Restriction map for
pF1KB0170
(Linear) (Six-base) MAPSORT of: KIBB0170.seq Check: 214 from: 1 to: 3213
KIBB0170.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3067 3213
Size: 3067 146
AatII G_ACGT'C
Cuts at: 0 340 3213
Size: 340 2873
AccI GT'mk_AC
Cuts at: 0 188 2159 3213
Size: 188 1971 1054
Fragments arranged by size:
1971 1054 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1094 1705 1858 2539 2905 3213
Size: 814 280 611 153 681 366 308
Fragments arranged by size:
814 681 611 366 308 280 153
AflII C'TTAA_G
Cuts at: 0 988 2944 3213
Size: 988 1956 269
Fragments arranged by size:
1956 988 269
AflIII A'CryG_T
Cuts at: 0 1970 3213
Size: 1970 1243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 188 3213
Size: 188 3025
AlwNI CAG_nnn'CTG
Cuts at: 0 411 3213
Size: 411 2802
ApaLI G'TGCA_C
Cuts at: 0 1925 3213
Size: 1925 1288
ApoI r'AATT_y
Cuts at: 0 202 242 601 642 1100 1327 1353 1476
Size: 202 40 359 41 458 227 26 123
Cuts at: 1476 1678 1711 1838 2135 2322 3070 3213
Size: 202 33 127 297 187 748 143
Fragments arranged by size:
748 458 359 297 227 202 202 187
143 127 123 41 40 33 26
AseI AT'TA_AT
Cuts at: 0 736 1255 3213
Size: 736 519 1958
Fragments arranged by size:
1958 736 519
BanII G_rGCy'C
Cuts at: 0 123 1671 3213
Size: 123 1548 1542
Fragments arranged by size:
1548 1542 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 1693 3089 3213
Size: 1693 1396 124
Fragments arranged by size:
1693 1396 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1776 1810 3213
Size: 1776 34 1403
Fragments arranged by size:
1776 1403 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1332 3213
Size: 1332 1881
BclI T'GATC_A
Cuts at: 0 999 1305 3213
Size: 999 306 1908
Fragments arranged by size:
1908 999 306
BfrBI ATG'CAT
Cuts at: 0 1524 1719 2823 2827 3213
Size: 1524 195 1104 4 386
Fragments arranged by size:
1524 1104 386 195 4
Bme1580I G_kGCm'C
Cuts at: 0 1929 3213
Size: 1929 1284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2752 3213
Size: 2752 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 540 2960 3213
Size: 122 418 2420 253
Fragments arranged by size:
2420 418 253 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 721 1717 3213
Size: 721 996 1496
Fragments arranged by size:
1496 996 721
BsaHI Gr'CG_yC
Cuts at: 0 337 3213
Size: 337 2876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1304 1334 3213
Size: 1304 30 1879
Fragments arranged by size:
1879 1304 30
BseYI C'CCAG_C
Cuts at: 0 122 2376 2580 3213
Size: 122 2254 204 633
Fragments arranged by size:
2254 633 204 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 3213
Size: 963 2250
BsiHKAI G_wGCw'C
Cuts at: 0 1671 1929 3213
Size: 1671 258 1284
Fragments arranged by size:
1671 1284 258
BsmI GAATG_Cn'
Cuts at: 0 2488 2584 3213
Size: 2488 96 629
Fragments arranged by size:
2488 629 96
Bsp1286I G_dGCh'C
Cuts at: 0 123 1671 1929 3213
Size: 123 1548 258 1284
Fragments arranged by size:
1548 1284 258 123
BspHI T'CATG_A
Cuts at: 0 1331 3213
Size: 1331 1882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3067 3213
Size: 3067 146
BsrDI GCAATG_nn'
Cuts at: 0 603 682 1526 3213
Size: 603 79 844 1687
Fragments arranged by size:
1687 844 603 79
BsrGI T'GTAC_A
Cuts at: 0 663 1634 3213
Size: 663 971 1579
Fragments arranged by size:
1579 971 663
BstAPI GCAn_nnn'nTGC
Cuts at: 0 31 2590 3213
Size: 31 2559 623
Fragments arranged by size:
2559 623 31
BstBI TT'CG_AA
Cuts at: 0 780 1709 3213
Size: 780 929 1504
Fragments arranged by size:
1504 929 780
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2025 2203 3213
Size: 2025 178 1010
Fragments arranged by size:
2025 1010 178
BstYI r'GATC_y
Cuts at: 0 1566 2040 3213
Size: 1566 474 1173
Fragments arranged by size:
1566 1173 474
BstZ17I GTA'TAC
Cuts at: 0 2160 3213
Size: 2160 1053
BtgI C'CryG_G
Cuts at: 0 108 3213
Size: 108 3105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1643 1678 3213
Size: 1643 35 1535
Fragments arranged by size:
1643 1535 35
DraI TTT'AAA
Cuts at: 0 158 581 899 1098 2267 3213
Size: 158 423 318 199 1169 946
Fragments arranged by size:
1169 946 423 318 199 158
EaeI y'GGCC_r
Cuts at: 0 436 2411 3213
Size: 436 1975 802
Fragments arranged by size:
1975 802 436
EarI CTCTTCn'nnn_
Cuts at: 0 1523 3101 3134 3213
Size: 1523 1578 33 79
Fragments arranged by size:
1578 1523 79 33
EcoICRI GAG'CTC
Cuts at: 0 1669 3213
Size: 1669 1544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1094 1705 1858 2539 2752 2905 3213
Size: 814 280 611 153 681 213 153 308
Fragments arranged by size:
814 681 611 308 280 213 153 153
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3127 3213
Size: 3127 86
EcoO109I rG'GnC_Cy
Cuts at: 0 1047 3213
Size: 1047 2166
EcoRI G'AATT_C
Cuts at: 0 601 1327 1711 3213
Size: 601 726 384 1502
Fragments arranged by size:
1502 726 601 384
EcoRV GAT'ATC
Cuts at: 0 72 3213
Size: 72 3141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2279 2970 3213
Size: 2279 691 243
Fragments arranged by size:
2279 691 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 2727 2759 3213
Size: 1010 32 1685 32 454
Fragments arranged by size:
1685 1010 454 32 32
HincII GTy'rAC
Cuts at: 0 189 1192 1698 2424 2904 3213
Size: 189 1003 506 726 480 309
Fragments arranged by size:
1003 726 506 480 309 189
HindIII A'AGCT_T
Cuts at: 0 1435 3213
Size: 1435 1778
HpaI GTT'AAC
Cuts at: 0 1698 2904 3213
Size: 1698 1206 309
Fragments arranged by size:
1698 1206 309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1791 2085 2321 3213
Size: 441 1350 294 236 892
Fragments arranged by size:
1350 892 441 294 236
MscI TGG'CCA
Cuts at: 0 438 2413 3213
Size: 438 1975 800
Fragments arranged by size:
1975 800 438
MslI CAynn'nnrTG
Cuts at: 0 170 1199 3213
Size: 170 1029 2014
Fragments arranged by size:
2014 1029 170
MspA1I CmG'CkG
Cuts at: 0 45 2380 2449 3213
Size: 45 2335 69 764
Fragments arranged by size:
2335 764 69 45
NcoI C'CATG_G
Cuts at: 0 108 3213
Size: 108 3105
NsiI A_TGCA'T
Cuts at: 0 1526 1721 2825 2829 3213
Size: 1526 195 1104 4 384
Fragments arranged by size:
1526 1104 384 195 4
NspI r_CATG'y
Cuts at: 0 594 670 1974 2827 3213
Size: 594 76 1304 853 386
Fragments arranged by size:
1304 853 594 386 76
PasI CC'CwG_GG
Cuts at: 0 711 3213
Size: 711 2502
PciI A'CATG_T
Cuts at: 0 1970 3213
Size: 1970 1243
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 434 1746 2636 3213
Size: 66 368 1312 890 577
Fragments arranged by size:
1312 890 577 368 66
PfoI T'CCnGG_A
Cuts at: 0 1739 2889 3213
Size: 1739 1150 324
Fragments arranged by size:
1739 1150 324
PshAI GACnn'nnGTC
Cuts at: 0 184 3213
Size: 184 3029
PsiI TTA'TAA
Cuts at: 0 755 1561 3213
Size: 755 806 1652
Fragments arranged by size:
1652 806 755
PstI C_TGCA'G
Cuts at: 0 2190 2730 3213
Size: 2190 540 483
Fragments arranged by size:
2190 540 483
PvuII CAG'CTG
Cuts at: 0 45 2380 2449 3213
Size: 45 2335 69 764
Fragments arranged by size:
2335 764 69 45
SacI G_AGCT'C
Cuts at: 0 1671 3213
Size: 1671 1542
SalI G'TCGA_C
Cuts at: 0 187 3213
Size: 187 3026
ScaI AGT'ACT
Cuts at: 0 146 2406 3213
Size: 146 2260 807
Fragments arranged by size:
2260 807 146
SfcI C'TryA_G
Cuts at: 0 1140 1691 2186 2726 3213
Size: 1140 551 495 540 487
Fragments arranged by size:
1140 551 540 495 487
SmlI C'TyrA_G
Cuts at: 0 137 519 988 2944 2975 3213
Size: 137 382 469 1956 31 238
Fragments arranged by size:
1956 469 382 238 137 31
SphI G_CATG'C
Cuts at: 0 2827 3213
Size: 2827 386
SspI AAT'ATT
Cuts at: 0 909 2181 2465 3213
Size: 909 1272 284 748
Fragments arranged by size:
1272 909 748 284
StyI C'CwwG_G
Cuts at: 0 108 283 674 3213
Size: 108 175 391 2539
Fragments arranged by size:
2539 391 175 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2274 3213
Size: 2274 939
TatI w'GTAC_w
Cuts at: 0 144 663 1634 1944 1967 2404 2875 3213
Size: 144 519 971 310 23 437 471 338
Fragments arranged by size:
971 519 471 437 338 310 144 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1304 1336 3213
Size: 1304 32 1877
Fragments arranged by size:
1877 1304 32
XmnI GAAnn'nnTTC
Cuts at: 0 2542 3213
Size: 2542 671
ZraI GAC'GTC
Cuts at: 0 338 3213
Size: 338 2875
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI BanII BbsI BcgI BciVI BclI
BfrBI Bme1580I BpmI BpuEI BsaI BsaHI BsaXI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstXI BstYI BstZ17I BtgI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HindIII HpaI MmeI MscI MslI
MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI
PshAI PsiI PstI PvuII SacI SalI ScaI SfcI
SmlI SphI SspI StyI TaqII TatI TstI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AloI ApaI
AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstEII Bsu36I BtgZI
BtsI ClaI DraIII DrdI EagI EciI FseI FspI
FspAI HaeII KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI