Restriction map for
pF1KB0051
(Linear) (Six-base) MAPSORT of: KIBB0051.seq Check: 7364 from: 1 to: 5373
KIBB0051.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 5373
Size: 204 5169
AatII G_ACGT'C
Cuts at: 0 2783 5373
Size: 2783 2590
AccI GT'mk_AC
Cuts at: 0 3713 5373
Size: 3713 1660
Acc65I G'GTAC_C
Cuts at: 0 2174 5042 5373
Size: 2174 2868 331
Fragments arranged by size:
2868 2174 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1135 1357 3436 4533 5373
Size: 132 1003 222 2079 1097 840
Fragments arranged by size:
2079 1097 1003 840 222 132
AflIII A'CryG_T
Cuts at: 0 200 269 410 5066 5117 5373
Size: 200 69 141 4656 51 256
Fragments arranged by size:
4656 256 200 141 69 51
AhdI GACnn_n'nnGTC
Cuts at: 0 411 641 4563 5373
Size: 411 230 3922 810
Fragments arranged by size:
3922 810 411 230
AleI CACnn'nnGTG
Cuts at: 0 1576 2377 4354 5373
Size: 1576 801 1977 1019
Fragments arranged by size:
1977 1576 1019 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 314 3592 5275 5373
Size: 314 3278 1683 98
Fragments arranged by size:
3278 1683 314 98
AlwNI CAG_nnn'CTG
Cuts at: 0 1959 2019 2451 4986 5373
Size: 1959 60 432 2535 387
Fragments arranged by size:
2535 1959 432 387 60
ApaI G_GGCC'C
Cuts at: 0 527 3167 3260 5373
Size: 527 2640 93 2113
Fragments arranged by size:
2640 2113 527 93
ApaLI G'TGCA_C
Cuts at: 0 5235 5373
Size: 5235 138
AvaI C'yCGr_G
Cuts at: 0 50 1308 1816 2104 2670 3102 3614 4906
Size: 50 1258 508 288 566 432 512 1292
Cuts at: 4906 4952 5373
Size: 46 421
Fragments arranged by size:
1292 1258 566 512 508 432 421 288
50 46
BanI G'GyrC_C
Cuts at: 0 298 1468 2174 2490 4331 4852 5042 5102
Size: 298 1170 706 316 1841 521 190 60
Cuts at: 5102 5373
Size: 271
Fragments arranged by size:
1841 1170 706 521 316 298 271 190
60
BanII G_rGCy'C
Cuts at: 0 527 1234 1367 1703 1751 2105 2677 3167
Size: 527 707 133 336 48 354 572 490
Cuts at: 3167 3260 3413 4283 4907 4979 5373
Size: 93 153 870 624 72 394
Fragments arranged by size:
870 707 624 572 527 490 394 354
336 153 133 93 72 48
BbeI G_GCGC'C
Cuts at: 0 4335 5106 5373
Size: 4335 771 267
Fragments arranged by size:
4335 771 267
BbsI GAAGACnn'nnnn_
Cuts at: 0 651 904 1644 4164 4477 5373
Size: 651 253 740 2520 313 896
Fragments arranged by size:
2520 896 740 651 313 253
BbvCI CC'TCA_GC
Cuts at: 0 1033 3168 4604 5373
Size: 1033 2135 1436 769
Fragments arranged by size:
2135 1436 1033 769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2464 2498 5373
Size: 2464 34 2875
Fragments arranged by size:
2875 2464 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 256 3973 5373
Size: 256 3717 1400
Fragments arranged by size:
3717 1400 256
BfrBI ATG'CAT
Cuts at: 0 2627 3791 5286 5373
Size: 2627 1164 1495 87
Fragments arranged by size:
2627 1495 1164 87
BglI GCCn_nnn'nGGC
Cuts at: 0 1432 2965 5373
Size: 1432 1533 2408
Fragments arranged by size:
2408 1533 1432
BglII A'GATC_T
Cuts at: 0 3722 5373
Size: 3722 1651
BlpI GC'TnA_GC
Cuts at: 0 1361 1868 3580 3655 4282 4447 5373
Size: 1361 507 1712 75 627 165 926
Fragments arranged by size:
1712 1361 926 627 507 165 75
Bme1580I G_kGCm'C
Cuts at: 0 527 787 1628 2495 3167 3209 3260 3644
Size: 527 260 841 867 672 42 51 384
Cuts at: 3644 5239 5373
Size: 1595 134
Fragments arranged by size:
1595 867 841 672 527 384 260 134
51 42
BmgBI CAC'GTC
Cuts at: 0 2186 3159 3456 3576 4716 5069 5373
Size: 2186 973 297 120 1140 353 304
Fragments arranged by size:
2186 1140 973 353 304 297 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 1890 5373
Size: 1890 3483
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 2511 3417 4944 5373
Size: 558 1953 906 1527 429
Fragments arranged by size:
1953 1527 906 558 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 1650 1927 1936 1981 2026 2049 2059
Size: 1214 436 277 9 45 45 23 10
Cuts at: 2059 2452 2481 2791 3754 3902 4413 4459 4525
Size: 393 29 310 963 148 511 46 66
Cuts at: 4525 4563 4645 5077 5328 5373
Size: 38 82 432 251 45
Fragments arranged by size:
1214 963 511 436 432 393 310 277
251 148 82 66 46 45 45 45
38 29 23 10 9
Bpu10I CC'TnA_GC
Cuts at: 0 1024 1033 2440 2872 3168 4604 5373
Size: 1024 9 1407 432 296 1436 769
Fragments arranged by size:
1436 1407 1024 769 432 296 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 951 1011 2209 5194 5373
Size: 778 173 60 1198 2985 179
Fragments arranged by size:
2985 1198 778 179 173 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 691 1012 1366 2531 3916 4501 5373
Size: 628 63 321 354 1165 1385 585 872
Fragments arranged by size:
1385 1165 872 628 585 354 321 63
BsaAI yAC'GTr
Cuts at: 0 531 4296 5373
Size: 531 3765 1077
Fragments arranged by size:
3765 1077 531
BsaHI Gr'CG_yC
Cuts at: 0 136 1861 2687 2780 3008 3328 4332 5103
Size: 136 1725 826 93 228 320 1004 771
Cuts at: 5103 5373
Size: 270
Fragments arranged by size:
1725 1004 826 771 320 270 228 136
93
BsaWI w'CCGG_w
Cuts at: 0 868 1409 1655 1802 2159 2787 3976 4004
Size: 868 541 246 147 357 628 1189 28
Cuts at: 4004 5373
Size: 1369
Fragments arranged by size:
1369 1189 868 628 541 357 246 147
28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1954 1984 4395 4425 4522 4552 5373
Size: 1954 30 2411 30 97 30 821
Fragments arranged by size:
2411 1954 821 97 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 931 1333 1396 1654 2002 2104 2107
Size: 250 681 402 63 258 348 102 3
Cuts at: 2107 2110 2113 2719 2737 2740 2743 2746 2749
Size: 3 3 606 18 3 3 3 3
Cuts at: 2749 2752 2755 2758 2761 2770 2773 2776 3211
Size: 3 3 3 3 9 3 3 435
Cuts at: 3211 3796 4390 4566 4578 4806 5373
Size: 585 594 176 12 228 567
Fragments arranged by size:
681 606 594 585 567 435 402 348
258 250 228 176 102 63 18 12
9 3 3 3 3 3 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 519 748 1750 1892 3990 4136 4514 4979
Size: 519 229 1002 142 2098 146 378 465
Cuts at: 4979 5075 5373
Size: 96 298
Fragments arranged by size:
2098 1002 519 465 378 298 229 146
142 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3173 4116 5032 5373
Size: 3173 943 916 341
Fragments arranged by size:
3173 943 916 341
BsiEI CG_ry'CG
Cuts at: 0 2478 2811 2829 3761 5027 5373
Size: 2478 333 18 932 1266 346
Fragments arranged by size:
2478 1266 932 346 333 18
BsiHKAI G_wGCw'C
Cuts at: 0 512 1367 1646 2795 3581 4283 5159 5239
Size: 512 855 279 1149 786 702 876 80
Cuts at: 5239 5373
Size: 134
Fragments arranged by size:
1149 876 855 786 702 512 279 134
80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 127 1852 2192 2347 5373
Size: 127 1725 340 155 3026
Fragments arranged by size:
3026 1725 340 155 127
Bsp1286I G_dGCh'C
Cuts at: 0 512 527 787 1234 1367 1628 1646 1703
Size: 512 15 260 447 133 261 18 57
Cuts at: 1703 1751 2105 2495 2677 2795 3167 3209 3260
Size: 48 354 390 182 118 372 42 51
Cuts at: 3260 3413 3581 3644 4283 4907 4979 5159 5239
Size: 153 168 63 639 624 72 180 80
Cuts at: 5239 5373
Size: 134
Fragments arranged by size:
639 624 512 447 390 372 354 261
260 182 180 168 153 134 133 118
80 72 63 57 51 48 42 18
15
BspEI T'CCGG_A
Cuts at: 0 868 1409 1655 2159 2787 3976 5373
Size: 868 541 246 504 628 1189 1397
Fragments arranged by size:
1397 1189 868 628 541 504 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 2009 2207 2330 2888 3713 4493 5054
Size: 204 1805 198 123 558 825 780 561
Cuts at: 5054 5373
Size: 319
Fragments arranged by size:
1805 825 780 561 558 319 204 198
123
BsrBI CCG'CTC
Cuts at: 0 759 1983 2130 3101 4596 5148 5373
Size: 759 1224 147 971 1495 552 225
Fragments arranged by size:
1495 1224 971 759 552 225 147
BsrDI GCAATG_nn'
Cuts at: 0 1074 5373
Size: 1074 4299
BsrFI r'CCGG_y
Cuts at: 0 301 2530 2828 4140 4328 5373
Size: 301 2229 298 1312 188 1045
Fragments arranged by size:
2229 1312 1045 301 298 188
BsrGI T'GTAC_A
Cuts at: 0 5120 5373
Size: 5120 253
BssSI C'ACGA_G
Cuts at: 0 1288 5373
Size: 1288 4085
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 3788 4485 5205 5373
Size: 516 3272 697 720 168
Fragments arranged by size:
3272 720 697 516 168
BstEII G'GTnAC_C
Cuts at: 0 1125 1689 2526 3477 4071 5373
Size: 1125 564 837 951 594 1302
Fragments arranged by size:
1302 1125 951 837 594 564
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 1162 1218 1413 2247 5373
Size: 612 550 56 195 834 3126
Fragments arranged by size:
3126 834 612 550 195 56
BstYI r'GATC_y
Cuts at: 0 1058 3722 5009 5373
Size: 1058 2664 1287 364
Fragments arranged by size:
2664 1287 1058 364
Bsu36I CC'TnA_GG
Cuts at: 0 185 3589 4864 5373
Size: 185 3404 1275 509
Fragments arranged by size:
3404 1275 509 185
BtgI C'CryG_G
Cuts at: 0 422 611 710 770 2384 3083 3761 3774
Size: 422 189 99 60 1614 699 678 13
Cuts at: 3774 4569 5141 5247 5373
Size: 795 572 106 126
Fragments arranged by size:
1614 795 699 678 572 422 189 126
106 99 60 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3450 5373
Size: 3450 1923
BtsI GCAGTG_nn'
Cuts at: 0 514 1644 3209 3476 3690 4236 5168 5373
Size: 514 1130 1565 267 214 546 932 205
Fragments arranged by size:
1565 1130 932 546 514 267 214 205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 90 125 5373
Size: 90 35 5248
Fragments arranged by size:
5248 90 35
DraIII CAC_nnn'GTG
Cuts at: 0 2910 5373
Size: 2910 2463
DrdI GACnn_nn'nnGTC
Cuts at: 0 1723 3709 3930 5331 5373
Size: 1723 1986 221 1401 42
Fragments arranged by size:
1986 1723 1401 221 42
EaeI y'GGCC_r
Cuts at: 0 2 617 1433 1448 2381 2808 2957 3534
Size: 2 615 816 15 933 427 149 577
Cuts at: 3534 3758 4655 4799 4888 5165 5318 5373
Size: 224 897 144 89 277 153 55
Fragments arranged by size:
933 897 816 615 577 427 277 224
153 149 144 89 55 15 2
EagI C'GGCC_G
Cuts at: 0 2808 3758 5373
Size: 2808 950 1615
Fragments arranged by size:
2808 1615 950
EarI CTCTTCn'nnn_
Cuts at: 0 382 2618 2978 3113 3978 5373
Size: 382 2236 360 135 865 1395
Fragments arranged by size:
2236 1395 865 382 360 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 837 1410 2549 3001 3274 5373
Size: 25 812 573 1139 452 273 2099
Fragments arranged by size:
2099 1139 812 573 452 273 25
EcoICRI GAG'CTC
Cuts at: 0 1365 4281 5373
Size: 1365 2916 1092
Fragments arranged by size:
2916 1365 1092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1135 1214 1357 1650 1927 1936 1981
Size: 132 1003 79 143 293 277 9 45
Cuts at: 1981 2026 2049 2059 2452 2481 2791 3436 3754
Size: 45 23 10 393 29 310 645 318
Cuts at: 3754 3902 4413 4459 4525 4533 4563 4645 5077
Size: 148 511 46 66 8 30 82 432
Cuts at: 5077 5328 5373
Size: 251 45
Fragments arranged by size:
1003 645 511 432 393 318 310 293
277 251 148 143 132 82 79 66
46 45 45 45 30 29 23 10
9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 796 835 1519 2227 3568 3811 4456 4459
Size: 796 39 684 708 1341 243 645 3
Cuts at: 4459 5260 5373
Size: 801 113
Fragments arranged by size:
1341 801 796 708 684 645 243 113
39 3
EcoO109I rG'GnC_Cy
Cuts at: 0 372 523 524 537 579 1086 1140 1198
Size: 372 151 1 13 42 507 54 58
Cuts at: 1198 1524 2196 2766 2814 2858 2988 3163 3164
Size: 326 672 570 48 44 130 175 1
Cuts at: 3164 3257 3433 3593 3682 3797 4397 4773 4868
Size: 93 176 160 89 115 600 376 95
Cuts at: 4868 5373
Size: 505
Fragments arranged by size:
672 600 570 507 505 376 372 326
176 175 160 151 130 115 95 93
89 58 54 48 44 42 13 1
1
HaeII r_GCGC'y
Cuts at: 0 687 2149 4329 4335 4586 4986 5088 5106
Size: 687 1462 2180 6 251 400 102 18
Cuts at: 5106 5373
Size: 267
Fragments arranged by size:
2180 1462 687 400 267 251 102 18
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 397 429 627 659 2986 3018
Size: 364 32 1 32 198 32 2327 32
Cuts at: 3018 4076 4108 4395 4427 4522 4549 4554 4581
Size: 1058 32 287 32 95 27 5 27
Cuts at: 4581 4773 4805 5373
Size: 192 32 568
Fragments arranged by size:
2327 1058 568 364 287 198 192 95
32 32 32 32 32 32 32 27
27 5 1
HincII GTy'rAC
Cuts at: 0 114 932 1512 3183 5373
Size: 114 818 580 1671 2190
Fragments arranged by size:
2190 1671 818 580 114
HindIII A'AGCT_T
Cuts at: 0 144 5373
Size: 144 5229
HpaI GTT'AAC
Cuts at: 0 114 5373
Size: 114 5259
KasI G'GCGC_C
Cuts at: 0 4331 5102 5373
Size: 4331 771 271
Fragments arranged by size:
4331 771 271
KpnI G_GTAC'C
Cuts at: 0 2178 5046 5373
Size: 2178 2868 327
Fragments arranged by size:
2868 2178 327
MluI A'CGCG_T
Cuts at: 0 200 5373
Size: 200 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2708 5373
Size: 2708 2665
MscI TGG'CCA
Cuts at: 0 1450 3536 4801 5167 5373
Size: 1450 2086 1265 366 206
Fragments arranged by size:
2086 1450 1265 366 206
MslI CAynn'nnrTG
Cuts at: 0 85 319 625 1411 1576 1780 1924 2245
Size: 85 234 306 786 165 204 144 321
Cuts at: 2245 2377 2626 3469 4354 5373
Size: 132 249 843 885 1019
Fragments arranged by size:
1019 885 843 786 321 306 249 234
204 165 144 132 85
MspA1I CmG'CkG
Cuts at: 0 1064 1530 1867 2016 3872 4518 4647 5249
Size: 1064 466 337 149 1856 646 129 602
Cuts at: 5249 5373
Size: 124
Fragments arranged by size:
1856 1064 646 602 466 337 149 129
124
NaeI GCC'GGC
Cuts at: 0 303 4142 4330 5373
Size: 303 3839 188 1043
Fragments arranged by size:
3839 1043 303 188
NarI GG'CG_CC
Cuts at: 0 4332 5103 5373
Size: 4332 771 270
Fragments arranged by size:
4332 771 270
NcoI C'CATG_G
Cuts at: 0 4569 5373
Size: 4569 804
NgoMIV G'CCGG_C
Cuts at: 0 301 4140 4328 5373
Size: 301 3839 188 1045
Fragments arranged by size:
3839 1045 301 188
NsiI A_TGCA'T
Cuts at: 0 2629 3793 5288 5373
Size: 2629 1164 1495 85
Fragments arranged by size:
2629 1495 1164 85
NspI r_CATG'y
Cuts at: 0 253 414 2627 3786 5121 5286 5373
Size: 253 161 2213 1159 1335 165 87
Fragments arranged by size:
2213 1335 1159 253 165 161 87
PasI CC'CwG_GG
Cuts at: 0 831 1155 4315 5373
Size: 831 324 3160 1058
Fragments arranged by size:
3160 1058 831 324
PciI A'CATG_T
Cuts at: 0 410 5117 5373
Size: 410 4707 256
Fragments arranged by size:
4707 410 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1959 5373
Size: 1959 3414
PfoI T'CCnGG_A
Cuts at: 0 575 944 1952 2393 3678 4343 4526 4769
Size: 575 369 1008 441 1285 665 183 243
Cuts at: 4769 5003 5054 5373
Size: 234 51 319
Fragments arranged by size:
1285 1008 665 575 441 369 319 243
234 183 51
PmlI CAC'GTG
Cuts at: 0 4296 5373
Size: 4296 1077
PpuMI rG'GwC_Cy
Cuts at: 0 372 537 579 1086 1140 1198 1524 2196
Size: 372 165 42 507 54 58 326 672
Cuts at: 2196 2766 2814 3682 3797 4773 4868 5373
Size: 570 48 868 115 976 95 505
Fragments arranged by size:
976 868 672 570 507 505 372 326
165 115 95 58 54 48 42
PspOMI G'GGCC_C
Cuts at: 0 523 3163 3256 5373
Size: 523 2640 93 2117
Fragments arranged by size:
2640 2117 523 93
PstI C_TGCA'G
Cuts at: 0 248 1002 2051 2487 3326 4460 5264 5373
Size: 248 754 1049 436 839 1134 804 109
Fragments arranged by size:
1134 1049 839 804 754 436 248 109
PvuI CG_AT'CG
Cuts at: 0 5027 5373
Size: 5027 346
PvuII CAG'CTG
Cuts at: 0 1867 5373
Size: 1867 3506
SacI G_AGCT'C
Cuts at: 0 1367 4283 5373
Size: 1367 2916 1090
Fragments arranged by size:
2916 1367 1090
SacII CC_GC'GG
Cuts at: 0 5250 5373
Size: 5250 123
SanDI GG'GwC_CC
Cuts at: 0 2814 3682 4773 5373
Size: 2814 868 1091 600
Fragments arranged by size:
2814 1091 868 600
SapI GCTCTTCn'nnn_
Cuts at: 0 3113 5373
Size: 3113 2260
SbfI CC_TGCA'GG
Cuts at: 0 3326 4460 5373
Size: 3326 1134 913
Fragments arranged by size:
3326 1134 913
SfcI C'TryA_G
Cuts at: 0 244 998 2047 2483 3322 4456 5260 5373
Size: 244 754 1049 436 839 1134 804 113
Fragments arranged by size:
1134 1049 839 804 754 436 244 113
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2965 5373
Size: 2965 2408
SfoI GGC'GCC
Cuts at: 0 4333 5104 5373
Size: 4333 771 269
Fragments arranged by size:
4333 771 269
SgrAI Cr'CCGG_yG
Cuts at: 0 4328 5373
Size: 4328 1045
SmaI CCC'GGG
Cuts at: 0 52 1310 4954 5373
Size: 52 1258 3644 419
Fragments arranged by size:
3644 1258 419 52
SmlI C'TyrA_G
Cuts at: 0 793 966 990 2224 2670 5209 5373
Size: 793 173 24 1234 446 2539 164
Fragments arranged by size:
2539 1234 793 446 173 164 24
SphI G_CATG'C
Cuts at: 0 253 3786 5373
Size: 253 3533 1587
Fragments arranged by size:
3533 1587 253
SrfI GCCC'GGGC
Cuts at: 0 4954 5373
Size: 4954 419
StuI AGG'CCT
Cuts at: 0 3218 3397 5373
Size: 3218 179 1976
Fragments arranged by size:
3218 1976 179
StyI C'CwwG_G
Cuts at: 0 128 779 1472 1694 4154 4569 5373
Size: 128 651 693 222 2460 415 804
Fragments arranged by size:
2460 804 693 651 415 222 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 527 691 1432 2363 2431 3940 4004 4150
Size: 527 164 741 931 68 1509 64 146
Cuts at: 4150 5232 5373
Size: 1082 141
Fragments arranged by size:
1509 1082 931 741 527 164 146 141
68 64
TatI w'GTAC_w
Cuts at: 0 3497 4700 5120 5183 5373
Size: 3497 1203 420 63 190
Fragments arranged by size:
3497 1203 420 190 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 2188 2220 4414 4446 5373
Size: 254 32 1902 32 2194 32 927
Fragments arranged by size:
2194 1902 927 254 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2779 3483 5373
Size: 2779 704 1890
Fragments arranged by size:
2779 1890 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1218 2001 2382 5088 5175 5373
Size: 1218 783 381 2706 87 198
Fragments arranged by size:
2706 1218 783 381 198 87
XhoI C'TCGA_G
Cuts at: 0 2670 5373
Size: 2670 2703
XmaI C'CCGG_G
Cuts at: 0 50 1308 4952 5373
Size: 50 1258 3644 421
Fragments arranged by size:
3644 1258 421 50
XmnI GAAnn'nnTTC
Cuts at: 0 2944 3122 5373
Size: 2944 178 2251
Fragments arranged by size:
2944 2251 178
ZraI GAC'GTC
Cuts at: 0 2781 5373
Size: 2781 2592
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuI
PvuII SacI SacII SanDI SapI SbfI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BclI BmtI BsaBI BsiWI
BsmI BspHI BssHII BstBI BstZ17I ClaI DraI EcoRI
EcoRV FalI FseI FspI FspAI MfeI NdeI NheI
NotI NruI PacI PmeI PpiI PshAI PsiI PspXI
PsrI RsrII SalI ScaI SexAI SgfI SnaBI SpeI
SspI SwaI XbaI