Restriction map for
pF1KSDB0058
(Linear) (Six-base) MAPSORT of: KIBB0058.seq Check: 8144 from: 1 to: 3303
KIBB0058.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 1589 2399 3101 3303
Size: 1589 810 702 202
Fragments arranged by size:
1589 810 702 202
Acc65I G'GTAC_C
Cuts at: 0 2072 3303
Size: 2072 1231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 542 811 1123 1461 1814 3256 3303
Size: 274 268 269 312 338 353 1442 47
Fragments arranged by size:
1442 353 338 312 274 269 268 47
AflIII A'CryG_T
Cuts at: 0 2239 3303
Size: 2239 1064
AleI CACnn'nnGTG
Cuts at: 0 1045 3303
Size: 1045 2258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3011 3199 3303
Size: 3011 188 104
Fragments arranged by size:
3011 188 104
AlwNI CAG_nnn'CTG
Cuts at: 0 453 1579 2531 3246 3303
Size: 453 1126 952 715 57
Fragments arranged by size:
1126 952 715 453 57
ApaI G_GGCC'C
Cuts at: 0 123 1099 3303
Size: 123 976 2204
Fragments arranged by size:
2204 976 123
ApoI r'AATT_y
Cuts at: 0 536 3303
Size: 536 2767
AvaI C'yCGr_G
Cuts at: 0 95 377 598 983 1517 1559 2076 2775
Size: 95 282 221 385 534 42 517 699
Cuts at: 2775 3131 3303
Size: 356 172
Fragments arranged by size:
699 534 517 385 356 282 221 172
95 42
BamHI G'GATC_C
Cuts at: 0 1196 3303
Size: 1196 2107
BanI G'GyrC_C
Cuts at: 0 847 925 1392 2072 2445 2809 3303
Size: 847 78 467 680 373 364 494
Fragments arranged by size:
847 680 494 467 373 364 78
BanII G_rGCy'C
Cuts at: 0 123 326 1099 1571 2009 2083 2555 2721
Size: 123 203 773 472 438 74 472 166
Cuts at: 2721 3168 3303
Size: 447 135
Fragments arranged by size:
773 472 472 447 438 203 166 135
123 74
BbeI G_GCGC'C
Cuts at: 0 851 929 1396 3303
Size: 851 78 467 1907
Fragments arranged by size:
1907 851 467 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 1192 1647 2577 3303
Size: 530 662 455 930 726
Fragments arranged by size:
930 726 662 530 455
BbvCI CC'TCA_GC
Cuts at: 0 66 347 3303
Size: 66 281 2956
Fragments arranged by size:
2956 281 66
BclI T'GATC_A
Cuts at: 0 185 1826 2694 2906 3222 3303
Size: 185 1641 868 212 316 81
Fragments arranged by size:
1641 868 316 212 185 81
BglI GCCn_nnn'nGGC
Cuts at: 0 2299 3303
Size: 2299 1004
BglII A'GATC_T
Cuts at: 0 2406 3303
Size: 2406 897
BlpI GC'TnA_GC
Cuts at: 0 151 3303
Size: 151 3152
Bme1580I G_kGCm'C
Cuts at: 0 123 1099 1634 2164 2360 2465 3303
Size: 123 976 535 530 196 105 838
Fragments arranged by size:
976 838 535 530 196 123 105
BmgBI CAC'GTC
Cuts at: 0 423 2240 3303
Size: 423 1817 1063
Fragments arranged by size:
1817 1063 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 1202 1610 1635 3303
Size: 126 1076 408 25 1668
Fragments arranged by size:
1668 1076 408 126 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2105 2303 2735 2748 3303
Size: 2105 198 432 13 555
Fragments arranged by size:
2105 555 432 198 13
Bpu10I CC'TnA_GC
Cuts at: 0 66 347 3303
Size: 66 281 2956
Fragments arranged by size:
2956 281 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 3303
Size: 289 3014
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 1744 2766 3085 3303
Size: 214 1530 1022 319 218
Fragments arranged by size:
1530 1022 319 218 214
BsaBI GATnn'nnATC
Cuts at: 0 1012 1201 1645 3303
Size: 1012 189 444 1658
Fragments arranged by size:
1658 1012 444 189
BsaHI Gr'CG_yC
Cuts at: 0 437 665 848 926 1339 1393 2039 3303
Size: 437 228 183 78 413 54 646 1264
Fragments arranged by size:
1264 646 437 413 228 183 78 54
BsaWI w'CCGG_w
Cuts at: 0 1772 1987 3285 3303
Size: 1772 215 1298 18
Fragments arranged by size:
1772 1298 215 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 623 653 2065 2095 2339 2369 2414 2444
Size: 623 30 1412 30 244 30 45 30
Cuts at: 2444 2746 2776 3303
Size: 302 30 527
Fragments arranged by size:
1412 623 527 302 244 45 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 1303 2247 2614 2993 3303
Size: 364 939 944 367 379 310
Fragments arranged by size:
944 939 379 367 364 310
BseYI C'CCAG_C
Cuts at: 0 264 931 1348 2746 3186 3303
Size: 264 667 417 1398 440 117
Fragments arranged by size:
1398 667 440 417 264 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2980 3303
Size: 2980 323
BsiHKAI G_wGCw'C
Cuts at: 0 1112 1333 1468 1706 2009 2555 3303
Size: 1112 221 135 238 303 546 748
Fragments arranged by size:
1112 748 546 303 238 221 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1556 2715 3303
Size: 1556 1159 588
Fragments arranged by size:
1556 1159 588
Bsp1286I G_dGCh'C
Cuts at: 0 123 326 1099 1112 1333 1468 1571 1634
Size: 123 203 773 13 221 135 103 63
Cuts at: 1634 1706 2009 2083 2164 2360 2465 2555 2721
Size: 72 303 74 81 196 105 90 166
Cuts at: 2721 3168 3303
Size: 447 135
Fragments arranged by size:
773 447 303 221 203 196 166 135
135 123 105 103 90 81 74 72
63 13
BspEI T'CCGG_A
Cuts at: 0 1772 1987 3285 3303
Size: 1772 215 1298 18
Fragments arranged by size:
1772 1298 215 18
BspHI T'CATG_A
Cuts at: 0 977 2519 3303
Size: 977 1542 784
Fragments arranged by size:
1542 977 784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1875 2442 2517 3303
Size: 1875 567 75 786
Fragments arranged by size:
1875 786 567 75
BsrBI CCG'CTC
Cuts at: 0 1727 3303
Size: 1727 1576
BsrDI GCAATG_nn'
Cuts at: 0 755 3303
Size: 755 2548
BsrFI r'CCGG_y
Cuts at: 0 1262 3303
Size: 1262 2041
BsrGI T'GTAC_A
Cuts at: 0 860 3303
Size: 860 2443
BssSI C'ACGA_G
Cuts at: 0 406 1815 2410 3303
Size: 406 1409 595 893
Fragments arranged by size:
1409 893 595 406
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1533 3303
Size: 1533 1770
BstEII G'GTnAC_C
Cuts at: 0 2920 3303
Size: 2920 383
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1213 2091 3303
Size: 1213 878 1212
Fragments arranged by size:
1213 1212 878
BstYI r'GATC_y
Cuts at: 0 821 1007 1196 1447 1640 1769 2406 2963
Size: 821 186 189 251 193 129 637 557
Cuts at: 2963 3303
Size: 340
Fragments arranged by size:
821 637 557 340 251 193 189 186
129
BstZ17I GTA'TAC
Cuts at: 0 2400 3303
Size: 2400 903
Bsu36I CC'TnA_GG
Cuts at: 0 485 1805 3303
Size: 485 1320 1498
Fragments arranged by size:
1498 1320 485
BtgI C'CryG_G
Cuts at: 0 259 830 1583 1745 2030 2465 2867 3259
Size: 259 571 753 162 285 435 402 392
Cuts at: 3259 3303
Size: 44
Fragments arranged by size:
753 571 435 402 392 285 259 162
44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1014 1764 2549 3303
Size: 1014 750 785 754
Fragments arranged by size:
1014 785 754 750
BtsI GCAGTG_nn'
Cuts at: 0 668 1298 3303
Size: 668 630 2005
Fragments arranged by size:
2005 668 630
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 177 212 1899 1934 3303
Size: 177 35 1687 35 1369
Fragments arranged by size:
1687 1369 177 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2928 3303
Size: 2928 375
EaeI y'GGCC_r
Cuts at: 0 5 542 653 836 1235 2054 2274 2414
Size: 5 537 111 183 399 819 220 140
Cuts at: 2414 3303
Size: 889
Fragments arranged by size:
889 819 537 399 220 183 140 111
5
EarI CTCTTCn'nnn_
Cuts at: 0 568 1128 1480 2588 3223 3303
Size: 568 560 352 1108 635 80
Fragments arranged by size:
1108 635 568 560 352 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1852 3303
Size: 1852 1451
EcoICRI GAG'CTC
Cuts at: 0 2007 2553 3303
Size: 2007 546 750
Fragments arranged by size:
2007 750 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 542 811 1123 1461 1814 2105 2303
Size: 274 268 269 312 338 353 291 198
Cuts at: 2303 2735 2748 3256 3303
Size: 432 13 508 47
Fragments arranged by size:
508 432 353 338 312 291 274 269
268 198 47 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2481 3303
Size: 2481 822
EcoO109I rG'GnC_Cy
Cuts at: 0 1095 1096 1809 2613 3122 3303
Size: 1095 1 713 804 509 181
Fragments arranged by size:
1095 804 713 509 181 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 58 816 3303
Size: 58 758 2487
Fragments arranged by size:
2487 758 58
HaeII r_GCGC'y
Cuts at: 0 851 929 1396 3303
Size: 851 78 467 1907
Fragments arranged by size:
1907 851 467 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 623 655 1013 1045 1555 1587
Size: 177 32 414 32 358 32 510 32
Cuts at: 1587 1841 1873 2372 2404 2537 2569 2679 2711
Size: 254 32 499 32 133 32 110 32
Cuts at: 2711 2746 2778 2948 2980 3303
Size: 35 32 170 32 323
Fragments arranged by size:
510 499 414 358 323 254 177 170
133 110 35 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 222 894 3303
Size: 222 672 2409
Fragments arranged by size:
2409 672 222
KasI G'GCGC_C
Cuts at: 0 847 925 1392 3303
Size: 847 78 467 1911
Fragments arranged by size:
1911 847 467 78
KpnI G_GTAC'C
Cuts at: 0 2076 3303
Size: 2076 1227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1857 2024 3303
Size: 1857 167 1279
Fragments arranged by size:
1857 1279 167
MscI TGG'CCA
Cuts at: 0 544 2056 2276 2416 3303
Size: 544 1512 220 140 887
Fragments arranged by size:
1512 887 544 220 140
MslI CAynn'nnrTG
Cuts at: 0 998 1045 2089 2671 2956 3181 3298 3303
Size: 998 47 1044 582 285 225 117 5
Fragments arranged by size:
1044 998 582 285 225 117 47 5
MspA1I CmG'CkG
Cuts at: 0 183 1291 1325 2692 3303
Size: 183 1108 34 1367 611
Fragments arranged by size:
1367 1108 611 183 34
NarI GG'CG_CC
Cuts at: 0 848 926 1393 3303
Size: 848 78 467 1910
Fragments arranged by size:
1910 848 467 78
NcoI C'CATG_G
Cuts at: 0 830 1583 1745 2465 2867 3303
Size: 830 753 162 720 402 436
Fragments arranged by size:
830 753 720 436 402 162
NdeI CA'TA_TG
Cuts at: 0 3276 3303
Size: 3276 27
NspI r_CATG'y
Cuts at: 0 1512 1536 1998 2571 2955 3108 3303
Size: 1512 24 462 573 384 153 195
Fragments arranged by size:
1512 573 462 384 195 153 24
PasI CC'CwG_GG
Cuts at: 0 1254 3303
Size: 1254 2049
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 1502 3303
Size: 162 1340 1801
Fragments arranged by size:
1801 1340 162
PfoI T'CCnGG_A
Cuts at: 0 2121 3303
Size: 2121 1182
PpuMI rG'GwC_Cy
Cuts at: 0 2613 3122 3303
Size: 2613 509 181
Fragments arranged by size:
2613 509 181
PspOMI G'GGCC_C
Cuts at: 0 119 1095 3303
Size: 119 976 2208
Fragments arranged by size:
2208 976 119
PstI C_TGCA'G
Cuts at: 0 134 2316 3303
Size: 134 2182 987
Fragments arranged by size:
2182 987 134
PvuII CAG'CTG
Cuts at: 0 183 2692 3303
Size: 183 2509 611
Fragments arranged by size:
2509 611 183
SacI G_AGCT'C
Cuts at: 0 2009 2555 3303
Size: 2009 546 748
Fragments arranged by size:
2009 748 546
ScaI AGT'ACT
Cuts at: 0 919 2653 3303
Size: 919 1734 650
Fragments arranged by size:
1734 919 650
SexAI A'CCwGG_T
Cuts at: 0 2756 3303
Size: 2756 547
SfcI C'TryA_G
Cuts at: 0 130 2312 3303
Size: 130 2182 991
Fragments arranged by size:
2182 991 130
SfoI GGC'GCC
Cuts at: 0 849 927 1394 3303
Size: 849 78 467 1909
Fragments arranged by size:
1909 849 467 78
SmaI CCC'GGG
Cuts at: 0 1519 2078 3303
Size: 1519 559 1225
Fragments arranged by size:
1519 1225 559
SmlI C'TyrA_G
Cuts at: 0 304 598 3303
Size: 304 294 2705
Fragments arranged by size:
2705 304 294
SphI G_CATG'C
Cuts at: 0 1512 1536 2571 3303
Size: 1512 24 1035 732
Fragments arranged by size:
1512 1035 732 24
StyI C'CwwG_G
Cuts at: 0 8 440 656 830 1490 1583 1745 2465
Size: 8 432 216 174 660 93 162 720
Cuts at: 2465 2813 2867 3303
Size: 348 54 436
Fragments arranged by size:
720 660 436 432 348 216 174 162
93 54 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 1010 1299 1883 3303
Size: 415 595 289 584 1420
Fragments arranged by size:
1420 595 584 415 289
TatI w'GTAC_w
Cuts at: 0 584 860 917 2651 3303
Size: 584 276 57 1734 652
Fragments arranged by size:
1734 652 584 276 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2339 2371 3303
Size: 2339 32 932
Fragments arranged by size:
2339 932 32
Tth111I GACn'n_nGTC
Cuts at: 0 391 1549 2758 3303
Size: 391 1158 1209 545
Fragments arranged by size:
1209 1158 545 391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1245 3303
Size: 1245 2058
XhoI C'TCGA_G
Cuts at: 0 598 3303
Size: 598 2705
XmaI C'CCGG_G
Cuts at: 0 1517 2076 3303
Size: 1517 559 1227
Fragments arranged by size:
1517 1227 559
XmnI GAAnn'nnTTC
Cuts at: 0 82 3303
Size: 82 3221
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NarI NcoI NdeI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI ScaI SexAI SfcI
SfoI SmaI SmlI SphI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BfrBI BmtI BplI BsaAI BsiEI BsiWI BsmI BssHII
BstBI ClaI DraI DrdI EagI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI MfeI MluI NaeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI XbaI ZraI