Restriction map for
pF1KSDB0055
(Linear) (Six-base) MAPSORT of: KIBB0055.seq Check: 6350 from: 1 to: 3996
KIBB0055.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AatII G_ACGT'C
Cuts at: 0 275 1538 3996
Size: 275 1263 2458
Fragments arranged by size:
2458 1263 275
AccI GT'mk_AC
Cuts at: 0 131 2414 3287 3707 3996
Size: 131 2283 873 420 289
Fragments arranged by size:
2283 873 420 289 131
Acc65I G'GTAC_C
Cuts at: 0 3468 3996
Size: 3468 528
AclI AA'CG_TT
Cuts at: 0 3408 3996
Size: 3408 588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 996 2642 2944 3996
Size: 907 89 1646 302 1052
Fragments arranged by size:
1646 1052 907 302 89
AflII C'TTAA_G
Cuts at: 0 2770 3996
Size: 2770 1226
AflIII A'CryG_T
Cuts at: 0 2999 3070 3996
Size: 2999 71 926
Fragments arranged by size:
2999 926 71
AleI CACnn'nnGTG
Cuts at: 0 2098 3996
Size: 2098 1898
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 575 2244 3206 3996
Size: 575 1669 962 790
Fragments arranged by size:
1669 962 790 575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 490 522 1784 1816 3996
Size: 490 32 1262 32 2180
Fragments arranged by size:
2180 1262 490 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 265 396 1019 3996
Size: 265 131 623 2977
Fragments arranged by size:
2977 623 265 131
ApaLI G'TGCA_C
Cuts at: 0 1239 3996
Size: 1239 2757
ApoI r'AATT_y
Cuts at: 0 3815 3840 3996
Size: 3815 25 156
Fragments arranged by size:
3815 156 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2935 2968 3996
Size: 2935 33 1028
Fragments arranged by size:
2935 1028 33
BamHI G'GATC_C
Cuts at: 0 2427 2736 3996
Size: 2427 309 1260
Fragments arranged by size:
2427 1260 309
BanI G'GyrC_C
Cuts at: 0 2686 3037 3468 3996
Size: 2686 351 431 528
Fragments arranged by size:
2686 528 431 351
BanII G_rGCy'C
Cuts at: 0 654 2169 3020 3431 3996
Size: 654 1515 851 411 565
Fragments arranged by size:
1515 851 654 565 411
BbsI GAAGACnn'nnnn_
Cuts at: 0 923 1200 1530 1538 2386 2542 3916 3996
Size: 923 277 330 8 848 156 1374 80
Fragments arranged by size:
1374 923 848 330 277 156 80 8
BbvCI CC'TCA_GC
Cuts at: 0 2848 3996
Size: 2848 1148
BclI T'GATC_A
Cuts at: 0 2268 2673 3996
Size: 2268 405 1323
Fragments arranged by size:
2268 1323 405
BfrBI ATG'CAT
Cuts at: 0 552 2231 3996
Size: 552 1679 1765
Fragments arranged by size:
1765 1679 552
BglI GCCn_nnn'nGGC
Cuts at: 0 3015 3448 3996
Size: 3015 433 548
Fragments arranged by size:
3015 548 433
BlpI GC'TnA_GC
Cuts at: 0 3935 3982 3996
Size: 3935 47 14
Fragments arranged by size:
3935 47 14
Bme1580I G_kGCm'C
Cuts at: 0 1243 2105 3042 3219 3996
Size: 1243 862 937 177 777
Fragments arranged by size:
1243 937 862 777 177
BmgBI CAC'GTC
Cuts at: 0 1925 3996
Size: 1925 2071
BmrI ACTGGGnnnn_n'
Cuts at: 0 727 1402 3966 3996
Size: 727 675 2564 30
Fragments arranged by size:
2564 727 675 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 287 325 451 3543 3658 3676 3996
Size: 49 238 38 126 3092 115 18 320
Fragments arranged by size:
3092 320 238 126 115 49 38 18
Bpu10I CC'TnA_GC
Cuts at: 0 2029 2848 3996
Size: 2029 819 1148
Fragments arranged by size:
2029 1148 819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 979 3415 3996
Size: 126 853 2436 581
Fragments arranged by size:
2436 853 581 126
BsaHI Gr'CG_yC
Cuts at: 0 272 1535 3996
Size: 272 1263 2461
Fragments arranged by size:
2461 1263 272
BsaWI w'CCGG_w
Cuts at: 0 1133 3996
Size: 1133 2863
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 490 520 2986 3016 3996
Size: 232 30 228 30 2466 30 980
Fragments arranged by size:
2466 980 232 228 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1427 3996
Size: 1427 2569
BseYI C'CCAG_C
Cuts at: 0 3107 3443 3996
Size: 3107 336 553
Fragments arranged by size:
3107 553 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2378 3697 3996
Size: 2378 1319 299
Fragments arranged by size:
2378 1319 299
BsiHKAI G_wGCw'C
Cuts at: 0 101 1243 2984 2999 3242 3431 3996
Size: 101 1142 1741 15 243 189 565
Fragments arranged by size:
1741 1142 565 243 189 101 15
BsmI GAATG_Cn'
Cuts at: 0 888 2233 2884 3996
Size: 888 1345 651 1112
Fragments arranged by size:
1345 1112 888 651
BsmBI CGTCTCn'nnnn_
Cuts at: 0 263 3996
Size: 263 3733
Bsp1286I G_dGCh'C
Cuts at: 0 101 654 1243 2105 2169 2984 2999 3020
Size: 101 553 589 862 64 815 15 21
Cuts at: 3020 3042 3219 3242 3431 3996
Size: 22 177 23 189 565
Fragments arranged by size:
862 815 589 565 553 189 177 101
64 23 22 21 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1984 3442 3996
Size: 1984 1458 554
Fragments arranged by size:
1984 1458 554
BsrBI CCG'CTC
Cuts at: 0 3603 3996
Size: 3603 393
BsrDI GCAATG_nn'
Cuts at: 0 2966 3996
Size: 2966 1030
BsrFI r'CCGG_y
Cuts at: 0 1070 2023 3996
Size: 1070 953 1973
Fragments arranged by size:
1973 1070 953
BssSI C'ACGA_G
Cuts at: 0 2575 3043 3996
Size: 2575 468 953
Fragments arranged by size:
2575 953 468
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2988 3996
Size: 2988 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3204 3818 3996
Size: 3204 614 178
Fragments arranged by size:
3204 614 178
BstYI r'GATC_y
Cuts at: 0 440 2427 2736 3996
Size: 440 1987 309 1260
Fragments arranged by size:
1987 1260 440 309
BstZ17I GTA'TAC
Cuts at: 0 3288 3708 3996
Size: 3288 420 288
Fragments arranged by size:
3288 420 288
Bsu36I CC'TnA_GG
Cuts at: 0 744 1993 2279 3996
Size: 744 1249 286 1717
Fragments arranged by size:
1717 1249 744 286
BtgI C'CryG_G
Cuts at: 0 510 1774 2049 3197 3474 3996
Size: 510 1264 275 1148 277 522
Fragments arranged by size:
1264 1148 522 510 277 275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2474 3996
Size: 2474 1522
BtsI GCAGTG_nn'
Cuts at: 0 2415 3516 3531 3996
Size: 2415 1101 15 465
Fragments arranged by size:
2415 1101 465 15
DraI TTT'AAA
Cuts at: 0 1419 2010 2214 3719 3996
Size: 1419 591 204 1505 277
Fragments arranged by size:
1505 1419 591 277 204
DraIII CAC_nnn'GTG
Cuts at: 0 1269 3667 3996
Size: 1269 2398 329
Fragments arranged by size:
2398 1269 329
EaeI y'GGCC_r
Cuts at: 0 204 254 1725 1835 2841 3194 3996
Size: 204 50 1471 110 1006 353 802
Fragments arranged by size:
1471 1006 802 353 204 110 50
EarI CTCTTCn'nnn_
Cuts at: 0 1021 1613 1844 2618 3203 3499 3996
Size: 1021 592 231 774 585 296 497
Fragments arranged by size:
1021 774 592 585 497 296 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1217 3996
Size: 1217 2779
EcoICRI GAG'CTC
Cuts at: 0 3429 3996
Size: 3429 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 287 325 451 907 996 2642 2944
Size: 49 238 38 126 456 89 1646 302
Cuts at: 2944 3543 3658 3676 3996
Size: 599 115 18 320
Fragments arranged by size:
1646 599 456 320 302 238 126 115
89 49 38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 710 2989 3655 3996
Size: 710 2279 666 341
Fragments arranged by size:
2279 710 666 341
EcoO109I rG'GnC_Cy
Cuts at: 0 1053 3642 3862 3996
Size: 1053 2589 220 134
Fragments arranged by size:
2589 1053 220 134
EcoRI G'AATT_C
Cuts at: 0 3840 3996
Size: 3840 156
EcoRV GAT'ATC
Cuts at: 0 2961 3566 3996
Size: 2961 605 430
Fragments arranged by size:
2961 605 430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1444 2767 3744 3996
Size: 1444 1323 977 252
Fragments arranged by size:
1444 1323 977 252
FseI GG_CCGG'CC
Cuts at: 0 2027 3996
Size: 2027 1969
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 226 258 336 368 1026 1058
Size: 124 32 70 32 78 32 658 32
Cuts at: 1058 1445 1477 1897 1929 2022 2054 2235 2253
Size: 387 32 420 32 93 32 181 18
Cuts at: 2253 2267 2285 2475 2507 3481 3513 3996
Size: 14 18 190 32 974 32 483
Fragments arranged by size:
974 658 483 420 387 190 181 124
93 78 70 32 32 32 32 32
32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 0 388 695 1958 3996
Size: 388 307 1263 2038
Fragments arranged by size:
2038 1263 388 307
HindIII A'AGCT_T
Cuts at: 0 2116 3272 3996
Size: 2116 1156 724
Fragments arranged by size:
2116 1156 724
KpnI G_GTAC'C
Cuts at: 0 3472 3996
Size: 3472 524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 462 954 2177 3996
Size: 328 134 492 1223 1819
Fragments arranged by size:
1819 1223 492 328 134
MscI TGG'CCA
Cuts at: 0 256 1727 1837 2843 3996
Size: 256 1471 110 1006 1153
Fragments arranged by size:
1471 1153 1006 256 110
MslI CAynn'nnrTG
Cuts at: 0 449 2098 2226 3002 3188 3996
Size: 449 1649 128 776 186 808
Fragments arranged by size:
1649 808 776 449 186 128
MspA1I CmG'CkG
Cuts at: 0 303 1500 1654 1716 2796 3013 3132 3636
Size: 303 1197 154 62 1080 217 119 504
Cuts at: 3636 3996
Size: 360
Fragments arranged by size:
1197 1080 504 360 303 217 154 119
62
NaeI GCC'GGC
Cuts at: 0 2025 3996
Size: 2025 1971
NcoI C'CATG_G
Cuts at: 0 510 1774 2049 3197 3996
Size: 510 1264 275 1148 799
Fragments arranged by size:
1264 1148 799 510 275
NgoMIV G'CCGG_C
Cuts at: 0 2023 3996
Size: 2023 1973
NsiI A_TGCA'T
Cuts at: 0 554 2233 3996
Size: 554 1679 1763
Fragments arranged by size:
1763 1679 554
NspI r_CATG'y
Cuts at: 0 1081 3003 3074 3996
Size: 1081 1922 71 922
Fragments arranged by size:
1922 1081 922 71
PasI CC'CwG_GG
Cuts at: 0 569 3996
Size: 569 3427
PciI A'CATG_T
Cuts at: 0 2999 3070 3996
Size: 2999 71 926
Fragments arranged by size:
2999 926 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 196 366 3135 3996
Size: 196 170 2769 861
Fragments arranged by size:
2769 861 196 170
PfoI T'CCnGG_A
Cuts at: 0 428 3559 3653 3996
Size: 428 3131 94 343
Fragments arranged by size:
3131 428 343 94
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 3996
Size: 490 32 3474
Fragments arranged by size:
3474 490 32
PpuMI rG'GwC_Cy
Cuts at: 0 1053 3642 3862 3996
Size: 1053 2589 220 134
Fragments arranged by size:
2589 1053 220 134
PshAI GACnn'nnGTC
Cuts at: 0 1880 3996
Size: 1880 2116
PsiI TTA'TAA
Cuts at: 0 1352 3996
Size: 1352 2644
PstI C_TGCA'G
Cuts at: 0 286 1599 1653 3791 3996
Size: 286 1313 54 2138 205
Fragments arranged by size:
2138 1313 286 205 54
PvuII CAG'CTG
Cuts at: 0 303 1716 3132 3996
Size: 303 1413 1416 864
Fragments arranged by size:
1416 1413 864 303
SacI G_AGCT'C
Cuts at: 0 3431 3996
Size: 3431 565
SanDI GG'GwC_CC
Cuts at: 0 1053 3996
Size: 1053 2943
SapI GCTCTTCn'nnn_
Cuts at: 0 1021 1613 3996
Size: 1021 592 2383
Fragments arranged by size:
2383 1021 592
ScaI AGT'ACT
Cuts at: 0 2869 3996
Size: 2869 1127
SfcI C'TryA_G
Cuts at: 0 282 1382 1595 1649 2804 3787 3996
Size: 282 1100 213 54 1155 983 209
Fragments arranged by size:
1155 1100 983 282 213 209 54
SmlI C'TyrA_G
Cuts at: 0 141 994 2770 3430 3996
Size: 141 853 1776 660 566
Fragments arranged by size:
1776 853 660 566 141
SspI AAT'ATT
Cuts at: 0 2638 3996
Size: 2638 1358
StuI AGG'CCT
Cuts at: 0 1511 2629 3772 3996
Size: 1511 1118 1143 224
Fragments arranged by size:
1511 1143 1118 224
StyI C'CwwG_G
Cuts at: 0 510 1774 1863 2049 2134 3197 3996
Size: 510 1264 89 186 85 1063 799
Fragments arranged by size:
1264 1063 799 510 186 89 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1393 1837 1843 3996
Size: 1393 444 6 2153
Fragments arranged by size:
2153 1393 444 6
TatI w'GTAC_w
Cuts at: 0 2559 2571 2867 3996
Size: 2559 12 296 1129
Fragments arranged by size:
2559 1129 296 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 827 859 1178 1210 3996
Size: 232 32 563 32 319 32 2786
Fragments arranged by size:
2786 563 319 232 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 136 1698 2452 3996
Size: 136 1562 754 1544
Fragments arranged by size:
1562 1544 754 136
XbaI T'CTAG_A
Cuts at: 0 1323 3996
Size: 1323 2673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 593 705 731 3129 3996
Size: 593 112 26 2398 867
Fragments arranged by size:
2398 867 593 112 26
XmnI GAAnn'nnTTC
Cuts at: 0 182 920 2153 2750 3996
Size: 182 738 1233 597 1246
Fragments arranged by size:
1246 1233 738 597 182
ZraI GAC'GTC
Cuts at: 0 273 1536 3996
Size: 273 1263 2460
Fragments arranged by size:
2460 1263 273
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI BaeI BamHI BanI
BanII BbsI BbvCI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI Hin4I HincII HindIII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PsiI PstI PvuII
SacI SanDI SapI ScaI SfcI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI ApaI AscI AseI AsiSI
AvaI AvrII BbeI BcgI BciVI BglII BmtI BplI
BsaI BsaAI BsaBI BsiEI BsiWI BspEI BspHI BsrGI
BssHII BstBI BstEII ClaI CspCI DrdI EagI FspI
FspAI HaeII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI PacI PmeI PmlI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI XhoI XmaI