389
Size: 917 472
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 1389
Size: 67 1322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 737 767 779 1178 1389
Size: 608 129 30 12 399 211
Fragments arranged by size:
608 399 211 129 30 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 988 1084 1389
Size: 988 96 305
Fragments arranged by size:
988 305 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 164 173 196 496 528 630 662
Size: 141 23 9 23 300 32 102 32
Cuts at: 662 1389
Size: 727
Fragments arranged by size:
727 300 141 102 32 32 23 23
9
KpnI G_GTAC'C
Cuts at: 0 464 1289 1389
Size: 464 825 100
Fragments arranged by size:
825 464 100
MslI CAynn'nnrTG
Cuts at: 0 827 1389
Size: 827 562
MspA1I CmG'CkG
Cuts at: 0 631 1389
Size: 631 758
NcoI C'CATG_G
Cuts at: 0 320 581 1389
Size: 320 261 808
Fragments arranged by size:
808 320 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 1210 1389
Size: 321 889 179
Fragments arranged by size:
889 321 179
PspXI vC'TCGA_Gb
Cuts at: 0 1301 1389
Size: 1301 88
PvuII CAG'CTG
Cuts at: 0 631 1389
Size: 631 758
SmlI C'TyrA_G
Cuts at: 0 170 263 533 543 973 1301 1389
Size: 170 93 270 10 430 328 88
Fragments arranged by size:
430 328 270 170 93 88 10
SspI AAT'ATT
Cuts at: 0 1116 1389
Size: 1116 273
StuI AGG'CCT
Cuts at: 0 663 1389
Size: 663 726
StyI C'CwwG_G
Cuts at: 0 47 320 581 802 1389
Size: 47 273 261 221 587
Fragments arranged by size:
587 273 261 221 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 1389
Size: 162 1227
TatI w'GTAC_w
Cuts at: 0 720 1389
Size: 720 669
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 798 830 1389
Size: 798 32 559
Fragments arranged by size:
798 559 32
XhoI C'TCGA_G
Cuts at: 0 170 1301 1389
Size: 170 1131 88
Fragments arranged by size:
1131 170 88
Enzymes that do cut:
Acc65I AcuI AhdI AlfI AlwNI ApoI AvaI BaeI
BanI BbsI BbvCI BciVI BglII Bme1580I BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BstYI BtgI BtsI CspCI EaeI EarI
EciI Eco57MI FalI Hin4I KpnI MslI MspA1I NcoI
PflMI PspXI PvuII SmlI SspI StuI StyI TaqII
TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanII BbeI BcgI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XcmI XmaI XmnI
ZraI