Restriction map for
pF1KSDB0234
(Linear) (Six-base) MAPSORT of: KIBB0234.seq Check: 6512 from: 1 to: 3609
KIBB0234.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 188 2143 3609
Size: 188 1955 1466
Fragments arranged by size:
1955 1466 188
Acc65I G'GTAC_C
Cuts at: 0 802 2276 3609
Size: 802 1474 1333
Fragments arranged by size:
1474 1333 802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 2955 3227 3287 3574 3609
Size: 351 2604 272 60 287 35
Fragments arranged by size:
2604 351 287 272 60 35
AleI CACnn'nnGTG
Cuts at: 0 1180 2299 2972 3609
Size: 1180 1119 673 637
Fragments arranged by size:
1180 1119 673 637
AlwNI CAG_nnn'CTG
Cuts at: 0 505 1435 1627 1801 3443 3609
Size: 505 930 192 174 1642 166
Fragments arranged by size:
1642 930 505 192 174 166
ApaI G_GGCC'C
Cuts at: 0 2764 3019 3609
Size: 2764 255 590
Fragments arranged by size:
2764 590 255
ApaLI G'TGCA_C
Cuts at: 0 3282 3609
Size: 3282 327
ApoI r'AATT_y
Cuts at: 0 251 1300 3172 3609
Size: 251 1049 1872 437
Fragments arranged by size:
1872 1049 437 251
AvaI C'yCGr_G
Cuts at: 0 1019 3254 3609
Size: 1019 2235 355
Fragments arranged by size:
2235 1019 355
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 638 671 794 827 3609
Size: 638 33 123 33 2782
Fragments arranged by size:
2782 638 123 33 33
BamHI G'GATC_C
Cuts at: 0 1860 3609
Size: 1860 1749
BanI G'GyrC_C
Cuts at: 0 298 802 874 1105 1291 2058 2276 2464
Size: 298 504 72 231 186 767 218 188
Cuts at: 2464 2557 2766 3609
Size: 93 209 843
Fragments arranged by size:
843 767 504 298 231 218 209 188
186 93 72
BanII G_rGCy'C
Cuts at: 0 2764 3019 3609
Size: 2764 255 590
Fragments arranged by size:
2764 590 255
BbeI G_GCGC'C
Cuts at: 0 302 3609
Size: 302 3307
BbsI GAAGACnn'nnnn_
Cuts at: 0 1493 3458 3609
Size: 1493 1965 151
Fragments arranged by size:
1965 1493 151
BbvCI CC'TCA_GC
Cuts at: 0 311 3609
Size: 311 3298
BciVI GTATCCnnnnn_n'
Cuts at: 0 243 393 3609
Size: 243 150 3216
Fragments arranged by size:
3216 243 150
BclI T'GATC_A
Cuts at: 0 374 467 3609
Size: 374 93 3142
Fragments arranged by size:
3142 374 93
BfrBI ATG'CAT
Cuts at: 0 2521 3609
Size: 2521 1088
Bme1580I G_kGCm'C
Cuts at: 0 116 879 1294 2063 2764 3019 3286 3428
Size: 116 763 415 769 701 255 267 142
Cuts at: 3428 3609
Size: 181
Fragments arranged by size:
769 763 701 415 267 255 181 142
116
BmgBI CAC'GTC
Cuts at: 0 1450 3609
Size: 1450 2159
BmrI ACTGGGnnnn_n'
Cuts at: 0 254 935 1334 2011 2766 3609
Size: 254 681 399 677 755 843
Fragments arranged by size:
843 755 681 677 399 254
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 304 3609
Size: 304 3305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1563 1823 1892 2749 2846 3609
Size: 569 994 260 69 857 97 763
Fragments arranged by size:
994 857 763 569 260 97 69
Bpu10I CC'TnA_GC
Cuts at: 0 311 3609
Size: 311 3298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 358 1396 3272 3609
Size: 53 305 1038 1876 337
Fragments arranged by size:
1876 1038 337 305 53
BsaI GGTCTCn'nnnn_
Cuts at: 0 1258 2371 3098 3609
Size: 1258 1113 727 511
Fragments arranged by size:
1258 1113 727 511
BsaAI yAC'GTr
Cuts at: 0 2886 3609
Size: 2886 723
BsaBI GATnn'nnATC
Cuts at: 0 661 2452 3609
Size: 661 1791 1157
Fragments arranged by size:
1791 1157 661
BsaHI Gr'CG_yC
Cuts at: 0 299 3609
Size: 299 3310
BsaWI w'CCGG_w
Cuts at: 0 860 2177 3192 3609
Size: 860 1317 1015 417
Fragments arranged by size:
1317 1015 860 417
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 991 1021 2368 2398 3609
Size: 307 30 654 30 1347 30 1211
Fragments arranged by size:
1347 1211 654 307 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 976 2559 2746 3356 3609
Size: 328 648 1583 187 610 253
Fragments arranged by size:
1583 648 610 328 253 187
BseYI C'CCAG_C
Cuts at: 0 208 3609
Size: 208 3401
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3006 3609
Size: 3006 603
BsiHKAI G_wGCw'C
Cuts at: 0 3286 3602 3609
Size: 3286 316 7
Fragments arranged by size:
3286 316 7
BsmI GAATG_Cn'
Cuts at: 0 795 1536 3609
Size: 795 741 2073
Fragments arranged by size:
2073 795 741
Bsp1286I G_dGCh'C
Cuts at: 0 116 879 1294 2063 2764 3019 3286 3428
Size: 116 763 415 769 701 255 267 142
Cuts at: 3428 3602 3609
Size: 174 7
Fragments arranged by size:
769 763 701 415 267 255 174 142
116 7
BspEI T'CCGG_A
Cuts at: 0 2177 3192 3609
Size: 2177 1015 417
Fragments arranged by size:
2177 1015 417
BspHI T'CATG_A
Cuts at: 0 2444 2688 3609
Size: 2444 244 921
Fragments arranged by size:
2444 921 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 705 3609
Size: 705 2904
BsrFI r'CCGG_y
Cuts at: 0 2461 2718 3114 3609
Size: 2461 257 396 495
Fragments arranged by size:
2461 495 396 257
BsrGI T'GTAC_A
Cuts at: 0 525 3609
Size: 525 3084
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3577 3609
Size: 3577 32
BstEII G'GTnAC_C
Cuts at: 0 1029 3609
Size: 1029 2580
BstYI r'GATC_y
Cuts at: 0 135 501 1128 1860 3609
Size: 135 366 627 732 1749
Fragments arranged by size:
1749 732 627 366 135
BstZ17I GTA'TAC
Cuts at: 0 2144 3609
Size: 2144 1465
Bsu36I CC'TnA_GG
Cuts at: 0 7 1078 3593 3609
Size: 7 1071 2515 16
Fragments arranged by size:
2515 1071 16 7
BtgI C'CryG_G
Cuts at: 0 2084 2468 3609
Size: 2084 384 1141
Fragments arranged by size:
2084 1141 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2442 3609
Size: 2442 1167
BtsI GCAGTG_nn'
Cuts at: 0 2111 2327 3093 3609
Size: 2111 216 766 516
Fragments arranged by size:
2111 766 516 216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1431 1466 3609
Size: 1431 35 2143
Fragments arranged by size:
2143 1431 35
DraI TTT'AAA
Cuts at: 0 756 914 1311 1346 3609
Size: 756 158 397 35 2263
Fragments arranged by size:
2263 756 397 158 35
DraIII CAC_nnn'GTG
Cuts at: 0 981 2886 3609
Size: 981 1905 723
Fragments arranged by size:
1905 981 723
EaeI y'GGCC_r
Cuts at: 0 601 704 2804 3609
Size: 601 103 2100 805
Fragments arranged by size:
2100 805 601 103
EarI CTCTTCn'nnn_
Cuts at: 0 1275 2657 2955 3152 3609
Size: 1275 1382 298 197 457
Fragments arranged by size:
1382 1275 457 298 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 569 1563 1823 1892 2749 2846 2955
Size: 351 218 994 260 69 857 97 109
Cuts at: 2955 3227 3287 3574 3609
Size: 272 60 287 35
Fragments arranged by size:
994 857 351 287 272 260 218 109
97 69 60 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 45 509 3609
Size: 45 464 3100
Fragments arranged by size:
3100 464 45
EcoO109I rG'GnC_Cy
Cuts at: 0 947 1218 1643 2760 3015 3016 3250 3609
Size: 947 271 425 1117 255 1 234 359
Fragments arranged by size:
1117 947 425 359 271 255 234 1
EcoRI G'AATT_C
Cuts at: 0 1300 3172 3609
Size: 1300 1872 437
Fragments arranged by size:
1872 1300 437
EcoRV GAT'ATC
Cuts at: 0 573 3609
Size: 573 3036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3036 3609
Size: 3036 573
HaeII r_GCGC'y
Cuts at: 0 179 302 3045 3609
Size: 179 123 2743 564
Fragments arranged by size:
2743 564 179 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 991 1023 3172 3204 3205 3217
Size: 557 32 402 32 2149 32 1 12
Cuts at: 3217 3237 3249 3270 3302 3609
Size: 20 12 21 32 307
Fragments arranged by size:
2149 557 402 307 32 32 32 32
21 20 12 12 1
HincII GTy'rAC
Cuts at: 0 2115 2259 2322 3609
Size: 2115 144 63 1287
Fragments arranged by size:
2115 1287 144 63
HindIII A'AGCT_T
Cuts at: 0 1372 3412 3609
Size: 1372 2040 197
Fragments arranged by size:
2040 1372 197
HpaI GTT'AAC
Cuts at: 0 2322 3609
Size: 2322 1287
KasI G'GCGC_C
Cuts at: 0 298 3609
Size: 298 3311
KpnI G_GTAC'C
Cuts at: 0 806 2280 3609
Size: 806 1474 1329
Fragments arranged by size:
1474 1329 806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 1259 1838 2366 2797 3609
Size: 290 969 579 528 431 812
Fragments arranged by size:
969 812 579 528 431 290
MscI TGG'CCA
Cuts at: 0 603 2806 3609
Size: 603 2203 803
Fragments arranged by size:
2203 803 603
MslI CAynn'nnrTG
Cuts at: 0 155 1180 2122 2299 2482 2972 3609
Size: 155 1025 942 177 183 490 637
Fragments arranged by size:
1025 942 637 490 183 177 155
NaeI GCC'GGC
Cuts at: 0 2720 3609
Size: 2720 889
NarI GG'CG_CC
Cuts at: 0 299 3609
Size: 299 3310
NcoI C'CATG_G
Cuts at: 0 2468 3609
Size: 2468 1141
NdeI CA'TA_TG
Cuts at: 0 306 3609
Size: 306 3303
NgoMIV G'CCGG_C
Cuts at: 0 2718 3609
Size: 2718 891
NsiI A_TGCA'T
Cuts at: 0 2523 3609
Size: 2523 1086
PflMI CCAn_nnn'nTGG
Cuts at: 0 3443 3609
Size: 3443 166
PmlI CAC'GTG
Cuts at: 0 2886 3609
Size: 2886 723
PpuMI rG'GwC_Cy
Cuts at: 0 947 1218 1643 3250 3609
Size: 947 271 425 1607 359
Fragments arranged by size:
1607 947 425 359 271
PshAI GACnn'nnGTC
Cuts at: 0 3095 3609
Size: 3095 514
PsiI TTA'TAA
Cuts at: 0 1090 3563 3609
Size: 1090 2473 46
Fragments arranged by size:
2473 1090 46
PspOMI G'GGCC_C
Cuts at: 0 2760 3015 3609
Size: 2760 255 594
Fragments arranged by size:
2760 594 255
PspXI vC'TCGA_Gb
Cuts at: 0 1019 3609
Size: 1019 2590
PstI C_TGCA'G
Cuts at: 0 716 3088 3609
Size: 716 2372 521
Fragments arranged by size:
2372 716 521
SanDI GG'GwC_CC
Cuts at: 0 1643 3250 3609
Size: 1643 1607 359
Fragments arranged by size:
1643 1607 359
SapI GCTCTTCn'nnn_
Cuts at: 0 2955 3609
Size: 2955 654
ScaI AGT'ACT
Cuts at: 0 1767 3609
Size: 1767 1842
SfcI C'TryA_G
Cuts at: 0 189 712 2147 3084 3272 3609
Size: 189 523 1435 937 188 337
Fragments arranged by size:
1435 937 523 337 189 188
SfoI GGC'GCC
Cuts at: 0 300 3609
Size: 300 3309
SgrAI Cr'CCGG_yG
Cuts at: 0 2461 3609
Size: 2461 1148
SmlI C'TyrA_G
Cuts at: 0 68 337 1019 1375 3287 3609
Size: 68 269 682 356 1912 322
Fragments arranged by size:
1912 682 356 322 269 68
SpeI A'CTAG_T
Cuts at: 0 2956 3609
Size: 2956 653
SspI AAT'ATT
Cuts at: 0 444 1255 3609
Size: 444 811 2354
Fragments arranged by size:
2354 811 444
StuI AGG'CCT
Cuts at: 0 3187 3609
Size: 3187 422
StyI C'CwwG_G
Cuts at: 0 2468 2994 3609
Size: 2468 526 615
Fragments arranged by size:
2468 615 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1542 1568 3387 3609
Size: 1542 26 1819 222
Fragments arranged by size:
1819 1542 222 26
TatI w'GTAC_w
Cuts at: 0 525 1765 3609
Size: 525 1240 1844
Fragments arranged by size:
1844 1240 525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 3609
Size: 307 32 3270
Fragments arranged by size:
3270 307 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3514 3609
Size: 3514 95
XhoI C'TCGA_G
Cuts at: 0 1019 3609
Size: 1019 2590
XmnI GAAnn'nnTTC
Cuts at: 0 3469 3609
Size: 3469 140
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApaI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrFI BsrGI BstAPI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NsiI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PstI SanDI SapI
ScaI SfcI SfoI SgrAI SmlI SpeI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BcgI BglI
BglII BlpI BmtI BsiEI BsiWI BsmBI BsrBI BsrDI
BssHII BssSI BstBI BstXI ClaI DrdI EagI EciI
EcoICRI FseI FspI FspAI MfeI MluI MspA1I NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PpiI PsrI PvuI PvuII RsrII SacI SacII SalI
SbfI SexAI SfiI SgfI SmaI SnaBI SphI SrfI
SwaI Tth111I XbaI XmaI ZraI