Restriction map for
pF1KB0045
(Linear) (Six-base) MAPSORT of: KIBB0045.seq Check: 8826 from: 1 to: 4299
KIBB0045.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4152 4299
Size: 4152 147
AccI GT'mk_AC
Cuts at: 0 1875 4299
Size: 1875 2424
Acc65I G'GTAC_C
Cuts at: 0 2218 4299
Size: 2218 2081
AclI AA'CG_TT
Cuts at: 0 639 4299
Size: 639 3660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2105 3032 4148 4299
Size: 2105 927 1116 151
Fragments arranged by size:
2105 1116 927 151
AflIII A'CryG_T
Cuts at: 0 1870 3064 4299
Size: 1870 1194 1235
Fragments arranged by size:
1870 1235 1194
AleI CACnn'nnGTG
Cuts at: 0 618 2989 4299
Size: 618 2371 1310
Fragments arranged by size:
2371 1310 618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 905 4299
Size: 905 3394
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3445 3477 4299
Size: 3445 32 822
Fragments arranged by size:
3445 822 32
AlwNI CAG_nnn'CTG
Cuts at: 0 136 634 811 814 2350 4299
Size: 136 498 177 3 1536 1949
Fragments arranged by size:
1949 1536 498 177 136 3
ApaLI G'TGCA_C
Cuts at: 0 890 4299
Size: 890 3409
ApoI r'AATT_y
Cuts at: 0 408 732 980 1008 1174 1284 1576 2026
Size: 408 324 248 28 166 110 292 450
Cuts at: 2026 2239 2584 2640 2647 2725 2752 3282 3306
Size: 213 345 56 7 78 27 530 24
Cuts at: 3306 4080 4131 4299
Size: 774 51 168
Fragments arranged by size:
774 530 450 408 345 324 292 248
213 168 166 110 78 56 51 28
27 24 7
AvaI C'yCGr_G
Cuts at: 0 6 2425 4170 4299
Size: 6 2419 1745 129
Fragments arranged by size:
2419 1745 129 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2939 2972 3665 3698 4299
Size: 2939 33 693 33 601
Fragments arranged by size:
2939 693 601 33 33
BamHI G'GATC_C
Cuts at: 0 3775 4299
Size: 3775 524
BanI G'GyrC_C
Cuts at: 0 852 2218 3868 4299
Size: 852 1366 1650 431
Fragments arranged by size:
1650 1366 852 431
BanII G_rGCy'C
Cuts at: 0 280 2145 3399 4299
Size: 280 1865 1254 900
Fragments arranged by size:
1865 1254 900 280
BbsI GAAGACnn'nnnn_
Cuts at: 0 870 1189 2306 4299
Size: 870 319 1117 1993
Fragments arranged by size:
1993 1117 870 319
BbvCI CC'TCA_GC
Cuts at: 0 3804 4152 4299
Size: 3804 348 147
Fragments arranged by size:
3804 348 147
BclI T'GATC_A
Cuts at: 0 3417 4299
Size: 3417 882
BfrBI ATG'CAT
Cuts at: 0 1907 4299
Size: 1907 2392
BglI GCCn_nnn'nGGC
Cuts at: 0 159 4299
Size: 159 4140
BlpI GC'TnA_GC
Cuts at: 0 878 1754 4299
Size: 878 876 2545
Fragments arranged by size:
2545 878 876
Bme1580I G_kGCm'C
Cuts at: 0 894 4299
Size: 894 3405
BmrI ACTGGGnnnn_n'
Cuts at: 0 1768 2842 4299
Size: 1768 1074 1457
Fragments arranged by size:
1768 1457 1074
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 294 3159 4299
Size: 294 2865 1140
Fragments arranged by size:
2865 1140 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 1405 1864 2411 4181 4299
Size: 1340 65 459 547 1770 118
Fragments arranged by size:
1770 1340 547 459 118 65
Bpu10I CC'TnA_GC
Cuts at: 0 308 3804 4152 4299
Size: 308 3496 348 147
Fragments arranged by size:
3496 348 308 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 3623 4299
Size: 1499 2124 676
Fragments arranged by size:
2124 1499 676
BsaBI GATnn'nnATC
Cuts at: 0 1036 3903 4299
Size: 1036 2867 396
Fragments arranged by size:
2867 1036 396
BsaWI w'CCGG_w
Cuts at: 0 47 4299
Size: 47 4252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1476 1506 2958 2988 4299
Size: 1476 30 1452 30 1311
Fragments arranged by size:
1476 1452 1311 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 32 286 992 1004 1402 1405 2562 4299
Size: 32 254 706 12 398 3 1157 1737
Fragments arranged by size:
1737 1157 706 398 254 32 12 3
BseYI C'CCAG_C
Cuts at: 0 1846 2435 4157 4299
Size: 1846 589 1722 142
Fragments arranged by size:
1846 1722 589 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1230 2187 2479 3446 3929 4299
Size: 1230 957 292 967 483 370
Fragments arranged by size:
1230 967 957 483 370 292
BsiHKAI G_wGCw'C
Cuts at: 0 280 894 3399 4299
Size: 280 614 2505 900
Fragments arranged by size:
2505 900 614 280
BsmI GAATG_Cn'
Cuts at: 0 113 463 688 4299
Size: 113 350 225 3611
Fragments arranged by size:
3611 350 225 113
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1341 1517 4299
Size: 1341 176 2782
Fragments arranged by size:
2782 1341 176
Bsp1286I G_dGCh'C
Cuts at: 0 280 894 2145 3399 4299
Size: 280 614 1251 1254 900
Fragments arranged by size:
1254 1251 900 614 280
BspEI T'CCGG_A
Cuts at: 0 47 4299
Size: 47 4252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2358 2923 4152 4299
Size: 2358 565 1229 147
Fragments arranged by size:
2358 1229 565 147
BsrDI GCAATG_nn'
Cuts at: 0 607 4299
Size: 607 3692
BstAPI GCAn_nnn'nTGC
Cuts at: 0 811 3814 3832 4299
Size: 811 3003 18 467
Fragments arranged by size:
3003 811 467 18
BstBI TT'CG_AA
Cuts at: 0 2557 4299
Size: 2557 1742
BstXI CCAn_nnnn'nTGG
Cuts at: 0 620 2487 4299
Size: 620 1867 1812
Fragments arranged by size:
1867 1812 620
BstYI r'GATC_y
Cuts at: 0 44 2257 3775 3898 4299
Size: 44 2213 1518 123 401
Fragments arranged by size:
2213 1518 401 123 44
Bsu36I CC'TnA_GG
Cuts at: 0 2223 4299
Size: 2223 2076
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1712 4299
Size: 1712 2587
BtsI GCAGTG_nn'
Cuts at: 0 662 1447 1867 3599 4299
Size: 662 785 420 1732 700
Fragments arranged by size:
1732 785 700 662 420
DraI TTT'AAA
Cuts at: 0 1149 1575 1841 2031 2529 2646 2724 2751
Size: 1149 426 266 190 498 117 78 27
Cuts at: 2751 4299
Size: 1548
Fragments arranged by size:
1548 1149 498 426 266 190 117 78
27
EaeI y'GGCC_r
Cuts at: 0 232 4299
Size: 232 4067
EarI CTCTTCn'nnn_
Cuts at: 0 395 2610 3179 3242 3528 4245 4299
Size: 395 2215 569 63 286 717 54
Fragments arranged by size:
2215 717 569 395 286 63 54
EcoICRI GAG'CTC
Cuts at: 0 278 3397 4299
Size: 278 3119 902
Fragments arranged by size:
3119 902 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 1405 1864 2105 2411 3032 4148 4181
Size: 1340 65 459 241 306 621 1116 33
Cuts at: 4181 4299
Size: 118
Fragments arranged by size:
1340 1116 621 459 306 241 118 65
33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 2390 4299
Size: 496 1894 1909
Fragments arranged by size:
1909 1894 496
EcoRI G'AATT_C
Cuts at: 0 732 980 1008 1174 3282 3306 4299
Size: 732 248 28 166 2108 24 993
Fragments arranged by size:
2108 993 732 248 166 28 24
EcoRV GAT'ATC
Cuts at: 0 1038 4299
Size: 1038 3261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3030 4299
Size: 3030 1269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 526 534 558 937 969 2680 2712
Size: 502 24 8 24 379 32 1711 32
Cuts at: 2712 3343 3375 4299
Size: 631 32 924
Fragments arranged by size:
1711 924 631 502 379 32 32 32
24 24 8
HincII GTy'rAC
Cuts at: 0 377 3262 4299
Size: 377 2885 1037
Fragments arranged by size:
2885 1037 377
HpaI GTT'AAC
Cuts at: 0 377 4299
Size: 377 3922
KpnI G_GTAC'C
Cuts at: 0 2222 4299
Size: 2222 2077
MfeI C'AATT_G
Cuts at: 0 470 4299
Size: 470 3829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 401 4299
Size: 3 398 3898
Fragments arranged by size:
3898 398 3
MscI TGG'CCA
Cuts at: 0 234 4299
Size: 234 4065
MslI CAynn'nnrTG
Cuts at: 0 530 618 1860 2989 4299
Size: 530 88 1242 1129 1310
Fragments arranged by size:
1310 1242 1129 530 88
MspA1I CmG'CkG
Cuts at: 0 200 811 2201 3415 3679 4299
Size: 200 611 1390 1214 264 620
Fragments arranged by size:
1390 1214 620 611 264 200
NsiI A_TGCA'T
Cuts at: 0 1909 4299
Size: 1909 2390
NspI r_CATG'y
Cuts at: 0 1874 2495 3068 4299
Size: 1874 621 573 1231
Fragments arranged by size:
1874 1231 621 573
PciI A'CATG_T
Cuts at: 0 1870 3064 4299
Size: 1870 1194 1235
Fragments arranged by size:
1870 1235 1194
PflMI CCAn_nnn'nTGG
Cuts at: 0 3829 3853 3895 4299
Size: 3829 24 42 404
Fragments arranged by size:
3829 404 42 24
PfoI T'CCnGG_A
Cuts at: 0 780 3702 4299
Size: 780 2922 597
Fragments arranged by size:
2922 780 597
PsiI TTA'TAA
Cuts at: 0 1708 4299
Size: 1708 2591
PstI C_TGCA'G
Cuts at: 0 1302 2934 3774 4149 4299
Size: 1302 1632 840 375 150
Fragments arranged by size:
1632 1302 840 375 150
PvuII CAG'CTG
Cuts at: 0 200 811 2201 3415 3679 4299
Size: 200 611 1390 1214 264 620
Fragments arranged by size:
1390 1214 620 611 264 200
SacI G_AGCT'C
Cuts at: 0 280 3399 4299
Size: 280 3119 900
Fragments arranged by size:
3119 900 280
SapI GCTCTTCn'nnn_
Cuts at: 0 3242 4299
Size: 3242 1057
ScaI AGT'ACT
Cuts at: 0 351 1215 1692 3408 4299
Size: 351 864 477 1716 891
Fragments arranged by size:
1716 891 864 477 351
SfcI C'TryA_G
Cuts at: 0 1298 2930 3410 3770 4145 4299
Size: 1298 1632 480 360 375 154
Fragments arranged by size:
1632 1298 480 375 360 154
SmlI C'TyrA_G
Cuts at: 0 1514 3638 4299
Size: 1514 2124 661
Fragments arranged by size:
2124 1514 661
SpeI A'CTAG_T
Cuts at: 0 346 3403 4299
Size: 346 3057 896
Fragments arranged by size:
3057 896 346
SspI AAT'ATT
Cuts at: 0 147 285 932 4299
Size: 147 138 647 3367
Fragments arranged by size:
3367 647 147 138
StuI AGG'CCT
Cuts at: 0 384 4299
Size: 384 3915
StyI C'CwwG_G
Cuts at: 0 3021 4299
Size: 3021 1278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1515 1950 4299
Size: 1515 435 2349
Fragments arranged by size:
2349 1515 435
TatI w'GTAC_w
Cuts at: 0 349 1213 1614 1690 3406 3475 4299
Size: 349 864 401 76 1716 69 824
Fragments arranged by size:
1716 864 824 401 349 76 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1548 1580 2117 2149 2958 2990 4299
Size: 1548 32 537 32 809 32 1309
Fragments arranged by size:
1548 1309 809 537 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3860 3966 4299
Size: 3860 106 333
Fragments arranged by size:
3860 333 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3537 4299
Size: 3537 762
XmnI GAAnn'nnTTC
Cuts at: 0 714 4299
Size: 714 3585
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BclI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BstAPI BstBI BstXI BstYI Bsu36I BtgZI
BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI Hin4I HincII HpaI KpnI MfeI MmeI
MscI MslI MspA1I NsiI NspI PciI PflMI PfoI
PsiI PstI PvuII SacI SapI ScaI SfcI SmlI
SpeI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI ApaI AscI AseI
AsiSI AvrII BbeI BcgI BciVI BglII BmgBI BmtI
BsaI BsaAI BsaHI BsiEI BsiWI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstZ17I BtgI ClaI CspCI
DraIII DrdI EagI EciI EcoO109I FseI FspI FspAI
HaeII HindIII KasI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI XbaI XhoI
XmaI ZraI