Restriction map for
pF1KB0065
(Linear) (Six-base) MAPSORT of: KIBB0065.seq Check: 9277 from: 1 to: 6123
KIBB0065.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2814 5730 6123
Size: 2814 2916 393
Fragments arranged by size:
2916 2814 393
AatII G_ACGT'C
Cuts at: 0 1427 5827 6003 6123
Size: 1427 4400 176 120
Fragments arranged by size:
4400 1427 176 120
AccI GT'mk_AC
Cuts at: 0 3181 6123
Size: 3181 2942
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 127 136 182 1190 1291 1556 1764
Size: 117 10 9 46 1008 101 265 208
Cuts at: 1764 2332 2445 3774 3890 4666 4697 5892 6011
Size: 568 113 1329 116 776 31 1195 119
Cuts at: 6011 6123
Size: 112
Fragments arranged by size:
1329 1195 1008 776 568 265 208 119
117 116 113 112 101 46 31 10
9
AfeI AGC'GCT
Cuts at: 0 46 6123
Size: 46 6077
AflIII A'CryG_T
Cuts at: 0 3564 6123
Size: 3564 2559
AhdI GACnn_n'nnGTC
Cuts at: 0 4570 6123
Size: 4570 1553
AleI CACnn'nnGTG
Cuts at: 0 2426 2848 6123
Size: 2426 422 3275
Fragments arranged by size:
3275 2426 422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5762 5807 6123
Size: 5762 45 316
Fragments arranged by size:
5762 316 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 482 514 6123
Size: 482 32 5609
Fragments arranged by size:
5609 482 32
AlwNI CAG_nnn'CTG
Cuts at: 0 144 171 322 2332 4587 4720 4761 5989
Size: 144 27 151 2010 2255 133 41 1228
Cuts at: 5989 6123
Size: 134
Fragments arranged by size:
2255 2010 1228 151 144 134 133 41
27
ApaI G_GGCC'C
Cuts at: 0 30 5518 6123
Size: 30 5488 605
Fragments arranged by size:
5488 605 30
ApoI r'AATT_y
Cuts at: 0 361 560 2661 6123
Size: 361 199 2101 3462
Fragments arranged by size:
3462 2101 361 199
AseI AT'TA_AT
Cuts at: 0 338 3026 3170 5317 6123
Size: 338 2688 144 2147 806
Fragments arranged by size:
2688 2147 806 338 144
AvrII C'CTAG_G
Cuts at: 0 3051 5205 6123
Size: 3051 2154 918
Fragments arranged by size:
3051 2154 918
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5228 5261 6123
Size: 5228 33 862
Fragments arranged by size:
5228 862 33
BamHI G'GATC_C
Cuts at: 0 2013 2154 5422 6123
Size: 2013 141 3268 701
Fragments arranged by size:
3268 2013 701 141
BanI G'GyrC_C
Cuts at: 0 1006 2199 3118 3840 4954 6123
Size: 1006 1193 919 722 1114 1169
Fragments arranged by size:
1193 1169 1114 1006 919 722
BanII G_rGCy'C
Cuts at: 0 30 127 419 1409 2935 2959 5518 6123
Size: 30 97 292 990 1526 24 2559 605
Fragments arranged by size:
2559 1526 990 605 292 97 30 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 1427 3231 4116 4654 5013 5298 5339
Size: 95 1332 1804 885 538 359 285 41
Cuts at: 5339 5999 6123
Size: 660 124
Fragments arranged by size:
1804 1332 885 660 538 359 285 124
95 41
BbvCI CC'TCA_GC
Cuts at: 0 677 6123
Size: 677 5446
BciVI GTATCCnnnnn_n'
Cuts at: 0 1559 6123
Size: 1559 4564
BclI T'GATC_A
Cuts at: 0 366 555 588 870 3930 6123
Size: 366 189 33 282 3060 2193
Fragments arranged by size:
3060 2193 366 282 189 33
BfrBI ATG'CAT
Cuts at: 0 5467 5719 6123
Size: 5467 252 404
Fragments arranged by size:
5467 404 252
BglI GCCn_nnn'nGGC
Cuts at: 0 3838 6123
Size: 3838 2285
BglII A'GATC_T
Cuts at: 0 924 1977 5027 6123
Size: 924 1053 3050 1096
Fragments arranged by size:
3050 1096 1053 924
BlpI GC'TnA_GC
Cuts at: 0 2351 3526 6123
Size: 2351 1175 2597
Fragments arranged by size:
2597 2351 1175
Bme1580I G_kGCm'C
Cuts at: 0 30 2488 3893 4263 5518 6123
Size: 30 2458 1405 370 1255 605
Fragments arranged by size:
2458 1405 1255 605 370 30
BmgBI CAC'GTC
Cuts at: 0 5760 6123
Size: 5760 363
BmrI ACTGGGnnnn_n'
Cuts at: 0 3839 4749 5539 5825 6123
Size: 3839 910 790 286 298
Fragments arranged by size:
3839 910 790 298 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 878 2128 2719 3483 3632 4078 4471
Size: 455 423 1250 591 764 149 446 393
Cuts at: 4471 5039 5261 5567 5627 5771 6123
Size: 568 222 306 60 144 352
Fragments arranged by size:
1250 764 591 568 455 446 423 393
352 306 222 149 144 60
Bpu10I CC'TnA_GC
Cuts at: 0 677 2393 4921 5901 6123
Size: 677 1716 2528 980 222
Fragments arranged by size:
2528 1716 980 677 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2311 2549 5073 6123
Size: 2311 238 2524 1050
Fragments arranged by size:
2524 2311 1050 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 890 1268 5300 6123
Size: 890 378 4032 823
Fragments arranged by size:
4032 890 823 378
BsaAI yAC'GTr
Cuts at: 0 1974 6123
Size: 1974 4149
BsaHI Gr'CG_yC
Cuts at: 0 1424 5098 5824 6000 6123
Size: 1424 3674 726 176 123
Fragments arranged by size:
3674 1424 726 176 123
BsaWI w'CCGG_w
Cuts at: 0 744 4791 6123
Size: 744 4047 1332
Fragments arranged by size:
4047 1332 744
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5143 5173 5234 5264 5744 5774 6123
Size: 5143 30 61 30 480 30 349
Fragments arranged by size:
5143 480 349 61 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 494 875 1044 1395 2630 5164 5564
Size: 409 85 381 169 351 1235 2534 400
Cuts at: 5564 5768 5849 6123
Size: 204 81 274
Fragments arranged by size:
2534 1235 409 400 381 351 274 204
169 85 81
BseYI C'CCAG_C
Cuts at: 0 243 486 4093 4914 4950 6123
Size: 243 243 3607 821 36 1173
Fragments arranged by size:
3607 1173 821 243 243 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2912 3748 3903 4745 6123
Size: 2912 836 155 842 1378
Fragments arranged by size:
2912 1378 842 836 155
BsiHKAI G_wGCw'C
Cuts at: 0 419 1367 1409 2322 2959 6123
Size: 419 948 42 913 637 3164
Fragments arranged by size:
3164 948 913 637 419 42
BsmI GAATG_Cn'
Cuts at: 0 2090 4242 5469 6123
Size: 2090 2152 1227 654
Fragments arranged by size:
2152 2090 1227 654
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1781 5750 6123
Size: 1781 3969 373
Fragments arranged by size:
3969 1781 373
Bsp1286I G_dGCh'C
Cuts at: 0 30 127 419 1367 1409 2322 2488 2935
Size: 30 97 292 948 42 913 166 447
Cuts at: 2935 2959 3893 4263 5518 6123
Size: 24 934 370 1255 605
Fragments arranged by size:
1255 948 934 913 605 447 370 292
166 97 42 30 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 357 2814 2951 4864 5730 6123
Size: 357 2457 137 1913 866 393
Fragments arranged by size:
2457 1913 866 393 357 137
BsrDI GCAATG_nn'
Cuts at: 0 670 976 1169 2210 3537 5597 6023 6123
Size: 670 306 193 1041 1327 2060 426 100
Fragments arranged by size:
2060 1327 1041 670 426 306 193 100
BsrGI T'GTAC_A
Cuts at: 0 162 6123
Size: 162 5961
BssSI C'ACGA_G
Cuts at: 0 3209 6123
Size: 3209 2914
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1006 1033 4723 4837 6123
Size: 1006 27 3690 114 1286
Fragments arranged by size:
3690 1286 1006 114 27
BstBI TT'CG_AA
Cuts at: 0 3099 6123
Size: 3099 3024
BstEII G'GTnAC_C
Cuts at: 0 2242 4615 6123
Size: 2242 2373 1508
Fragments arranged by size:
2373 2242 1508
BstXI CCAn_nnnn'nTGG
Cuts at: 0 535 1038 3453 6123
Size: 535 503 2415 2670
Fragments arranged by size:
2670 2415 535 503
BstYI r'GATC_y
Cuts at: 0 790 924 1977 2013 2154 5027 5422 6123
Size: 790 134 1053 36 141 2873 395 701
Fragments arranged by size:
2873 1053 790 701 395 141 134 36
Bsu36I CC'TnA_GG
Cuts at: 0 964 2645 3483 6123
Size: 964 1681 838 2640
Fragments arranged by size:
2640 1681 964 838
BtgI C'CryG_G
Cuts at: 0 4553 6123
Size: 4553 1570
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2041 5526 6123
Size: 2041 3485 597
Fragments arranged by size:
3485 2041 597
BtsI GCAGTG_nn'
Cuts at: 0 525 897 948 1031 1245 3891 4310 6123
Size: 525 372 51 83 214 2646 419 1813
Fragments arranged by size:
2646 1813 525 419 372 214 83 51
DraI TTT'AAA
Cuts at: 0 4434 6123
Size: 4434 1689
DraIII CAC_nnn'GTG
Cuts at: 0 4510 5569 6123
Size: 4510 1059 554
Fragments arranged by size:
4510 1059 554
EaeI y'GGCC_r
Cuts at: 0 2072 3135 4316 6123
Size: 2072 1063 1181 1807
Fragments arranged by size:
2072 1807 1181 1063
EarI CTCTTCn'nnn_
Cuts at: 0 136 1783 2048 2464 2588 3170 3278 3312
Size: 136 1647 265 416 124 582 108 34
Cuts at: 3312 3380 6123
Size: 68 2743
Fragments arranged by size:
2743 1647 582 416 265 136 124 108
68 34
EcoICRI GAG'CTC
Cuts at: 0 417 1407 2957 6123
Size: 417 990 1550 3166
Fragments arranged by size:
3166 1550 990 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 127 136 182 455 878 1190 1291
Size: 117 10 9 46 273 423 312 101
Cuts at: 1291 1556 1764 2128 2332 2445 2719 3483 3632
Size: 265 208 364 204 113 274 764 149
Cuts at: 3632 3774 3890 4078 4471 4666 4697 5039 5261
Size: 142 116 188 393 195 31 342 222
Cuts at: 5261 5567 5627 5771 5892 6011 6123
Size: 306 60 144 121 119 112
Fragments arranged by size:
764 423 393 364 342 312 306 274
273 265 222 208 204 195 188 149
144 142 121 119 117 116 113 112
101 60 46 31 10 9
EcoO109I rG'GnC_Cy
Cuts at: 0 27 319 1753 2770 5136 5514 5515 5854
Size: 27 292 1434 1017 2366 378 1 339
Cuts at: 5854 6123
Size: 269
Fragments arranged by size:
2366 1434 1017 378 339 292 269 27
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3804 6123
Size: 3804 2319
HaeII r_GCGC'y
Cuts at: 0 48 6123
Size: 48 6075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 794 826 1396 1428 1486 1518
Size: 243 32 519 32 570 32 58 32
Cuts at: 1518 1998 2030 2847 2879 3218 3250 3504 3536
Size: 480 32 817 32 339 32 254 32
Cuts at: 3536 3605 3637 4191 4223 4556 4588 5012 5044
Size: 69 32 554 32 333 32 424 32
Cuts at: 5044 6123
Size: 1079
Fragments arranged by size:
1079 817 570 554 519 480 424 339
333 254 243 69 58 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2271 5703 6123
Size: 2271 3432 420
Fragments arranged by size:
3432 2271 420
HindIII A'AGCT_T
Cuts at: 0 3297 6123
Size: 3297 2826
HpaI GTT'AAC
Cuts at: 0 2271 5703 6123
Size: 2271 3432 420
Fragments arranged by size:
3432 2271 420
MfeI C'AATT_G
Cuts at: 0 2159 3432 6123
Size: 2159 1273 2691
Fragments arranged by size:
2691 2159 1273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1740 4554 5898 6123
Size: 1740 2814 1344 225
Fragments arranged by size:
2814 1740 1344 225
MscI TGG'CCA
Cuts at: 0 2074 6123
Size: 2074 4049
MslI CAynn'nnrTG
Cuts at: 0 338 533 1838 2426 2764 2848 3116 4235
Size: 338 195 1305 588 338 84 268 1119
Cuts at: 4235 5734 6109 6123
Size: 1499 375 14
Fragments arranged by size:
1499 1305 1119 588 375 338 338 268
195 84 14
MspA1I CmG'CkG
Cuts at: 0 171 247 1564 1769 2923 3586 3998 4414
Size: 171 76 1317 205 1154 663 412 416
Cuts at: 4414 4720 6123
Size: 306 1403
Fragments arranged by size:
1403 1317 1154 663 416 412 306 205
171 76
NdeI CA'TA_TG
Cuts at: 0 2690 4046 6123
Size: 2690 1356 2077
Fragments arranged by size:
2690 2077 1356
NsiI A_TGCA'T
Cuts at: 0 5469 5721 6123
Size: 5469 252 402
Fragments arranged by size:
5469 402 252
NspI r_CATG'y
Cuts at: 0 37 3568 4997 6123
Size: 37 3531 1429 1126
Fragments arranged by size:
3531 1429 1126 37
PasI CC'CwG_GG
Cuts at: 0 390 6123
Size: 390 5733
PciI A'CATG_T
Cuts at: 0 3564 6123
Size: 3564 2559
PflMI CCAn_nnn'nTGG
Cuts at: 0 295 1842 4777 5419 6123
Size: 295 1547 2935 642 704
Fragments arranged by size:
2935 1547 704 642 295
PfoI T'CCnGG_A
Cuts at: 0 4111 4326 6123
Size: 4111 215 1797
Fragments arranged by size:
4111 1797 215
PpuMI rG'GwC_Cy
Cuts at: 0 319 1753 5854 6123
Size: 319 1434 4101 269
Fragments arranged by size:
4101 1434 319 269
PshAI GACnn'nnGTC
Cuts at: 0 5999 6123
Size: 5999 124
PsiI TTA'TAA
Cuts at: 0 2185 3727 6123
Size: 2185 1542 2396
Fragments arranged by size:
2396 2185 1542
PspOMI G'GGCC_C
Cuts at: 0 26 5514 6123
Size: 26 5488 609
Fragments arranged by size:
5488 609 26
PstI C_TGCA'G
Cuts at: 0 122 629 717 3273 4158 5186 6123
Size: 122 507 88 2556 885 1028 937
Fragments arranged by size:
2556 1028 937 885 507 122 88
PvuII CAG'CTG
Cuts at: 0 171 247 1564 2923 3586 3998 4414 4720
Size: 171 76 1317 1359 663 412 416 306
Cuts at: 4720 6123
Size: 1403
Fragments arranged by size:
1403 1359 1317 663 416 412 306 171
76
SacI G_AGCT'C
Cuts at: 0 419 1409 2959 6123
Size: 419 990 1550 3164
Fragments arranged by size:
3164 1550 990 419
SapI GCTCTTCn'nnn_
Cuts at: 0 1783 6123
Size: 1783 4340
ScaI AGT'ACT
Cuts at: 0 2678 6123
Size: 2678 3445
SexAI A'CCwGG_T
Cuts at: 0 179 321 6123
Size: 179 142 5802
Fragments arranged by size:
5802 179 142
SfcI C'TryA_G
Cuts at: 0 118 625 713 1868 2300 3269 3701 4154
Size: 118 507 88 1155 432 969 432 453
Cuts at: 4154 4592 5124 5182 6123
Size: 438 532 58 941
Fragments arranged by size:
1155 969 941 532 507 453 438 432
432 118 88 58
SmlI C'TyrA_G
Cuts at: 0 2290 2564 5052 6123
Size: 2290 274 2488 1071
Fragments arranged by size:
2488 2290 1071 274
SphI G_CATG'C
Cuts at: 0 37 6123
Size: 37 6086
SspI AAT'ATT
Cuts at: 0 189 213 494 6123
Size: 189 24 281 5629
Fragments arranged by size:
5629 281 189 24
StuI AGG'CCT
Cuts at: 0 440 5204 6123
Size: 440 4764 919
Fragments arranged by size:
4764 919 440
StyI C'CwwG_G
Cuts at: 0 441 1152 3051 3736 4460 5205 6123
Size: 441 711 1899 685 724 745 918
Fragments arranged by size:
1899 918 745 724 711 685 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1044 1923 1930 2261 3858 4121 4942 5365
Size: 1044 879 7 331 1597 263 821 423
Cuts at: 5365 5615 5707 6073 6123
Size: 250 92 366 50
Fragments arranged by size:
1597 1044 879 821 423 366 331 263
250 92 50 7
TatI w'GTAC_w
Cuts at: 0 162 2047 2676 3782 5428 6123
Size: 162 1885 629 1106 1646 695
Fragments arranged by size:
1885 1646 1106 695 629 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1346 1378 5143 5175 5234 5266 5744 5776
Size: 1346 32 3765 32 59 32 478 32
Cuts at: 5776 6123
Size: 347
Fragments arranged by size:
3765 1346 478 347 59 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 5260 6123
Size: 5260 863
XbaI T'CTAG_A
Cuts at: 0 4194 6045 6123
Size: 4194 1851 78
Fragments arranged by size:
4194 1851 78
XmnI GAAnn'nnTTC
Cuts at: 0 1777 2057 3228 3680 4367 5336 6123
Size: 1777 280 1171 452 687 969 787
Fragments arranged by size:
1777 1171 969 787 687 452 280
ZraI GAC'GTC
Cuts at: 0 1425 5825 6001 6123
Size: 1425 4400 176 122
Fragments arranged by size:
4400 1425 176 122
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApoI AseI AvrII BaeI
BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PasI
PciI PflMI PfoI PpuMI PshAI PsiI PspOMI PstI
PvuII SacI SapI ScaI SexAI SfcI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI ApaLI AscI AsiSI AvaI
BbeI BcgI BmtI BplI BsaBI BsiEI BsiWI BspEI
BspHI BsrBI BsrFI BssHII BstZ17I ClaI CspCI DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PmeI PmlI PpiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XcmI
XhoI XmaI