Restriction map for
pF1KB0087
(Linear) (Six-base) MAPSORT of: KIBB0087.seq Check: 8670 from: 1 to: 3420
KIBB0087.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 542 3420
Size: 542 2878
AclI AA'CG_TT
Cuts at: 0 1010 3420
Size: 1010 2410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 1400 2534 2761 3420
Size: 662 738 1134 227 659
Fragments arranged by size:
1134 738 662 659 227
AfeI AGC'GCT
Cuts at: 0 1829 2265 2943 3420
Size: 1829 436 678 477
Fragments arranged by size:
1829 678 477 436
AflIII A'CryG_T
Cuts at: 0 2033 3420
Size: 2033 1387
AhdI GACnn_n'nnGTC
Cuts at: 0 1075 3420
Size: 1075 2345
AleI CACnn'nnGTG
Cuts at: 0 40 722 772 1273 3420
Size: 40 682 50 501 2147
Fragments arranged by size:
2147 682 501 50 40
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 3420
Size: 241 3179
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1908 1940 3420
Size: 1908 32 1480
Fragments arranged by size:
1908 1480 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2127 2280 2421 3048 3420
Size: 2127 153 141 627 372
Fragments arranged by size:
2127 627 372 153 141
ApaI G_GGCC'C
Cuts at: 0 616 1545 3420
Size: 616 929 1875
Fragments arranged by size:
1875 929 616
ApaLI G'TGCA_C
Cuts at: 0 2362 3420
Size: 2362 1058
ApoI r'AATT_y
Cuts at: 0 601 2623 3420
Size: 601 2022 797
Fragments arranged by size:
2022 797 601
AvaI C'yCGr_G
Cuts at: 0 2837 3420
Size: 2837 583
BamHI G'GATC_C
Cuts at: 0 3413 3420
Size: 3413 7
BanI G'GyrC_C
Cuts at: 0 877 1894 2104 3420
Size: 877 1017 210 1316
Fragments arranged by size:
1316 1017 877 210
BanII G_rGCy'C
Cuts at: 0 312 616 674 1545 1577 1847 2528 3420
Size: 312 304 58 871 32 270 681 892
Fragments arranged by size:
892 871 681 312 304 270 58 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 1992 2749 3247 3420
Size: 86 1906 757 498 173
Fragments arranged by size:
1906 757 498 173 86
BciVI GTATCCnnnnn_n'
Cuts at: 0 2519 3420
Size: 2519 901
BclI T'GATC_A
Cuts at: 0 1359 3420
Size: 1359 2061
BglII A'GATC_T
Cuts at: 0 119 2168 3420
Size: 119 2049 1252
Fragments arranged by size:
2049 1252 119
Bme1580I G_kGCm'C
Cuts at: 0 616 1545 1897 2245 2366 2497 3420
Size: 616 929 352 348 121 131 923
Fragments arranged by size:
929 923 616 352 348 131 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 2184 3420
Size: 2184 1236
BmtI G_CTAG'C
Cuts at: 0 2270 3420
Size: 2270 1150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 496 1120 2193 2283 2298 2959 3237
Size: 286 210 624 1073 90 15 661 278
Cuts at: 3237 3420
Size: 183
Fragments arranged by size:
1073 661 624 286 278 210 183 90
15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 789 874 1424 2002 2274 3420
Size: 439 350 85 550 578 272 1146
Fragments arranged by size:
1146 578 550 439 350 272 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 913 980 1440 2329 3420
Size: 343 570 67 460 889 1091
Fragments arranged by size:
1091 889 570 460 343 67
BsaAI yAC'GTr
Cuts at: 0 15 567 3420
Size: 15 552 2853
Fragments arranged by size:
2853 552 15
BsaBI GATnn'nnATC
Cuts at: 0 2057 3420
Size: 2057 1363
BsaWI w'CCGG_w
Cuts at: 0 58 1169 3420
Size: 58 1111 2251
Fragments arranged by size:
2251 1111 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1310 1340 2339 2369 3420
Size: 1310 30 999 30 1051
Fragments arranged by size:
1310 1051 999 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1336 2318 2974 3415 3420
Size: 493 843 982 656 441 5
Fragments arranged by size:
982 843 656 493 441 5
BseYI C'CCAG_C
Cuts at: 0 816 992 1743 1898 2260 2460 3420
Size: 816 176 751 155 362 200 960
Fragments arranged by size:
960 816 751 362 200 176 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1714 2113 3420
Size: 1714 399 1307
Fragments arranged by size:
1714 1307 399
BsiEI CG_ry'CG
Cuts at: 0 77 1875 3420
Size: 77 1798 1545
Fragments arranged by size:
1798 1545 77
BsiHKAI G_wGCw'C
Cuts at: 0 1280 1577 2366 3086 3209 3420
Size: 1280 297 789 720 123 211
Fragments arranged by size:
1280 789 720 297 211 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 406 3420
Size: 406 3014
Bsp1286I G_dGCh'C
Cuts at: 0 312 616 674 1280 1545 1577 1847 1897
Size: 312 304 58 606 265 32 270 50
Cuts at: 1897 2245 2366 2497 2528 3086 3209 3420
Size: 348 121 131 31 558 123 211
Fragments arranged by size:
606 558 348 312 304 270 265 211
131 123 121 58 50 32 31
BspEI T'CCGG_A
Cuts at: 0 1169 3420
Size: 1169 2251
BspHI T'CATG_A
Cuts at: 0 2331 3420
Size: 2331 1089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 821 3420
Size: 233 588 2599
Fragments arranged by size:
2599 588 233
BsrBI CCG'CTC
Cuts at: 0 2964 3237 3420
Size: 2964 273 183
Fragments arranged by size:
2964 273 183
BsrDI GCAATG_nn'
Cuts at: 0 324 1653 2507 3420
Size: 324 1329 854 913
Fragments arranged by size:
1329 913 854 324
BsrFI r'CCGG_y
Cuts at: 0 352 833 3420
Size: 352 481 2587
Fragments arranged by size:
2587 481 352
BssSI C'ACGA_G
Cuts at: 0 108 1189 2232 3420
Size: 108 1081 1043 1188
Fragments arranged by size:
1188 1081 1043 108
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2705 3420
Size: 2705 715
BstEII G'GTnAC_C
Cuts at: 0 129 3117 3420
Size: 129 2988 303
Fragments arranged by size:
2988 303 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1455 2983 3420
Size: 1455 1528 437
Fragments arranged by size:
1528 1455 437
BstYI r'GATC_y
Cuts at: 0 119 894 1559 2117 2168 3220 3413 3420
Size: 119 775 665 558 51 1052 193 7
Fragments arranged by size:
1052 775 665 558 193 119 51 7
Bsu36I CC'TnA_GG
Cuts at: 0 554 1478 1568 3420
Size: 554 924 90 1852
Fragments arranged by size:
1852 924 554 90
BtgI C'CryG_G
Cuts at: 0 312 650 1046 2810 3024 3420
Size: 312 338 396 1764 214 396
Fragments arranged by size:
1764 396 396 338 312 214
BtsI GCAGTG_nn'
Cuts at: 0 1234 2295 2321 3371 3420
Size: 1234 1061 26 1050 49
Fragments arranged by size:
1234 1061 1050 49 26
ClaI AT'CG_AT
Cuts at: 0 2111 3420
Size: 2111 1309
DraIII CAC_nnn'GTG
Cuts at: 0 2705 3420
Size: 2705 715
DrdI GACnn_nn'nnGTC
Cuts at: 0 3126 3420
Size: 3126 294
EaeI y'GGCC_r
Cuts at: 0 74 336 1043 1231 2078 3420
Size: 74 262 707 188 847 1342
Fragments arranged by size:
1342 847 707 262 188 74
EagI C'GGCC_G
Cuts at: 0 74 3420
Size: 74 3346
EarI CTCTTCn'nnn_
Cuts at: 0 844 2345 2510 2822 3091 3420
Size: 844 1501 165 312 269 329
Fragments arranged by size:
1501 844 329 312 269 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 460 3420
Size: 460 2960
EcoICRI GAG'CTC
Cuts at: 0 1575 3420
Size: 1575 1845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 496 662 1120 1400 2193 2283 2298
Size: 286 210 166 458 280 793 90 15
Cuts at: 2298 2534 2761 2959 3237 3420
Size: 236 227 198 278 183
Fragments arranged by size:
793 458 286 280 278 236 227 210
198 183 166 90 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 2977 3388 3420
Size: 418 2559 411 32
Fragments arranged by size:
2559 418 411 32
EcoO109I rG'GnC_Cy
Cuts at: 0 222 549 612 613 1069 1541 1542 2202
Size: 222 327 63 1 456 472 1 660
Cuts at: 2202 3420
Size: 1218
Fragments arranged by size:
1218 660 472 456 327 222 63 1
1
EcoRI G'AATT_C
Cuts at: 0 601 3420
Size: 601 2819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2772 3420
Size: 2772 648
FspI TGC'GCA
Cuts at: 0 2698 3420
Size: 2698 722
HaeII r_GCGC'y
Cuts at: 0 1831 2077 2267 2945 3420
Size: 1831 246 190 678 475
Fragments arranged by size:
1831 678 475 246 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 1061 1093 1551 1583 1746 1778
Size: 104 32 925 32 458 32 163 32
Cuts at: 1778 3420
Size: 1642
Fragments arranged by size:
1642 925 458 163 104 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1014 2013 3099 3420
Size: 1014 999 1086 321
Fragments arranged by size:
1086 1014 999 321
HindIII A'AGCT_T
Cuts at: 0 644 3420
Size: 644 2776
MscI TGG'CCA
Cuts at: 0 1045 1233 2080 3420
Size: 1045 188 847 1340
Fragments arranged by size:
1340 1045 847 188
MslI CAynn'nnrTG
Cuts at: 0 40 317 722 772 1193 1273 1495 1597
Size: 40 277 405 50 421 80 222 102
Cuts at: 1597 1774 2500 2764 3420
Size: 177 726 264 656
Fragments arranged by size:
726 656 421 405 277 264 222 177
102 80 50 40
MspA1I CmG'CkG
Cuts at: 0 1122 3353 3420
Size: 1122 2231 67
Fragments arranged by size:
2231 1122 67
NaeI GCC'GGC
Cuts at: 0 835 3420
Size: 835 2585
NcoI C'CATG_G
Cuts at: 0 312 650 1046 2810 3420
Size: 312 338 396 1764 610
Fragments arranged by size:
1764 610 396 338 312
NgoMIV G'CCGG_C
Cuts at: 0 833 3420
Size: 833 2587
NheI G'CTAG_C
Cuts at: 0 2266 3420
Size: 2266 1154
NruI TCG'CGA
Cuts at: 0 440 3420
Size: 440 2980
NspI r_CATG'y
Cuts at: 0 171 490 1198 2037 3162 3420
Size: 171 319 708 839 1125 258
Fragments arranged by size:
1125 839 708 319 258 171
PciI A'CATG_T
Cuts at: 0 2033 3420
Size: 2033 1387
PflMI CCAn_nnn'nTGG
Cuts at: 0 1524 1995 3420
Size: 1524 471 1425
Fragments arranged by size:
1524 1425 471
PfoI T'CCnGG_A
Cuts at: 0 263 323 2975 3290 3420
Size: 263 60 2652 315 130
Fragments arranged by size:
2652 315 263 130 60
PpuMI rG'GwC_Cy
Cuts at: 0 222 549 1069 2202 3420
Size: 222 327 520 1133 1218
Fragments arranged by size:
1218 1133 520 327 222
PshAI GACnn'nnGTC
Cuts at: 0 702 3420
Size: 702 2718
PspOMI G'GGCC_C
Cuts at: 0 612 1541 3420
Size: 612 929 1879
Fragments arranged by size:
1879 929 612
PstI C_TGCA'G
Cuts at: 0 1559 2426 3378 3420
Size: 1559 867 952 42
Fragments arranged by size:
1559 952 867 42
PvuII CAG'CTG
Cuts at: 0 1122 3420
Size: 1122 2298
SacI G_AGCT'C
Cuts at: 0 1577 3420
Size: 1577 1843
SapI GCTCTTCn'nnn_
Cuts at: 0 844 3091 3420
Size: 844 2247 329
Fragments arranged by size:
2247 844 329
SfcI C'TryA_G
Cuts at: 0 1532 1555 1977 2422 2572 3334 3374 3420
Size: 1532 23 422 445 150 762 40 46
Fragments arranged by size:
1532 762 445 422 150 46 40 23
SmlI C'TyrA_G
Cuts at: 0 454 804 889 1439 2017 2253 2837 3420
Size: 454 350 85 550 578 236 584 583
Fragments arranged by size:
584 583 578 550 454 350 236 85
SphI G_CATG'C
Cuts at: 0 1198 3420
Size: 1198 2222
StuI AGG'CCT
Cuts at: 0 460 3420
Size: 460 2960
StyI C'CwwG_G
Cuts at: 0 206 312 616 650 912 1046 1814 2161
Size: 206 106 304 34 262 134 768 347
Cuts at: 2161 2747 2810 3420
Size: 586 63 610
Fragments arranged by size:
768 610 586 347 304 262 206 134
106 63 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 973 1735 1912 2287 3040 3420
Size: 973 762 177 375 753 380
Fragments arranged by size:
973 762 753 380 375 177
TatI w'GTAC_w
Cuts at: 0 248 2609 3420
Size: 248 2361 811
Fragments arranged by size:
2361 811 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2339 2371 3420
Size: 2339 32 1049
Fragments arranged by size:
2339 1049 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 331 2233 3420
Size: 331 1902 1187
Fragments arranged by size:
1902 1187 331
XhoI C'TCGA_G
Cuts at: 0 2837 3420
Size: 2837 583
XmnI GAAnn'nnTTC
Cuts at: 0 2402 3420
Size: 2402 1018
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BciVI BclI BglII Bme1580I BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NruI NspI PciI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SfcI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BfrBI BglI BlpI
BmgBI BplI Bpu10I BsaHI BsiWI BsmI BsrGI BssHII
BstBI BstZ17I BtgZI CspCI DraI EcoRV FseI FspAI
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NotI NsiI PacI PasI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XmaI ZraI