Restriction map for
pF1KB0049
(Linear) (Six-base) MAPSORT of: KIBB0049.seq Check: 5261 from: 1 to: 5469
KIBB0049.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 2714 4171 5469
Size: 2714 1457 1298
Fragments arranged by size:
2714 1457 1298
AclI AA'CG_TT
Cuts at: 0 666 2429 5469
Size: 666 1763 3040
Fragments arranged by size:
3040 1763 666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2775 3576 3812 4639 4766 5267 5397 5469
Size: 2775 801 236 827 127 501 130 72
Fragments arranged by size:
2775 827 801 501 236 130 127 72
AfeI AGC'GCT
Cuts at: 0 54 863 5469
Size: 54 809 4606
Fragments arranged by size:
4606 809 54
AflIII A'CryG_T
Cuts at: 0 1592 3321 3839 4592 5469
Size: 1592 1729 518 753 877
Fragments arranged by size:
1729 1592 877 753 518
AleI CACnn'nnGTG
Cuts at: 0 2329 3094 5469
Size: 2329 765 2375
Fragments arranged by size:
2375 2329 765
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 450 1837 2270 5469
Size: 450 1387 433 3199
Fragments arranged by size:
3199 1387 450 433
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1091 1123 3881 3913 4958 4990 5469
Size: 1091 32 2758 32 1045 32 479
Fragments arranged by size:
2758 1091 1045 479 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 2074 2190 2385 2777 5469
Size: 1386 688 116 195 392 2692
Fragments arranged by size:
2692 1386 688 392 195 116
ApaI G_GGCC'C
Cuts at: 0 3561 5469
Size: 3561 1908
ApaLI G'TGCA_C
Cuts at: 0 295 2536 5469
Size: 295 2241 2933
Fragments arranged by size:
2933 2241 295
ApoI r'AATT_y
Cuts at: 0 573 1924 1949 2893 3202 4251 5469
Size: 573 1351 25 944 309 1049 1218
Fragments arranged by size:
1351 1218 1049 944 573 309 25
AseI AT'TA_AT
Cuts at: 0 3392 5469
Size: 3392 2077
AvaI C'yCGr_G
Cuts at: 0 2541 2847 3107 5469
Size: 2541 306 260 2362
Fragments arranged by size:
2541 2362 306 260
BamHI G'GATC_C
Cuts at: 0 2450 4317 5469
Size: 2450 1867 1152
Fragments arranged by size:
2450 1867 1152
BanI G'GyrC_C
Cuts at: 0 4468 5437 5469
Size: 4468 969 32
Fragments arranged by size:
4468 969 32
BanII G_rGCy'C
Cuts at: 0 13 118 1325 2103 2462 3534 3561 5469
Size: 13 105 1207 778 359 1072 27 1908
Fragments arranged by size:
1908 1207 1072 778 359 105 27 13
BbeI G_GCGC'C
Cuts at: 0 5441 5469
Size: 5441 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1514 3692 4208 4726 5469
Size: 1514 2178 516 518 743
Fragments arranged by size:
2178 1514 743 518 516
BbvCI CC'TCA_GC
Cuts at: 0 1744 2378 5469
Size: 1744 634 3091
Fragments arranged by size:
3091 1744 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 463 497 2223 2257 5469
Size: 463 34 1726 34 3212
Fragments arranged by size:
3212 1726 463 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 280 912 4960 5469
Size: 280 632 4048 509
Fragments arranged by size:
4048 632 509 280
BclI T'GATC_A
Cuts at: 0 2207 3044 5289 5469
Size: 2207 837 2245 180
Fragments arranged by size:
2245 2207 837 180
BfrBI ATG'CAT
Cuts at: 0 1867 3602 5469
Size: 1867 1735 1867
Fragments arranged by size:
1867 1867 1735
BglI GCCn_nnn'nGGC
Cuts at: 0 39 2263 5469
Size: 39 2224 3206
Fragments arranged by size:
3206 2224 39
BglII A'GATC_T
Cuts at: 0 1458 3413 5469
Size: 1458 1955 2056
Fragments arranged by size:
2056 1955 1458
BlpI GC'TnA_GC
Cuts at: 0 12 117 895 5469
Size: 12 105 778 4574
Fragments arranged by size:
4574 778 105 12
Bme1580I G_kGCm'C
Cuts at: 0 242 299 397 2121 2540 3108 3561 5469
Size: 242 57 98 1724 419 568 453 1908
Fragments arranged by size:
1908 1724 568 453 419 242 98 57
BmgBI CAC'GTC
Cuts at: 0 2576 3840 5469
Size: 2576 1264 1629
Fragments arranged by size:
2576 1629 1264
BmrI ACTGGGnnnn_n'
Cuts at: 0 5398 5469
Size: 5398 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1074 1444 1447 3521 3664 4846 5469
Size: 69 1005 370 3 2074 143 1182 623
Fragments arranged by size:
2074 1182 1005 623 370 143 69 3
Bpu10I CC'TnA_GC
Cuts at: 0 282 1744 2378 5469
Size: 282 1462 634 3091
Fragments arranged by size:
3091 1462 634 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 1390 4717 5469
Size: 1390 3327 752
Fragments arranged by size:
3327 1390 752
BsaAI yAC'GTr
Cuts at: 0 930 4971 5469
Size: 930 4041 498
Fragments arranged by size:
4041 930 498
BsaBI GATnn'nnATC
Cuts at: 0 1012 3418 5469
Size: 1012 2406 2051
Fragments arranged by size:
2406 2051 1012
BsaHI Gr'CG_yC
Cuts at: 0 174 692 5438 5469
Size: 174 518 4746 31
Fragments arranged by size:
4746 518 174 31
BsaWI w'CCGG_w
Cuts at: 0 649 754 3163 5044 5469
Size: 649 105 2409 1881 425
Fragments arranged by size:
2409 1881 649 425 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 221 251 3829 3859 4955 4985 5290 5320
Size: 221 30 3578 30 1096 30 305 30
Cuts at: 5320 5469
Size: 149
Fragments arranged by size:
3578 1096 305 221 149 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 949 1348 2005 3524 3542 4222 5469
Size: 34 915 399 657 1519 18 680 1247
Fragments arranged by size:
1519 1247 915 680 657 399 34 18
BseYI C'CCAG_C
Cuts at: 0 2180 5353 5469
Size: 2180 3173 116
Fragments arranged by size:
3173 2180 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1372 2263 5469
Size: 734 638 891 3206
Fragments arranged by size:
3206 891 734 638
BsiEI CG_ry'CG
Cuts at: 0 191 2614 5469
Size: 191 2423 2855
Fragments arranged by size:
2855 2423 191
BsiHKAI G_wGCw'C
Cuts at: 0 13 118 299 326 375 510 1325 1606
Size: 13 105 181 27 49 135 815 281
Cuts at: 1606 2462 2540 3534 3570 4086 4403 5469
Size: 856 78 994 36 516 317 1066
Fragments arranged by size:
1066 994 856 815 516 317 281 181
135 105 78 49 36 27 13
BsiWI C'GTAC_G
Cuts at: 0 2398 5469
Size: 2398 3071
BsmI GAATG_Cn'
Cuts at: 0 766 1674 5469
Size: 766 908 3795
Fragments arranged by size:
3795 908 766
BsmBI CGTCTCn'nnnn_
Cuts at: 0 139 2582 5469
Size: 139 2443 2887
Fragments arranged by size:
2887 2443 139
Bsp1286I G_dGCh'C
Cuts at: 0 13 118 242 299 326 375 397 510
Size: 13 105 124 57 27 49 22 113
Cuts at: 510 1325 1606 2103 2121 2462 2540 3108 3534
Size: 815 281 497 18 341 78 568 426
Cuts at: 3534 3561 3570 4086 4403 5469
Size: 27 9 516 317 1066
Fragments arranged by size:
1066 815 568 516 497 426 341 317
281 124 113 105 78 57 49 27
27 22 18 13 9
BspEI T'CCGG_A
Cuts at: 0 5044 5469
Size: 5044 425
BspHI T'CATG_A
Cuts at: 0 4556 5469
Size: 4556 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 857 2427 3002 5469
Size: 857 1570 575 2467
Fragments arranged by size:
2467 1570 857 575
BsrBI CCG'CTC
Cuts at: 0 919 4344 5469
Size: 919 3425 1125
Fragments arranged by size:
3425 1125 919
BsrDI GCAATG_nn'
Cuts at: 0 207 2462 3315 3731 4365 5469
Size: 207 2255 853 416 634 1104
Fragments arranged by size:
2255 1104 853 634 416 207
BsrGI T'GTAC_A
Cuts at: 0 2588 3053 5469
Size: 2588 465 2416
Fragments arranged by size:
2588 2416 465
BssSI C'ACGA_G
Cuts at: 0 1324 5469
Size: 1324 4145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 800 1386 1927 5469
Size: 800 586 541 3542
Fragments arranged by size:
3542 800 586 541
BstBI TT'CG_AA
Cuts at: 0 3237 5469
Size: 3237 2232
BstEII G'GTnAC_C
Cuts at: 0 3313 5469
Size: 3313 2156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3855 4779 4870 5263 5469
Size: 3855 924 91 393 206
Fragments arranged by size:
3855 924 393 206 91
BstYI r'GATC_y
Cuts at: 0 579 1458 2450 2492 3413 4317 5469
Size: 579 879 992 42 921 904 1152
Fragments arranged by size:
1152 992 921 904 879 579 42
BstZ17I GTA'TAC
Cuts at: 0 2715 4172 5469
Size: 2715 1457 1297
Fragments arranged by size:
2715 1457 1297
Bsu36I CC'TnA_GG
Cuts at: 0 2930 5469
Size: 2930 2539
BtgI C'CryG_G
Cuts at: 0 2261 3173 4512 4575 4931 5058 5469
Size: 2261 912 1339 63 356 127 411
Fragments arranged by size:
2261 1339 912 411 356 127 63
BtsI GCAGTG_nn'
Cuts at: 0 297 573 722 2064 2075 2252 2324 2336
Size: 297 276 149 1342 11 177 72 12
Cuts at: 2336 4695 5469
Size: 2359 774
Fragments arranged by size:
2359 1342 774 297 276 177 149 72
12 11
DraIII CAC_nnn'GTG
Cuts at: 0 5388 5406 5469
Size: 5388 18 63
Fragments arranged by size:
5388 63 18
EaeI y'GGCC_r
Cuts at: 0 188 416 618 2264 2517 2611 3023 4578
Size: 188 228 202 1646 253 94 412 1555
Cuts at: 4578 4692 5061 5469
Size: 114 369 408
Fragments arranged by size:
1646 1555 412 408 369 253 228 202
188 114 94
EagI C'GGCC_G
Cuts at: 0 188 2611 5469
Size: 188 2423 2858
Fragments arranged by size:
2858 2423 188
EarI CTCTTCn'nnn_
Cuts at: 0 1214 1277 2316 4538 5469
Size: 1214 63 1039 2222 931
Fragments arranged by size:
2222 1214 1039 931 63
EcoICRI GAG'CTC
Cuts at: 0 11 116 1323 2460 3532 5469
Size: 11 105 1207 1137 1072 1937
Fragments arranged by size:
1937 1207 1137 1072 105 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1074 1444 1447 2775 3521 3576 3664
Size: 69 1005 370 3 1328 746 55 88
Cuts at: 3664 3812 4639 4766 4846 5267 5397 5469
Size: 148 827 127 80 421 130 72
Fragments arranged by size:
1328 1005 827 746 421 370 148 130
127 88 80 72 69 55 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 438 4016 5469
Size: 438 3578 1453
Fragments arranged by size:
3578 1453 438
EcoO109I rG'GnC_Cy
Cuts at: 0 834 1482 1785 3557 3558 4768 4874 4927
Size: 834 648 303 1772 1 1210 106 53
Cuts at: 4927 5469
Size: 542
Fragments arranged by size:
1772 1210 834 648 542 303 106 53
1
EcoRV GAT'ATC
Cuts at: 0 3585 5469
Size: 3585 1884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1120 3138 3648 3709 4077 4750 5469
Size: 1120 2018 510 61 368 673 719
Fragments arranged by size:
2018 1120 719 673 510 368 61
FspI TGC'GCA
Cuts at: 0 3307 5469
Size: 3307 2162
HaeII r_GCGC'y
Cuts at: 0 24 56 865 2190 5441 5469
Size: 24 32 809 1325 3251 28
Fragments arranged by size:
3251 1325 809 32 28 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 1389 1421 1614 1646 1962 1994
Size: 347 32 1010 32 193 32 316 32
Cuts at: 1994 2506 2538 2619 2651 3829 3861 3916 3948
Size: 512 32 81 32 1178 32 55 32
Cuts at: 3948 3993 4025 5227 5259 5469
Size: 45 32 1202 32 210
Fragments arranged by size:
1202 1178 1010 512 347 316 210 193
81 55 45 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1300 3459 5469
Size: 1300 2159 2010
Fragments arranged by size:
2159 2010 1300
KasI G'GCGC_C
Cuts at: 0 5437 5469
Size: 5437 32
MfeI C'AATT_G
Cuts at: 0 5285 5469
Size: 5285 184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 2990 4188 4403 5213 5244 5292 5469
Size: 287 2703 1198 215 810 31 48 177
Fragments arranged by size:
2703 1198 810 287 215 177 48 31
MscI TGG'CCA
Cuts at: 0 418 620 2266 2519 3025 5469
Size: 418 202 1646 253 506 2444
Fragments arranged by size:
2444 1646 506 418 253 202
MslI CAynn'nnrTG
Cuts at: 0 932 1997 2329 3094 4311 5469
Size: 932 1065 332 765 1217 1158
Fragments arranged by size:
1217 1158 1065 932 765 332
MspA1I CmG'CkG
Cuts at: 0 121 306 1149 1383 1431 1539 2527 3247
Size: 121 185 843 234 48 108 988 720
Cuts at: 3247 3347 5435 5469
Size: 100 2088 34
Fragments arranged by size:
2088 988 843 720 234 185 121 108
100 48 34
NarI GG'CG_CC
Cuts at: 0 5438 5469
Size: 5438 31
NcoI C'CATG_G
Cuts at: 0 2261 4512 4575 4931 5469
Size: 2261 2251 63 356 538
Fragments arranged by size:
2261 2251 538 356 63
NsiI A_TGCA'T
Cuts at: 0 1869 3604 5469
Size: 1869 1735 1865
Fragments arranged by size:
1869 1865 1735
NspI r_CATG'y
Cuts at: 0 1596 3325 3472 4596 5469
Size: 1596 1729 147 1124 873
Fragments arranged by size:
1729 1596 1124 873 147
PasI CC'CwG_GG
Cuts at: 0 3660 5469
Size: 3660 1809
PciI A'CATG_T
Cuts at: 0 1592 3321 4592 5469
Size: 1592 1729 1271 877
Fragments arranged by size:
1729 1592 1271 877
PfoI T'CCnGG_A
Cuts at: 0 1421 1463 2384 4794 5469
Size: 1421 42 921 2410 675
Fragments arranged by size:
2410 1421 921 675 42
PmlI CAC'GTG
Cuts at: 0 930 5469
Size: 930 4539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2860 2892 4488 4520 5469
Size: 2860 32 1596 32 949
Fragments arranged by size:
2860 1596 949 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 834 1785 4768 5469
Size: 834 951 2983 701
Fragments arranged by size:
2983 951 834 701
PspOMI G'GGCC_C
Cuts at: 0 3557 5469
Size: 3557 1912
PstI C_TGCA'G
Cuts at: 0 568 2331 2634 5469
Size: 568 1763 303 2835
Fragments arranged by size:
2835 1763 568 303
PvuII CAG'CTG
Cuts at: 0 1149 1431 2527 3247 5469
Size: 1149 282 1096 720 2222
Fragments arranged by size:
2222 1149 1096 720 282
SacI G_AGCT'C
Cuts at: 0 13 118 1325 2462 3534 5469
Size: 13 105 1207 1137 1072 1935
Fragments arranged by size:
1935 1207 1137 1072 105 13
SanDI GG'GwC_CC
Cuts at: 0 4768 5469
Size: 4768 701
SapI GCTCTTCn'nnn_
Cuts at: 0 1214 5469
Size: 1214 4255
SfcI C'TryA_G
Cuts at: 0 438 564 788 2327 2630 4833 5469
Size: 438 126 224 1539 303 2203 636
Fragments arranged by size:
2203 1539 636 438 303 224 126
SfoI GGC'GCC
Cuts at: 0 5439 5469
Size: 5439 30
SmlI C'TyrA_G
Cuts at: 0 2541 5469
Size: 2541 2928
SspI AAT'ATT
Cuts at: 0 1887 4995 5469
Size: 1887 3108 474
Fragments arranged by size:
3108 1887 474
StuI AGG'CCT
Cuts at: 0 2569 5469
Size: 2569 2900
StyI C'CwwG_G
Cuts at: 0 1238 2261 4512 4575 4931 5469
Size: 1238 1023 2251 63 356 538
Fragments arranged by size:
2251 1238 1023 538 356 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1327 1961 2083 2380 2638 3975 5178 5417
Size: 1327 634 122 297 258 1337 1203 239
Cuts at: 5417 5469
Size: 52
Fragments arranged by size:
1337 1327 1203 634 297 258 239 122
52
TatI w'GTAC_w
Cuts at: 0 2588 3053 3917 5469
Size: 2588 465 864 1552
Fragments arranged by size:
2588 1552 864 465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 221 253 278 310 1395 1427 4790 4822
Size: 221 32 25 32 1085 32 3363 32
Cuts at: 4822 4955 4987 5390 5422 5469
Size: 133 32 403 32 47
Fragments arranged by size:
3363 1085 403 221 133 47 32 32
32 32 32 32 25
XbaI T'CTAG_A
Cuts at: 0 2200 4977 5469
Size: 2200 2777 492
Fragments arranged by size:
2777 2200 492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 2259 5071 5469
Size: 270 1989 2812 398
Fragments arranged by size:
2812 1989 398 270
XhoI C'TCGA_G
Cuts at: 0 2541 5469
Size: 2541 2928
XmnI GAAnn'nnTTC
Cuts at: 0 3610 3631 4503 4552 4733 5040 5469
Size: 3610 21 872 49 181 307 429
Fragments arranged by size:
3610 872 429 307 181 49 21
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI FspI HaeII Hin4I HincII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NsiI NspI PasI
PciI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SfcI SfoI SmlI SspI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AscI AsiSI
AvrII BaeI BmtI BplI BpuEI BsrFI BssHII BtgZI
ClaI CspCI DraI DrdI EciI EcoRI FseI FspAI
HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI
NotI NruI PacI PflMI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI Tth111I XmaI ZraI