Restriction map for
pF1KB0098
(Linear) (Six-base) MAPSORT of: KIBB0098.seq Check: 8871 from: 1 to: 2997
KIBB0098.orf
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AccI GT'mk_AC
Cuts at: 0 2327 2997
Size: 2327 670
Acc65I G'GTAC_C
Cuts at: 0 173 2997
Size: 173 2824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 762 884 1157 1398 1914 2997
Size: 462 300 122 273 241 516 1083
Fragments arranged by size:
1083 516 462 300 273 241 122
AflII C'TTAA_G
Cuts at: 0 1816 2997
Size: 1816 1181
AflIII A'CryG_T
Cuts at: 0 1271 2997
Size: 1271 1726
AhdI GACnn_n'nnGTC
Cuts at: 0 510 2997
Size: 510 2487
AleI CACnn'nnGTG
Cuts at: 0 167 2997
Size: 167 2830
AlwNI CAG_nnn'CTG
Cuts at: 0 1501 2631 2997
Size: 1501 1130 366
Fragments arranged by size:
1501 1130 366
ApaI G_GGCC'C
Cuts at: 0 667 1412 2997
Size: 667 745 1585
Fragments arranged by size:
1585 745 667
ApaLI G'TGCA_C
Cuts at: 0 1368 2997
Size: 1368 1629
ApoI r'AATT_y
Cuts at: 0 334 1772 2151 2997
Size: 334 1438 379 846
Fragments arranged by size:
1438 846 379 334
AvaI C'yCGr_G
Cuts at: 0 105 2997
Size: 105 2892
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1012 1045 2997
Size: 1012 33 1952
Fragments arranged by size:
1952 1012 33
BanI G'GyrC_C
Cuts at: 0 173 1482 2997
Size: 173 1309 1515
Fragments arranged by size:
1515 1309 173
BanII G_rGCy'C
Cuts at: 0 667 677 1114 1146 1304 1338 1412 1981
Size: 667 10 437 32 158 34 74 569
Cuts at: 1981 2641 2662 2997
Size: 660 21 335
Fragments arranged by size:
667 660 569 437 335 158 74 34
32 21 10
BbeI G_GCGC'C
Cuts at: 0 1486 2997
Size: 1486 1511
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 768 1861 2474 2997
Size: 219 549 1093 613 523
Fragments arranged by size:
1093 613 549 523 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2904 2938 2997
Size: 2904 34 59
Fragments arranged by size:
2904 59 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 922 2997
Size: 922 2075
BfrBI ATG'CAT
Cuts at: 0 1609 2997
Size: 1609 1388
BglI GCCn_nnn'nGGC
Cuts at: 0 1325 2997
Size: 1325 1672
BglII A'GATC_T
Cuts at: 0 2817 2997
Size: 2817 180
Bme1580I G_kGCm'C
Cuts at: 0 420 667 1372 1412 2997
Size: 420 247 705 40 1585
Fragments arranged by size:
1585 705 420 247 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2529 2653 2997
Size: 2529 124 344
Fragments arranged by size:
2529 344 124
Bpu10I CC'TnA_GC
Cuts at: 0 671 2997
Size: 671 2326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1967 2997
Size: 1967 1030
BsaI GGTCTCn'nnnn_
Cuts at: 0 2904 2997
Size: 2904 93
BsaAI yAC'GTr
Cuts at: 0 1272 1367 2997
Size: 1272 95 1630
Fragments arranged by size:
1630 1272 95
BsaBI GATnn'nnATC
Cuts at: 0 835 2997
Size: 835 2162
BsaHI Gr'CG_yC
Cuts at: 0 1483 2997
Size: 1483 1514
BsaWI w'CCGG_w
Cuts at: 0 1259 2997
Size: 1259 1738
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1235 1265 1582 1612 2655 2685 2997
Size: 1235 30 317 30 1043 30 312
Fragments arranged by size:
1235 1043 317 312 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1633 1909 2961 2997
Size: 1633 276 1052 36
Fragments arranged by size:
1633 1052 276 36
BseYI C'CCAG_C
Cuts at: 0 27 203 2997
Size: 27 176 2794
Fragments arranged by size:
2794 176 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 847 872 1304 1427 2700 2997
Size: 514 333 25 432 123 1273 297
Fragments arranged by size:
1273 514 432 333 297 123 25
BsiHKAI G_wGCw'C
Cuts at: 0 1304 1338 1372 1508 1981 2641 2997
Size: 1304 34 34 136 473 660 356
Fragments arranged by size:
1304 660 473 356 136 34 34
BsmI GAATG_Cn'
Cuts at: 0 896 953 1609 1611 2997
Size: 896 57 656 2 1386
Fragments arranged by size:
1386 896 656 57 2
Bsp1286I G_dGCh'C
Cuts at: 0 420 667 677 1114 1146 1304 1338 1372
Size: 420 247 10 437 32 158 34 34
Cuts at: 1372 1412 1508 1981 2641 2662 2997
Size: 40 96 473 660 21 335
Fragments arranged by size:
660 473 437 420 335 247 158 96
40 34 34 32 21 10
BspHI T'CATG_A
Cuts at: 0 995 2018 2757 2772 2997
Size: 995 1023 739 15 225
Fragments arranged by size:
1023 995 739 225 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2042 2997
Size: 2042 955
BsrDI GCAATG_nn'
Cuts at: 0 2273 2938 2997
Size: 2273 665 59
Fragments arranged by size:
2273 665 59
BsrFI r'CCGG_y
Cuts at: 0 6 2997
Size: 6 2991
BstAPI GCAn_nnn'nTGC
Cuts at: 0 424 2997
Size: 424 2573
BstEII G'GTnAC_C
Cuts at: 0 246 2997
Size: 246 2751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1866 2739 2997
Size: 1866 873 258
Fragments arranged by size:
1866 873 258
BstYI r'GATC_y
Cuts at: 0 1340 1497 2817 2997
Size: 1340 157 1320 180
Fragments arranged by size:
1340 1320 180 157
BtgI C'CryG_G
Cuts at: 0 2000 2442 2997
Size: 2000 442 555
Fragments arranged by size:
2000 555 442
BtsI GCAGTG_nn'
Cuts at: 0 884 1112 1856 2808 2997
Size: 884 228 744 952 189
Fragments arranged by size:
952 884 744 228 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2663 2698 2997
Size: 2663 35 299
Fragments arranged by size:
2663 299 35
DraI TTT'AAA
Cuts at: 0 1163 2997
Size: 1163 1834
DrdI GACnn_nn'nnGTC
Cuts at: 0 2206 2997
Size: 2206 791
EaeI y'GGCC_r
Cuts at: 0 1317 1568 2445 2997
Size: 1317 251 877 552
Fragments arranged by size:
1317 877 552 251
EarI CTCTTCn'nnn_
Cuts at: 0 40 539 1476 1881 2997
Size: 40 499 937 405 1116
Fragments arranged by size:
1116 937 499 405 40
EcoICRI GAG'CTC
Cuts at: 0 1302 1336 1979 2639 2997
Size: 1302 34 643 660 358
Fragments arranged by size:
1302 660 643 358 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 762 884 1157 1398 1914 2529 2653
Size: 462 300 122 273 241 516 615 124
Cuts at: 2653 2997
Size: 344
Fragments arranged by size:
615 516 462 344 300 273 241 124
122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 116 2653 2774 2997
Size: 116 2537 121 223
Fragments arranged by size:
2537 223 121 116
EcoO109I rG'GnC_Cy
Cuts at: 0 109 1409 1832 2031 2646 2997
Size: 109 1300 423 199 615 351
Fragments arranged by size:
1300 615 423 351 199 109
EcoRV GAT'ATC
Cuts at: 0 1265 2997
Size: 1265 1732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1677 1831 2997
Size: 1677 154 1166
Fragments arranged by size:
1677 1166 154
FseI GG_CCGG'CC
Cuts at: 0 10 2997
Size: 10 2987
HaeII r_GCGC'y
Cuts at: 0 1486 2997
Size: 1486 1511
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 538 570 815 847 916 948
Size: 496 32 10 32 245 32 69 32
Cuts at: 948 1206 1238 1325 1357 1780 1812 1819 1851
Size: 258 32 87 32 423 32 7 32
Cuts at: 1851 2385 2417 2502 2534 2683 2715 2771 2803
Size: 534 32 85 32 149 32 56 32
Cuts at: 2803 2956 2988 2997
Size: 153 32 9
Fragments arranged by size:
534 496 423 258 245 153 149 87
85 69 56 32 32 32 32 32
32 32 32 32 32 32 32 32
10 9 7
HincII GTy'rAC
Cuts at: 0 681 714 2997
Size: 681 33 2283
Fragments arranged by size:
2283 681 33
HpaI GTT'AAC
Cuts at: 0 714 2997
Size: 714 2283
KasI G'GCGC_C
Cuts at: 0 1482 2997
Size: 1482 1515
KpnI G_GTAC'C
Cuts at: 0 177 2997
Size: 177 2820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1249 1847 2997
Size: 279 970 598 1150
Fragments arranged by size:
1150 970 598 279
MscI TGG'CCA
Cuts at: 0 1319 1570 2447 2997
Size: 1319 251 877 550
Fragments arranged by size:
1319 877 550 251
MslI CAynn'nnrTG
Cuts at: 0 167 326 1940 2737 2997
Size: 167 159 1614 797 260
Fragments arranged by size:
1614 797 260 167 159
MspA1I CmG'CkG
Cuts at: 0 15 21 147 978 1044 1884 2851 2997
Size: 15 6 126 831 66 840 967 146
Fragments arranged by size:
967 840 831 146 126 66 15 6
NaeI GCC'GGC
Cuts at: 0 8 2997
Size: 8 2989
NarI GG'CG_CC
Cuts at: 0 1483 2997
Size: 1483 1514
NcoI C'CATG_G
Cuts at: 0 2000 2442 2997
Size: 2000 442 555
Fragments arranged by size:
2000 555 442
NgoMIV G'CCGG_C
Cuts at: 0 6 2997
Size: 6 2991
NsiI A_TGCA'T
Cuts at: 0 1611 2997
Size: 1611 1386
NspI r_CATG'y
Cuts at: 0 2190 2922 2997
Size: 2190 732 75
Fragments arranged by size:
2190 732 75
PasI CC'CwG_GG
Cuts at: 0 2124 2655 2997
Size: 2124 531 342
Fragments arranged by size:
2124 531 342
PfoI T'CCnGG_A
Cuts at: 0 1586 2997
Size: 1586 1411
PmlI CAC'GTG
Cuts at: 0 1272 2997
Size: 1272 1725
PpuMI rG'GwC_Cy
Cuts at: 0 109 1832 2031 2997
Size: 109 1723 199 966
Fragments arranged by size:
1723 966 199 109
PshAI GACnn'nnGTC
Cuts at: 0 253 2997
Size: 253 2744
PspOMI G'GGCC_C
Cuts at: 0 663 1408 2997
Size: 663 745 1589
Fragments arranged by size:
1589 745 663
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1673 1705 2997
Size: 1673 32 1292
Fragments arranged by size:
1673 1292 32
PstI C_TGCA'G
Cuts at: 0 1107 2093 2558 2844 2997
Size: 1107 986 465 286 153
Fragments arranged by size:
1107 986 465 286 153
PvuII CAG'CTG
Cuts at: 0 1044 2851 2997
Size: 1044 1807 146
Fragments arranged by size:
1807 1044 146
SacI G_AGCT'C
Cuts at: 0 1304 1338 1981 2641 2997
Size: 1304 34 643 660 356
Fragments arranged by size:
1304 660 643 356 34
SfcI C'TryA_G
Cuts at: 0 1103 2089 2554 2840 2997
Size: 1103 986 465 286 157
Fragments arranged by size:
1103 986 465 286 157
SfoI GGC'GCC
Cuts at: 0 1484 2997
Size: 1484 1513
SmaI CCC'GGG
Cuts at: 0 107 2997
Size: 107 2890
SmlI C'TyrA_G
Cuts at: 0 1816 1982 2997
Size: 1816 166 1015
Fragments arranged by size:
1816 1015 166
SphI G_CATG'C
Cuts at: 0 2922 2997
Size: 2922 75
SspI AAT'ATT
Cuts at: 0 1433 2997
Size: 1433 1564
StuI AGG'CCT
Cuts at: 0 758 2997
Size: 758 2239
StyI C'CwwG_G
Cuts at: 0 818 1114 1565 2000 2265 2442 2927 2997
Size: 818 296 451 435 265 177 485 70
Fragments arranged by size:
818 485 451 435 296 265 177 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1768 1952 2336 2997
Size: 1768 184 384 661
Fragments arranged by size:
1768 661 384 184
TatI w'GTAC_w
Cuts at: 0 576 2498 2997
Size: 576 1922 499
Fragments arranged by size:
1922 576 499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1235 1267 2655 2687 2997
Size: 1235 32 1388 32 310
Fragments arranged by size:
1388 1235 310 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 813 2323 2997
Size: 813 1510 674
Fragments arranged by size:
1510 813 674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1302 2997
Size: 1302 1695
XmaI C'CCGG_G
Cuts at: 0 105 2997
Size: 105 2892
XmnI GAAnn'nnTTC
Cuts at: 0 1609 1660 1927 2997
Size: 1609 51 267 1070
Fragments arranged by size:
1609 1070 267 51
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AvaI BaeI BanI BanII BbeI
BbsI BcgI BciVI BfrBI BglI BglII Bme1580I BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI
CspCI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FseI HaeII Hin4I HincII HpaI
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PfoI PmlI PpuMI
PshAI PspOMI PsrI PstI PvuII SacI SfcI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AlfI AloI AscI
AseI AsiSI AvrII BamHI BbvCI BclI BlpI BmgBI
BmrI BmtI BplI BsiEI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI
DraIII EagI EciI EcoRI FspI FspAI HindIII MfeI
MluI NdeI NheI NotI NruI PacI PciI PflMI
PmeI PpiI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SwaI XbaI XhoI ZraI