Restriction map for
pF1KB0407
(Linear) (Six-base) MAPSORT of: KIBB0407.seq Check: 2885 from: 1 to: 1395
KIBB0407.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 511 1060 1395
Size: 511 549 335
Fragments arranged by size:
549 511 335
AatII G_ACGT'C
Cuts at: 0 731 1395
Size: 731 664
Acc65I G'GTAC_C
Cuts at: 0 941 1395
Size: 941 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1395
Size: 894 501
AgeI A'CCGG_T
Cuts at: 0 938 1395
Size: 938 457
AhdI GACnn_n'nnGTC
Cuts at: 0 533 1395
Size: 533 862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 971 1395
Size: 971 424
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1395
Size: 1369 26
AlwNI CAG_nnn'CTG
Cuts at: 0 503 1395
Size: 503 892
ApaLI G'TGCA_C
Cuts at: 0 720 1395
Size: 720 675
ApoI r'AATT_y
Cuts at: 0 619 1395
Size: 619 776
AvaI C'yCGr_G
Cuts at: 0 308 1327 1395
Size: 308 1019 68
Fragments arranged by size:
1019 308 68
BanI G'GyrC_C
Cuts at: 0 941 1395
Size: 941 454
BanII G_rGCy'C
Cuts at: 0 296 739 902 1395
Size: 296 443 163 493
Fragments arranged by size:
493 443 296 163
BbvCI CC'TCA_GC
Cuts at: 0 148 319 1395
Size: 148 171 1076
Fragments arranged by size:
1076 171 148
BglI GCCn_nnn'nGGC
Cuts at: 0 1198 1395
Size: 1198 197
BlpI GC'TnA_GC
Cuts at: 0 92 1395
Size: 92 1303
Bme1580I G_kGCm'C
Cuts at: 0 724 1395
Size: 724 671
BmgBI CAC'GTC
Cuts at: 0 1392 1395
Size: 1392 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 34 1311 1395
Size: 34 1277 84
Fragments arranged by size:
1277 84 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 323 1395
Size: 323 1072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 269 1395
Size: 9 260 1126
Fragments arranged by size:
1126 260 9
Bpu10I CC'TnA_GC
Cuts at: 0 148 319 1395
Size: 148 171 1076
Fragments arranged by size:
1076 171 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1395
Size: 61 1334
BsaI GGTCTCn'nnnn_
Cuts at: 0 207 1395
Size: 207 1188
BsaAI yAC'GTr
Cuts at: 0 719 771 1395
Size: 719 52 624
Fragments arranged by size:
719 624 52
BsaHI Gr'CG_yC
Cuts at: 0 728 1395
Size: 728 667
BsaWI w'CCGG_w
Cuts at: 0 569 938 1395
Size: 569 369 457
Fragments arranged by size:
569 457 369
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 332 362 1395
Size: 213 30 89 30 1033
Fragments arranged by size:
1033 213 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 976 1395
Size: 976 419
BseYI C'CCAG_C
Cuts at: 0 545 1395
Size: 545 850
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 229 352 490 516 635 977 1149
Size: 187 42 123 138 26 119 342 172
Cuts at: 1149 1341 1395
Size: 192 54
Fragments arranged by size:
342 192 187 172 138 123 119 54
42 26
BsiHKAI G_wGCw'C
Cuts at: 0 642 724 902 1395
Size: 642 82 178 493
Fragments arranged by size:
642 493 178 82
BsmI GAATG_Cn'
Cuts at: 0 246 589 1395
Size: 246 343 806
Fragments arranged by size:
806 343 246
Bsp1286I G_dGCh'C
Cuts at: 0 296 642 724 739 902 1395
Size: 296 346 82 15 163 493
Fragments arranged by size:
493 346 296 163 82 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 403 511 1060 1395
Size: 403 108 549 335
Fragments arranged by size:
549 403 335 108
BsrBI CCG'CTC
Cuts at: 0 288 1015 1395
Size: 288 727 380
Fragments arranged by size:
727 380 288
BsrDI GCAATG_nn'
Cuts at: 0 1071 1395
Size: 1071 324
BsrFI r'CCGG_y
Cuts at: 0 938 1395
Size: 938 457
BstAPI GCAn_nnn'nTGC
Cuts at: 0 503 719 1395
Size: 503 216 676
Fragments arranged by size:
676 503 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 545 952 1395
Size: 545 407 443
Fragments arranged by size:
545 443 407
Bsu36I CC'TnA_GG
Cuts at: 0 1149 1395
Size: 1149 246
BtgI C'CryG_G
Cuts at: 0 30 217 1395
Size: 30 187 1178
Fragments arranged by size:
1178 187 30
BtsI GCAGTG_nn'
Cuts at: 0 597 1137 1395
Size: 597 540 258
Fragments arranged by size:
597 540 258
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 757 792 1395
Size: 757 35 603
Fragments arranged by size:
757 603 35
DraI TTT'AAA
Cuts at: 0 630 1395
Size: 630 765
DraIII CAC_nnn'GTG
Cuts at: 0 207 1395
Size: 207 1188
EarI CTCTTCn'nnn_
Cuts at: 0 920 1395
Size: 920 475
EcoICRI GAG'CTC
Cuts at: 0 900 1395
Size: 900 495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 269 894 1395
Size: 9 260 625 501
Fragments arranged by size:
625 501 260 9
EcoO109I rG'GnC_Cy
Cuts at: 0 454 1395
Size: 454 941
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 213 245 519 551 1395
Size: 213 32 274 32 844
Fragments arranged by size:
844 274 213 32 32
KpnI G_GTAC'C
Cuts at: 0 945 1395
Size: 945 450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1395
Size: 679 716
MslI CAynn'nnrTG
Cuts at: 0 1297 1395
Size: 1297 98
MspA1I CmG'CkG
Cuts at: 0 400 1259 1395
Size: 400 859 136
Fragments arranged by size:
859 400 136
NcoI C'CATG_G
Cuts at: 0 30 1395
Size: 30 1365
NspI r_CATG'y
Cuts at: 0 1296 1395
Size: 1296 99
PasI CC'CwG_GG
Cuts at: 0 531 604 1395
Size: 531 73 791
Fragments arranged by size:
791 531 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 400 1395
Size: 400 995
PmlI CAC'GTG
Cuts at: 0 771 1395
Size: 771 624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 1395
Size: 1146 32 217
Fragments arranged by size:
1146 217 32
PspXI vC'TCGA_Gb
Cuts at: 0 1327 1395
Size: 1327 68
PstI C_TGCA'G
Cuts at: 0 508 977 1395
Size: 508 469 418
Fragments arranged by size:
508 469 418
PvuII CAG'CTG
Cuts at: 0 400 1395
Size: 400 995
SacI G_AGCT'C
Cuts at: 0 902 1395
Size: 902 493
SapI GCTCTTCn'nnn_
Cuts at: 0 920 1395
Size: 920 475
SbfI CC_TGCA'GG
Cuts at: 0 508 1395
Size: 508 887
SfcI C'TryA_G
Cuts at: 0 504 973 1170 1395
Size: 504 469 197 225
Fragments arranged by size:
504 469 225 197
SmlI C'TyrA_G
Cuts at: 0 76 1327 1395
Size: 76 1251 68
Fragments arranged by size:
1251 76 68
SpeI A'CTAG_T
Cuts at: 0 610 1395
Size: 610 785
SphI G_CATG'C
Cuts at: 0 1296 1395
Size: 1296 99
StuI AGG'CCT
Cuts at: 0 1168 1395
Size: 1168 227
StyI C'CwwG_G
Cuts at: 0 30 1395
Size: 30 1365
TatI w'GTAC_w
Cuts at: 0 932 1395
Size: 932 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 332 364 564 596 1395
Size: 332 32 200 32 799
Fragments arranged by size:
799 332 200 32 32
XhoI C'TCGA_G
Cuts at: 0 1327 1395
Size: 1327 68
ZraI GAC'GTC
Cuts at: 0 729 1395
Size: 729 666
Enzymes that do cut:
AarI AatII Acc65I AcuI AgeI AhdI AlfI AloI
AlwNI ApaLI ApoI AvaI BanI BanII BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstXI Bsu36I BtgI BtsI CspCI DraI DraIII EarI
EcoICRI Eco57MI EcoO109I Hin4I KpnI MmeI MslI MspA1I
NcoI NspI PasI PflMI PmlI PpiI PspXI PstI
PvuII SacI SapI SbfI SfcI SmlI SpeI SphI
StuI StyI TatI TstI XhoI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AleI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BmtI BsaBI BsiEI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII
BstYI BstZ17I BtgZI ClaI DrdI EaeI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PpuMI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI
TaqII Tth111I XbaI XcmI XmaI XmnI