Restriction map for
pF1KB0047
(Linear) (Six-base) MAPSORT of: KIBB0047.seq Check: 6909 from: 1 to: 6021
KIBB0047.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4701 6021
Size: 4701 1320
AccI GT'mk_AC
Cuts at: 0 5679 6021
Size: 5679 342
AclI AA'CG_TT
Cuts at: 0 4670 6021
Size: 4670 1351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1067 2638 2825 3161 3445 3649 3920
Size: 334 733 1571 187 336 284 204 271
Cuts at: 3920 4048 4120 4306 4990 5503 5884 6021
Size: 128 72 186 684 513 381 137
Fragments arranged by size:
1571 733 684 513 381 336 334 284
271 204 187 186 137 128 72
AflIII A'CryG_T
Cuts at: 0 5773 6021
Size: 5773 248
AhdI GACnn_n'nnGTC
Cuts at: 0 1373 2874 6021
Size: 1373 1501 3147
Fragments arranged by size:
3147 1501 1373
AleI CACnn'nnGTG
Cuts at: 0 2611 6021
Size: 2611 3410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2549 6021
Size: 2549 3472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2327 2359 4655 4687 6021
Size: 2327 32 2296 32 1334
Fragments arranged by size:
2327 2296 1334 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 544 1485 2791 2955 3249 3777 4314 4377
Size: 544 941 1306 164 294 528 537 63
Cuts at: 4377 5460 6021
Size: 1083 561
Fragments arranged by size:
1306 1083 941 561 544 537 528 294
164 63
ApaI G_GGCC'C
Cuts at: 0 4566 4701 6021
Size: 4566 135 1320
Fragments arranged by size:
4566 1320 135
ApoI r'AATT_y
Cuts at: 0 760 1421 2260 3072 4006 6021
Size: 760 661 839 812 934 2015
Fragments arranged by size:
2015 934 839 812 760 661
AvaI C'yCGr_G
Cuts at: 0 19 1208 3061 4559 4567 4894 5009 5503
Size: 19 1189 1853 1498 8 327 115 494
Cuts at: 5503 6021
Size: 518
Fragments arranged by size:
1853 1498 1189 518 494 327 115 19
8
BamHI G'GATC_C
Cuts at: 0 24 2163 2213 4103 6021
Size: 24 2139 50 1890 1918
Fragments arranged by size:
2139 1918 1890 50 24
BanI G'GyrC_C
Cuts at: 0 5921 6021
Size: 5921 100
BanII G_rGCy'C
Cuts at: 0 2798 2988 3335 3716 3884 4566 4648 4701
Size: 2798 190 347 381 168 682 82 53
Cuts at: 4701 4895 5199 5957 6021
Size: 194 304 758 64
Fragments arranged by size:
2798 758 682 381 347 304 194 190
168 82 64 53
BbeI G_GCGC'C
Cuts at: 0 5925 6021
Size: 5925 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 730 1933 2595 3473 4126 4357 4640
Size: 556 174 1203 662 878 653 231 283
Cuts at: 4640 4754 6021
Size: 114 1267
Fragments arranged by size:
1267 1203 878 662 653 556 283 231
174 114
BbvCI CC'TCA_GC
Cuts at: 0 5875 6021
Size: 5875 146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3273 3307 6021
Size: 3273 34 2714
Fragments arranged by size:
3273 2714 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4041 6021
Size: 4041 1980
BclI T'GATC_A
Cuts at: 0 1131 4874 6021
Size: 1131 3743 1147
Fragments arranged by size:
3743 1147 1131
BfrBI ATG'CAT
Cuts at: 0 491 1583 6021
Size: 491 1092 4438
Fragments arranged by size:
4438 1092 491
BglI GCCn_nnn'nGGC
Cuts at: 0 2503 4582 4810 5551 5827 5915 6021
Size: 2503 2079 228 741 276 88 106
Fragments arranged by size:
2503 2079 741 276 228 106 88
Bme1580I G_kGCm'C
Cuts at: 0 4566 4701 6021
Size: 4566 135 1320
Fragments arranged by size:
4566 1320 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 4200 6006 6021
Size: 4200 1806 15
Fragments arranged by size:
4200 1806 15
BmtI G_CTAG'C
Cuts at: 0 5413 6021
Size: 5413 608
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1452 3144 3732 6021
Size: 1452 1692 588 2289
Fragments arranged by size:
2289 1692 1452 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 2429 2737 3577 3727 3799 4198 4336
Size: 917 1512 308 840 150 72 399 138
Cuts at: 4336 4366 4600 4741 4756 4807 4927 5026 5335
Size: 30 234 141 15 51 120 99 309
Cuts at: 5335 5482 5569 5590 5770 5785 5971 6021
Size: 147 87 21 180 15 186 50
Fragments arranged by size:
1512 917 840 399 309 308 234 186
180 150 147 141 138 120 99 87
72 51 50 30 21 15 15
Bpu10I CC'TnA_GC
Cuts at: 0 3913 4013 4093 4303 5842 5875 6021
Size: 3913 100 80 210 1539 33 146
Fragments arranged by size:
3913 1539 210 146 100 80 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 3319 3543 3661 4051 5602 6021
Size: 62 3257 224 118 390 1551 419
Fragments arranged by size:
3257 1551 419 390 224 118 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1006 1593 3900 4820 6021
Size: 1006 587 2307 920 1201
Fragments arranged by size:
2307 1201 1006 920 587
BsaAI yAC'GTr
Cuts at: 0 3598 6021
Size: 3598 2423
BsaBI GATnn'nnATC
Cuts at: 0 2218 6021
Size: 2218 3803
BsaHI Gr'CG_yC
Cuts at: 0 5289 5922 6021
Size: 5289 633 99
Fragments arranged by size:
5289 633 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 155 185 3787 3817 6021
Size: 155 30 3602 30 2204
Fragments arranged by size:
3602 2204 155 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2734 2989 3061 3373 3376 3658 3724 4084
Size: 2734 255 72 312 3 282 66 360
Cuts at: 4084 4135 4165 4168 4171 4174 4333 4606 4753
Size: 51 30 3 3 3 159 273 147
Cuts at: 4753 5332 5335 5347 5587 6021
Size: 579 3 12 240 434
Fragments arranged by size:
2734 579 434 360 312 282 273 255
240 159 147 72 66 51 30 12
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 544 3584 4730 6021
Size: 544 3040 1146 1291
Fragments arranged by size:
3040 1291 1146 544
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5914 6021
Size: 5914 107
BsiEI CG_ry'CG
Cuts at: 0 1242 3287 5364 6021
Size: 1242 2045 2077 657
Fragments arranged by size:
2077 2045 1242 657
BsiHKAI G_wGCw'C
Cuts at: 0 2798 3335 3716 3848 3884 4648 4895 5957
Size: 2798 537 381 132 36 764 247 1062
Cuts at: 5957 6021
Size: 64
Fragments arranged by size:
2798 1062 764 537 381 247 132 64
36
BsmI GAATG_Cn'
Cuts at: 0 1648 6021
Size: 1648 4373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3274 5992 6021
Size: 3274 2718 29
Fragments arranged by size:
3274 2718 29
Bsp1286I G_dGCh'C
Cuts at: 0 2798 2988 3335 3716 3848 3884 4566 4648
Size: 2798 190 347 381 132 36 682 82
Cuts at: 4648 4701 4895 5199 5957 6021
Size: 53 194 304 758 64
Fragments arranged by size:
2798 758 682 381 347 304 194 190
132 82 64 53 36
BspHI T'CATG_A
Cuts at: 0 441 1944 4640 6021
Size: 441 1503 2696 1381
Fragments arranged by size:
2696 1503 1381 441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1611 3299 3816 3861 4701 4838 5652 6021
Size: 1611 1688 517 45 840 137 814 369
Fragments arranged by size:
1688 1611 840 814 517 369 137 45
BsrBI CCG'CTC
Cuts at: 0 1320 2730 4503 5466 6021
Size: 1320 1410 1773 963 555
Fragments arranged by size:
1773 1410 1320 963 555
BsrDI GCAATG_nn'
Cuts at: 0 212 1031 3113 6021
Size: 212 819 2082 2908
Fragments arranged by size:
2908 2082 819 212
BsrFI r'CCGG_y
Cuts at: 0 5870 5918 6021
Size: 5870 48 103
Fragments arranged by size:
5870 103 48
BsrGI T'GTAC_A
Cuts at: 0 422 2372 6021
Size: 422 1950 3649
Fragments arranged by size:
3649 1950 422
BssSI C'ACGA_G
Cuts at: 0 836 2140 5084 6021
Size: 836 1304 2944 937
Fragments arranged by size:
2944 1304 937 836
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 1285 2830 2997 6021
Size: 684 601 1545 167 3024
Fragments arranged by size:
3024 1545 684 601 167
BstEII G'GTnAC_C
Cuts at: 0 2488 6021
Size: 2488 3533
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1183 2085 3978 6021
Size: 1183 902 1893 2043
Fragments arranged by size:
2043 1893 1183 902
BstYI r'GATC_y
Cuts at: 0 24 1569 2163 2213 2279 4103 6021
Size: 24 1545 594 50 66 1824 1918
Fragments arranged by size:
1918 1824 1545 594 66 50 24
Bsu36I CC'TnA_GG
Cuts at: 0 632 6021
Size: 632 5389
BtgI C'CryG_G
Cuts at: 0 126 1016 3575 4763 6021
Size: 126 890 2559 1188 1258
Fragments arranged by size:
2559 1258 1188 890 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1327 6021
Size: 1327 4694
BtsI GCAGTG_nn'
Cuts at: 0 2596 6021
Size: 2596 3425
ClaI AT'CG_AT
Cuts at: 0 1556 6021
Size: 1556 4465
DraI TTT'AAA
Cuts at: 0 2444 6021
Size: 2444 3577
DrdI GACnn_nn'nnGTC
Cuts at: 0 55 3871 6021
Size: 55 3816 2150
Fragments arranged by size:
3816 2150 55
EaeI y'GGCC_r
Cuts at: 0 1239 1838 2495 3167 3347 3380 5916 6021
Size: 1239 599 657 672 180 33 2536 105
Fragments arranged by size:
2536 1239 672 657 599 180 105 33
EagI C'GGCC_G
Cuts at: 0 1239 6021
Size: 1239 4782
EarI CTCTTCn'nnn_
Cuts at: 0 364 1046 2140 2663 2747 2807 2849 3038
Size: 364 682 1094 523 84 60 42 189
Cuts at: 3038 3137 4112 4463 4468 4493 4850 5134 5324
Size: 99 975 351 5 25 357 284 190
Cuts at: 5324 5431 5907 6021
Size: 107 476 114
Fragments arranged by size:
1094 975 682 523 476 364 357 351
284 190 189 114 107 99 84 60
42 25 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3943 4915 5248 6021
Size: 3943 972 333 773
Fragments arranged by size:
3943 972 773 333
EcoICRI GAG'CTC
Cuts at: 0 2796 3333 3714 3882 4646 4893 5955 6021
Size: 2796 537 381 168 764 247 1062 66
Fragments arranged by size:
2796 1062 764 537 381 247 168 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 917 1067 2429 2638 2737 2825 3161
Size: 334 583 150 1362 209 99 88 336
Cuts at: 3161 3445 3577 3649 3727 3799 3920 4048 4120
Size: 284 132 72 78 72 121 128 72
Cuts at: 4120 4198 4306 4336 4366 4600 4741 4756 4807
Size: 78 108 30 30 234 141 15 51
Cuts at: 4807 4927 4990 5026 5335 5482 5503 5569 5590
Size: 120 63 36 309 147 21 66 21
Cuts at: 5590 5770 5785 5884 5971 6021
Size: 180 15 99 87 50
Fragments arranged by size:
1362 583 336 334 309 284 234 209
180 150 147 141 132 128 121 120
108 99 99 88 87 78 78 72
72 72 66 63 51 50 36 30
30 21 21 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3823 4369 6021
Size: 3823 546 1652
Fragments arranged by size:
3823 1652 546
EcoO109I rG'GnC_Cy
Cuts at: 0 2952 3774 3816 4299 4563 4698 4727 4974
Size: 2952 822 42 483 264 135 29 247
Cuts at: 4974 5838 6021
Size: 864 183
Fragments arranged by size:
2952 864 822 483 264 247 183 135
42 29
EcoRI G'AATT_C
Cuts at: 0 2260 6021
Size: 2260 3761
EcoRV GAT'ATC
Cuts at: 0 2457 6021
Size: 2457 3564
FspI TGC'GCA
Cuts at: 0 1163 6021
Size: 1163 4858
HaeII r_GCGC'y
Cuts at: 0 4314 4413 4933 5255 5429 5925 6021
Size: 4314 99 520 322 174 496 96
Fragments arranged by size:
4314 520 496 322 174 99 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 730 762 1045 1077 1359 1391
Size: 499 32 199 32 283 32 282 32
Cuts at: 1391 2076 2108 2264 2271 2296 2303 2860 2892
Size: 685 32 156 7 25 7 557 32
Cuts at: 2892 3460 3492 3952 3984 5094 5126 6021
Size: 568 32 460 32 1110 32 895
Fragments arranged by size:
1110 895 685 568 557 499 460 283
282 199 156 32 32 32 32 32
32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 213 4794 5680 5994 6021
Size: 213 4581 886 314 27
Fragments arranged by size:
4581 886 314 213 27
HindIII A'AGCT_T
Cuts at: 0 293 1136 4279 4777 6021
Size: 293 843 3143 498 1244
Fragments arranged by size:
3143 1244 843 498 293
KasI G'GCGC_C
Cuts at: 0 5921 6021
Size: 5921 100
MfeI C'AATT_G
Cuts at: 0 4250 5164 6021
Size: 4250 914 857
Fragments arranged by size:
4250 914 857
MluI A'CGCG_T
Cuts at: 0 5773 6021
Size: 5773 248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4081 4448 5984 6021
Size: 4081 367 1536 37
Fragments arranged by size:
4081 1536 367 37
MscI TGG'CCA
Cuts at: 0 1840 2497 3169 3349 3382 6021
Size: 1840 657 672 180 33 2639
Fragments arranged by size:
2639 1840 672 657 180 33
MslI CAynn'nnrTG
Cuts at: 0 461 1990 2611 6021
Size: 461 1529 621 3410
Fragments arranged by size:
3410 1529 621 461
MspA1I CmG'CkG
Cuts at: 0 1485 2553 3003 3135 3345 3726 4113 4458
Size: 1485 1068 450 132 210 381 387 345
Cuts at: 4458 4734 4926 5313 5568 5748 5805 5928 6021
Size: 276 192 387 255 180 57 123 93
Fragments arranged by size:
1485 1068 450 387 387 381 345 276
255 210 192 180 132 123 93 57
NaeI GCC'GGC
Cuts at: 0 5920 6021
Size: 5920 101
NarI GG'CG_CC
Cuts at: 0 5922 6021
Size: 5922 99
NcoI C'CATG_G
Cuts at: 0 126 1016 6021
Size: 126 890 5005
Fragments arranged by size:
5005 890 126
NgoMIV G'CCGG_C
Cuts at: 0 5918 6021
Size: 5918 103
NheI G'CTAG_C
Cuts at: 0 5409 6021
Size: 5409 612
NsiI A_TGCA'T
Cuts at: 0 493 1585 6021
Size: 493 1092 4436
Fragments arranged by size:
4436 1092 493
NspI r_CATG'y
Cuts at: 0 284 495 2040 4800 6021
Size: 284 211 1545 2760 1221
Fragments arranged by size:
2760 1545 1221 284 211
PflMI CCAn_nnn'nTGG
Cuts at: 0 4314 5460 6021
Size: 4314 1146 561
Fragments arranged by size:
4314 1146 561
PfoI T'CCnGG_A
Cuts at: 0 981 1007 1400 2204 2255 3674 3875 6021
Size: 981 26 393 804 51 1419 201 2146
Fragments arranged by size:
2146 1419 981 804 393 201 51 26
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2396 2428 6021
Size: 2396 32 3593
Fragments arranged by size:
3593 2396 32
PpuMI rG'GwC_Cy
Cuts at: 0 2952 3816 4727 4974 6021
Size: 2952 864 911 247 1047
Fragments arranged by size:
2952 1047 911 864 247
PsiI TTA'TAA
Cuts at: 0 373 6021
Size: 373 5648
PspOMI G'GGCC_C
Cuts at: 0 4562 4697 6021
Size: 4562 135 1324
Fragments arranged by size:
4562 1324 135
PspXI vC'TCGA_Gb
Cuts at: 0 19 4567 4894 5503 6021
Size: 19 4548 327 609 518
Fragments arranged by size:
4548 609 518 327 19
PstI C_TGCA'G
Cuts at: 0 1481 1577 3002 3296 3317 3344 3371 3824
Size: 1481 96 1425 294 21 27 27 453
Cuts at: 3824 3950 4202 4370 5498 6021
Size: 126 252 168 1128 523
Fragments arranged by size:
1481 1425 1128 523 453 294 252 168
126 96 27 27 21
PvuII CAG'CTG
Cuts at: 0 1485 3003 3135 3345 3726 4113 4458 4734
Size: 1485 1518 132 210 381 387 345 276
Cuts at: 4734 5313 5568 5748 5928 6021
Size: 579 255 180 180 93
Fragments arranged by size:
1518 1485 579 387 381 345 276 255
210 180 180 132 93
SacI G_AGCT'C
Cuts at: 0 2798 3335 3716 3884 4648 4895 5957 6021
Size: 2798 537 381 168 764 247 1062 64
Fragments arranged by size:
2798 1062 764 537 381 247 168 64
SalI G'TCGA_C
Cuts at: 0 5678 6021
Size: 5678 343
SapI GCTCTTCn'nnn_
Cuts at: 0 2663 4468 4493 6021
Size: 2663 1805 25 1528
Fragments arranged by size:
2663 1805 1528 25
SbfI CC_TGCA'GG
Cuts at: 0 3296 6021
Size: 3296 2725
SfcI C'TryA_G
Cuts at: 0 948 1090 1477 1573 2998 3292 3313 3340
Size: 948 142 387 96 1425 294 21 27
Cuts at: 3340 3367 3820 3946 4042 4198 4366 4937 5383
Size: 27 453 126 96 156 168 571 446
Cuts at: 5383 5494 6021
Size: 111 527
Fragments arranged by size:
1425 948 571 527 453 446 387 294
168 156 142 126 111 96 96 27
27 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2503 6021
Size: 2503 3518
SfoI GGC'GCC
Cuts at: 0 5923 6021
Size: 5923 98
SmaI CCC'GGG
Cuts at: 0 4561 6021
Size: 4561 1460
SmlI C'TyrA_G
Cuts at: 0 19 77 3061 3334 3522 3640 4030 4567
Size: 19 58 2984 273 188 118 390 537
Cuts at: 4567 4894 5503 5581 6021
Size: 327 609 78 440
Fragments arranged by size:
2984 609 537 440 390 327 273 188
118 78 58 19
SnaBI TAC'GTA
Cuts at: 0 3598 6021
Size: 3598 2423
SphI G_CATG'C
Cuts at: 0 495 6021
Size: 495 5526
SrfI GCCC'GGGC
Cuts at: 0 4561 6021
Size: 4561 1460
SspI AAT'ATT
Cuts at: 0 411 627 1104 1905 6021
Size: 411 216 477 801 4116
Fragments arranged by size:
4116 801 477 411 216
StuI AGG'CCT
Cuts at: 0 2329 2506 6021
Size: 2329 177 3515
Fragments arranged by size:
3515 2329 177
StyI C'CwwG_G
Cuts at: 0 126 254 1016 3890 4586 5594 6021
Size: 126 128 762 2874 696 1008 427
Fragments arranged by size:
2874 1008 762 696 427 128 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3301 6021
Size: 3301 2720
TatI w'GTAC_w
Cuts at: 0 422 1620 2372 6021
Size: 422 1198 752 3649
Fragments arranged by size:
3649 1198 752 422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2159 2191 3787 3819 6021
Size: 2159 32 1596 32 2202
Fragments arranged by size:
2202 2159 1596 32 32
XbaI T'CTAG_A
Cuts at: 0 3756 6021
Size: 3756 2265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 892 4173 4656 4782 5385 5544 6021
Size: 892 3281 483 126 603 159 477
Fragments arranged by size:
3281 892 603 483 477 159 126
XhoI C'TCGA_G
Cuts at: 0 19 3061 4567 4894 5503 6021
Size: 19 3042 1506 327 609 518
Fragments arranged by size:
3042 1506 609 518 327 19
XmaI C'CCGG_G
Cuts at: 0 4559 6021
Size: 4559 1462
XmnI GAAnn'nnTTC
Cuts at: 0 1055 1104 2651 2858 5159 6021
Size: 1055 49 1547 207 2301 862
Fragments arranged by size:
2301 1547 1055 862 207 49
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII KasI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PflMI PfoI PpiI PpuMI PsiI
PspOMI PspXI PstI PvuII SacI SalI SapI SbfI
SfcI SfiI SfoI SmaI SmlI SnaBI SphI SrfI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BglII BlpI BmgBI BsaWI BsiWI
BspEI BssHII BstBI BstZ17I CspCI DraIII FalI FseI
FspAI HpaI KpnI NdeI NotI NruI PacI PasI
PciI PmeI PmlI PshAI PsrI PvuI RsrII SacII
SanDI ScaI SexAI SgfI SgrAI SpeI SwaI Tth111I
ZraI