Restriction map for
pF1KSDB0060
(Linear) (Six-base) MAPSORT of: KIBB0060.seq Check: 3478 from: 1 to: 2721
KIBB0060.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 1390 2721
Size: 1390 1331
Acc65I G'GTAC_C
Cuts at: 0 1691 2692 2721
Size: 1691 1001 29
Fragments arranged by size:
1691 1001 29
AclI AA'CG_TT
Cuts at: 0 29 2721
Size: 29 2692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1896 2338 2616 2721
Size: 1153 743 442 278 105
Fragments arranged by size:
1153 743 442 278 105
AflIII A'CryG_T
Cuts at: 0 2051 2721
Size: 2051 670
AhdI GACnn_n'nnGTC
Cuts at: 0 18 2721
Size: 18 2703
AlwNI CAG_nnn'CTG
Cuts at: 0 45 211 2721
Size: 45 166 2510
Fragments arranged by size:
2510 166 45
ApoI r'AATT_y
Cuts at: 0 151 1062 1333 1470 1796 2503 2721
Size: 151 911 271 137 326 707 218
Fragments arranged by size:
911 707 326 271 218 151 137
AvaI C'yCGr_G
Cuts at: 0 1958 2721
Size: 1958 763
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2550 2583 2721
Size: 2550 33 138
Fragments arranged by size:
2550 138 33
BamHI G'GATC_C
Cuts at: 0 56 2721
Size: 56 2665
BanI G'GyrC_C
Cuts at: 0 1094 1691 2692 2721
Size: 1094 597 1001 29
Fragments arranged by size:
1094 1001 597 29
BanII G_rGCy'C
Cuts at: 0 460 2672 2721
Size: 460 2212 49
Fragments arranged by size:
2212 460 49
BbeI G_GCGC'C
Cuts at: 0 1098 2721
Size: 1098 1623
BbsI GAAGACnn'nnnn_
Cuts at: 0 1141 1902 2608 2721
Size: 1141 761 706 113
Fragments arranged by size:
1141 761 706 113
BbvCI CC'TCA_GC
Cuts at: 0 1191 1255 2721
Size: 1191 64 1466
Fragments arranged by size:
1466 1191 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 15 49 108 142 2721
Size: 15 34 59 34 2579
Fragments arranged by size:
2579 59 34 34 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 705 2721
Size: 705 2016
BclI T'GATC_A
Cuts at: 0 1494 2337 2721
Size: 1494 843 384
Fragments arranged by size:
1494 843 384
BfrBI ATG'CAT
Cuts at: 0 1585 2721
Size: 1585 1136
BlpI GC'TnA_GC
Cuts at: 0 292 2721
Size: 292 2429
Bme1580I G_kGCm'C
Cuts at: 0 444 507 2310 2721
Size: 444 63 1803 411
Fragments arranged by size:
1803 444 411 63
BmgBI CAC'GTC
Cuts at: 0 1866 2721
Size: 1866 855
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 662 2721
Size: 438 224 2059
Fragments arranged by size:
2059 438 224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 2721
Size: 1206 1515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1871 1897 2022 2286 2721
Size: 548 1323 26 125 264 435
Fragments arranged by size:
1323 548 435 264 125 26
Bpu10I CC'TnA_GC
Cuts at: 0 356 421 1191 1255 2721
Size: 356 65 770 64 1466
Fragments arranged by size:
1466 770 356 65 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1336 2348 2721
Size: 1093 243 1012 373
Fragments arranged by size:
1093 1012 373 243
BsaI GGTCTCn'nnnn_
Cuts at: 0 1214 1241 1612 1887 2721
Size: 1214 27 371 275 834
Fragments arranged by size:
1214 834 371 275 27
BsaBI GATnn'nnATC
Cuts at: 0 2231 2721
Size: 2231 490
BsaHI Gr'CG_yC
Cuts at: 0 1095 2721
Size: 1095 1626
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 521 551 2284 2314 2721
Size: 521 30 1733 30 407
Fragments arranged by size:
1733 521 407 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 945 2494 2721
Size: 529 416 1549 227
Fragments arranged by size:
1549 529 416 227
BseYI C'CCAG_C
Cuts at: 0 86 2721
Size: 86 2635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1264 2086 2231 2593 2721
Size: 1264 822 145 362 128
Fragments arranged by size:
1264 822 362 145 128
BsiHKAI G_wGCw'C
Cuts at: 0 460 2533 2721
Size: 460 2073 188
Fragments arranged by size:
2073 460 188
BsmI GAATG_Cn'
Cuts at: 0 2364 2721
Size: 2364 357
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2397 2721
Size: 2397 324
Bsp1286I G_dGCh'C
Cuts at: 0 444 460 507 2310 2533 2672 2721
Size: 444 16 47 1803 223 139 49
Fragments arranged by size:
1803 444 223 139 49 47 16
BspHI T'CATG_A
Cuts at: 0 1491 2721
Size: 1491 1230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1703 2721
Size: 1703 1018
BsrBI CCG'CTC
Cuts at: 0 944 2721
Size: 944 1777
BsrDI GCAATG_nn'
Cuts at: 0 1688 2064 2240 2721
Size: 1688 376 176 481
Fragments arranged by size:
1688 481 376 176
BssSI C'ACGA_G
Cuts at: 0 1261 2251 2721
Size: 1261 990 470
Fragments arranged by size:
1261 990 470
BstAPI GCAn_nnn'nTGC
Cuts at: 0 211 2721
Size: 211 2510
BstYI r'GATC_y
Cuts at: 0 56 1350 1847 2721
Size: 56 1294 497 874
Fragments arranged by size:
1294 874 497 56
BstZ17I GTA'TAC
Cuts at: 0 1391 2721
Size: 1391 1330
BtsI GCAGTG_nn'
Cuts at: 0 483 1158 2721
Size: 483 675 1563
Fragments arranged by size:
1563 675 483
ClaI AT'CG_AT
Cuts at: 0 1008 1405 2721
Size: 1008 397 1316
Fragments arranged by size:
1316 1008 397
DraI TTT'AAA
Cuts at: 0 2315 2508 2721
Size: 2315 193 213
Fragments arranged by size:
2315 213 193
DraIII CAC_nnn'GTG
Cuts at: 0 2256 2721
Size: 2256 465
DrdI GACnn_nn'nnGTC
Cuts at: 0 871 2721
Size: 871 1850
EarI CTCTTCn'nnn_
Cuts at: 0 192 2414 2471 2721
Size: 192 2222 57 250
Fragments arranged by size:
2222 250 192 57
EcoICRI GAG'CTC
Cuts at: 0 458 2721
Size: 458 2263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1153 1871 1896 1897 2022 2286 2338
Size: 548 605 718 25 1 125 264 52
Cuts at: 2338 2616 2721
Size: 278 105
Fragments arranged by size:
718 605 548 278 264 125 105 52
25 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 2721
Size: 301 2420
EcoO109I rG'GnC_Cy
Cuts at: 0 306 1446 2721
Size: 306 1140 1275
Fragments arranged by size:
1275 1140 306
EcoRI G'AATT_C
Cuts at: 0 1796 2721
Size: 1796 925
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 872 1677 2721
Size: 872 805 1044
Fragments arranged by size:
1044 872 805
HaeII r_GCGC'y
Cuts at: 0 1098 2721
Size: 1098 1623
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 364 396 1101 1133 1416 1448
Size: 4 32 328 32 705 32 283 32
Cuts at: 1448 1761 1793 1892 1924 2284 2316 2721
Size: 313 32 99 32 360 32 405
Fragments arranged by size:
705 405 360 328 313 283 99 32
32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 1932 2721
Size: 1932 789
KasI G'GCGC_C
Cuts at: 0 1094 2721
Size: 1094 1627
KpnI G_GTAC'C
Cuts at: 0 1695 2696 2721
Size: 1695 1001 25
Fragments arranged by size:
1695 1001 25
MfeI C'AATT_G
Cuts at: 0 1940 2721
Size: 1940 781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1616 2721
Size: 1378 238 1105
Fragments arranged by size:
1378 1105 238
MslI CAynn'nnrTG
Cuts at: 0 799 2721
Size: 799 1922
MspA1I CmG'CkG
Cuts at: 0 1195 1295 1810 2282 2721
Size: 1195 100 515 472 439
Fragments arranged by size:
1195 515 472 439 100
NarI GG'CG_CC
Cuts at: 0 1095 2721
Size: 1095 1626
NsiI A_TGCA'T
Cuts at: 0 1587 2721
Size: 1587 1134
NspI r_CATG'y
Cuts at: 0 1233 1677 2055 2082 2721
Size: 1233 444 378 27 639
Fragments arranged by size:
1233 639 444 378 27
PciI A'CATG_T
Cuts at: 0 2051 2721
Size: 2051 670
PflMI CCAn_nnn'nTGG
Cuts at: 0 2229 2721
Size: 2229 492
PfoI T'CCnGG_A
Cuts at: 0 564 697 2721
Size: 564 133 2024
Fragments arranged by size:
2024 564 133
PpuMI rG'GwC_Cy
Cuts at: 0 306 1446 2721
Size: 306 1140 1275
Fragments arranged by size:
1275 1140 306
PshAI GACnn'nnGTC
Cuts at: 0 2612 2721
Size: 2612 109
PsiI TTA'TAA
Cuts at: 0 1720 2721
Size: 1720 1001
PstI C_TGCA'G
Cuts at: 0 41 55 1539 1560 2721
Size: 41 14 1484 21 1161
Fragments arranged by size:
1484 1161 41 21 14
PvuII CAG'CTG
Cuts at: 0 1195 2721
Size: 1195 1526
SacI G_AGCT'C
Cuts at: 0 460 2721
Size: 460 2261
SfcI C'TryA_G
Cuts at: 0 37 51 1535 1556 2721
Size: 37 14 1484 21 1165
Fragments arranged by size:
1484 1165 37 21 14
SfoI GGC'GCC
Cuts at: 0 1096 2721
Size: 1096 1625
SmlI C'TyrA_G
Cuts at: 0 1072 1315 1958 2363 2721
Size: 1072 243 643 405 358
Fragments arranged by size:
1072 643 405 358 243
SspI AAT'ATT
Cuts at: 0 2546 2721
Size: 2546 175
StuI AGG'CCT
Cuts at: 0 361 405 2721
Size: 361 44 2316
Fragments arranged by size:
2316 361 44
StyI C'CwwG_G
Cuts at: 0 1164 2721
Size: 1164 1557
TatI w'GTAC_w
Cuts at: 0 1655 2721
Size: 1655 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 521 553 1164 1196 2721
Size: 521 32 611 32 1525
Fragments arranged by size:
1525 611 521 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 693 1530 2721
Size: 693 837 1191
Fragments arranged by size:
1191 837 693
XbaI T'CTAG_A
Cuts at: 0 1800 2721
Size: 1800 921
XhoI C'TCGA_G
Cuts at: 0 1958 2721
Size: 1958 763
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BssSI BstAPI BstYI BstZ17I BtsI
ClaI DraI DraIII DrdI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI KpnI
MfeI MmeI MslI MspA1I NarI NsiI NspI PciI
PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacI
SfcI SfoI SmlI SspI StuI StyI TatI TstI
Tth111I XbaI XhoI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BglI BglII
BmtI BsaAI BsaWI BsiEI BsiWI BspEI BsrFI BsrGI
BssHII BstBI BstEII BstXI Bsu36I BtgI BtgZI CspCI
EaeI EagI EciI EcoRV FseI FspI FspAI HindIII
HpaI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII XcmI XmaI XmnI
ZraI