Restriction map for
pF1KB0356
(Linear) (Six-base) MAPSORT of: KIBB0356.seq Check: 3747 from: 1 to: 3204
KIBB0356.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 619 3204
Size: 619 2585
AclI AA'CG_TT
Cuts at: 0 434 3204
Size: 434 2770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1981 3204
Size: 686 1295 1223
Fragments arranged by size:
1295 1223 686
AflIII A'CryG_T
Cuts at: 0 2692 2850 3204
Size: 2692 158 354
Fragments arranged by size:
2692 354 158
AhdI GACnn_n'nnGTC
Cuts at: 0 2364 3204
Size: 2364 840
AleI CACnn'nnGTG
Cuts at: 0 1670 3204
Size: 1670 1534
ApaLI G'TGCA_C
Cuts at: 0 995 2603 3204
Size: 995 1608 601
Fragments arranged by size:
1608 995 601
ApoI r'AATT_y
Cuts at: 0 241 379 1010 1688 1722 2556 2576 2830
Size: 241 138 631 678 34 834 20 254
Cuts at: 2830 2926 3204
Size: 96 278
Fragments arranged by size:
834 678 631 278 254 241 138 96
34 20
AseI AT'TA_AT
Cuts at: 0 124 3204
Size: 124 3080
AvaI C'yCGr_G
Cuts at: 0 1502 3204
Size: 1502 1702
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1292 1325 3204
Size: 1292 33 1879
Fragments arranged by size:
1879 1292 33
BanII G_rGCy'C
Cuts at: 0 2904 3204
Size: 2904 300
BciVI GTATCCnnnnn_n'
Cuts at: 0 1509 2701 3204
Size: 1509 1192 503
Fragments arranged by size:
1509 1192 503
BfrBI ATG'CAT
Cuts at: 0 3196 3204
Size: 3196 8
BglII A'GATC_T
Cuts at: 0 1058 3204
Size: 1058 2146
Bme1580I G_kGCm'C
Cuts at: 0 999 1994 2607 3204
Size: 999 995 613 597
Fragments arranged by size:
999 995 613 597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1633 3204
Size: 1633 1571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 3204
Size: 206 2998
BsaAI yAC'GTr
Cuts at: 0 1029 3204
Size: 1029 2175
BsaBI GATnn'nnATC
Cuts at: 0 3137 3204
Size: 3137 67
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1103 2249 3204
Size: 1103 1146 955
Fragments arranged by size:
1146 1103 955
BsiHKAI G_wGCw'C
Cuts at: 0 999 2607 3204
Size: 999 1608 597
Fragments arranged by size:
1608 999 597
BsmI GAATG_Cn'
Cuts at: 0 109 2914 3032 3204
Size: 109 2805 118 172
Fragments arranged by size:
2805 172 118 109
Bsp1286I G_dGCh'C
Cuts at: 0 999 1994 2607 2904 3204
Size: 999 995 613 297 300
Fragments arranged by size:
999 995 613 300 297
BspHI T'CATG_A
Cuts at: 0 3116 3204
Size: 3116 88
BsrDI GCAATG_nn'
Cuts at: 0 494 2129 3053 3204
Size: 494 1635 924 151
Fragments arranged by size:
1635 924 494 151
BssSI C'ACGA_G
Cuts at: 0 1190 1607 3204
Size: 1190 417 1597
Fragments arranged by size:
1597 1190 417
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2430 3204
Size: 2430 774
BstBI TT'CG_AA
Cuts at: 0 1068 2493 3066 3204
Size: 1068 1425 573 138
Fragments arranged by size:
1425 1068 573 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1268 2050 3204
Size: 1268 782 1154
Fragments arranged by size:
1268 1154 782
BstYI r'GATC_y
Cuts at: 0 332 396 453 1058 1611 1821 3170 3204
Size: 332 64 57 605 553 210 1349 34
Fragments arranged by size:
1349 605 553 332 210 64 57 34
BstZ17I GTA'TAC
Cuts at: 0 620 3204
Size: 620 2584
Bsu36I CC'TnA_GG
Cuts at: 0 2290 3204
Size: 2290 914
BtgI C'CryG_G
Cuts at: 0 1083 3204
Size: 1083 2121
BtsI GCAGTG_nn'
Cuts at: 0 1449 2605 3204
Size: 1449 1156 599
Fragments arranged by size:
1449 1156 599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2321 2356 3204
Size: 2321 35 848
Fragments arranged by size:
2321 848 35
DraI TTT'AAA
Cuts at: 0 150 296 755 2012 2403 3204
Size: 150 146 459 1257 391 801
Fragments arranged by size:
1257 801 459 391 150 146
EaeI y'GGCC_r
Cuts at: 0 1336 3204
Size: 1336 1868
EarI CTCTTCn'nnn_
Cuts at: 0 1472 2294 2995 3204
Size: 1472 822 701 209
Fragments arranged by size:
1472 822 701 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1981 3204
Size: 686 1295 1223
Fragments arranged by size:
1295 1223 686
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 3204
Size: 112 3092
EcoO109I rG'GnC_Cy
Cuts at: 0 462 1911 3204
Size: 462 1449 1293
Fragments arranged by size:
1449 1293 462
EcoRI G'AATT_C
Cuts at: 0 1722 3204
Size: 1722 1482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 892 1145 3204
Size: 892 253 2059
Fragments arranged by size:
2059 892 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 324 356 1603 1635 2350 2382 3204
Size: 324 32 1247 32 715 32 822
Fragments arranged by size:
1247 822 715 324 32 32 32
MfeI C'AATT_G
Cuts at: 0 1488 3204
Size: 1488 1716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 3204
Size: 1403 1801
MscI TGG'CCA
Cuts at: 0 1338 3204
Size: 1338 1866
MslI CAynn'nnrTG
Cuts at: 0 370 641 1266 1670 2569 2697 2764 3143
Size: 370 271 625 404 899 128 67 379
Cuts at: 3143 3204
Size: 61
Fragments arranged by size:
899 625 404 379 370 271 128 67
61
NcoI C'CATG_G
Cuts at: 0 1083 3204
Size: 1083 2121
NsiI A_TGCA'T
Cuts at: 0 3198 3204
Size: 3198 6
NspI r_CATG'y
Cuts at: 0 369 1486 2084 2983 3196 3204
Size: 369 1117 598 899 213 8
Fragments arranged by size:
1117 899 598 369 213 8
PpuMI rG'GwC_Cy
Cuts at: 0 462 1911 3204
Size: 462 1449 1293
Fragments arranged by size:
1449 1293 462
PsiI TTA'TAA
Cuts at: 0 988 2840 3204
Size: 988 1852 364
Fragments arranged by size:
1852 988 364
PstI C_TGCA'G
Cuts at: 0 2612 3204
Size: 2612 592
SapI GCTCTTCn'nnn_
Cuts at: 0 1472 3204
Size: 1472 1732
SfcI C'TryA_G
Cuts at: 0 2242 2608 3204
Size: 2242 366 596
Fragments arranged by size:
2242 596 366
SmlI C'TyrA_G
Cuts at: 0 221 3204
Size: 221 2983
SphI G_CATG'C
Cuts at: 0 369 1486 2983 3196 3204
Size: 369 1117 1497 213 8
Fragments arranged by size:
1497 1117 369 213 8
SspI AAT'ATT
Cuts at: 0 426 1041 1864 1930 3204
Size: 426 615 823 66 1274
Fragments arranged by size:
1274 823 615 426 66
StyI C'CwwG_G
Cuts at: 0 1083 3204
Size: 1083 2121
TatI w'GTAC_w
Cuts at: 0 405 1905 3204
Size: 405 1500 1299
Fragments arranged by size:
1500 1299 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 77 109 3204
Size: 77 32 3095
Fragments arranged by size:
3095 77 32
XbaI T'CTAG_A
Cuts at: 0 1726 3204
Size: 1726 1478
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AleI ApaLI ApoI
AseI AvaI BaeI BanII BciVI BfrBI BglII Bme1580I
BplI BpuEI BsaAI BsaBI BseRI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI CspCI DraI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I MfeI MmeI MscI
MslI NcoI NsiI NspI PpuMI PsiI PstI SapI
SfcI SmlI SphI SspI StyI TatI TstI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AlfI AloI
AlwNI ApaI AscI AsiSI AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BclI BglI BlpI BmgBI BmrI
BmtI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BstEII BtgZI ClaI DraIII DrdI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI TaqII Tth111I XcmI
XhoI XmaI XmnI ZraI