Restriction map for
pF1KB0054
(Linear) (Six-base) MAPSORT of: KIBB0054.seq Check: 6676 from: 1 to: 3108
KIBB0054.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 773 2211 2498 3108
Size: 773 1438 287 610
Fragments arranged by size:
1438 773 610 287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 920 1509 2048 2134 2584 2959 3108
Size: 403 517 589 539 86 450 375 149
Fragments arranged by size:
589 539 517 450 403 375 149 86
AflII C'TTAA_G
Cuts at: 0 1342 3108
Size: 1342 1766
AflIII A'CryG_T
Cuts at: 0 1225 2700 3108
Size: 1225 1475 408
Fragments arranged by size:
1475 1225 408
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2420 2452 3108
Size: 2420 32 656
Fragments arranged by size:
2420 656 32
ApaLI G'TGCA_C
Cuts at: 0 751 3108
Size: 751 2357
ApoI r'AATT_y
Cuts at: 0 249 587 1065 1723 1865 3108
Size: 249 338 478 658 142 1243
Fragments arranged by size:
1243 658 478 338 249 142
AseI AT'TA_AT
Cuts at: 0 1241 1574 2366 3108
Size: 1241 333 792 742
Fragments arranged by size:
1241 792 742 333
AvaI C'yCGr_G
Cuts at: 0 2828 3108
Size: 2828 280
BanII G_rGCy'C
Cuts at: 0 1753 3108
Size: 1753 1355
BbsI GAAGACnn'nnnn_
Cuts at: 0 1029 2906 3108
Size: 1029 1877 202
Fragments arranged by size:
1877 1029 202
BciVI GTATCCnnnnn_n'
Cuts at: 0 1954 3108
Size: 1954 1154
BclI T'GATC_A
Cuts at: 0 2007 2088 3108
Size: 2007 81 1020
Fragments arranged by size:
2007 1020 81
BfrBI ATG'CAT
Cuts at: 0 313 1116 3108
Size: 313 803 1992
Fragments arranged by size:
1992 803 313
BglII A'GATC_T
Cuts at: 0 187 795 3108
Size: 187 608 2313
Fragments arranged by size:
2313 608 187
BlpI GC'TnA_GC
Cuts at: 0 2036 3108
Size: 2036 1072
Bme1580I G_kGCm'C
Cuts at: 0 723 755 2153 2759 3108
Size: 723 32 1398 606 349
Fragments arranged by size:
1398 723 606 349 32
BmgBI CAC'GTC
Cuts at: 0 2703 3108
Size: 2703 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 2447 3108
Size: 132 2315 661
Fragments arranged by size:
2315 661 132
Bpu10I CC'TnA_GC
Cuts at: 0 1747 2856 3108
Size: 1747 1109 252
Fragments arranged by size:
1747 1109 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2887 3108
Size: 2887 221
BsaHI Gr'CG_yC
Cuts at: 0 4 3108
Size: 4 3104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2420 2450 3108
Size: 2420 30 658
Fragments arranged by size:
2420 658 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2267 2446 3108
Size: 2267 179 662
Fragments arranged by size:
2267 662 179
BseYI C'CCAG_C
Cuts at: 0 1956 2843 2916 3108
Size: 1956 887 73 192
Fragments arranged by size:
1956 887 192 73
BsiHKAI G_wGCw'C
Cuts at: 0 755 3108
Size: 755 2353
BsmBI CGTCTCn'nnnn_
Cuts at: 0 325 414 517 3108
Size: 325 89 103 2591
Fragments arranged by size:
2591 325 103 89
Bsp1286I G_dGCh'C
Cuts at: 0 723 755 1753 2153 2759 3108
Size: 723 32 998 400 606 349
Fragments arranged by size:
998 723 606 400 349 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 473 3108
Size: 473 2635
BsrBI CCG'CTC
Cuts at: 0 3021 3108
Size: 3021 87
BsrDI GCAATG_nn'
Cuts at: 0 313 1380 1836 1955 2391 3108
Size: 313 1067 456 119 436 717
Fragments arranged by size:
1067 717 456 436 313 119
BsrGI T'GTAC_A
Cuts at: 0 263 3108
Size: 263 2845
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1559 3108
Size: 1559 1549
BstBI TT'CG_AA
Cuts at: 0 247 1134 3108
Size: 247 887 1974
Fragments arranged by size:
1974 887 247
BstEII G'GTnAC_C
Cuts at: 0 2477 3108
Size: 2477 631
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1861 2769 3108
Size: 1861 908 339
Fragments arranged by size:
1861 908 339
BstYI r'GATC_y
Cuts at: 0 187 795 3108
Size: 187 608 2313
Fragments arranged by size:
2313 608 187
BstZ17I GTA'TAC
Cuts at: 0 774 2499 3108
Size: 774 1725 609
Fragments arranged by size:
1725 774 609
BtgI C'CryG_G
Cuts at: 0 11 2795 3108
Size: 11 2784 313
Fragments arranged by size:
2784 313 11
BtsI GCAGTG_nn'
Cuts at: 0 96 456 752 897 1222 3108
Size: 96 360 296 145 325 1886
Fragments arranged by size:
1886 360 325 296 145 96
ClaI AT'CG_AT
Cuts at: 0 338 2132 3108
Size: 338 1794 976
Fragments arranged by size:
1794 976 338
DraIII CAC_nnn'GTG
Cuts at: 0 747 3108
Size: 747 2361
EaeI y'GGCC_r
Cuts at: 0 23 3108
Size: 23 3085
EarI CTCTTCn'nnn_
Cuts at: 0 422 995 3108
Size: 422 573 2113
Fragments arranged by size:
2113 573 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 403 920 1509 2048 2134 2447 2584
Size: 132 271 517 589 539 86 313 137
Cuts at: 2584 2959 3108
Size: 375 149
Fragments arranged by size:
589 539 517 375 313 271 149 137
132 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2249 3108
Size: 2249 859
EcoO109I rG'GnC_Cy
Cuts at: 0 2789 3108
Size: 2789 319
EcoRI G'AATT_C
Cuts at: 0 1865 3108
Size: 1865 1243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2218 3108
Size: 2218 890
HaeII r_GCGC'y
Cuts at: 0 1907 3108
Size: 1907 1201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 723 755 1147 1179 2125 2157 2226 2258
Size: 723 32 392 32 946 32 69 32
Cuts at: 2258 3108
Size: 850
Fragments arranged by size:
946 850 723 392 69 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 239 401 3108
Size: 239 162 2707
Fragments arranged by size:
2707 239 162
MfeI C'AATT_G
Cuts at: 0 293 518 3108
Size: 293 225 2590
Fragments arranged by size:
2590 293 225
MluI A'CGCG_T
Cuts at: 0 1225 3108
Size: 1225 1883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1682 3108
Size: 1682 1426
MslI CAynn'nnrTG
Cuts at: 0 1859 2156 2767 2908 3108
Size: 1859 297 611 141 200
Fragments arranged by size:
1859 611 297 200 141
MspA1I CmG'CkG
Cuts at: 0 88 376 1843 2026 2718 3055 3108
Size: 88 288 1467 183 692 337 53
Fragments arranged by size:
1467 692 337 288 183 88 53
NcoI C'CATG_G
Cuts at: 0 2795 3108
Size: 2795 313
NdeI CA'TA_TG
Cuts at: 0 1596 3108
Size: 1596 1512
NsiI A_TGCA'T
Cuts at: 0 315 1118 3108
Size: 315 803 1990
Fragments arranged by size:
1990 803 315
NspI r_CATG'y
Cuts at: 0 105 313 506 1710 3108
Size: 105 208 193 1204 1398
Fragments arranged by size:
1398 1204 208 193 105
PasI CC'CwG_GG
Cuts at: 0 477 1966 3108
Size: 477 1489 1142
Fragments arranged by size:
1489 1142 477
PflMI CCAn_nnn'nTGG
Cuts at: 0 1759 1831 3108
Size: 1759 72 1277
Fragments arranged by size:
1759 1277 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2420 2452 3108
Size: 2420 32 656
Fragments arranged by size:
2420 656 32
PpuMI rG'GwC_Cy
Cuts at: 0 2789 3108
Size: 2789 319
PsiI TTA'TAA
Cuts at: 0 917 1121 3108
Size: 917 204 1987
Fragments arranged by size:
1987 917 204
PstI C_TGCA'G
Cuts at: 0 1085 1103 1391 3108
Size: 1085 18 288 1717
Fragments arranged by size:
1717 1085 288 18
PvuII CAG'CTG
Cuts at: 0 88 1843 2026 2718 3055 3108
Size: 88 1755 183 692 337 53
Fragments arranged by size:
1755 692 337 183 88 53
SanDI GG'GwC_CC
Cuts at: 0 2789 3108
Size: 2789 319
SapI GCTCTTCn'nnn_
Cuts at: 0 422 3108
Size: 422 2686
SfcI C'TryA_G
Cuts at: 0 813 1081 1099 1387 3031 3108
Size: 813 268 18 288 1644 77
Fragments arranged by size:
1644 813 288 268 77 18
SmlI C'TyrA_G
Cuts at: 0 1342 2828 2866 3108
Size: 1342 1486 38 242
Fragments arranged by size:
1486 1342 242 38
SphI G_CATG'C
Cuts at: 0 105 1710 3108
Size: 105 1605 1398
Fragments arranged by size:
1605 1398 105
SspI AAT'ATT
Cuts at: 0 1302 2625 3108
Size: 1302 1323 483
Fragments arranged by size:
1323 1302 483
StuI AGG'CCT
Cuts at: 0 788 3108
Size: 788 2320
StyI C'CwwG_G
Cuts at: 0 365 497 2795 3108
Size: 365 132 2298 313
Fragments arranged by size:
2298 365 313 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 958 3108
Size: 958 2150
TatI w'GTAC_w
Cuts at: 0 263 396 422 437 1077 2583 3108
Size: 263 133 26 15 640 1506 525
Fragments arranged by size:
1506 640 525 263 133 26 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1962 1994 3108
Size: 1962 32 1114
Fragments arranged by size:
1962 1114 32
XbaI T'CTAG_A
Cuts at: 0 2928 3108
Size: 2928 180
XhoI C'TCGA_G
Cuts at: 0 2828 3108
Size: 2828 280
Enzymes that do cut:
AccI AcuI AflII AflIII AloI ApaLI ApoI AseI
AvaI BanII BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtsI
ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HindIII MfeI MluI MmeI MslI
MspA1I NcoI NdeI NsiI NspI PasI PflMI PpiI
PpuMI PsiI PstI PvuII SanDI SapI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AlwNI ApaI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BglI BmrI BmtI BplI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrFI BssHII BssSI Bsu36I BtgZI CspCI
DraI DrdI EagI EciI EcoICRI EcoRV FseI FspI
FspAI HincII HpaI KasI KpnI MscI NaeI NarI
NgoMIV NheI NotI NruI PacI PciI PfoI PmeI
PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XcmI
XmaI XmnI ZraI