Restriction map for
pF1KB0044
(Linear) (Six-base) MAPSORT of: KIBB0044.seq Check: 8504 from: 1 to: 3774
KIBB0044.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
Acc65I G'GTAC_C
Cuts at: 0 1636 3774
Size: 1636 2138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 796 1063 2425 3774
Size: 476 320 267 1362 1349
Fragments arranged by size:
1362 1349 476 320 267
AflIII A'CryG_T
Cuts at: 0 1878 3774
Size: 1878 1896
AleI CACnn'nnGTG
Cuts at: 0 2214 3774
Size: 2214 1560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 844 1666 1856 2672 3774
Size: 844 822 190 816 1102
Fragments arranged by size:
1102 844 822 816 190
AlwNI CAG_nnn'CTG
Cuts at: 0 2337 2467 3774
Size: 2337 130 1307
Fragments arranged by size:
2337 1307 130
ApoI r'AATT_y
Cuts at: 0 460 517 538 2747 3025 3082 3147 3643
Size: 460 57 21 2209 278 57 65 496
Cuts at: 3643 3774
Size: 131
Fragments arranged by size:
2209 496 460 278 131 65 57 57
21
AseI AT'TA_AT
Cuts at: 0 2416 3774
Size: 2416 1358
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2968 3001 3774
Size: 2968 33 773
Fragments arranged by size:
2968 773 33
BanI G'GyrC_C
Cuts at: 0 1636 3774
Size: 1636 2138
BanII G_rGCy'C
Cuts at: 0 1474 3587 3774
Size: 1474 2113 187
Fragments arranged by size:
2113 1474 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 1238 2027 2167 3494 3774
Size: 1238 789 140 1327 280
Fragments arranged by size:
1327 1238 789 280 140
BbvCI CC'TCA_GC
Cuts at: 0 1610 3774
Size: 1610 2164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3538 3572 3774
Size: 3538 34 202
Fragments arranged by size:
3538 202 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2996 3774
Size: 2996 778
BfrBI ATG'CAT
Cuts at: 0 2649 3774
Size: 2649 1125
BglII A'GATC_T
Cuts at: 0 3458 3774
Size: 3458 316
BlpI GC'TnA_GC
Cuts at: 0 3581 3701 3774
Size: 3581 120 73
Fragments arranged by size:
3581 120 73
BmgBI CAC'GTC
Cuts at: 0 2869 3774
Size: 2869 905
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1503 3170 3774
Size: 286 1217 1667 604
Fragments arranged by size:
1667 1217 604 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1313 1607 2339 3560 3774
Size: 143 1170 294 732 1221 214
Fragments arranged by size:
1221 1170 732 294 214 143
Bpu10I CC'TnA_GC
Cuts at: 0 1610 3706 3774
Size: 1610 2096 68
Fragments arranged by size:
2096 1610 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1460 3774
Size: 1460 2314
BsaI GGTCTCn'nnnn_
Cuts at: 0 3383 3774
Size: 3383 391
BsaAI yAC'GTr
Cuts at: 0 1881 3774
Size: 1881 1893
BsaBI GATnn'nnATC
Cuts at: 0 3457 3774
Size: 3457 317
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 209 1374 2476 2542 3774
Size: 209 1165 1102 66 1232
Fragments arranged by size:
1232 1165 1102 209 66
BsiHKAI G_wGCw'C
Cuts at: 0 1474 1572 3774
Size: 1474 98 2202
Fragments arranged by size:
2202 1474 98
BsmI GAATG_Cn'
Cuts at: 0 435 3767 3774
Size: 435 3332 7
Fragments arranged by size:
3332 435 7
Bsp1286I G_dGCh'C
Cuts at: 0 1474 1572 3587 3774
Size: 1474 98 2015 187
Fragments arranged by size:
2015 1474 187 98
BspHI T'CATG_A
Cuts at: 0 686 1017 3774
Size: 686 331 2757
Fragments arranged by size:
2757 686 331
BsrDI GCAATG_nn'
Cuts at: 0 296 1553 3774
Size: 296 1257 2221
Fragments arranged by size:
2221 1257 296
BsrFI r'CCGG_y
Cuts at: 0 122 1639 3774
Size: 122 1517 2135
Fragments arranged by size:
2135 1517 122
BsrGI T'GTAC_A
Cuts at: 0 1591 3774
Size: 1591 2183
BssSI C'ACGA_G
Cuts at: 0 2909 3774
Size: 2909 865
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2211 3774
Size: 2211 1563
BstBI TT'CG_AA
Cuts at: 0 417 3774
Size: 417 3357
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2848 3774
Size: 2848 926
BstYI r'GATC_y
Cuts at: 0 2130 3458 3774
Size: 2130 1328 316
Fragments arranged by size:
2130 1328 316
BtgI C'CryG_G
Cuts at: 0 204 299 3383 3656 3774
Size: 204 95 3084 273 118
Fragments arranged by size:
3084 273 204 118 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 1255 3774
Size: 327 928 2519
Fragments arranged by size:
2519 928 327
BtsI GCAGTG_nn'
Cuts at: 0 1938 2209 2221 2338 3774
Size: 1938 271 12 117 1436
Fragments arranged by size:
1938 1436 271 117 12
ClaI AT'CG_AT
Cuts at: 0 2001 3774
Size: 2001 1773
DraI TTT'AAA
Cuts at: 0 2958 3774
Size: 2958 816
DraIII CAC_nnn'GTG
Cuts at: 0 1257 3774
Size: 1257 2517
DrdI GACnn_nn'nnGTC
Cuts at: 0 627 640 3774
Size: 627 13 3134
Fragments arranged by size:
3134 627 13
EaeI y'GGCC_r
Cuts at: 0 120 3774
Size: 120 3654
EarI CTCTTCn'nnn_
Cuts at: 0 163 204 775 1019 3774
Size: 163 41 571 244 2755
Fragments arranged by size:
2755 571 244 163 41
EcoICRI GAG'CTC
Cuts at: 0 1472 3774
Size: 1472 2302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 476 796 1063 1313 1607 2339 2425
Size: 143 333 320 267 250 294 732 86
Cuts at: 2425 3560 3774
Size: 1135 214
Fragments arranged by size:
1135 732 333 320 294 267 250 214
143 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1532 3774
Size: 1532 2242
EcoO109I rG'GnC_Cy
Cuts at: 0 2436 2815 3774
Size: 2436 379 959
Fragments arranged by size:
2436 959 379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1237 3774
Size: 1237 2537
FseI GG_CCGG'CC
Cuts at: 0 126 3774
Size: 126 3648
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2818 2850 3166 3198 3306 3338 3774
Size: 2818 32 316 32 108 32 436
Fragments arranged by size:
2818 436 316 108 32 32 32
HincII GTy'rAC
Cuts at: 0 1962 2093 2643 3774
Size: 1962 131 550 1131
Fragments arranged by size:
1962 1131 550 131
HpaI GTT'AAC
Cuts at: 0 2093 3774
Size: 2093 1681
KpnI G_GTAC'C
Cuts at: 0 1640 3774
Size: 1640 2134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 3774
Size: 779 2995
MslI CAynn'nnrTG
Cuts at: 0 59 1208 1396 1436 1838 2214 2846 2936
Size: 59 1149 188 40 402 376 632 90
Cuts at: 2936 3529 3774
Size: 593 245
Fragments arranged by size:
1149 632 593 402 376 245 188 90
59 40
MspA1I CmG'CkG
Cuts at: 0 1291 1980 2656 3774
Size: 1291 689 676 1118
Fragments arranged by size:
1291 1118 689 676
NaeI GCC'GGC
Cuts at: 0 124 3774
Size: 124 3650
NcoI C'CATG_G
Cuts at: 0 3383 3656 3774
Size: 3383 273 118
Fragments arranged by size:
3383 273 118
NgoMIV G'CCGG_C
Cuts at: 0 122 3774
Size: 122 3652
NsiI A_TGCA'T
Cuts at: 0 2651 3774
Size: 2651 1123
NspI r_CATG'y
Cuts at: 0 1441 2649 2653 3553 3774
Size: 1441 1208 4 900 221
Fragments arranged by size:
1441 1208 900 221 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 2467 2541 3774
Size: 2467 74 1233
Fragments arranged by size:
2467 1233 74
PpuMI rG'GwC_Cy
Cuts at: 0 2815 3774
Size: 2815 959
PstI C_TGCA'G
Cuts at: 0 1064 1752 1909 2216 2661 3732 3774
Size: 1064 688 157 307 445 1071 42
Fragments arranged by size:
1071 1064 688 445 307 157 42
PvuII CAG'CTG
Cuts at: 0 1291 1980 2656 3774
Size: 1291 689 676 1118
Fragments arranged by size:
1291 1118 689 676
SacI G_AGCT'C
Cuts at: 0 1474 3774
Size: 1474 2300
SapI GCTCTTCn'nnn_
Cuts at: 0 163 204 3774
Size: 163 41 3570
Fragments arranged by size:
3570 163 41
ScaI AGT'ACT
Cuts at: 0 3017 3774
Size: 3017 757
SfcI C'TryA_G
Cuts at: 0 1060 1748 1809 1905 1917 2148 2212 2657
Size: 1060 688 61 96 12 231 64 445
Cuts at: 2657 3141 3728 3774
Size: 484 587 46
Fragments arranged by size:
1060 688 587 484 445 231 96 64
61 46 12
SmlI C'TyrA_G
Cuts at: 0 1475 3774
Size: 1475 2299
SphI G_CATG'C
Cuts at: 0 2653 3553 3774
Size: 2653 900 221
Fragments arranged by size:
2653 900 221
StuI AGG'CCT
Cuts at: 0 2581 3774
Size: 2581 1193
StyI C'CwwG_G
Cuts at: 0 3383 3656 3774
Size: 3383 273 118
Fragments arranged by size:
3383 273 118
SwaI ATTT'AAAT
Cuts at: 0 2958 3774
Size: 2958 816
TatI w'GTAC_w
Cuts at: 0 1259 1591 3015 3360 3774
Size: 1259 332 1424 345 414
Fragments arranged by size:
1424 1259 414 345 332
Tth111I GACn'n_nGTC
Cuts at: 0 563 3774
Size: 563 3211
XbaI T'CTAG_A
Cuts at: 0 1896 3774
Size: 1896 1878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 61 795 3774
Size: 61 734 2979
Fragments arranged by size:
2979 734 61
XmnI GAAnn'nnTTC
Cuts at: 0 1178 1529 2801 3774
Size: 1178 351 1272 973
Fragments arranged by size:
1272 1178 973 351
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AlwNI ApoI AseI
BaeI BanI BanII BbsI BbvCI BcgI BciVI BfrBI
BglII BlpI BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BseRI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FseI Hin4I HincII
HpaI KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PflMI PpuMI PstI PvuII SacI SapI
ScaI SfcI SmlI SphI StuI StyI SwaI TatI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI
BbeI BclI BglI Bme1580I BmtI BplI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BssHII BstEII BstZ17I Bsu36I CspCI EagI EciI
EcoRI EcoRV FspI FspAI HaeII HindIII KasI MfeI
MluI MscI NarI NdeI NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI TaqII TstI XhoI XmaI ZraI