(Linear) (Six-base) MAPSORT of: KIBB0401.seq  Check: 275  from: 1  to: 513



KIBB0401.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     513 
   Size:        314     199
 
AlwNI CAG_nnn'CTG

Cuts at:      0     292     513 
   Size:        292     221
 
ApoI r'AATT_y

Cuts at:      0     453     513 
   Size:        453      60
 
BanI G'GyrC_C

Cuts at:      0     343     513 
   Size:        343     170
 
BbvCI CC'TCA_GC

Cuts at:      0      41     513 
   Size:         41     472
 
BclI T'GATC_A

Cuts at:      0     120     513 
   Size:        120     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     513 
   Size:        212     301
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     513 
   Size:         41     472
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     513 
   Size:        384     129
 
BsaBI GATnn'nnATC

Cuts at:      0     200     513 
   Size:        200     313
 
BsrGI T'GTAC_A

Cuts at:      0     422     513 
   Size:        422      91
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     165     513 
   Size:        165     348
 
BstYI r'GATC_y

Cuts at:      0     288     513 
   Size:        288     225
 
BtgI C'CryG_G

Cuts at:      0     383     513 
   Size:        383     130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     314     513 
   Size:        212     102     199

  Fragments arranged by size: 

                 212     199     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0     232     513 
   Size:        232     281
 
HaeII r_GCGC'y

Cuts at:      0      39     513 
   Size:         39     474
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     513 
   Size:        214     299
 
NcoI C'CATG_G

Cuts at:      0     383     513 
   Size:        383     130
 
SmlI C'TyrA_G

Cuts at:      0     363     513 
   Size:        363     150
 
StuI AGG'CCT

Cuts at:      0     106     513 
   Size:        106     407
 
StyI C'CwwG_G

Cuts at:      0     383     513 
   Size:        383     130
 
TatI w'GTAC_w

Cuts at:      0     422     513 
   Size:        422      91
 
XbaI T'CTAG_A

Cuts at:      0     204     513 
   Size:        204     309
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI    BbvCI     BclI     BpmI   Bpu10I
    BpuEI    BsaBI    BsrGI   BstAPI    BstYI     BtgI  Eco57MI EcoO109I
    HaeII     MmeI     NcoI     SmlI     StuI     StyI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI